Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neuroendocrine protein 7B2

Gene

Scg5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro.2 Publications

GO - Molecular functioni

  • enzyme inhibitor activity Source: UniProtKB
  • unfolded protein binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionChaperone, Neuropeptide
Biological processTransport

Protein family/group databases

MEROPSiI21.001

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroendocrine protein 7B2
Alternative name(s):
Secretogranin V
Secretogranin-5
Secretory granule endocrine protein I
Cleaved into the following 2 chains:
Gene namesi
Name:Scg5
Synonyms:Sgne-1, Sgne1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:98289 Scg5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Mice have no demonstrable Pcsk2/Pc2 activity, are deficient in processing islet hormones, and display hypoglycemia, hyperproinsulinemia and hypoglucagonemia, similar to Pcsk2 null mice. In contrast to Pcsk2 null mice, they develop Cushing's disease due to excessive secretion of corticotropin from the pituitary and die before 9 weeks, indicating a role for Sgne1 in control of peptide secretion from the pituitary.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi177R → A: No effect on proteolytic processing. Abolishes proteolytic processing; when associated with G-178. 1 Publication1
Mutagenesisi178R → G: Abolishes proteolytic processing; when associated with A-177. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Add BLAST26
ChainiPRO_000000004427 – 212Neuroendocrine protein 7B2Add BLAST186
ChainiPRO_000000004527 – 176N-terminal peptideBy similarityAdd BLAST150
PeptideiPRO_0000000046200 – 212C-terminal peptideBy similarityAdd BLAST13

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi120 ↔ 130By similarity
Modified residuei141PhosphoserineCombined sources1
Modified residuei205PhosphoserineCombined sources1

Post-translational modificationi

Proteolytically cleaved in the Golgi by a furin-like convertase to generate bioactive peptides.1 Publication
Sulfated on tyrosine residues.1 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Phosphoprotein, Sulfation

Proteomic databases

PaxDbiP12961
PeptideAtlasiP12961
PRIDEiP12961

PTM databases

iPTMnetiP12961
PhosphoSitePlusiP12961

Expressioni

Gene expression databases

BgeeiENSMUSG00000023236 Expressed in 207 organ(s), highest expression level in islet of Langerhans
CleanExiMM_SCG5
ExpressionAtlasiP12961 baseline and differential
GenevisibleiP12961 MM

Interactioni

Subunit structurei

Interacts with PCSK2/PC2 early in the secretory pathway. Dissociation occurs at later stages (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP12961, 1 interactor
MINTiP12961
STRINGi10090.ENSMUSP00000024005

Structurei

3D structure databases

ProteinModelPortaliP12961
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 7B2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4187 Eukaryota
ENOG410ZRXV LUCA
GeneTreeiENSGT00390000009816
HOGENOMiHOG000259690
HOVERGENiHBG000031
InParanoidiP12961
OMAiVGPQSIQ
OrthoDBiEOG091G0LE0
PhylomeDBiP12961
TreeFamiTF314328

Family and domain databases

InterProiView protein in InterPro
IPR007945 Secretogranin_V
PANTHERiPTHR12738 PTHR12738, 1 hit
PfamiView protein in Pfam
PF05281 Secretogranin_V, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12961-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASRLVSAML SGLLFWLMFE WNPAFAYSPR TPDRVSETDI QRLLHGVMEQ
60 70 80 90 100
LGIARPRVEY PAHQAMNLVG PQSIEGGAHE GLQHLGPFGN IPNIVAELTG
110 120 130 140 150
DNIPKDFSED QGYPDPPNPC PLGKTADDGC LENAPDTAEF SREFQLDQHL
160 170 180 190 200
FDPEHDYPGL GKWNKKLLYE KMKGGQRRKR RSVNPYLQGK RLDNVVAKKS
210
VPHFSEEEKE AE
Length:212
Mass (Da):23,866
Last modified:January 1, 1990 - v1
Checksum:i6D8CD046532F9609
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6V → G in BAB23956 (PubMed:16141072).Curated1
Sequence conflicti119P → H in BAB30765 (PubMed:16141072).Curated1
Sequence conflicti145Q → R in BAB31669 (PubMed:16141072).Curated1
Sequence conflicti153P → T in BAC27581 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15830 mRNA Translation: CAA33835.1
AK005331 mRNA Translation: BAB23956.1
AK011938 mRNA Translation: BAB27927.1
AK017481 mRNA Translation: BAB30765.1
AK019337 mRNA Translation: BAB31669.1
AK031856 mRNA Translation: BAC27581.1
BC029021 mRNA Translation: AAH29021.1
CCDSiCCDS16561.1
PIRiS12477
RefSeqiNP_033188.3, NM_009162.3
UniGeneiMm.4836

Genome annotation databases

EnsembliENSMUST00000024005; ENSMUSP00000024005; ENSMUSG00000023236
GeneIDi20394
KEGGimmu:20394
UCSCiuc008lpq.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15830 mRNA Translation: CAA33835.1
AK005331 mRNA Translation: BAB23956.1
AK011938 mRNA Translation: BAB27927.1
AK017481 mRNA Translation: BAB30765.1
AK019337 mRNA Translation: BAB31669.1
AK031856 mRNA Translation: BAC27581.1
BC029021 mRNA Translation: AAH29021.1
CCDSiCCDS16561.1
PIRiS12477
RefSeqiNP_033188.3, NM_009162.3
UniGeneiMm.4836

3D structure databases

ProteinModelPortaliP12961
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP12961, 1 interactor
MINTiP12961
STRINGi10090.ENSMUSP00000024005

Protein family/group databases

MEROPSiI21.001

PTM databases

iPTMnetiP12961
PhosphoSitePlusiP12961

Proteomic databases

PaxDbiP12961
PeptideAtlasiP12961
PRIDEiP12961

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024005; ENSMUSP00000024005; ENSMUSG00000023236
GeneIDi20394
KEGGimmu:20394
UCSCiuc008lpq.1 mouse

Organism-specific databases

CTDi6447
MGIiMGI:98289 Scg5

Phylogenomic databases

eggNOGiKOG4187 Eukaryota
ENOG410ZRXV LUCA
GeneTreeiENSGT00390000009816
HOGENOMiHOG000259690
HOVERGENiHBG000031
InParanoidiP12961
OMAiVGPQSIQ
OrthoDBiEOG091G0LE0
PhylomeDBiP12961
TreeFamiTF314328

Miscellaneous databases

PROiPR:P12961
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023236 Expressed in 207 organ(s), highest expression level in islet of Langerhans
CleanExiMM_SCG5
ExpressionAtlasiP12961 baseline and differential
GenevisibleiP12961 MM

Family and domain databases

InterProiView protein in InterPro
IPR007945 Secretogranin_V
PANTHERiPTHR12738 PTHR12738, 1 hit
PfamiView protein in Pfam
PF05281 Secretogranin_V, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei7B2_MOUSE
AccessioniPrimary (citable) accession number: P12961
Secondary accession number(s): Q8CCZ3
, Q9CYP0, Q9D2P6, Q9DB14
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: September 12, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again