UniProtKB - P13002 (ELF1_DROME)
Protein
Protein grainyhead
Gene
grh
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Binds a CNS-specific regulatory element of the Dopa decarboxylase (Ddc) gene. Also interacts with sequences adjacent to other transcription units, including Ultrabithorax (Ubx) and engrailed (en). Activity in vivo may be required only at high levels transiently to activate the expression of Ddc in the CNS.2 Publications
GO - Molecular functioni
- DNA binding Source: FlyBase
- DNA-binding transcription factor activity Source: FlyBase
- protein homodimerization activity Source: FlyBase
- sequence-specific DNA binding Source: FlyBase
GO - Biological processi
- chitin-based cuticle development Source: FlyBase
- chitin-based embryonic cuticle biosynthetic process Source: FlyBase
- epithelial cell morphogenesis Source: FlyBase
- fibroblast growth factor receptor signaling pathway Source: FlyBase
- ganglion mother cell fate determination Source: FlyBase
- membrane organization Source: FlyBase
- nervous system development Source: FlyBase
- open tracheal system development Source: FlyBase
- positive regulation of transcription by RNA polymerase II Source: FlyBase
- R3/R4 cell fate commitment Source: FlyBase
- regulation of cell population proliferation Source: FlyBase
- regulation of neurogenesis Source: FlyBase
- regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of tube length, open tracheal system Source: FlyBase
- regulation of wound healing Source: FlyBase
- rhabdomere development Source: FlyBase
- tracheal outgrowth, open tracheal system Source: FlyBase
- ventral cord development Source: FlyBase
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
SignaLinki | P13002 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein grainyheadAlternative name(s): DNA-binding protein ELF-1 Element I-binding activity Protein grainy-head Transcription factor NTF-1 |
Gene namesi | Name:grh Synonyms:elf1 ORF Names:CG5058 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0259211, grh |
Subcellular locationi
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086952 | 1 – 1333 | Protein grainyheadAdd BLAST | 1333 |
Proteomic databases
PaxDbi | P13002 |
Expressioni
Tissue specificityi
Restricted, during embryogenesis, to tissues derived from ectoderm, predominantly the central nervous system (CNS) and the epidermis.1 Publication
Gene expression databases
Bgeei | FBgn0259211, Expressed in embryo and 47 other tissues |
ExpressionAtlasi | P13002, differential |
Genevisiblei | P13002, DM |
Interactioni
Binary interactionsi
P13002
With | #Exp. | IntAct |
---|---|---|
Rel [Q94527] | 3 | EBI-497032,EBI-869024 |
GO - Molecular functioni
- protein homodimerization activity Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 62723, 25 interactors |
DIPi | DIP-59591N |
IntActi | P13002, 12 interactors |
STRINGi | 7227.FBpp0288984 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 21 – 36 | His-richAdd BLAST | 16 | |
Compositional biasi | 134 – 137 | Poly-Gly | 4 | |
Compositional biasi | 153 – 195 | Gln-richAdd BLAST | 43 | |
Compositional biasi | 293 – 300 | Poly-Ala | 8 | |
Compositional biasi | 465 – 516 | Gln-richAdd BLAST | 52 | |
Compositional biasi | 748 – 775 | Gln-richAdd BLAST | 28 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4091, Eukaryota |
InParanoidi | P13002 |
PhylomeDBi | P13002 |
Family and domain databases
InterProi | View protein in InterPro IPR007604, CP2 IPR040167, TF_CP2-like |
PANTHERi | PTHR11037, PTHR11037, 2 hits |
Pfami | View protein in Pfam PF04516, CP2, 1 hit |
s (7+)i Sequence
Sequence statusi: Complete.
This entry describes 7 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
This entry has 7 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform JImported (identifier: P13002-6) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSTSTATTSV ITSNELSLSG HAHGHGHAHQ LHQHTHSRLG VGVGVGILSD
60 70 80 90 100
ASLSPIQQGS GGHSGGGNTN SSPLAPNGVP LLTTMHRSPD SPQPELATMT
110 120 130 140 150
NVNVLDLHTD NSKLYDKEAV FIYETPKVVM PADGGGGNNS DEGHAIDARI
160 170 180 190 200
AAQMGNQAQQ QQQQQQQTEH QPLAKIEFDE NQIIRVVGPN GEQQQIISRE
210 220 230 240 250
IINGEHHILS RNEAGEHILT RIVSDPSKLM PNDNAVATAM YNQAQKMNND
260 270 280 290 300
HGQAVYQTSP LPLDASVLHY SGGNDSNVIK TEADIYEDHK KHAAAAAAAA
310 320 330 340 350
GGGSIIYTTS DPNGVNVKQL PHLTVPQKLD PDLYQADKHI DLIYNDGSKT
360 370 380 390 400
VIYSTTDQKS LEIYSGGDIG SLVSDGQVVV QAGLPYATTT GAGGQPVYIV
410 420 430 440 450
ADGALPAGVE EHLQSGKLNG QTTPIDVSGL SQNEIQGFLL GSHPSSSATV
460 470 480 490 500
STTGVVSTTT ISHHQQQQQQ QQQQQQQQQQ QHQQQQQHPG DIVSAAGVGS
510 520 530 540 550
TGSIVSSAAQ QQQQQQLISI KREPEDLRKD PKNGNIAGAA TANGPGSVIT
560 570 580 590 600
QKILHVDAPT ASEADRPSTP SSSINSTENT ESDSQSVSGS ESGSPGARTT
610 620 630 640 650
ATLEMYATTG GTQIYLQTSH PSTASGAGGG AGPAGAAGGG GVSMQAQSPS
660 670 680 690 700
PGPYITANDY GMYTASRLPP GPPPTSTTTF IAEPSYYREY FAPDGQGGYV
710 720 730 740 750
PASTRSLYGD VDVSVSQPGG VVTYEGRFAG SVPPPATTTV LTSVHHHQQQ
760 770 780 790 800
QQQQQQHQQQ QQQQQHHQQQ QHHSQDGKSN GGATPLYAKA ITAAGLTVDL
810 820 830 840 850
PSPDSGIGTD AITPRDQTNI QQSFDYTELC QPGTLIDANG SIPVSVNSIQ
860 870 880 890 900
QRTAVHGSQN SPTTSLVDTS TNGSTRSRPW HDFGRQNDAD KIQIPKIFTN
910 920 930 940 950
VGFRYHLESP ISSSQRREDD RITYINKGQF YGITLEYVHD AEKPIKNTTV
960 970 980 990 1000
KSVIMLMFRE EKSPEDEIKA WQFWHSRQHS VKQRILDADT KNSVGLVGCI
1010 1020 1030 1040 1050
EEVSHNAIAV YWNPLESSAK INIAVQCLST DFSSQKGVKG LPLHVQIDTF
1060 1070 1080 1090 1100
EDPRDTAVFH RGYCQIKVFC DKGAERKTRD EERRAAKRKM TATGRKKLDE
1110 1120 1130 1140 1150
LYHPVTDRSE FYGMQDFAKP PVLFSPAEDM EKVGQLGIGA ATGMTFNPLS
1160 1170 1180 1190 1200
NGNSNSNSHS SLQSFYGHET DSPDLKGASP FLLHGQKVAT PTLKFHNHFP
1210 1220 1230 1240 1250
PDMQTDKKDH ILDQNMLTST PLTDFGPPMK RGRMTPPTSE RVMLYVRQEN
1260 1270 1280 1290 1300
EEVYTPLHVV PPTTIGLLNA IENKYKISTT SINNIYRTNK KGITAKIDDD
1310 1320 1330
MISFYCNEDI FLLEVQQIED DLYDVTLTEL PNQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0B4LFT7 | A0A0B4LFT7_DROME | Grainy head, isoform P | grh BcDNA:LD38807, CG30111, CG5058, Dmel\CG42311, DREB | 1,063 | Annotation score: | ||
A0A0B4K7F6 | A0A0B4K7F6_DROME | Grainy head, isoform O | grh BcDNA:LD38807, CG30111, CG5058, Dmel\CG42311, DREB | 698 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 262 | P → R in CAA33692 (PubMed:2792757).Curated | 1 | |
