UniProtKB - P12979 (MYOG_MOUSE)
Protein
Myogenin
Gene
Myog
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts.11 Publications
GO - Molecular functioni
- chromatin DNA binding Source: UniProtKB
- cis-regulatory region sequence-specific DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- E-box binding Source: UniProtKB
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: BHF-UCL
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cellular response to estradiol stimulus Source: UniProtKB
- cellular response to growth factor stimulus Source: MGI
- cellular response to lithium ion Source: MGI
- cellular response to tumor necrosis factor Source: MGI
- muscle cell differentiation Source: ARUK-UCL
- muscle organ development Source: MGI
- myotube differentiation Source: MGI
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of glycolytic process Source: MGI
- ossification Source: MGI
- positive regulation of cell cycle arrest Source: UniProtKB
- positive regulation of muscle atrophy Source: UniProtKB
- positive regulation of myoblast differentiation Source: UniProtKB
- positive regulation of myotube differentiation Source: UniProtKB
- positive regulation of oxidative phosphorylation Source: MGI
- positive regulation of skeletal muscle fiber development Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of myoblast fusion Source: UniProtKB
- regulation of skeletal muscle satellite cell proliferation Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to denervation involved in regulation of muscle adaptation Source: UniProtKB
- response to electrical stimulus involved in regulation of muscle adaptation Source: UniProtKB
- response to muscle activity involved in regulation of muscle adaptation Source: UniProtKB
- skeletal muscle cell differentiation Source: GO_Central
- skeletal muscle fiber development Source: MGI
- skeletal muscle tissue development Source: MGI
- striated muscle atrophy Source: UniProtKB
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Cell cycle, Differentiation, Myogenesis, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-525793, Myogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: MyogeninAlternative name(s): MYOD1-related protein |
Gene namesi | Name:Myog |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97276, Myog |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Note: Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin-remodeling and transcription initiation in developing embryos.
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- protein-DNA complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Display normal myoblast formation during embryogenesis, but show perinatal lethality because of a deficiency during the later stages of skeletal muscle fiber formation. Show no abnormalities for smooth muscles and cardiocytes differentiation. Conditional mutant with expression abrogated in muscle cells from 15.5 or 17.5 dpc are viable, fertil and exhibit no noticeable muscle growth and reduction of myofiber diameter defects but show smaller body size and mass. Conditional mutant in muscle cells of denervated hindlimb muscles show an inhibition of the denervation-dependent reductions in mass, force and atrophy of slow fiber-type soleus muscles, without increased in satellite cell proliferation and fusion.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127376 | 1 – 224 | MyogeninAdd BLAST | 224 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 77 | Phosphoserine; by CaMK2GBy similarity | 1 | |
Modified residuei | 79 | Phosphoserine; by CaMK2GBy similarity | 1 | |
Modified residuei | 87 | Phosphothreonine; by CaMK2GBy similarity | 1 |
Post-translational modificationi
Phosphorylated by CAMK2G on threonine and serine amino acids in a muscle activity-dependent manner. Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles (By similarity).By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P12979 |
PaxDbi | P12979 |
PRIDEi | P12979 |
ProteomicsDBi | 286113 |
PTM databases
iPTMneti | P12979 |
PhosphoSitePlusi | P12979 |
Expressioni
Tissue specificityi
Expressed in myoblast cells. Expressed weakly in myotubes (at protein level). Expressed strongly in denervated muscles and in satellite cells isolated from denervated muscles. Expressed weakly in innervated muscle and in satellite cells isolated from innervated muscles.2 Publications
Developmental stagei
Expressed in the myotome of the somites at 8.5 dpc, onward (at protein level). Expressed in proximal region of both the hindlimb and the forelimb at 11.5 dpc, onward. Expressed during muscle maturation between 15 and 17 dpc and decreases thereafter. Not detected within the heart.3 Publications
Inductioni
Up-regulated in denervated muscles (at protein level). Up-regulated during myogenesis in the embryo and in cell culture models of myogenic differentiation via the p38 MAPK signaling pathway.3 Publications
Gene expression databases
Bgeei | ENSMUSG00000026459, Expressed in muscle of leg and 175 other tissues |
Genevisiblei | P12979, MM |
Interactioni
Subunit structurei
Homodimer and heterodimer with E12; heterodimerization enhances MYOG DNA-binding and transcriptional activities.
Interacts with SMARCA4/BRG1/BAF190A.
Interacts (via C-terminal region) with SSRP1 and SUPT16H; the interaction is indicative of an interaction with the FACT complex. nteracts with CSRP3 (By similarity).
