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Protein

cAMP-dependent protein kinase type I-beta regulatory subunit

Gene

Prkar1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei202cAMP 11
Binding sitei211cAMP 11
Binding sitei326cAMP 21
Binding sitei335cAMP 21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi137 – 254cAMP 1Add BLAST118
Nucleotide bindingi255 – 381cAMP 2Add BLAST127

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandcAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-163615 PKA activation
R-MMU-164378 PKA activation in glucagon signalling
R-MMU-180024 DARPP-32 events
R-MMU-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-MMU-5610787 Hedgehog 'off' state
R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type I-beta regulatory subunit
Gene namesi
Name:Prkar1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:97759 Prkar1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002053821 – 381cAMP-dependent protein kinase type I-beta regulatory subunitAdd BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3PhosphoserineBy similarity1
Disulfide bondi18Interchain (with C-39)By similarity
Modified residuei21Nitrated tyrosineBy similarity1
Disulfide bondi39Interchain (with C-18)By similarity
Modified residuei77PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei85PhosphothreonineCombined sources1
Modified residuei97Omega-N-methylarginineCombined sources1

Post-translational modificationi

The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.

Keywords - PTMi

Disulfide bond, Methylation, Nitration, Phosphoprotein

Proteomic databases

MaxQBiP12849
PaxDbiP12849
PeptideAtlasiP12849
PRIDEiP12849

PTM databases

iPTMnetiP12849
PhosphoSitePlusiP12849

Expressioni

Tissue specificityi

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Gene expression databases

BgeeiENSMUSG00000025855 Expressed in 301 organ(s), highest expression level in substantia nigra
ExpressionAtlasiP12849 baseline and differential
GenevisibleiP12849 MM

Interactioni

Subunit structurei

The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase (By similarity). Interacts with smAKAP; this interaction may target PRKAR1B to the plasma membrane (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202366, 5 interactors
CORUMiP12849
IntActiP12849, 7 interactors
STRINGi10090.ENSMUSP00000026973

Structurei

3D structure databases

ProteinModelPortaliP12849
SMRiP12849
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 136Dimerization and phosphorylationAdd BLAST136

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi96 – 100Pseudophosphorylation motif5

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1113 Eukaryota
COG0664 LUCA
GeneTreeiENSGT00530000062947
HOGENOMiHOG000196669
HOVERGENiHBG002025
InParanoidiP12849
KOiK04739
OMAiAMFPCNF
OrthoDBiEOG091G0F1K
PhylomeDBiP12849
TreeFamiTF314920

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR012198 cAMP_dep_PK_reg_su
IPR003117 cAMP_dep_PK_reg_su_I/II_a/b
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF02197 RIIa, 1 hit
PIRSFiPIRSF000548 PK_regulatory, 1 hit
SMARTiView protein in SMART
SM00100 cNMP, 2 hits
SM00394 RIIa, 1 hit
SUPFAMiSSF51206 SSF51206, 2 hits
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 2 hits
PS00889 CNMP_BINDING_2, 2 hits
PS50042 CNMP_BINDING_3, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All