Sequence conflicti | 415 | S → R in CAA22954 (PubMed:10731137).Curated | 1 | |
Sequence conflicti | 504 | I → V in AAR99118 (Ref. 5) Curated | 1 | |
Sequence conflicti | 610 | G → C in AAK93490 (PubMed:12537569).Curated | 1 | |
Sequence conflicti | 969 | K → R in AAR99118 (Ref. 5) Curated | 1 | |
Sequence conflicti | 999 | C → V in CAA33692 (PubMed:2792757).Curated | 1 | |
Sequence conflicti | 1006 – 1007 | NA → KS in CAA33692 (PubMed:2792757).Curated | 2 | |
Sequence conflicti | 1089 | K → E in AAR99118 (Ref. 5) Curated | 1 | |
Sequence conflicti | 1233 | R → S in AAK93490 (PubMed:12537569).Curated | 1 | |
Sequence conflicti | 1322 | L → R in AAO24937 (PubMed:12537569).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008609 | 1 – 604 | Missing in isoform O and isoform N. 1 PublicationAdd BLAST | 604 | |
Alternative sequenceiVSP_058160 | 2 – 414 | STSTA…EEHLQ → QEATSTTNYALSYTDWMSEP RRGFSLDSLHAGHVESDPQQ QQHHQQLLHHYYSPGDQVKD QGLVLPLNHQQDHHGHHLTA AGLMQAVAAEIQLNEDQDQL HPNANQNSPYPIYSYYRSEG ERANNGSPGADLPW in isoform K. CuratedAdd BLAST | 413 | |
Alternative sequenceiVSP_008611 | 553 – 822 | Missing in isoform H, isoform I and isoform K. 3 PublicationsAdd BLAST | 270 | |
Alternative sequenceiVSP_008612 | 1133 – 1163 | Missing in isoform H, isoform L and isoform O. 2 PublicationsAdd BLAST | 31 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15657 mRNA Translation: CAA33692.1 AE013599 Genomic DNA Translation: AAF57782.3 AE013599 Genomic DNA Translation: AAF57784.3 AE013599 Genomic DNA Translation: AAM68467.2 AE013599 Genomic DNA Translation: AAM68468.2 AE013599 Genomic DNA Translation: AAM68470.2 AE013599 Genomic DNA Translation: AAM68472.2 AY052066 mRNA Translation: AAK93490.1 BT001414 mRNA Translation: AAN71169.1 BT003182 mRNA Translation: AAO24937.1 BT029431 mRNA Translation: ABK57088.1 AL035312 Genomic DNA Translation: CAA22954.1 BT011460 mRNA Translation: AAR99118.1 |
PIRi | S06206 |
RefSeqi | NP_001286551.1, NM_001299622.1 [P13002-1] NP_476842.2, NM_057494.4 [P13002-1] NP_476843.2, NM_057495.4 [P13002-2] NP_476844.2, NM_057496.5 [P13002-3] NP_476845.2, NM_057497.4 [P13002-6] NP_725723.2, NM_166250.4 [P13002-8] NP_725725.1, NM_166252.3 [P13002-4] |
Genome annotation databases
EnsemblMetazoai | FBtr0299705; FBpp0288983; FBgn0259211 [P13002-1] FBtr0299706; FBpp0288984; FBgn0259211 [P13002-6] FBtr0299707; FBpp0288985; FBgn0259211 [P13002-8] FBtr0300538; FBpp0289765; FBgn0259211 [P13002-2] FBtr0300539; FBpp0289766; FBgn0259211 [P13002-3] FBtr0300541; FBpp0289768; FBgn0259211 [P13002-4] FBtr0306625; FBpp0297580; FBgn0259211 [P13002-5] FBtr0345791; FBpp0311781; FBgn0259211 [P13002-1] |
GeneIDi | 37038 |
KEGGi | dme:Dmel_CG42311 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15657 mRNA Translation: CAA33692.1 AE013599 Genomic DNA Translation: AAF57782.3 AE013599 Genomic DNA Translation: AAF57784.3 AE013599 Genomic DNA Translation: AAM68467.2 AE013599 Genomic DNA Translation: AAM68468.2 AE013599 Genomic DNA Translation: AAM68470.2 AE013599 Genomic DNA Translation: AAM68472.2 AY052066 mRNA Translation: AAK93490.1 BT001414 mRNA Translation: AAN71169.1 BT003182 mRNA Translation: AAO24937.1 BT029431 mRNA Translation: ABK57088.1 AL035312 Genomic DNA Translation: CAA22954.1 BT011460 mRNA Translation: AAR99118.1 |
PIRi | S06206 |
RefSeqi | NP_001286551.1, NM_001299622.1 [P13002-1] NP_476842.2, NM_057494.4 [P13002-1] NP_476843.2, NM_057495.4 [P13002-2] NP_476844.2, NM_057496.5 [P13002-3] NP_476845.2, NM_057497.4 [P13002-6] NP_725723.2, NM_166250.4 [P13002-8] NP_725725.1, NM_166252.3 [P13002-4] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 62723, 25 interactors |
DIPi | DIP-59591N |
IntActi | P13002, 12 interactors |
STRINGi | 7227.FBpp0288984 |
Proteomic databases
PaxDbi | P13002 |
Genome annotation databases
EnsemblMetazoai | FBtr0299705; FBpp0288983; FBgn0259211 [P13002-1] FBtr0299706; FBpp0288984; FBgn0259211 [P13002-6] FBtr0299707; FBpp0288985; FBgn0259211 [P13002-8] FBtr0300538; FBpp0289765; FBgn0259211 [P13002-2] FBtr0300539; FBpp0289766; FBgn0259211 [P13002-3] FBtr0300541; FBpp0289768; FBgn0259211 [P13002-4] FBtr0306625; FBpp0297580; FBgn0259211 [P13002-5] FBtr0345791; FBpp0311781; FBgn0259211 [P13002-1] |
GeneIDi | 37038 |
KEGGi | dme:Dmel_CG42311 |
Organism-specific databases
CTDi | 37038 |
FlyBasei | FBgn0259211, grh |
Phylogenomic databases
eggNOGi | KOG4091, Eukaryota |
InParanoidi | P13002 |
PhylomeDBi | P13002 |
Enzyme and pathway databases
SignaLinki | P13002 |
Miscellaneous databases
BioGRID-ORCSi | 37038, 0 hits in 3 CRISPR screens |
GenomeRNAii | 37038 |
PROi | PR:P13002 |
Gene expression databases
Bgeei | FBgn0259211, Expressed in embryo and 47 other tissues |
ExpressionAtlasi | P13002, differential |
Genevisiblei | P13002, DM |
Family and domain databases
InterProi | View protein in InterPro IPR007604, CP2 IPR040167, TF_CP2-like |
PANTHERi | PTHR11037, PTHR11037, 2 hits |
Pfami | View protein in Pfam PF04516, CP2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ELF1_DROME | |
Accessioni | P13002Primary (citable) accession number: P13002 Secondary accession number(s): A0JQ61 Q9V891 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | October 24, 2003 | |
Last modified: | December 2, 2020 | |
This is version 182 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families