UniProtKB - P12724 (ECP_HUMAN)
Eosinophil cationic protein
RNASE3
Functioni
Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 42 | Proton acceptor | 1 | |
Sitei | 60 | May be involved in LPS-binding | 1 | |
Sitei | 62 | May be involved in LPS- and LTA-binding | 1 | |
Active sitei | 155 | Proton donor | 1 |
GO - Molecular functioni
- endonuclease activity Source: UniProtKB-KW
- lipopolysaccharide binding Source: UniProtKB
- nucleic acid binding Source: InterPro
- ribonuclease activity Source: GO_Central
GO - Biological processi
- antibacterial humoral response Source: UniProtKB
- antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
- chemotaxis Source: GO_Central
- defense response to Gram-negative bacterium Source: UniProtKB
- defense response to Gram-positive bacterium Source: UniProtKB
- induction of bacterial agglutination Source: UniProtKB
- innate immune response Source: UniProtKB
- innate immune response in mucosa Source: UniProtKB
- RNA catabolic process Source: ProtInc
Keywordsi
Molecular function | Antibiotic, Antimicrobial, Endonuclease, Hydrolase, Nuclease |
Enzyme and pathway databases
PathwayCommonsi | P12724 |
Reactomei | R-HSA-6798695, Neutrophil degranulation R-HSA-6803157, Antimicrobial peptides |
SignaLinki | P12724 |
SIGNORi | P12724 |
Names & Taxonomyi
Protein namesi | Recommended name: Eosinophil cationic protein (EC:3.1.27.-)Short name: ECP Alternative name(s): Ribonuclease 3 Short name: RNase 3 |
Gene namesi | Name:RNASE3 Synonyms:ECP, RNS3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10046, RNASE3 |
MIMi | 131398, gene |
neXtProti | NX_P12724 |
VEuPathDBi | HostDB:ENSG00000169397 |
Subcellular locationi
Extracellular region or secreted
Note: Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus.
Extracellular region or secreted
- extracellular region Source: Reactome
- extracellular space Source: UniProtKB
Lysosome
- azurophil granule lumen Source: Reactome
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 40 | I → A: Loss of in vitro formation of amyloid-like aggregates. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6037 |
OpenTargetsi | ENSG00000169397 |
PharmGKBi | PA34414 |
Miscellaneous databases
Pharosi | P12724, Tbio |
Chemistry databases
DrugBanki | DB02098, Adenosine-2'-5'-Diphosphate DB04272, Citric acid DB01411, Pranlukast |
Genetic variation databases
BioMutai | RNASE3 |
DMDMi | 147744558 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | 2 PublicationsAdd BLAST | 27 | |
ChainiPRO_0000030862 | 28 – 160 | Eosinophil cationic proteinAdd BLAST | 133 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 50 ↔ 110 | |||
Modified residuei | 60 | 3'-nitrotyrosine1 Publication | 1 | |
Disulfide bondi | 64 ↔ 123 | |||
Disulfide bondi | 82 ↔ 138 | |||
Glycosylationi | 84 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 89 ↔ 98 | |||
Glycosylationi | 92 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 119 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, NitrationProteomic databases
EPDi | P12724 |
jPOSTi | P12724 |
MassIVEi | P12724 |
PaxDbi | P12724 |
PeptideAtlasi | P12724 |
PRIDEi | P12724 |
ProteomicsDBi | 52864 |
PTM databases
GlyGeni | P12724, 3 sites |
iPTMneti | P12724 |
PhosphoSitePlusi | P12724 |
Expressioni
Gene expression databases
Bgeei | ENSG00000169397, Expressed in bone marrow and 113 other tissues |
Genevisiblei | P12724, HS |
Organism-specific databases
HPAi | ENSG00000169397, Tissue enriched (bone) |
Interactioni
Subunit structurei
Interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). In vitro interacts with and insert into lipid bilayers composed of dioleoyl phosphatidylcholine and dioleoyl phosphatidylglycerol. In vitro, tends to form amyloid-like aggregates at pH 3, but not at pH 5, nor 7.
