Myog

Functionⁱ

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts.11 Publications

GO - Molecular functionⁱ

chromatin DNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- 15706034
- 22847234
DNA binding
Source: MGI
Inferred from direct assayⁱ
DNA-binding transcription activator activity, RNA polymerase II-specific
Source: MGI
Inferred from direct assayⁱ
- 24361185
DNA-binding transcription factor activity
Source: UniProtKB
Inferred from direct assayⁱ
- 21465538
E-box binding
Source: UniProtKB
Inferred from direct assayⁱ
- 15706034
- 22847234
protein dimerization activity Source: InterPro
proximal promoter DNA-binding transcription activator activity, RNA polymerase II-specific
Source: NTNU_SB
Inferred from direct assayⁱ
- 17531403
proximal promoter sequence-specific DNA binding
Source: MGI
Inferred from direct assayⁱ
- 20399744
RNA polymerase II proximal promoter sequence-specific DNA binding
Source: NTNU_SB
Inferred from direct assayⁱ
- 17531403
RNA polymerase II regulatory region sequence-specific DNA binding
Source: BHF-UCL
Inferred from direct assayⁱ
- 19783823
sequence-specific DNA binding
Source: MGI
Inferred from direct assayⁱ
- 22638570
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell cycle Source: UniProtKB-KW
cellular response to estradiol stimulus
Source: UniProtKB
Inferred from direct assayⁱ
- 15706034
cellular response to growth factor stimulus
Source: MGI
Inferred from direct assayⁱ
- 15322112
cellular response to lithium ion
Source: MGI
Inferred from direct assayⁱ
- 15673614
cellular response to tumor necrosis factor
Source: MGI
Inferred from direct assayⁱ
- 24349514
mRNA transcription by RNA polymerase II
Source: BHF-UCL
Inferred by curatorⁱ
- 19783823
muscle organ development
Source: MGI
Inferred from genetic interactionⁱ
- 8587605
myotube differentiation
Source: MGI
Inferred from genetic interactionⁱ
- 22106411
negative regulation of cell proliferation
Source: UniProtKB
Inferred from direct assayⁱ
- 22847234
negative regulation of glycolytic process Source: MGI
ossification
Source: MGI
Inferred from mutant phenotypeⁱ
- 8587605
positive regulation of cell cycle arrest
Source: UniProtKB
Inferred from direct assayⁱ
- 22847234
positive regulation of muscle atrophy
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21465538
positive regulation of myoblast differentiation
Source: UniProtKB
Inferred from direct assayⁱ
- 22847234
positive regulation of myoblast fusion
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
positive regulation of myotube differentiation
Source: UniProtKB
Inferred from direct assayⁱ
- 22847234
positive regulation of oxidative phosphorylation Source: MGI
positive regulation of skeletal muscle fiber development
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 8393145
positive regulation of transcription, DNA-templated
Source: MGI
Inferred from direct assayⁱ
- 17904117
positive regulation of transcription by RNA polymerase II
Source: UniProtKB
Inferred from direct assayⁱ
regulation of myoblast fusion
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21465538
regulation of skeletal muscle satellite cell proliferation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21465538
response to denervation involved in regulation of muscle adaptation
Source: UniProtKB
Inferred from direct assayⁱ
- 21465538
response to electrical stimulus involved in regulation of muscle adaptation Source: UniProtKB
response to muscle activity involved in regulation of muscle adaptation Source: UniProtKB
skeletal muscle cell differentiation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
skeletal muscle fiber development
Source: MGI
Inferred from mutant phenotypeⁱ
- 8393145
skeletal muscle tissue development
Source: MGI
Inferred from direct assayⁱ
- 14762206
striated muscle atrophy
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21465538

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, Developmental protein, DNA-binding
Biological process	Cell cycle, Differentiation, Myogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-MMU-375170 CDO in myogenesis

Reactomeⁱ

R-MMU-375170 CDO in myogenesis

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Myogenin Alternative name(s): MYOD1-related protein
Gene namesⁱ	Name:Myog
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 1

