UniProtKB - P12758 (UDP_ECOLI)

>sp|P12758|UDP_ECOLI Uridine phosphorylase OS=Escherichia coli (strain K12) OX=83333 GN=udp PE=1 SV=3
MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKLASHREFTTWRAELDGK
PVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHINVGDVLVTTASVRLDGAS
LHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSDTFYPGQERYDTYSGRVVRHF
KGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAGVIVNRTQQEIPNAETMKQTESH
AVKIVVEAARRLL

Entry version 177 (08 May 2019)
Sequence version 3 (23 Jan 2007)
Previous versions | rss

Other tutorials and videos Help videoFeedback

Protein

Uridine phosphorylase

Gene

udp

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.

Catalytic activityⁱ

phosphate
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
uridine
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
=
α-D-ribose 1-phosphate
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
uracil
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
EC:
2.4.2.3
- Search proteins in UniProtKB for this EC number.
- See the description of this EC number in ENZYME.
Source:
Rhea
Search for this reaction in UniProtKB.
See the description of this reaction in Rhea.
. Show »« Hide
phosphate
zoom
+
uridine
zoom
=
α-D-ribose 1-phosphate
zoom
+
uracil
zoom

Pathwayⁱ: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes uracil from uridine (phosphorylase route).
Proteins known to be involved in this subpathway in this organism are:

Uridine phosphorylase (udp)

This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes uracil from uridine (phosphorylase route), the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosyltransferase, Transferase

Molecular function

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCycⁱ	EcoCyc:URPHOS-MONOMER ECOL316407:JW3808-MONOMER MetaCyc:URPHOS-MONOMER
BRENDAⁱ	2.4.2.3 2026
UniPathwayⁱ	UPA00574;UER00633

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Uridine phosphorylase (EC:2.4.2.3) Short name: UPase Short name: UrdPase
Gene namesⁱ	Name:udp Ordered Locus Names:b3831, JW3808
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11045 udp

EcoGeneⁱ

EG11045 udp

Subcellular locationⁱ

Cytoplasm

GO - Cellular componentⁱ

Complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	5	D → A, E or N: No change in activity. 1 Publication		1

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB02256 2'-Deoxyuridine DB01629 5-Fluorouridine DB04485 Deoxythymidine DB02681 Meta Vanadate DB03101 Ribose-1-Phosphate

DrugBankⁱ

DB02256 2'-Deoxyuridine
DB01629 5-Fluorouridine
DB04485 Deoxythymidine
DB02681 Meta Vanadate
DB03101 Ribose-1-Phosphate

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		Removed3 Publications
ChainⁱPRO_0000063183	2 – 253	Uridine phosphorylaseAdd BLAST		252

Proteomic databases

EPDⁱ	P12758
jPOSTⁱ	P12758
PaxDbⁱ	P12758
PRIDEⁱ	P12758

2D gel databases

SWISS-2DPAGEⁱ	P12758

SWISS-2DPAGEⁱ

P12758

Interactionⁱ

Subunit structureⁱ

Homohexamer. The homohexamer shows 4-fold, 6-fold or 8-fold symmetry.

GO - Molecular functionⁱ

identical protein binding
Source: EcoCyc
Inferred from direct assayⁱ
- "Uridine phosphorylase from Escherichia coli. Physical and chemical characterization."
  Leer J.C., Hammer-Jespersen K., Schwartz M.
  Eur. J. Biochem. 75:217-224(1977) [PubMed] [Europe PMC] [Abstract]

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4263299, 11 interactors
Database of interacting proteins More...DIPⁱ	DIP-11075N
IntActⁱ	P12758, 4 interactors
Molecular INTeraction database More...MINTⁱ	P12758
STRINGⁱ	511145.b3831

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	6 – 9	Combined sources	4
Helixⁱ	13 – 16	Combined sources	4
Beta strandⁱ	21 – 23	Combined sources	3
Helixⁱ	28 – 30	Combined sources	3
Helixⁱ	31 – 35	Combined sources	5
Beta strandⁱ	38 – 47	Combined sources	10
Beta strandⁱ	50 – 57	Combined sources	8
Beta strandⁱ	60 – 65	Combined sources	6
Helixⁱ	71 – 83	Combined sources	13
Beta strandⁱ	88 – 97	Combined sources	10
Beta strandⁱ	107 – 116	Combined sources	10
Helixⁱ	119 – 122	Combined sources	4
Helixⁱ	134 – 146	Combined sources	13
Beta strandⁱ	151 – 160	Combined sources	10
Helixⁱ	164 – 166	Combined sources	3
Beta strandⁱ	171 – 173	Combined sources	3
Helixⁱ	178 – 180	Combined sources	3
Helixⁱ	183 – 189	Combined sources	7
Beta strandⁱ	194 – 198	Combined sources	5
Helixⁱ	199 – 207	Combined sources	9
Turnⁱ	208 – 210	Combined sources	3
Beta strandⁱ	212 – 222	Combined sources	11
Turnⁱ	223 – 225	Combined sources	3
Helixⁱ	231 – 251	Combined sources	21

