UniProtKB - P12758 (UDP_ECOLI)
Protein
Uridine phosphorylase
Gene
udp
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.
Catalytic activityi
- EC:2.4.2.3
: UMP biosynthesis via salvage pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes uracil from uridine (phosphorylase route).Proteins known to be involved in this subpathway in this organism are:
- Uridine phosphorylase (FAZ83_14490), Uridine phosphorylase (udp)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes uracil from uridine (phosphorylase route), the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.
GO - Molecular functioni
- ATP binding Source: EcoCyc
- identical protein binding Source: EcoCyc
- potassium ion binding Source: EcoCyc
- uridine phosphorylase activity Source: EcoCyc
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
- nucleotide catabolic process Source: InterPro
- UMP salvage Source: UniProtKB-UniPathway
- uridine catabolic process Source: EcoCyc
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | EcoCyc:URPHOS-MONOMER MetaCyc:URPHOS-MONOMER |
BRENDAi | 2.4.2.3, 2026 |
UniPathwayi | UPA00574;UER00633 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:udp Ordered Locus Names:b3831, JW3808 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Other locations
- protein-containing complex Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 5 | D → A, E or N: No change in activity. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB07439, 1-((2-HYDROXYETHOXY)METHYL)-5-(3-(BENZYLOXY)BENZYL)-6-HYDROXYPYRIMIDINE-2,4(1H,3H)-DIONE DB06873, 1-((2-HYDROXYETHOXY)METHYL)-5-(3-(BENZYLOXY)BENZYL)PYRIMIDINE-2,4(1H,3H)-DIONE DB06872, 1-((2-HYDROXYETHOXY)METHYL)-5-(PHENYLTHIO)PYRIMIDINE-2,4(1H,3H)-DIONE DB02256, 2'-Deoxyuridine DB02681, 2,8-bis[oxido(oxo)vanadio]-1,1,1,3,5,5,7,7,9,9,9-undecaoxopentavanadoxane-2,8-diium DB07437, 5-Benzylacyclouridine DB01629, 5-fluorouridine DB03101, Ribose-1-Phosphate DB04485, Thymidine |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed3 Publications | |||
ChainiPRO_0000063183 | 2 – 253 | Uridine phosphorylaseAdd BLAST | 252 |
Proteomic databases
jPOSTi | P12758 |
PaxDbi | P12758 |
PRIDEi | P12758 |
2D gel databases
SWISS-2DPAGEi | P12758 |
Interactioni
Subunit structurei
Homohexamer. The homohexamer shows 4-fold, 6-fold or 8-fold symmetry.
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4263299, 11 interactors |
DIPi | DIP-11075N |
IntActi | P12758, 4 interactors |
MINTi | P12758 |
STRINGi | 511145.b3831 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P12758 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P12758 |
Family & Domainsi
Sequence similaritiesi
Belongs to the PNP/UDP phosphorylase family.Curated
Phylogenomic databases
eggNOGi | COG2820, Bacteria |
HOGENOMi | CLU_068457_0_0_6 |
InParanoidi | P12758 |
PhylomeDBi | P12758 |
Family and domain databases
Gene3Di | 3.40.50.1580, 1 hit |
InterProi | View protein in InterPro IPR018016, Nucleoside_phosphorylase_CS IPR000845, Nucleoside_phosphorylase_d IPR035994, Nucleoside_phosphorylase_sf IPR010058, Uridine_phosphorylase |
Pfami | View protein in Pfam PF01048, PNP_UDP_1, 1 hit |
SUPFAMi | SSF53167, SSF53167, 1 hit |
TIGRFAMsi | TIGR01718, Uridine-psphlse, 1 hit |
PROSITEi | View protein in PROSITE PS01232, PNP_UDP_1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P12758-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKSDVFHLG LTKNDLQGAT LAIVPGDPDR VEKIAALMDK PVKLASHREF
60 70 80 90 100
TTWRAELDGK PVIVCSTGIG GPSTSIAVEE LAQLGIRTFL RIGTTGAIQP
110 120 130 140 150
HINVGDVLVT TASVRLDGAS LHFAPLEFPA VADFECTTAL VEAAKSIGAT
160 170 180 190 200
THVGVTASSD TFYPGQERYD TYSGRVVRHF KGSMEEWQAM GVMNYEMESA
210 220 230 240 250
TLLTMCASQG LRAGMVAGVI VNRTQQEIPN AETMKQTESH AVKIVVEAAR
RLL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 11 | L → Y AA sequence (PubMed:8899705).Curated | 1 | |
