UniProtKB - P12813 (NR4A1_MOUSE)
Nuclear receptor subfamily 4 group A member 1
Nr4a1
Functioni
Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (By similarity).
Plays a role in the vascular response to injury (PubMed:22427340).
By similarity1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 267 – 342 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 76 | |
Zinc fingeri | 270 – 290 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 306 – 330 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- DNA binding Source: BHF-UCL
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- glucocorticoid receptor binding Source: MGI
- identical protein binding Source: MGI
- nuclear receptor activity Source: InterPro
- protein heterodimerization activity Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- apoptotic process Source: MGI
- cell migration involved in sprouting angiogenesis Source: MGI
- cellular response to corticotropin-releasing hormone stimulus Source: UniProtKB
- cellular response to fibroblast growth factor stimulus Source: MGI
- cellular response to organic substance Source: MGI
- cellular response to vascular endothelial growth factor stimulus Source: MGI
- endothelial cell chemotaxis Source: MGI
- fat cell differentiation Source: UniProtKB
- negative regulation of cell cycle Source: UniProtKB
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- neurotransmitter secretion involved in regulation of skeletal muscle contraction Source: MGI
- positive regulation of apoptotic process Source: MGI
- positive regulation of endothelial cell proliferation Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of type B pancreatic cell proliferation Source: UniProtKB
- response to amphetamine Source: MGI
- response to electrical stimulus Source: Ensembl
- response to inorganic substance Source: MGI
- response to lipopolysaccharide Source: Ensembl
- skeletal muscle cell differentiation Source: MGI
Keywordsi
Molecular function | DNA-binding, Receptor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-198693, AKT phosphorylates targets in the nucleus R-MMU-383280, Nuclear Receptor transcription pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear receptor subfamily 4 group A member 1Alternative name(s): Nuclear hormone receptor NUR/77 Nuclear protein N10 Orphan nuclear receptor HMR |
Gene namesi | Name:Nr4a1 Synonyms:Gfrp, Hmr, N10, Nur77 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1352454, Nr4a1 |
VEuPathDBi | HostDB:ENSMUSG00000023034 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Nucleus
- Nucleus 1 Publication
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: Nuclear export to the cytoplasm is XPO1-mediated and positively regulated by IFI27. Translocation to the mitochondrion upon interaction with RXRA and upon the presence of 9-cis retinoic acid (By similarity).By similarity
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: UniProtKB-SubCell
Nucleus
- nuclear membrane Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: BHF-UCL
Other locations
- presynapse Source: GOC
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, NucleusPathology & Biotechi
Disruption phenotypei
Chemistry databases
ChEMBLi | CHEMBL3308901 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053716 | 1 – 601 | Nuclear receptor subfamily 4 group A member 1Add BLAST | 601 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 344 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 354 | Phosphoserine; by PKA, RPS6KA1 and RPS6KA3By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | P12813 |
PRIDEi | P12813 |
ProteomicsDBi | 295525 |
PTM databases
iPTMneti | P12813 |
PhosphoSitePlusi | P12813 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000023034, Expressed in adrenal gland and 293 other tissues |
ExpressionAtlasi | P12813, baseline and differential |
Genevisiblei | P12813, MM |
Interactioni
Subunit structurei
Binds DNA as a monomer (By similarity).
Interacts with GADD45GIP1.
Interacts with STK11 (By similarity).
Interacts with IFI27 (By similarity). Heterodimer (via DNA-binding domain) with RXRA (via C-terminus); DNA-binding of the heterodimer is enhanced by 9-cis retinoic acid (By similarity). Competes for the RXRA interaction with EP300 and thereby attenuates EP300 mediated acetylation of RXRA (By similarity).
By similarityBinary interactionsi
P12813
GO - Molecular functioni
- glucocorticoid receptor binding Source: MGI
- identical protein binding Source: MGI
- protein heterodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 200346, 8 interactors |
CORUMi | P12813 |
DIPi | DIP-61643N |
ELMi | P12813 |
IntActi | P12813, 16 interactors |
STRINGi | 10090.ENSMUSP00000023779 |
Miscellaneous databases
RNActi | P12813, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 363 – 598 | NR LBDPROSITE-ProRule annotationAdd BLAST | 236 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 21 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 173 – 469 | Required for nuclear importBy similarityAdd BLAST | 297 | |
Regioni | 180 – 205 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 237 – 268 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 302 – 364 | Required for the interaction with RXRABy similarityAdd BLAST | 63 | |