Organism-specific databases

MGIⁱ	MGI:97276 Myog

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Display normal myoblast formation during embryogenesis, but show perinatal lethality because of a deficiency during the later stages of skeletal muscle fiber formation. Show no abnormalities for smooth muscles and cardiocytes differentiation. Conditional mutant with expression abrogated in muscle cells from 15.5 or 17.5 dpc are viable, fertil and exhibit no noticeable muscle growth and reduction of myofiber diameter defects but show smaller body size and mass. Conditional mutant in muscle cells of denervated hindlimb muscles show an inhibition of the denervation-dependent reductions in mass, force and atrophy of slow fiber-type soleus muscles, without increased in satellite cell proliferation and fusion.2 Publications

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127376	1 – 224	MyogeninAdd BLAST		224

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	77	Phosphoserine; by CaMK2GBy similarity	1
Modified residueⁱ	79	Phosphoserine; by CaMK2GBy similarity	1
Modified residueⁱ	87	Phosphothreonine; by CaMK2GBy similarity	1

Post-translational modificationⁱ

Phosphorylated by CAMK2G on threonine and serine amino acids in a muscle activity-dependent manner. Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles (By similarity).By similarity

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	P12979
PaxDbⁱ	P12979
PRIDEⁱ	P12979

PTM databases

iPTMnetⁱ	P12979
PhosphoSitePlusⁱ	P12979

Expressionⁱ

Tissue specificityⁱ

Expressed in myoblast cells. Expressed weakly in myotubes (at protein level). Expressed strongly in denervated muscles and in satellite cells isolated from denervated muscles. Expressed weakly in innervated muscle and in satellite cells isolated from innervated muscles.2 Publications

Developmental stageⁱ

Expressed in the myotome of the somites at 8.5 dpc, onward (at protein level). Expressed in proximal region of both the hindlimb and the forelimb at 11.5 dpc, onward. Expressed during muscle maturation between 15 and 17 dpc and decreases thereafter. Not detected within the heart.3 Publications

Inductionⁱ

Up-regulated in denervated muscles (at protein level). Up-regulated during myogenesis in the embryo and in cell culture models of myogenic differentiation via the p38 MAPK signaling pathway.3 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000026459 Expressed in 168 organ(s), highest expression level in muscle of leg
CleanExⁱ	MM_MYOG
Genevisibleⁱ	P12979 MM

Interactionⁱ

Subunit structureⁱ

Homodimer and heterodimer with E12; heterodimerization enhances MYOG DNA-binding and transcriptional activities. Interacts with SMARCA4/BRG1/BAF190A. Interacts (via C-terminal region) with SSRP1 and SUPT16H; the interaction is indicative of an interaction with the FACT complex. nteracts with CSRP3 (By similarity).By similarity3 Publications

GO - Molecular functionⁱ

protein dimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	201674, 7 interactors
CORUMⁱ	P12979
Database of interacting proteins More...DIPⁱ	DIP-29976N
IntActⁱ	P12979, 5 interactors
Molecular INTeraction database More...MINTⁱ	P12979
STRINGⁱ	10090.ENSMUSP00000027730

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P12979
SMRⁱ	P12979
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	81 – 132	bHLHPROSITE-ProRule annotationAdd BLAST		52

Phylogenomic databases

eggNOGⁱ	KOG3960 Eukaryota ENOG4111SED LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063004
HOGENOMⁱ	HOG000234799
HOVERGENⁱ	HBG006429
InParanoidⁱ	P12979
KEGG Orthology (KO) More...KOⁱ	K18483
OMAⁱ	PWACKIC
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0GKD
PhylomeDBⁱ	P12979
TreeFamⁱ	TF316344

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR002546 Basic IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR039704 Myogenic_factor
PANTHERⁱ	PTHR11534 PTHR11534, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01586 Basic, 1 hit PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00520 BASIC, 1 hit SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P12979-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MELYETSPYF YQEPHFYDGE NYLPVHLQGF EPPGYERTEL SLSPEARGPL 
        60         70         80         90        100
EEKGLGTPEH CPGQCLPWAC KVCKRKSVSV DRRRAATLRE KRRLKKVNEA 
       110        120        130        140        150
FEALKRSTLL NPNQRLPKVE ILRSAIQYIE RLQALLSSLN QEERDLRYRG 
       160        170        180        190        200
GGGPQPMVPS ECNSHSASCS PEWGNALEFG PNPGDHLLAA DPTDAHNLHS 
       210        220 
LTSIVDSITV EDMSVAFPDE TMPN