By similarity3 PublicationsGO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 201674, 7 interactors |
CORUMi | P12979 |
DIPi | DIP-29976N |
IntActi | P12979, 5 interactors |
MINTi | P12979 |
STRINGi | 10090.ENSMUSP00000027730 |
Miscellaneous databases
RNActi | P12979, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 81 – 132 | bHLHPROSITE-ProRule annotationAdd BLAST | 52 |
Phylogenomic databases
eggNOGi | KOG3960, Eukaryota |
GeneTreei | ENSGT00950000182959 |
HOGENOMi | CLU_100258_0_0_1 |
InParanoidi | P12979 |
OMAi | YFFPEQR |
OrthoDBi | 1471470at2759 |
PhylomeDBi | P12979 |
TreeFami | TF316344 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR002546, MyoD_N IPR039704, Myogenic_factor |
PANTHERi | PTHR11534, PTHR11534, 1 hit |
Pfami | View protein in Pfam PF01586, Basic, 1 hit PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00520, BASIC, 1 hit SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
P12979-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELYETSPYF YQEPHFYDGE NYLPVHLQGF EPPGYERTEL SLSPEARGPL
60 70 80 90 100
EEKGLGTPEH CPGQCLPWAC KVCKRKSVSV DRRRAATLRE KRRLKKVNEA
110 120 130 140 150
FEALKRSTLL NPNQRLPKVE ILRSAIQYIE RLQALLSSLN QEERDLRYRG
160 170 180 190 200
GGGPQPMVPS ECNSHSASCS PEWGNALEFG PNPGDHLLAA DPTDAHNLHS
210 220
LTSIVDSITV EDMSVAFPDE TMPN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95800 Genomic DNA Translation: AAB59676.1 X15784 mRNA Translation: CAA33785.1 Sequence problems. D90156 mRNA Translation: BAA14187.1 BC048683 mRNA Translation: AAH48683.1 BC068019 mRNA Translation: AAH68019.1 |
CCDSi | CCDS15306.1 |
PIRi | A35882, A36675 |
RefSeqi | NP_112466.1, NM_031189.2 |
Genome annotation databases
Ensembli | ENSMUST00000027730; ENSMUSP00000027730; ENSMUSG00000026459 |
GeneIDi | 17928 |
KEGGi | mmu:17928 |
UCSCi | uc007crl.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95800 Genomic DNA Translation: AAB59676.1 X15784 mRNA Translation: CAA33785.1 Sequence problems. D90156 mRNA Translation: BAA14187.1 BC048683 mRNA Translation: AAH48683.1 BC068019 mRNA Translation: AAH68019.1 |
CCDSi | CCDS15306.1 |
PIRi | A35882, A36675 |
RefSeqi | NP_112466.1, NM_031189.2 |
3D structure databases
SMRi | P12979 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201674, 7 interactors |
CORUMi | P12979 |
DIPi | DIP-29976N |
IntActi | P12979, 5 interactors |
MINTi | P12979 |
STRINGi | 10090.ENSMUSP00000027730 |
PTM databases
iPTMneti | P12979 |
PhosphoSitePlusi | P12979 |
Proteomic databases
EPDi | P12979 |
PaxDbi | P12979 |
PRIDEi | P12979 |
ProteomicsDBi | 286113 |
Protocols and materials databases
ABCDi | P12979, 1 sequenced antibody |
Antibodypediai | 20659, 1073 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000027730; ENSMUSP00000027730; ENSMUSG00000026459 |
GeneIDi | 17928 |
KEGGi | mmu:17928 |
UCSCi | uc007crl.2, mouse |
Organism-specific databases
CTDi | 4656 |
MGIi | MGI:97276, Myog |
Phylogenomic databases
eggNOGi | KOG3960, Eukaryota |
GeneTreei | ENSGT00950000182959 |
HOGENOMi | CLU_100258_0_0_1 |
InParanoidi | P12979 |
OMAi | YFFPEQR |
OrthoDBi | 1471470at2759 |
PhylomeDBi | P12979 |
TreeFami | TF316344 |
Enzyme and pathway databases
Reactomei | R-MMU-525793, Myogenesis |
Miscellaneous databases
BioGRID-ORCSi | 17928, 0 hits in 52 CRISPR screens |
PROi | PR:P12979 |
RNActi | P12979, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026459, Expressed in muscle of leg and 175 other tissues |
Genevisiblei | P12979, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR002546, MyoD_N IPR039704, Myogenic_factor |
PANTHERi | PTHR11534, PTHR11534, 1 hit |
Pfami | View protein in Pfam PF01586, Basic, 1 hit PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00520, BASIC, 1 hit SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MYOG_MOUSE | |
Accessioni | P12979Primary (citable) accession number: P12979 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1990 |
Last sequence update: | December 1, 1992 | |
Last modified: | April 7, 2021 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot