UniProtKB - P12982 (PP12_DROME)
Protein
Serine/threonine-protein phosphatase alpha-2 isoform
Gene
Pp1-87B
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Is essential for the regulation of mitotic chromosomal segregation as well as regulation of chromatin condensation during interphase.2 Publications
Catalytic activityi
Cofactori
Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 62 | Manganese 1By similarity | 1 | |
Metal bindingi | 64 | Manganese 1By similarity | 1 | |
Metal bindingi | 90 | Manganese 1By similarity | 1 | |
Metal bindingi | 90 | Manganese 2By similarity | 1 | |
Metal bindingi | 122 | Manganese 2By similarity | 1 | |
Active sitei | 123 | Proton donorBy similarity | 1 | |
Metal bindingi | 171 | Manganese 2By similarity | 1 | |
Metal bindingi | 246 | Manganese 2By similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- myosin phosphatase activity Source: FlyBase
- protein serine/threonine phosphatase activity Source: FlyBase
- protein serine phosphatase activity Source: UniProtKB-EC
- protein threonine phosphatase activity Source: UniProtKB-EC
GO - Biological processi
- adult locomotory behavior Source: FlyBase
- axon guidance Source: FlyBase
- beta-catenin destruction complex disassembly Source: FlyBase
- chromosome condensation Source: FlyBase
- chromosome segregation Source: FlyBase
- circadian rhythm Source: Reactome
- learning or memory Source: FlyBase
- locomotion Source: FlyBase
- mitotic cell cycle Source: FlyBase
- mitotic metaphase plate congression Source: FlyBase
- nervous system development Source: FlyBase
- olfactory learning Source: FlyBase
- oogenesis Source: FlyBase
- positive regulation of canonical Wnt signaling pathway Source: FlyBase
- positive regulation of Ras protein signal transduction Source: FlyBase
- protein dephosphorylation Source: FlyBase
- spindle assembly Source: FlyBase
- visual learning Source: FlyBase
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-DME-350416, Regulation of non-muscle Myosin II R-DME-350480, Activation of non-muscle Myosin II R-DME-538898, Dephosphorylation of TIM |
SignaLinki | P12982 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Pp1-87B ORF Names:CG5650 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0004103, Pp1-87B |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- polytene chromosome Source: FlyBase
- protein phosphatase type 1 complex Source: FlyBase
- PTW/PP1 phosphatase complex Source: InterPro
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058792 | 1 – 302 | Serine/threonine-protein phosphatase alpha-2 isoformAdd BLAST | 302 |
Proteomic databases
PaxDbi | P12982 |
PRIDEi | P12982 |
Expressioni
Developmental stagei
Detected in embryos and larvae (at protein level).2 Publications
Gene expression databases
Bgeei | FBgn0004103, Expressed in imaginal disc and 38 other tissues |
Genevisiblei | P12982, DM |
Interactioni
Subunit structurei
Binary interactionsi
P12982
With | #Exp. | IntAct |
---|---|---|
Nop17l [Q0E9G3] | 3 | EBI-152633,EBI-150380 |
Protein-protein interaction databases
BioGRIDi | 72126, 66 interactors |
DIPi | DIP-18465N |
IntActi | P12982, 42 interactors |
STRINGi | 7227.FBpp0082067 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0374, Eukaryota |
GeneTreei | ENSGT00940000153472 |
HOGENOMi | CLU_004962_0_0_1 |
InParanoidi | P12982 |
OMAi | YLVMESR |
OrthoDBi | 766640at2759 |
PhylomeDBi | P12982 |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR037979, PPP1CA IPR006186, Ser/Thr-sp_prot-phosphatase IPR031675, STPPase_N |
PANTHERi | PTHR11668:SF377, PTHR11668:SF377, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit PF16891, STPPase_N, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P12982-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGDVMNIDSI ISRLLEVRGA RPGKNVQLSE GEIRGLCLKS REIFLSQPIL
60 70 80 90 100
LELEAPLKIC GDIHGQYYDL LRLFEYGGFP PESNYLFLGD YVDRGKQSLE
110 120 130 140 150
TICLLLAYKI KYSENFFLLR GNHECASINR IYGFYDECKR RYSIKLWKTF
160 170 180 190 200
TDCFNCLPVA AIVDEKIFCC HGGLSPDLTS MEQIRRIMRP TDVPDQGLLC
210 220 230 240 250
DLLWSDPDKD TMGWGENDRG VSFTFGAEVV AKFLQKHEFD LICRAHQVVE
260 270 280 290 300
DGYEFFAKRM LVTLFSAPNY CGEFDNAGAM MSVDDTLMCS FQILKPADKR
KK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15583 mRNA Translation: CAA33609.1 S47852 Genomic DNA Translation: AAB23957.1 AE014297 Genomic DNA Translation: AAF54810.1 AY061063 mRNA Translation: AAL28611.1 |
PIRi | S12960, PAFF1A |
RefSeqi | NP_524937.1, NM_080198.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0082595; FBpp0082067; FBgn0004103 |
GeneIDi | 49260 |
KEGGi | dme:Dmel_CG5650 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15583 mRNA Translation: CAA33609.1 S47852 Genomic DNA Translation: AAB23957.1 AE014297 Genomic DNA Translation: AAF54810.1 AY061063 mRNA Translation: AAL28611.1 |
PIRi | S12960, PAFF1A |
RefSeqi | NP_524937.1, NM_080198.3 |
3D structure databases
SMRi | P12982 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 72126, 66 interactors |
DIPi | DIP-18465N |
IntActi | P12982, 42 interactors |
STRINGi | 7227.FBpp0082067 |
Proteomic databases
PaxDbi | P12982 |
PRIDEi | P12982 |
Genome annotation databases
EnsemblMetazoai | FBtr0082595; FBpp0082067; FBgn0004103 |
GeneIDi | 49260 |
KEGGi | dme:Dmel_CG5650 |
Organism-specific databases
CTDi | 49260 |
FlyBasei | FBgn0004103, Pp1-87B |
Phylogenomic databases
eggNOGi | KOG0374, Eukaryota |
GeneTreei | ENSGT00940000153472 |
HOGENOMi | CLU_004962_0_0_1 |
InParanoidi | P12982 |
OMAi | YLVMESR |
OrthoDBi | 766640at2759 |
PhylomeDBi | P12982 |
Enzyme and pathway databases
Reactomei | R-DME-350416, Regulation of non-muscle Myosin II R-DME-350480, Activation of non-muscle Myosin II R-DME-538898, Dephosphorylation of TIM |
SignaLinki | P12982 |
Miscellaneous databases
BioGRID-ORCSi | 49260, 1 hit in 3 CRISPR screens |
ChiTaRSi | Pp1-87B, fly |
GenomeRNAii | 49260 |
PROi | PR:P12982 |
Gene expression databases
Bgeei | FBgn0004103, Expressed in imaginal disc and 38 other tissues |
Genevisiblei | P12982, DM |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR037979, PPP1CA IPR006186, Ser/Thr-sp_prot-phosphatase IPR031675, STPPase_N |
PANTHERi | PTHR11668:SF377, PTHR11668:SF377, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit PF16891, STPPase_N, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PP12_DROME | |
Accessioni | P12982Primary (citable) accession number: P12982 Secondary accession number(s): Q4PIY5, Q9VG75 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | January 1, 1990 | |
Last modified: | February 10, 2021 | |
This is version 196 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families