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Protein

Myogenin

Gene

Myog

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts.11 Publications

GO - Molecular functioni

  • chromatin DNA binding Source: UniProtKB
  • core promoter binding Source: MGI
  • DNA binding Source: MGI
  • DNA binding transcription factor activity Source: UniProtKB
  • E-box binding Source: UniProtKB
  • protein dimerization activity Source: InterPro
  • proximal promoter sequence-specific DNA binding Source: MGI
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: BHF-UCL
  • sequence-specific DNA binding Source: MGI
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: MGI

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cellular response to estradiol stimulus Source: UniProtKB
  • cellular response to growth factor stimulus Source: MGI
  • cellular response to lithium ion Source: MGI
  • cellular response to tumor necrosis factor Source: MGI
  • mRNA transcription by RNA polymerase II Source: BHF-UCL
  • muscle organ development Source: MGI
  • myotube differentiation Source: MGI
  • negative regulation of cell proliferation Source: UniProtKB
  • negative regulation of glycolytic process Source: MGI
  • ossification Source: MGI
  • positive regulation of cell cycle arrest Source: UniProtKB
  • positive regulation of muscle atrophy Source: UniProtKB
  • positive regulation of myoblast differentiation Source: UniProtKB
  • positive regulation of myoblast fusion Source: GO_Central
  • positive regulation of myotube differentiation Source: UniProtKB
  • positive regulation of oxidative phosphorylation Source: MGI
  • positive regulation of skeletal muscle fiber development Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: UniProtKB
  • regulation of myoblast fusion Source: UniProtKB
  • regulation of skeletal muscle satellite cell proliferation Source: UniProtKB
  • response to denervation involved in regulation of muscle adaptation Source: UniProtKB
  • response to electrical stimulus involved in regulation of muscle adaptation Source: UniProtKB
  • response to muscle activity involved in regulation of muscle adaptation Source: UniProtKB
  • skeletal muscle cell differentiation Source: GO_Central
  • skeletal muscle fiber development Source: MGI
  • skeletal muscle tissue development Source: MGI
  • striated muscle atrophy Source: UniProtKB

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processCell cycle, Differentiation, Myogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-375170 CDO in myogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Myogenin
Alternative name(s):
MYOD1-related protein
Gene namesi
Name:Myog
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:97276 Myog

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Display normal myoblast formation during embryogenesis, but show perinatal lethality because of a deficiency during the later stages of skeletal muscle fiber formation. Show no abnormalities for smooth muscles and cardiocytes differentiation. Conditional mutant with expression abrogated in muscle cells from 15.5 or 17.5 dpc are viable, fertil and exhibit no noticeable muscle growth and reduction of myofiber diameter defects but show smaller body size and mass. Conditional mutant in muscle cells of denervated hindlimb muscles show an inhibition of the denervation-dependent reductions in mass, force and atrophy of slow fiber-type soleus muscles, without increased in satellite cell proliferation and fusion.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001273761 – 224MyogeninAdd BLAST224

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei77Phosphoserine; by CaMK2GBy similarity1
Modified residuei79Phosphoserine; by CaMK2GBy similarity1
Modified residuei87Phosphothreonine; by CaMK2GBy similarity1

Post-translational modificationi

Phosphorylated by CAMK2G on threonine and serine amino acids in a muscle activity-dependent manner. Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP12979
PaxDbiP12979
PRIDEiP12979

PTM databases

iPTMnetiP12979
PhosphoSitePlusiP12979

Expressioni

Tissue specificityi

Expressed in myoblast cells. Expressed weakly in myotubes (at protein level). Expressed strongly in denervated muscles and in satellite cells isolated from denervated muscles. Expressed weakly in innervated muscle and in satellite cells isolated from innervated muscles.2 Publications

Developmental stagei

Expressed in the myotome of the somites at 8.5 dpc, onward (at protein level). Expressed in proximal region of both the hindlimb and the forelimb at 11.5 dpc, onward. Expressed during muscle maturation between 15 and 17 dpc and decreases thereafter. Not detected within the heart.3 Publications

Inductioni

Up-regulated in denervated muscles (at protein level). Up-regulated during myogenesis in the embryo and in cell culture models of myogenic differentiation via the p38 MAPK signaling pathway.3 Publications

Gene expression databases

BgeeiENSMUSG00000026459
CleanExiMM_MYOG
GenevisibleiP12979 MM

Interactioni

Subunit structurei

Homodimer and heterodimer with E12; heterodimerization enhances MYOG DNA-binding and transcriptional activities. Interacts with SMARCA4/BRG1/BAF190A. Interacts (via C-terminal region) with SSRP1 and SUPT16H; the interaction is indicative of an interaction with the FACT complex. nteracts with CSRP3 (By similarity).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201674, 7 interactors
CORUMiP12979
DIPiDIP-29976N
IntActiP12979, 5 interactors
MINTiP12979
STRINGi10090.ENSMUSP00000027730

Structurei

3D structure databases

ProteinModelPortaliP12979
SMRiP12979
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 132bHLHPROSITE-ProRule annotationAdd BLAST52

Phylogenomic databases

eggNOGiKOG3960 Eukaryota
ENOG4111SED LUCA
GeneTreeiENSGT00530000063004
HOGENOMiHOG000234799
HOVERGENiHBG006429
InParanoidiP12979
KOiK18483
OMAiPNPGDHL
OrthoDBiEOG091G0GKD
PhylomeDBiP12979
TreeFamiTF316344

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR002546 Basic
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF01586 Basic, 1 hit
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00520 BASIC, 1 hit
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

P12979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELYETSPYF YQEPHFYDGE NYLPVHLQGF EPPGYERTEL SLSPEARGPL
60 70 80 90 100
EEKGLGTPEH CPGQCLPWAC KVCKRKSVSV DRRRAATLRE KRRLKKVNEA
110 120 130 140 150
FEALKRSTLL NPNQRLPKVE ILRSAIQYIE RLQALLSSLN QEERDLRYRG
160 170 180 190 200
GGGPQPMVPS ECNSHSASCS PEWGNALEFG PNPGDHLLAA DPTDAHNLHS
210 220
LTSIVDSITV EDMSVAFPDE TMPN
Length:224
Mass (Da):25,203
Last modified:December 1, 1992 - v2
Checksum:iCC7352C1EDF9D3D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95800 Genomic DNA Translation: AAB59676.1
X15784 mRNA Translation: CAA33785.1 Sequence problems.
D90156 mRNA Translation: BAA14187.1
BC048683 mRNA Translation: AAH48683.1
BC068019 mRNA Translation: AAH68019.1
CCDSiCCDS15306.1
PIRiA35882 A36675
RefSeqiNP_112466.1, NM_031189.2
UniGeneiMm.16528

Genome annotation databases

EnsembliENSMUST00000027730; ENSMUSP00000027730; ENSMUSG00000026459
GeneIDi17928
KEGGimmu:17928
UCSCiuc007crl.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMYOG_MOUSE
AccessioniPrimary (citable) accession number: P12979
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 1, 1992
Last modified: July 18, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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