Show more details

3D structure databases

SMRⁱ	P12758
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P12758

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the PNP/UDP phosphorylase family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4108I5U Bacteria COG2820 LUCA
HOGENOMⁱ	HOG000274897
InParanoidⁱ	P12758
KEGG Orthology (KO) More...KOⁱ	K00757
PhylomeDBⁱ	P12758

Family and domain databases

Gene3Dⁱ	3.40.50.1580, 1 hit
InterProⁱ	View protein in InterPro IPR018016 Nucleoside_phosphorylase_CS IPR000845 Nucleoside_phosphorylase_d IPR035994 Nucleoside_phosphorylase_sf IPR010058 Uridine_phosphorylase
PANTHERⁱ	PTHR43691:SF7 PTHR43691:SF7, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01048 PNP_UDP_1, 1 hit
SUPFAMⁱ	SSF53167 SSF53167, 1 hit
TIGRFAMsⁱ	TIGR01718 Uridine-psphlse, 1 hit
PROSITEⁱ	View protein in PROSITE PS01232 PNP_UDP_1, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P12758-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSKSDVFHLG LTKNDLQGAT LAIVPGDPDR VEKIAALMDK PVKLASHREF 
        60         70         80         90        100
TTWRAELDGK PVIVCSTGIG GPSTSIAVEE LAQLGIRTFL RIGTTGAIQP 
       110        120        130        140        150
HINVGDVLVT TASVRLDGAS LHFAPLEFPA VADFECTTAL VEAAKSIGAT 
       160        170        180        190        200
THVGVTASSD TFYPGQERYD TYSGRVVRHF KGSMEEWQAM GVMNYEMESA 
       210        220        230        240        250
TLLTMCASQG LRAGMVAGVI VNRTQQEIPN AETMKQTESH AVKIVVEAAR 

RLL

Length:253

Mass (Da):27,159

Last modified:January 23, 2007 - v3

Checksum:ⁱ7446DE45BA04D88D

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	11	L → Y AA sequence (PubMed:8899705).Curated		1
Sequence conflictⁱ	15	D → Y AA sequence (PubMed:8899705).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X15689 Genomic DNA Translation: CAA33724.1 M87049 Genomic DNA Translation: AAA67626.1 U00096 Genomic DNA Translation: AAC76834.1 AP009048 Genomic DNA Translation: BAE77470.1
PIRⁱ	S05491
NCBI Reference Sequences More...RefSeqⁱ	NP_418275.1, NC_000913.3 WP_000045177.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC76834; AAC76834; b3831 BAE77470; BAE77470; BAE77470
GeneIDⁱ	948987
KEGGⁱ	ecj:JW3808 eco:b3831
PATRICⁱ	fig\|511145.12.peg.3947

Protein	Similar proteins		Species	Score	Length	Source
P12758	Uridine phosphorylase		ECOLX		254	UniRef100_P12758
Uridine phosphorylase		ECO81		256
Uridine phosphorylase		Escherichia coli O25b:H4		256
Uridine phosphorylase		ECO7I		256
Uridine phosphorylase		Klebsiella pneumoniae IS22		254
+87

Protein	Similar proteins		Species	Score	Length	Source
P12758	Uridine phosphorylase		SALTI		253	UniRef90_P12758
Uridine phosphorylase	)	SALTY		253
Uridine phosphorylase		KLEAE		253
Uridine phosphorylase		ECOLX		254
Uridine phosphorylase		ECO81		256
+1090

Protein	Similar proteins		Species	Score	Length	Source
P12758	Uridine phosphorylase		SALTI		253	UniRef50_P12758
Uridine phosphorylase	)	SALTY		253
Uridine phosphorylase		KLEAE		253
Uridine phosphorylase		KLEPN		279
Uridine phosphorylase		HAEIN		252
+2702

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X15689 Genomic DNA Translation: CAA33724.1 M87049 Genomic DNA Translation: AAA67626.1 U00096 Genomic DNA Translation: AAC76834.1 AP009048 Genomic DNA Translation: BAE77470.1
PIRⁱ	S05491
RefSeqⁱ	NP_418275.1, NC_000913.3 WP_000045177.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1K3F	X-ray	2.50	A/B/C/D/E/F	1-253	[»]
	1LX7	X-ray	2.00	A/B	1-253	[»]
	1RXC	X-ray	2.35	A/B/C/D/E/F/G/H/I/J/K/L	1-253	[»]
	1RXS	X-ray	2.80	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/a/b/c/d/e/h/i/j/k/l/m/o	1-253	[»]
	1RXU	X-ray	3.10	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R	1-253	[»]
	1RXY	X-ray	1.70	A/B	1-253	[»]
	1T0U	X-ray	2.20	A/B	1-253	[»]
	1TGV	X-ray	2.20	A/B	1-253	[»]
	1TGY	X-ray	2.20	A/B	1-253	[»]
	1U1C	X-ray	2.20	A/B/C/D/E/F	2-253	[»]
	1U1D	X-ray	2.00	A/B/C/D/E/F	2-253	[»]
	1U1E	X-ray	2.00	A/B/C/D/E/F	2-253	[»]
	1U1F	X-ray	2.30	A/B/C/D/E/F	2-253	[»]
	1U1G	X-ray	1.95	A/B/C/D/E/F	2-253	[»]
	3KVV	X-ray	1.80	A/B/C/D/E/F	1-253	[»]
SMRⁱ	P12758
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4263299, 11 interactors
DIPⁱ	DIP-11075N
IntActⁱ	P12758, 4 interactors
MINTⁱ	P12758
STRINGⁱ	511145.b3831

Chemistry databases

DrugBankⁱ	DB02256 2'-Deoxyuridine DB01629 5-Fluorouridine DB04485 Deoxythymidine DB02681 Meta Vanadate DB03101 Ribose-1-Phosphate

DrugBankⁱ

DB02256 2'-Deoxyuridine
DB01629 5-Fluorouridine
DB04485 Deoxythymidine
DB02681 Meta Vanadate
DB03101 Ribose-1-Phosphate

2D gel databases

SWISS-2DPAGEⁱ	P12758

SWISS-2DPAGEⁱ

P12758

Proteomic databases

EPDⁱ	P12758
jPOSTⁱ	P12758
PaxDbⁱ	P12758
PRIDEⁱ	P12758

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76834; AAC76834; b3831 BAE77470; BAE77470; BAE77470
GeneIDⁱ	948987
KEGGⁱ	ecj:JW3808 eco:b3831
PATRICⁱ	fig\|511145.12.peg.3947

Organism-specific databases

EchoBASEⁱ	EB1038
EcoGeneⁱ	EG11045 udp

Phylogenomic databases

eggNOGⁱ	ENOG4108I5U Bacteria COG2820 LUCA
HOGENOMⁱ	HOG000274897
InParanoidⁱ	P12758
KOⁱ	K00757
PhylomeDBⁱ	P12758

Enzyme and pathway databases

UniPathwayⁱ	UPA00574;UER00633
BioCycⁱ	EcoCyc:URPHOS-MONOMER ECOL316407:JW3808-MONOMER MetaCyc:URPHOS-MONOMER
BRENDAⁱ	2.4.2.3 2026

Miscellaneous databases

EvolutionaryTraceⁱ	P12758
Protein Ontology More...PROⁱ	PR:P12758

Family and domain databases

Gene3Dⁱ	3.40.50.1580, 1 hit
InterProⁱ	View protein in InterPro IPR018016 Nucleoside_phosphorylase_CS IPR000845 Nucleoside_phosphorylase_d IPR035994 Nucleoside_phosphorylase_sf IPR010058 Uridine_phosphorylase
PANTHERⁱ	PTHR43691:SF7 PTHR43691:SF7, 1 hit
Pfamⁱ	View protein in Pfam PF01048 PNP_UDP_1, 1 hit
SUPFAMⁱ	SSF53167 SSF53167, 1 hit
TIGRFAMsⁱ	TIGR01718 Uridine-psphlse, 1 hit
PROSITEⁱ	View protein in PROSITE PS01232 PNP_UDP_1, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UDP_ECOLI
Accessionⁱ	P12758Primary (citable) accession number: P12758 Secondary accession number(s): Q2M8D6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
	Last sequence update:	January 23, 2007
	Last modified:	May 8, 2019
	This is version 177 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P12758 (UDP_ECOLI)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Uridine phosphorylase

udp

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: UMP biosynthesis via salvage pathway

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

2D gel databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

Catalytic activityⁱ

Pathwayⁱ: UMP biosynthesis via salvage pathway

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