Regioni | 345 – 364 | DisorderedSequence analysisAdd BLAST | 20 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 182 – 197 | Pro residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 239 – 263 | Polar residuesSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 270 – 290 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 306 – 330 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG4217, Eukaryota |
GeneTreei | ENSGT00950000183038 |
HOGENOMi | CLU_007368_14_2_1 |
InParanoidi | P12813 |
OMAi | MEVIRKW |
OrthoDBi | 454476at2759 |
PhylomeDBi | P12813 |
TreeFami | TF315430 |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR003071, Nuc_orp_HMR_rcpt IPR003070, Nuc_orph_rcpt IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR01285, HMRNUCRECPTR PR01284, NUCLEARECPTR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MPCIQAQYGT PATSPGPRDH LTGDPLALEF GKPTMDLASP ETAPAAPATL
60 70 80 90 100
PSFSTFMDGY TGEFDTFLYQ LPGTTQPCSS ACSSASSTSS SSSSATSPAS
110 120 130 140 150
ASFKFEDFQV YGCYPGTLSG PLDETLSSSG SEYYGSPCSA PSPSTPNFQP
160 170 180 190 200
SQLSPWDGSF GHFSPSQTYE GLWAWTEQLP KASSGPPPPP TFFSFSPPTG
210 220 230 240 250
PSPSLAQSSL KLFPPPATHQ LGEGESYSMP AAFPGLAPTS PNRDTSGILD
260 270 280 290 300
APVTSTKSRS GASGGSEGRC AVCGDNASCQ HYGVRTCEGC KGFFKRTVQK
310 320 330 340 350
SAKYICLANK DCPVDKRRRN RCQFCRFQKC LAVGMVKEVV RTDSLKGRRG
360 370 380 390 400
RLPSKPKQPP DASPTNLLTS LIRAHLDSGP STAKLDYSKF QELVLPRFGK
410 420 430 440 450
EDAGDVQQFY DLLSGSLDVI RKWAEKIPGF IELCPGDQDL LLESAFLELF
460 470 480 490 500
ILRLAYRSKP GEGKLIFCSG LVLHQLQCAR GFGDWIDNIL AFSRSLHSLG
510 520 530 540 550
VDVPAFACLS ALVLITDRHG LQDPRRVEEL QNRIASCLKE HMATVAGDPQ
560 570 580 590 600
PASCLSRLLG KLPELRTLCT QGLQRIFCLK LEDLVPPPPI VDKIFMDTLS
F
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A2R8VJZ1 | A0A2R8VJZ1_MOUSE | Nuclear receptor subfamily 4 group ... | Nr4a1 | 13 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 45 | A → T in CAA42718 (PubMed:2555161).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04113 mRNA Translation: AAA39843.1 X16995 Genomic DNA Translation: CAA34862.1 X60132 mRNA Translation: CAA42718.1 BC004770 mRNA Translation: AAH04770.1 |
CCDSi | CCDS27848.1 |
PIRi | S06953, QRMSN1 |
RefSeqi | NP_034574.1, NM_010444.2 XP_006520518.1, XM_006520455.1 XP_006520519.1, XM_006520456.1 XP_006520521.1, XM_006520458.1 XP_006520522.1, XM_006520459.3 XP_017171925.1, XM_017316436.1 |
Genome annotation databases
Ensembli | ENSMUST00000023779; ENSMUSP00000023779; ENSMUSG00000023034 ENSMUST00000228985; ENSMUSP00000155225; ENSMUSG00000023034 |
GeneIDi | 15370 |
KEGGi | mmu:15370 |
UCSCi | uc007xsv.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04113 mRNA Translation: AAA39843.1 X16995 Genomic DNA Translation: CAA34862.1 X60132 mRNA Translation: CAA42718.1 BC004770 mRNA Translation: AAH04770.1 |
CCDSi | CCDS27848.1 |
PIRi | S06953, QRMSN1 |
RefSeqi | NP_034574.1, NM_010444.2 XP_006520518.1, XM_006520455.1 XP_006520519.1, XM_006520456.1 XP_006520521.1, XM_006520458.1 XP_006520522.1, XM_006520459.3 XP_017171925.1, XM_017316436.1 |
3D structure databases
SMRi | P12813 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200346, 8 interactors |
CORUMi | P12813 |
DIPi | DIP-61643N |
ELMi | P12813 |
IntActi | P12813, 16 interactors |
STRINGi | 10090.ENSMUSP00000023779 |
Chemistry databases
ChEMBLi | CHEMBL3308901 |
PTM databases
iPTMneti | P12813 |
PhosphoSitePlusi | P12813 |
Proteomic databases
PaxDbi | P12813 |
PRIDEi | P12813 |
ProteomicsDBi | 295525 |
Protocols and materials databases
Antibodypediai | 14466, 719 antibodies from 45 providers |
DNASUi | 15370 |
Genome annotation databases
Ensembli | ENSMUST00000023779; ENSMUSP00000023779; ENSMUSG00000023034 ENSMUST00000228985; ENSMUSP00000155225; ENSMUSG00000023034 |
GeneIDi | 15370 |
KEGGi | mmu:15370 |
UCSCi | uc007xsv.2, mouse |
Organism-specific databases
CTDi | 3164 |
MGIi | MGI:1352454, Nr4a1 |
VEuPathDBi | HostDB:ENSMUSG00000023034 |
Phylogenomic databases
eggNOGi | KOG4217, Eukaryota |
GeneTreei | ENSGT00950000183038 |
HOGENOMi | CLU_007368_14_2_1 |
InParanoidi | P12813 |
OMAi | MEVIRKW |
OrthoDBi | 454476at2759 |
PhylomeDBi | P12813 |
TreeFami | TF315430 |
Enzyme and pathway databases
Reactomei | R-MMU-198693, AKT phosphorylates targets in the nucleus R-MMU-383280, Nuclear Receptor transcription pathway |
Miscellaneous databases
BioGRID-ORCSi | 15370, 4 hits in 64 CRISPR screens |
ChiTaRSi | Gchfr, mouse |
PROi | PR:P12813 |
RNActi | P12813, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000023034, Expressed in adrenal gland and 293 other tissues |
ExpressionAtlasi | P12813, baseline and differential |
Genevisiblei | P12813, MM |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR003071, Nuc_orp_HMR_rcpt IPR003070, Nuc_orph_rcpt IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR01285, HMRNUCRECPTR PR01284, NUCLEARECPTR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NR4A1_MOUSE | |
Accessioni | P12813Primary (citable) accession number: P12813 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | October 1, 1989 | |
Last modified: | February 23, 2022 | |
This is version 205 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families