UniProtKB - P12998 (BIOF_ECOLI)
Protein
8-amino-7-oxononanoate synthase
Gene
bioF
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide. Can also use pimeloyl-CoA instead of pimeloyl-ACP as substrate, but it is believed that pimeloyl-ACP rather than pimeloyl-CoA is the physiological substrate of BioF.2 Publications
Catalytic activityi
- 6-carboxyhexanoyl-[ACP] + H+ + L-alanine = (S)-8-amino-7-oxononanoate + CO2 + holo-[ACP]2 PublicationsEC:2.3.1.472 Publications
Cofactori
pyridoxal 5'-phosphate1 Publication
Activity regulationi
Competitively inhibited by D-alanine, 8-amino-7-hydroxy-8-phosphonononanoic acid and 2-amino-3-hydroxy-2-methylnonadioic acid.1 Publication
Kineticsi
- KM=25 µM for pimeloyl-CoA (at pH 7.5 and at 30 degrees Celsius)1 Publication
- KM=0.5 µM for L-alanine (at pH 7.5 and at 30 degrees Celsius)1 Publication
: biotin biosynthesis Pathwayi
This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 21 | Substrate1 Publication | 1 | |
Binding sitei | 133 | Substrate1 Publication | 1 | |
Binding sitei | 179 | Pyridoxal phosphate2 Publications | 1 | |
Binding sitei | 207 | Pyridoxal phosphate2 Publications | 1 | |
Binding sitei | 233 | Pyridoxal phosphate1 Publication | 1 | |
Binding sitei | 352 | Substrate1 Publication | 1 |
GO - Molecular functioni
- 8-amino-7-oxononanoate synthase activity Source: EcoCyc
- pyridoxal phosphate binding Source: UniProtKB
GO - Biological processi
- biotin biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Transferase |
Biological process | Biotin biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:7KAPSYN-MONOMER MetaCyc:7KAPSYN-MONOMER |
BRENDAi | 2.3.1.47, 2026 |
SABIO-RKi | P12998 |
UniPathwayi | UPA00078 |
Names & Taxonomyi
Protein namesi | Recommended name: 8-amino-7-oxononanoate synthase1 Publication (EC:2.3.1.472 Publications)Short name: AONS1 Publication Alternative name(s): 7-keto-8-amino-pelargonic acid synthase1 Publication Short name: 7-KAP synthase1 Publication Short name: KAPA synthase1 Publication 8-amino-7-ketopelargonate synthase |
Gene namesi | Name:bioF1 Publication Ordered Locus Names:b0776, JW0759 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Chemistry databases
DrugBanki | DB03160, N-Pyridoxyl-7-Keto-8-Aminopelargonic Acid-5'-Monophosphate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000163811 | 2 – 384 | 8-amino-7-oxononanoate synthaseAdd BLAST | 383 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 236 | N6-(pyridoxal phosphate)lysine2 Publications | 1 |
Proteomic databases
PaxDbi | P12998 |
PRIDEi | P12998 |
Interactioni
Subunit structurei
Homodimer.