Length:224

Mass (Da):25,203

Last modified:December 1, 1992 - v2

Checksum:ⁱCC7352C1EDF9D3D8

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M95800 Genomic DNA Translation: AAB59676.1 X15784 mRNA Translation: CAA33785.1 Sequence problems. D90156 mRNA Translation: BAA14187.1 BC048683 mRNA Translation: AAH48683.1 BC068019 mRNA Translation: AAH68019.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS15306.1
PIRⁱ	A35882 A36675
NCBI Reference Sequences More...RefSeqⁱ	NP_112466.1, NM_031189.2
UniGeneⁱ	Mm.16528

Genome annotation databases

Ensemblⁱ	ENSMUST00000027730; ENSMUSP00000027730; ENSMUSG00000026459
GeneIDⁱ	17928
KEGGⁱ	mmu:17928
UCSC genome browser More...UCSCⁱ	uc007crl.2 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P12979	Myogenin		HUMAN		224	UniRef90_P12979
Myogenin		BOVIN		224
Myogenin		PIG		224
Myogenin		SAIBB		249
Uncharacterized protein		CALJA		249
+126

Protein	Similar proteins		Species	Score	Length	Source
P12979	Myogenin		HUMAN		224	UniRef50_P12979
Myogenin		PIG		224
Myogenin		BOVIN		224
Myogenin		RAT		287
Myogenin		CHICK		227
+188

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M95800 Genomic DNA Translation: AAB59676.1 X15784 mRNA Translation: CAA33785.1 Sequence problems. D90156 mRNA Translation: BAA14187.1 BC048683 mRNA Translation: AAH48683.1 BC068019 mRNA Translation: AAH68019.1
CCDSⁱ	CCDS15306.1
PIRⁱ	A35882 A36675
RefSeqⁱ	NP_112466.1, NM_031189.2
UniGeneⁱ	Mm.16528

3D structure databases

ProteinModelPortalⁱ	P12979
SMRⁱ	P12979
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	201674, 7 interactors
CORUMⁱ	P12979
DIPⁱ	DIP-29976N
IntActⁱ	P12979, 5 interactors
MINTⁱ	P12979
STRINGⁱ	10090.ENSMUSP00000027730

PTM databases

iPTMnetⁱ	P12979
PhosphoSitePlusⁱ	P12979

Proteomic databases

EPDⁱ	P12979
PaxDbⁱ	P12979
PRIDEⁱ	P12979

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000027730; ENSMUSP00000027730; ENSMUSG00000026459
GeneIDⁱ	17928
KEGGⁱ	mmu:17928
UCSCⁱ	uc007crl.2 mouse

Organism-specific databases

CTDⁱ	4656
MGIⁱ	MGI:97276 Myog

Phylogenomic databases

eggNOGⁱ	KOG3960 Eukaryota ENOG4111SED LUCA
GeneTreeⁱ	ENSGT00530000063004
HOGENOMⁱ	HOG000234799
HOVERGENⁱ	HBG006429
InParanoidⁱ	P12979
KOⁱ	K18483
OMAⁱ	PWACKIC
OrthoDBⁱ	EOG091G0GKD
PhylomeDBⁱ	P12979
TreeFamⁱ	TF316344

Enzyme and pathway databases

Reactomeⁱ	R-MMU-375170 CDO in myogenesis

Reactomeⁱ

R-MMU-375170 CDO in myogenesis

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P12979
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000026459 Expressed in 168 organ(s), highest expression level in muscle of leg
CleanExⁱ	MM_MYOG
Genevisibleⁱ	P12979 MM

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR002546 Basic IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR039704 Myogenic_factor
PANTHERⁱ	PTHR11534 PTHR11534, 1 hit
Pfamⁱ	View protein in Pfam PF01586 Basic, 1 hit PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00520 BASIC, 1 hit SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MYOG_MOUSE
Accessionⁱ	P12979Primary (citable) accession number: P12979
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
	Last sequence update:	December 1, 1992
	Last modified:	November 7, 2018
	This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P12979 (MYOG_MOUSE)

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Myogenin

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