UniProtKB - P12870 (CAPSD_FHV)
Capsid protein alpha
alpha
Functioni
Capsid protein alpha self-assembles to form an icosahedral procapsid with a T=3 symmetry, about 30 nm in diameter, and consisting of 60 capsid proteins trimers. In addition, 240 calcium ions are incorporated per capsid during assembly. The capsid encapsulates the two genomic RNAs. Capsid maturation occurs via autoproteolytic cleavage of capsid protein alpha generating capsid protein beta and the membrane-active peptide gamma.
Membrane-permeabilizing peptide produced by virus maturation, thereby creating the infectious virion. After endocytosis into the host cell, peptide gamma is probably exposed in endosomes, where it permeabilizes the endosomal membrane, facilitating translocation of viral capsid or RNA into the cytoplasm (Probable).
CuratedCatalytic activityi
- Hydrolysis of an asparaginyl bond involved in the maturation of the structural protein of the virus, typically -Asn-|-Ala- or -Asn-|-Phe-. EC:3.4.23.44
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 75 | By similarity | 1 | |
Metal bindingi | 249 | Calcium | 1 | |
Metal bindingi | 251 | Calcium | 1 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
- viral entry via permeabilization of inner membrane Source: UniProtKB-KW
Keywordsi
Molecular function | Aspartyl protease, Hydrolase, Protease |
Biological process | Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell |
Ligand | Calcium, Metal-binding |
Protein family/group databases
MEROPSi | N01.001 |
TCDBi | 1.A.61.1.2, the insect nodavirus channel-forming chain f (gamma-peptide) family |
Names & Taxonomyi
Protein namesi | Recommended name: Capsid protein alphaCleaved into the following 2 chains: Alternative name(s): Coat protein beta Nodavirus endopeptidase Alternative name(s): Coat protein gamma |
Gene namesi | Name:alpha |
Organismi | Flock house virus (FHV) |
Taxonomic identifieri | 12287 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Kitrinoviricota › Magsaviricetes › Nodamuvirales › Nodaviridae › Alphanodavirus |
Virus hosti | Costelytra zealandica [TaxID: 50579] Galleria mellonella (Greater wax moth) [TaxID: 7137] Hordeum vulgare (Barley) [TaxID: 4513] Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] |
Proteomesi |
|
Subcellular locationi
- Virion Curated
- Virion Curated Note: Located inside the capsid and probably externalized in early endosomes.Curated
Keywords - Cellular componenti
Capsid protein, T=3 icosahedral capsid protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000402388 | 1 – 407 | Capsid protein alphaAdd BLAST | 407 | |
ChainiPRO_0000039194 | 1 – 363 | Capsid protein betaAdd BLAST | 363 | |
ChainiPRO_0000039195 | 364 – 407 | Peptide gammaAdd BLAST | 44 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 69 ↔ 318 |
Post-translational modificationi
Keywords - PTMi
Disulfide bondStructurei
Secondary structure
3D structure databases
SMRi | P12870 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P12870 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 47 | DisorderedSequence analysisAdd BLAST | 47 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 29 | Polar residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 33 – 47 | Basic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Family and domain databases
Gene3Di | 2.60.120.20, 1 hit |
InterProi | View protein in InterPro IPR000696, Peptidase_A6 IPR029053, Viral_coat |
Pfami | View protein in Pfam PF01829, Peptidase_A6, 1 hit |
PRINTSi | PR00863, NODAVIRPTASE |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MVNNNRPRRQ RAQRVVVTTT QTAPVPQQNV PRNGRRRRNR TRRNRRRVRG
60 70 80 90 100
MNMAALTRLS QPGLAFLKCA FAPPDFNTDP GKGIPDRFEG KVVSRKDVLN
110 120 130 140 150
QSISFTAGQD TFILIAPTPG VAYWSASVPA GTFPTSATTF NPVNYPGFTS
160 170 180 190 200
MFGTTSTSRS DQVSSFRYAS MNVGIYPTSN LMQFAGSITV WKCPVKLSTV
210 220 230 240 250
QFPVATDPAT SSLVHTLVGL DGVLAVGPDN FSESFIKGVF SQSACNEPDF
260 270 280 290 300
EFNDILEGIQ TLPPANVSLG STGQPFTMDS GAEATSGVVG WGNMDTIVIR
310 320 330 340 350
VSAPEGAVNS AILKAWSCIE YRPNPNAMLY QFGHDSPPLD EVALQEYRTV
360 370 380 390 400
ARSLPVAVIA AQNASMWERV KSIIKSSLAA ASNIPGPIGV AASGISGLSA
LFEGFGF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15959 Genomic RNA Translation: CAA34081.1 |
PIRi | B34011, VCBBFH |
RefSeqi | NP_689442.1, NC_004144.1 |
Genome annotation databases
GeneIDi | 962115 |
KEGGi | vg:962115 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Virus Particle ExploreR db Icosahedral capsid structure |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15959 Genomic RNA Translation: CAA34081.1 |
PIRi | B34011, VCBBFH |
RefSeqi | NP_689442.1, NC_004144.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3LOB | X-ray | 3.60 | A/B/C | 1-363 | [»] | |
D/E/F | 364-407 | [»] | ||||
4FSJ | X-ray | 3.50 | A/B/C | 1-363 | [»] | |
D/E/F | 364-407 | [»] | ||||
4FTB | X-ray | 2.70 | A/B/C | 1-363 | [»] | |
D/E/F | 364-407 | [»] | ||||
4FTE | X-ray | 3.50 | A/B/C | 1-407 | [»] | |
4FTS | X-ray | 3.20 | A/B/C | 1-407 | [»] | |
6ITB | electron microscopy | 4.70 | A/B/C | 1-363 | [»] | |
6ITF | electron microscopy | 4.70 | A/B/C | 1-363 | [»] | |
SMRi | P12870 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
MEROPSi | N01.001 |
TCDBi | 1.A.61.1.2, the insect nodavirus channel-forming chain f (gamma-peptide) family |
Genome annotation databases
GeneIDi | 962115 |
KEGGi | vg:962115 |
Miscellaneous databases
EvolutionaryTracei | P12870 |
Family and domain databases
Gene3Di | 2.60.120.20, 1 hit |
InterProi | View protein in InterPro IPR000696, Peptidase_A6 IPR029053, Viral_coat |
Pfami | View protein in Pfam PF01829, Peptidase_A6, 1 hit |
PRINTSi | PR00863, NODAVIRPTASE |
MobiDBi | Search... |
Entry informationi
Entry namei | CAPSD_FHV | |
Accessioni | P12870Primary (citable) accession number: P12870 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | October 1, 1989 | |
Last modified: | February 23, 2022 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families