3 PublicationsProtein-protein interaction databases
BioGRIDi | 4259953, 19 interactors |
DIPi | DIP-6870N |
IntActi | P12998, 2 interactors |
STRINGi | 511145.b0776 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P12998 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P12998 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 108 – 109 | Pyridoxal phosphate binding2 Publications | 2 |
Sequence similaritiesi
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.Curated
Phylogenomic databases
eggNOGi | COG0156, Bacteria |
HOGENOMi | CLU_015846_11_2_6 |
InParanoidi | P12998 |
PhylomeDBi | P12998 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_01693, BioF_aminotrans_2, 1 hit |
InterProi | View protein in InterPro IPR001917, Aminotrans_II_pyridoxalP_BS IPR004839, Aminotransferase_I/II IPR004723, AONS_Archaea/Proteobacteria IPR022834, AONS_Proteobacteria IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00858, bioF, 1 hit |
PROSITEi | View protein in PROSITE PS00599, AA_TRANSFER_CLASS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P12998-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSWQEKINAA LDARRAADAL RRRYPVAQGA GRWLVADDRQ YLNFSSNDYL
60 70 80 90 100
GLSHHPQIIR AWQQGAEQFG IGSGGSGHVS GYSVVHQALE EELAEWLGYS
110 120 130 140 150
RALLFISGFA ANQAVIAAMM AKEDRIAADR LSHASLLEAA SLSPSQLRRF
160 170 180 190 200
AHNDVTHLAR LLASPCPGQQ MVVTEGVFSM DGDSAPLAEI QQVTQQHNGW
210 220 230 240 250
LMVDDAHGTG VIGEQGRGSC WLQKVKPELL VVTFGKGFGV SGAAVLCSST
260 270 280 290 300
VADYLLQFAR HLIYSTSMPP AQAQALRASL AVIRSDEGDA RREKLAALIT
310 320 330 340 350
RFRAGVQDLP FTLADSCSAI QPLIVGDNSR ALQLAEKLRQ QGCWVTAIRP
360 370 380
PTVPAGTARL RLTLTAAHEM QDIDRLLEVL HGNG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 188 – 189 | AE → R in CAA00968 (Ref. 2) Curated | 2 |
Mass spectrometryi
Molecular mass is 41464.4±4.5 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04423 Genomic DNA Translation: AAA23516.1 A11542 Unassigned DNA Translation: CAA00968.1 U00096 Genomic DNA Translation: AAC73863.1 AP009048 Genomic DNA Translation: BAE76363.1 |
PIRi | D32025, SYECKP |
RefSeqi | NP_415297.1, NC_000913.3 WP_000118826.1, NZ_STEB01000028.1 |
Genome annotation databases
EnsemblBacteriai | AAC73863; AAC73863; b0776 BAE76363; BAE76363; BAE76363 |
GeneIDi | 57728429 945384 |
KEGGi | ecj:JW0759 eco:b0776 |
PATRICi | fig|1411691.4.peg.1502 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04423 Genomic DNA Translation: AAA23516.1 A11542 Unassigned DNA Translation: CAA00968.1 U00096 Genomic DNA Translation: AAC73863.1 AP009048 Genomic DNA Translation: BAE76363.1 |
PIRi | D32025, SYECKP |
RefSeqi | NP_415297.1, NC_000913.3 WP_000118826.1, NZ_STEB01000028.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BS0 | X-ray | 1.65 | A | 1-384 | [»] | |
1DJ9 | X-ray | 2.00 | A | 1-384 | [»] | |
1DJE | X-ray | 1.71 | A | 1-384 | [»] | |
2G6W | X-ray | 2.14 | A | 1-384 | [»] | |
SMRi | P12998 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259953, 19 interactors |
DIPi | DIP-6870N |
IntActi | P12998, 2 interactors |
STRINGi | 511145.b0776 |
Chemistry databases
DrugBanki | DB03160, N-Pyridoxyl-7-Keto-8-Aminopelargonic Acid-5'-Monophosphate |
Proteomic databases
PaxDbi | P12998 |
PRIDEi | P12998 |
Genome annotation databases
EnsemblBacteriai | AAC73863; AAC73863; b0776 BAE76363; BAE76363; BAE76363 |
GeneIDi | 57728429 945384 |
KEGGi | ecj:JW0759 eco:b0776 |
PATRICi | fig|1411691.4.peg.1502 |
Organism-specific databases
EchoBASEi | EB0119 |
Phylogenomic databases
eggNOGi | COG0156, Bacteria |
HOGENOMi | CLU_015846_11_2_6 |
InParanoidi | P12998 |
PhylomeDBi | P12998 |
Enzyme and pathway databases
UniPathwayi | UPA00078 |
BioCyci | EcoCyc:7KAPSYN-MONOMER MetaCyc:7KAPSYN-MONOMER |
BRENDAi | 2.3.1.47, 2026 |
SABIO-RKi | P12998 |
Miscellaneous databases
EvolutionaryTracei | P12998 |
PROi | PR:P12998 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_01693, BioF_aminotrans_2, 1 hit |
InterProi | View protein in InterPro IPR001917, Aminotrans_II_pyridoxalP_BS IPR004839, Aminotransferase_I/II IPR004723, AONS_Archaea/Proteobacteria IPR022834, AONS_Proteobacteria IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00858, bioF, 1 hit |
PROSITEi | View protein in PROSITE PS00599, AA_TRANSFER_CLASS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BIOF_ECOLI | |
Accessioni | P12998Primary (citable) accession number: P12998 Secondary accession number(s): Q2MBJ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | April 7, 2021 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families