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Protein

Gelsolin

Gene

Gsn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi469Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi470Calcium 1By similarity1
Metal bindingi500Calcium 1By similarity1
Metal bindingi549Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi589Calcium 2By similarity1
Metal bindingi589Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi590Calcium 2By similarity1
Metal bindingi612Calcium 2By similarity1
Metal bindingi694Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi695Calcium 3By similarity1
Metal bindingi717Calcium 3By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin capping, Actin-binding
Biological processCilium biogenesis/degradation
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-264870 Caspase-mediated cleavage of cytoskeletal proteins
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Gelsolin
Alternative name(s):
Actin-depolymerizing factor
Short name:
ADF
Brevin
Gene namesi
Name:Gsn
Synonyms:Gsb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95851 Gsn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000003638726 – 780GelsolinAdd BLAST755

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei84PhosphotyrosineBy similarity1
Disulfide bondi213 ↔ 226In isoform 1By similarity
Modified residuei407PhosphotyrosineBy similarity1
Modified residuei463PhosphotyrosineBy similarity1
Modified residuei582N6-acetyllysineCombined sources1
Modified residuei601PhosphotyrosineBy similarity1
Modified residuei649PhosphotyrosineBy similarity1
Modified residuei740PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues in vitro.By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP13020
PaxDbiP13020
PeptideAtlasiP13020
PRIDEiP13020

2D gel databases

REPRODUCTION-2DPAGEiP13020

PTM databases

iPTMnetiP13020
PhosphoSitePlusiP13020
SwissPalmiP13020

Expressioni

Gene expression databases

BgeeiENSMUSG00000026879 Expressed in 335 organ(s), highest expression level in skin of back
CleanExiMM_GSN
ExpressionAtlasiP13020 baseline and differential
GenevisibleiP13020 MM

Interactioni

Subunit structurei

Binds to actin and to fibronectin. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X (By similarity). Interacts with the inactive form of EIF2AK2/PKR.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230683, 11 interactors
CORUMiP13020
DIPiDIP-31941N
IntActiP13020, 16 interactors
MINTiP13020
STRINGi10090.ENSMUSP00000028239

Structurei

Secondary structure

1780
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP13020
SMRiP13020
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13020

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati74 – 124Gelsolin-like 1Add BLAST51
Repeati196 – 236Gelsolin-like 2Add BLAST41
Repeati312 – 354Gelsolin-like 3Add BLAST43
Repeati451 – 502Gelsolin-like 4Add BLAST52
Repeati574 – 614Gelsolin-like 5Add BLAST41
Repeati677 – 719Gelsolin-like 6Add BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 174Actin-severingSequence analysisAdd BLAST124
Regioni121 – 124Actin-actin interfilament contact point4
Regioni160 – 167Polyphosphoinositide bindingBy similarity8
Regioni186 – 194Polyphosphoinositide bindingBy similarity9
Regioni432 – 780Actin-binding, Ca-sensitiveSequence analysisAdd BLAST349

Sequence similaritiesi

Belongs to the villin/gelsolin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG0443 Eukaryota
ENOG410XR0A LUCA
GeneTreeiENSGT00760000119111
HOGENOMiHOG000233630
HOVERGENiHBG004183
InParanoidiP13020
KOiK05768
OMAiEWPQTPD
OrthoDBiEOG091G05SC
PhylomeDBiP13020
TreeFamiTF313468

Family and domain databases

Gene3Di3.40.20.10, 6 hits
InterProiView protein in InterPro
IPR029006 ADF-H/Gelsolin-like_dom_sf
IPR030004 Gelsolin
IPR007123 Gelsolin-like_dom
IPR007122 Villin/Gelsolin
PANTHERiPTHR11977 PTHR11977, 1 hit
PTHR11977:SF29 PTHR11977:SF29, 1 hit
PfamiView protein in Pfam
PF00626 Gelsolin, 6 hits
PRINTSiPR00597 GELSOLIN
SMARTiView protein in SMART
SM00262 GEL, 6 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P13020-1) [UniParc]FASTAAdd to basket
Also known as: Secreted, Plasma