P12849-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASPSCFHSE DEDSLKGCEM YVQKHGIQQV LKECIVHLCV AKPDRPLRFL
60 70 80 90 100
REHFEKLEKE ENRQILARQK SNSQCDSHDE EISPTPPNPV VKARRRRGGV
110 120 130 140 150
SAEVYTEEDA VSYVRKVIPK DYKTMTALAK AISKNVLFSH LDDNERSDIF
160 170 180 190 200
DAMFPVTHIG GETVIQQGNE GDNFYVIDQG EVDVYVNGEW VTNISEGGSF
210 220 230 240 250
GELALIYGTP RAATVKAKTD LKLWGIDRDS YRRILMGSTL RKRKMYEEFL
260 270 280 290 300
SKVSILESLE KWERLTVADA LEPVQFEDGE KIVVQGEPGD DFYIITEGTA
310 320 330 340 350
SVLQRRSPNE EYVEVGRLGP SDYFGEIALL LNRPRAATVV ARGPLKCVKL
360 370 380
DRPRFERVLG PCSEILKRNI QRYNSFISLT V
Length:381
Mass (Da):43,224
Last modified:January 23, 2007 - v2
Checksum:i42287E553FA16023
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z0V6D3Z0V6_MOUSE
cAMP-dependent protein kinase type ...
Prkar1b
257Annotation score:
D3Z068D3Z068_MOUSE
cAMP-dependent protein kinase type ...
Prkar1b
251Annotation score:
D3YTM5D3YTM5_MOUSE
cAMP-dependent protein kinase type ...
Prkar1b
234Annotation score:
D3Z4L4D3Z4L4_MOUSE
cAMP-dependent protein kinase type ...
Prkar1b
45Annotation score:
D3Z406D3Z406_MOUSE
cAMP-dependent protein kinase type ...
Prkar1b
116Annotation score:
D3Z7E4D3Z7E4_MOUSE
cAMP-dependent protein kinase type ...
Prkar1b
85Annotation score:
D3Z4A5D3Z4A5_MOUSE
cAMP-dependent protein kinase type ...
Prkar1b
107Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20473 mRNA Translation: AAA39935.1
CCDSiCCDS19803.1
PIRiA30205 OKMSR1
RefSeqiNP_001240819.1, NM_001253890.1
NP_032949.3, NM_008923.3
XP_006504723.1, XM_006504660.3
XP_006504724.1, XM_006504661.3
XP_006504726.1, XM_006504663.2
XP_017176208.1, XM_017320719.1
UniGeneiMm.306163

Genome annotation databases

EnsembliENSMUST00000026973; ENSMUSP00000026973; ENSMUSG00000025855
ENSMUST00000110890; ENSMUSP00000106515; ENSMUSG00000025855
GeneIDi19085
KEGGimmu:19085
UCSCiuc009afy.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20473 mRNA Translation: AAA39935.1
CCDSiCCDS19803.1
PIRiA30205 OKMSR1
RefSeqiNP_001240819.1, NM_001253890.1
NP_032949.3, NM_008923.3
XP_006504723.1, XM_006504660.3
XP_006504724.1, XM_006504661.3
XP_006504726.1, XM_006504663.2
XP_017176208.1, XM_017320719.1
UniGeneiMm.306163

3D structure databases

ProteinModelPortaliP12849
SMRiP12849
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202366, 5 interactors
CORUMiP12849
IntActiP12849, 7 interactors
STRINGi10090.ENSMUSP00000026973

PTM databases

iPTMnetiP12849
PhosphoSitePlusiP12849

Proteomic databases

MaxQBiP12849
PaxDbiP12849
PeptideAtlasiP12849
PRIDEiP12849

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026973; ENSMUSP00000026973; ENSMUSG00000025855
ENSMUST00000110890; ENSMUSP00000106515; ENSMUSG00000025855
GeneIDi19085
KEGGimmu:19085
UCSCiuc009afy.2 mouse

Organism-specific databases

CTDi5575
MGIiMGI:97759 Prkar1b

Phylogenomic databases

eggNOGiKOG1113 Eukaryota
COG0664 LUCA
GeneTreeiENSGT00530000062947
HOGENOMiHOG000196669
HOVERGENiHBG002025
InParanoidiP12849
KOiK04739
OMAiAMFPCNF
OrthoDBiEOG091G0F1K
PhylomeDBiP12849
TreeFamiTF314920

Enzyme and pathway databases

ReactomeiR-MMU-163615 PKA activation
R-MMU-164378 PKA activation in glucagon signalling
R-MMU-180024 DARPP-32 events
R-MMU-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-MMU-5610787 Hedgehog 'off' state
R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

ChiTaRSiPrkar1b mouse
PROiPR:P12849
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025855 Expressed in 301 organ(s), highest expression level in substantia nigra
ExpressionAtlasiP12849 baseline and differential
GenevisibleiP12849 MM

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR012198 cAMP_dep_PK_reg_su
IPR003117 cAMP_dep_PK_reg_su_I/II_a/b
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF02197 RIIa, 1 hit
PIRSFiPIRSF000548 PK_regulatory, 1 hit
SMARTiView protein in SMART
SM00100 cNMP, 2 hits
SM00394 RIIa, 1 hit
SUPFAMiSSF51206 SSF51206, 2 hits
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 2 hits
PS00889 CNMP_BINDING_2, 2 hits
PS50042 CNMP_BINDING_3, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiKAP1_MOUSE
AccessioniPrimary (citable) accession number: P12849
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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