Sequence conflicti | 15 | D → Y AA sequence (PubMed:8899705).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15689 Genomic DNA Translation: CAA33724.1 M87049 Genomic DNA Translation: AAA67626.1 U00096 Genomic DNA Translation: AAC76834.1 AP009048 Genomic DNA Translation: BAE77470.1 |
PIRi | S05491 |
RefSeqi | NP_418275.1, NC_000913.3 WP_000045177.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76834; AAC76834; b3831 BAE77470; BAE77470; BAE77470 |
GeneIDi | 56808354 948987 |
KEGGi | ecj:JW3808 eco:b3831 |
PATRICi | fig|511145.12.peg.3947 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15689 Genomic DNA Translation: CAA33724.1 M87049 Genomic DNA Translation: AAA67626.1 U00096 Genomic DNA Translation: AAC76834.1 AP009048 Genomic DNA Translation: BAE77470.1 |
PIRi | S05491 |
RefSeqi | NP_418275.1, NC_000913.3 WP_000045177.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1K3F | X-ray | 2.50 | A/B/C/D/E/F | 1-253 | [»] | |
1LX7 | X-ray | 2.00 | A/B | 1-253 | [»] | |
1RXC | X-ray | 2.35 | A/B/C/D/E/F/G/H/I/J/K/L | 1-253 | [»] | |
1RXS | X-ray | 2.80 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/a/b/c/d/e/h/i/j/k/l/m/o | 1-253 | [»] | |
1RXU | X-ray | 3.10 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R | 1-253 | [»] | |
1RXY | X-ray | 1.70 | A/B | 1-253 | [»] | |
1T0U | X-ray | 2.20 | A/B | 1-253 | [»] | |
1TGV | X-ray | 2.20 | A/B | 1-253 | [»] | |
1TGY | X-ray | 2.20 | A/B | 1-253 | [»] | |
1U1C | X-ray | 2.20 | A/B/C/D/E/F | 2-253 | [»] | |
1U1D | X-ray | 2.00 | A/B/C/D/E/F | 2-253 | [»] | |
1U1E | X-ray | 2.00 | A/B/C/D/E/F | 2-253 | [»] | |
1U1F | X-ray | 2.30 | A/B/C/D/E/F | 2-253 | [»] | |
1U1G | X-ray | 1.95 | A/B/C/D/E/F | 2-253 | [»] | |
3KVV | X-ray | 1.80 | A/B/C/D/E/F | 1-253 | [»] | |
SMRi | P12758 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263299, 11 interactors |
DIPi | DIP-11075N |
IntActi | P12758, 4 interactors |
MINTi | P12758 |
STRINGi | 511145.b3831 |
Chemistry databases
DrugBanki | DB07439, 1-((2-HYDROXYETHOXY)METHYL)-5-(3-(BENZYLOXY)BENZYL)-6-HYDROXYPYRIMIDINE-2,4(1H,3H)-DIONE DB06873, 1-((2-HYDROXYETHOXY)METHYL)-5-(3-(BENZYLOXY)BENZYL)PYRIMIDINE-2,4(1H,3H)-DIONE DB06872, 1-((2-HYDROXYETHOXY)METHYL)-5-(PHENYLTHIO)PYRIMIDINE-2,4(1H,3H)-DIONE DB02256, 2'-Deoxyuridine DB02681, 2,8-bis[oxido(oxo)vanadio]-1,1,1,3,5,5,7,7,9,9,9-undecaoxopentavanadoxane-2,8-diium DB07437, 5-Benzylacyclouridine DB01629, 5-fluorouridine DB03101, Ribose-1-Phosphate DB04485, Thymidine |
2D gel databases
SWISS-2DPAGEi | P12758 |
Proteomic databases
jPOSTi | P12758 |
PaxDbi | P12758 |
PRIDEi | P12758 |
Genome annotation databases
EnsemblBacteriai | AAC76834; AAC76834; b3831 BAE77470; BAE77470; BAE77470 |
GeneIDi | 56808354 948987 |
KEGGi | ecj:JW3808 eco:b3831 |
PATRICi | fig|511145.12.peg.3947 |
Organism-specific databases
EchoBASEi | EB1038 |
Phylogenomic databases
eggNOGi | COG2820, Bacteria |
HOGENOMi | CLU_068457_0_0_6 |
InParanoidi | P12758 |
PhylomeDBi | P12758 |
Enzyme and pathway databases
UniPathwayi | UPA00574;UER00633 |
BioCyci | EcoCyc:URPHOS-MONOMER MetaCyc:URPHOS-MONOMER |
BRENDAi | 2.4.2.3, 2026 |
Miscellaneous databases
EvolutionaryTracei | P12758 |
PROi | PR:P12758 |
Family and domain databases
Gene3Di | 3.40.50.1580, 1 hit |
InterProi | View protein in InterPro IPR018016, Nucleoside_phosphorylase_CS IPR000845, Nucleoside_phosphorylase_d IPR035994, Nucleoside_phosphorylase_sf IPR010058, Uridine_phosphorylase |
Pfami | View protein in Pfam PF01048, PNP_UDP_1, 1 hit |
SUPFAMi | SSF53167, SSF53167, 1 hit |
TIGRFAMsi | TIGR01718, Uridine-psphlse, 1 hit |
PROSITEi | View protein in PROSITE PS01232, PNP_UDP_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UDP_ECOLI | |
Accessioni | P12758Primary (citable) accession number: P12758 Secondary accession number(s): Q2M8D6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 187 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families