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAPYRSSLLC ALLLLALCAL SPSHAATTSR GRAQERAPQS RVSEARPSTM
60 70 80 90 100
VVEHPEFLKA GKEPGLQIWR VEKFDLVPVP PNLYGDFFTG DAYVILKTVQ
110 120 130 140 150
LRNGNLQYDL HYWLGNECSQ DESGAAAIFT VQLDDYLNGR AVQHREVQGF
160 170 180 190 200
ESSTFSGYFK SGLKYKKGGV ASGFKHVVPN EVVVQRLFQV KGRRVVRATE
210 220 230 240 250
VPVSWDSFNN GDCFILDLGN NIYQWCGSGS NKFERLKATQ VSKGIRDNER
260 270 280 290 300
SGRAQVHVSE EGGEPEAMLQ VLGPKPALPE GTEDTAKEDA ANRRLAKLYK
310 320 330 340 350
VSNGAGSMSV SLVADENPFA QGALRSEDCF ILDHGRDGKI FVWKGKQANM
360 370 380 390 400
EERKAALKTA SDFISKMQYP RQTQVSVLPE GGETPLFKQF FKNWRDPDQT
410 420 430 440 450
DGPGLGYLSS HIANVERVPF DAATLHTSTA MAAQHGMDDD GTGQKQIWRI
460 470 480 490 500
EGSNKVPVDP ATYGQFYGGD SYIILYNYRH GGRQGQIIYN WQGAQSTQDE
510 520 530 540 550
VAASAILTAQ LDEELGGTPV QSRVVQGKEP AHLMSLFGGK PMIIYKGGTS
560 570 580 590 600
RDGGQTAPAS IRLFQVRASS SGATRAVEVM PKSGALNSND AFVLKTPSAA
610 620 630 640 650
YLWVGAGASE AEKTGAQELL KVLRSQHVQV EEGSEPDAFW EALGGKTAYR
660 670 680 690 700
TSPRLKDKKM DAHPPRLFAC SNRIGRFVIE EVPGELMQED LATDDVMLLD
710 720 730 740 750
TWDQVFVWVG KDSQEEEKTE ALTSAKRYIE TDPANRDRRT PITVVRQGFE
760 770 780
PPSFVGWFLG WDDNYWSVDP LDRALAELAA
Length:780
Mass (Da):85,942
Last modified:February 12, 2003 - v3
Checksum:iAE94297BE457FF2D
GO
Isoform 2 (identifier: P13020-2) [UniParc]FASTAAdd to basket
Also known as: Cytoplasmic

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Note: Initiator Met-1 is removed or contains a N-acetylmethionine at position 1.1 Publication
Show »
Length:731
Mass (Da):80,763
Checksum:i56F6A4964DF1CB04
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A6PWS5A6PWS5_MOUSE
Gelsolin
Gsn
251Annotation score:
A0A0J9YUJ8A0A0J9YUJ8_MOUSE
Gelsolin
Gsn
166Annotation score:
A0A0J9YUQ8A0A0J9YUQ8_MOUSE
Gelsolin
Gsn
568Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti262 – 263GG → ET in AAA37677 (PubMed:2546951).Curated2
Sequence conflicti274P → H in BAC41004 (PubMed:16141072).Curated1
Sequence conflicti294R → K in AAA37677 (PubMed:2546951).Curated1
Sequence conflicti316E → K in BAC41004 (PubMed:16141072).Curated1
Sequence conflicti323A → P in AAA37677 (PubMed:2546951).Curated1
Sequence conflicti423A → G in AAA37677 (PubMed:2546951).Curated1
Sequence conflicti615G → A in AAA37677 (PubMed:2546951).Curated1
Sequence conflicti638A → G in AAA37677 (PubMed:2546951).Curated1
Sequence conflicti648A → S in AAA37677 (PubMed:2546951).Curated1
Sequence conflicti763D → N in AAA37677 (PubMed:2546951).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189601 – 49Missing in isoform 2. 2 PublicationsAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04953 mRNA Translation: AAA37677.1
AK076156 mRNA Translation: BAC36223.1
AK089934 mRNA Translation: BAC41004.1
AK143664 mRNA Translation: BAE25485.1
BC023143 mRNA Translation: AAH23143.2
CCDSiCCDS15960.1 [P13020-1]
CCDS79785.1 [P13020-2]
RefSeqiNP_666232.2, NM_146120.4 [P13020-1]
UniGeneiMm.21109
Mm.443
Mm.488744

Genome annotation databases

EnsembliENSMUST00000028239; ENSMUSP00000028239; ENSMUSG00000026879 [P13020-1]
ENSMUST00000201185; ENSMUSP00000144561; ENSMUSG00000026879 [P13020-2]
GeneIDi227753
KEGGimmu:227753
UCSCiuc008jkc.2 mouse [P13020-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04953 mRNA Translation: AAA37677.1
AK076156 mRNA Translation: BAC36223.1
AK089934 mRNA Translation: BAC41004.1
AK143664 mRNA Translation: BAE25485.1
BC023143 mRNA Translation: AAH23143.2
CCDSiCCDS15960.1 [P13020-1]
CCDS79785.1 [P13020-2]
RefSeqiNP_666232.2, NM_146120.4 [P13020-1]
UniGeneiMm.21109
Mm.443
Mm.488744

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NPHX-ray3.00A439-767[»]
4CBUX-ray1.30G50-174[»]
4CBWX-ray2.50G50-174[»]
4CBXX-ray2.20G50-174[»]
5MVVX-ray1.40G50-174[»]
ProteinModelPortaliP13020
SMRiP13020
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230683, 11 interactors
CORUMiP13020
DIPiDIP-31941N
IntActiP13020, 16 interactors
MINTiP13020
STRINGi10090.ENSMUSP00000028239

PTM databases

iPTMnetiP13020
PhosphoSitePlusiP13020
SwissPalmiP13020

2D gel databases

REPRODUCTION-2DPAGEiP13020

Proteomic databases

MaxQBiP13020
PaxDbiP13020
PeptideAtlasiP13020
PRIDEiP13020

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028239; ENSMUSP00000028239; ENSMUSG00000026879 [P13020-1]
ENSMUST00000201185; ENSMUSP00000144561; ENSMUSG00000026879 [P13020-2]
GeneIDi227753
KEGGimmu:227753
UCSCiuc008jkc.2 mouse [P13020-1]

Organism-specific databases

CTDi2934
MGIiMGI:95851 Gsn

Phylogenomic databases

eggNOGiKOG0443 Eukaryota
ENOG410XR0A LUCA
GeneTreeiENSGT00760000119111
HOGENOMiHOG000233630
HOVERGENiHBG004183
InParanoidiP13020
KOiK05768
OMAiEWPQTPD
OrthoDBiEOG091G05SC
PhylomeDBiP13020
TreeFamiTF313468

Enzyme and pathway databases

ReactomeiR-MMU-264870 Caspase-mediated cleavage of cytoskeletal proteins
R-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiGsn mouse
EvolutionaryTraceiP13020
PROiPR:P13020
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026879 Expressed in 335 organ(s), highest expression level in skin of back
CleanExiMM_GSN
ExpressionAtlasiP13020 baseline and differential
GenevisibleiP13020 MM

Family and domain databases

Gene3Di3.40.20.10, 6 hits
InterProiView protein in InterPro
IPR029006 ADF-H/Gelsolin-like_dom_sf
IPR030004 Gelsolin
IPR007123 Gelsolin-like_dom
IPR007122 Villin/Gelsolin
PANTHERiPTHR11977 PTHR11977, 1 hit
PTHR11977:SF29 PTHR11977:SF29, 1 hit
PfamiView protein in Pfam
PF00626 Gelsolin, 6 hits
PRINTSiPR00597 GELSOLIN
SMARTiView protein in SMART
SM00262 GEL, 6 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGELS_MOUSE
AccessioniPrimary (citable) accession number: P13020
Secondary accession number(s): Q3UPB1, Q8R590
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 12, 2003
Last modified: October 10, 2018
This is version 190 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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