1 PublicationProtein-protein interaction databases
BioGRIDi | 111966, 13 interactors |
IntActi | P12724, 13 interactors |
STRINGi | 9606.ENSP00000302324 |
Miscellaneous databases
RNActi | P12724, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P12724 |
SMRi | P12724 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P12724 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 28 – 72 | Required for nearly all of the bactericidal activity; partially involved in LPS-binding and bacterial membrane depolarizationAdd BLAST | 45 | |
Regioni | 65 – 69 | Substrate binding | 5 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502TF52, Eukaryota |
GeneTreei | ENSGT00940000162253 |
HOGENOMi | CLU_117006_0_1_1 |
InParanoidi | P12724 |
OMAi | NQSIRCP |
OrthoDBi | 1482425at2759 |
PhylomeDBi | P12724 |
TreeFami | TF333393 |
Family and domain databases
Gene3Di | 3.10.130.10, 1 hit |
InterProi | View protein in InterPro IPR001427, RNaseA IPR036816, RNaseA-like_dom_sf IPR023411, RNaseA_AS IPR023412, RNaseA_domain |
PANTHERi | PTHR11437, PTHR11437, 1 hit |
Pfami | View protein in Pfam PF00074, RnaseA, 1 hit |
PRINTSi | PR00794, RIBONUCLEASE |
SMARTi | View protein in SMART SM00092, RNAse_Pc, 1 hit |
SUPFAMi | SSF54076, SSF54076, 1 hit |
PROSITEi | View protein in PROSITE PS00127, RNASE_PANCREATIC, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MVPKLFTSQI CLLLLLGLMG VEGSLHARPP QFTRAQWFAI QHISLNPPRC
60 70 80 90 100
TIAMRAINNY RWRCKNQNTF LRTTFANVVN VCGNQSIRCP HNRTLNNCHR
110 120 130 140 150
SRFRVPLLHC DLINPGAQNI SNCTYADRPG RRFYVVACDN RDPRDSPRYP
160
VVPVHLDTTI
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_014109 | 72 | R → C1 PublicationCorresponds to variant dbSNP:rs151169198Ensembl. | 1 | |
Natural variantiVAR_013149 | 124 | T → R7 PublicationsCorresponds to variant dbSNP:rs2073342Ensembl. | 1 | |
Natural variantiVAR_029017 | 130 | G → R. Corresponds to variant dbSNP:rs12147890Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15161 mRNA Translation: CAA33251.1 M28128 mRNA Translation: AAA50283.1 X16545 Genomic DNA Translation: CAA34545.1 X55990 Genomic DNA Translation: CAA39462.1 AF294019 Genomic DNA Translation: AAG31589.1 AF294020 Genomic DNA Translation: AAG31590.1 AF294021 Genomic DNA Translation: AAG31591.1 AF294022 Genomic DNA Translation: AAG31592.1 AF294023 Genomic DNA Translation: AAG31593.1 AF294024 Genomic DNA Translation: AAG31594.1 AF294025 Genomic DNA Translation: AAG31595.1 AF294026 Genomic DNA Translation: AAG31596.1 AL133371 Genomic DNA No translation available. BC096060 mRNA Translation: AAH96060.1 BC096061 mRNA Translation: AAH96061.1 BC096062 mRNA Translation: AAH96062.1 AF441204 Genomic DNA Translation: AAL35279.1 AF441205 Genomic DNA Translation: AAL35280.1 AF441206 Genomic DNA Translation: AAL35281.1 |
CCDSi | CCDS9560.1 |
PIRi | B35328, JL0106 |
RefSeqi | NP_002926.2, NM_002935.2 |
Genome annotation databases
Ensembli | ENST00000304639; ENSP00000302324; ENSG00000169397 |
GeneIDi | 6037 |
KEGGi | hsa:6037 |
MANE-Selecti | ENST00000304639.4; ENSP00000302324.3; NM_002935.3; NP_002926.2 |
UCSCi | uc001vyj.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15161 mRNA Translation: CAA33251.1 M28128 mRNA Translation: AAA50283.1 X16545 Genomic DNA Translation: CAA34545.1 X55990 Genomic DNA Translation: CAA39462.1 AF294019 Genomic DNA Translation: AAG31589.1 AF294020 Genomic DNA Translation: AAG31590.1 AF294021 Genomic DNA Translation: AAG31591.1 AF294022 Genomic DNA Translation: AAG31592.1 AF294023 Genomic DNA Translation: AAG31593.1 AF294024 Genomic DNA Translation: AAG31594.1 AF294025 Genomic DNA Translation: AAG31595.1 AF294026 Genomic DNA Translation: AAG31596.1 AL133371 Genomic DNA No translation available. BC096060 mRNA Translation: AAH96060.1 BC096061 mRNA Translation: AAH96061.1 BC096062 mRNA Translation: AAH96062.1 AF441204 Genomic DNA Translation: AAL35279.1 AF441205 Genomic DNA Translation: AAL35280.1 AF441206 Genomic DNA Translation: AAL35281.1 |
CCDSi | CCDS9560.1 |
PIRi | B35328, JL0106 |
RefSeqi | NP_002926.2, NM_002935.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DYT | X-ray | 1.75 | A/B | 28-160 | [»] | |
1H1H | X-ray | 2.00 | A | 28-160 | [»] | |
1QMT | X-ray | 2.40 | A | 28-160 | [»] | |
2KB5 | NMR | - | A | 28-160 | [»] | |
2LVZ | NMR | - | A | 28-160 | [»] | |
4A2O | X-ray | 1.69 | A/B | 28-160 | [»] | |
4A2Y | X-ray | 1.70 | A/B | 28-160 | [»] | |
4OWZ | X-ray | 1.47 | A/B | 28-160 | [»] | |
4OXB | X-ray | 1.50 | A/B | 28-160 | [»] | |
4OXF | X-ray | 1.50 | A/B | 28-160 | [»] | |
4X08 | X-ray | 1.34 | A/B | 28-160 | [»] | |
BMRBi | P12724 | |||||
SMRi | P12724 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111966, 13 interactors |
IntActi | P12724, 13 interactors |
STRINGi | 9606.ENSP00000302324 |
Chemistry databases
DrugBanki | DB02098, Adenosine-2'-5'-Diphosphate DB04272, Citric acid DB01411, Pranlukast |
PTM databases
GlyGeni | P12724, 3 sites |
iPTMneti | P12724 |
PhosphoSitePlusi | P12724 |
Genetic variation databases
BioMutai | RNASE3 |
DMDMi | 147744558 |
Proteomic databases
EPDi | P12724 |
jPOSTi | P12724 |
MassIVEi | P12724 |
PaxDbi | P12724 |
PeptideAtlasi | P12724 |
PRIDEi | P12724 |
ProteomicsDBi | 52864 |
Protocols and materials databases
Antibodypediai | 57757, 322 antibodies from 29 providers |
DNASUi | 6037 |
Genome annotation databases
Ensembli | ENST00000304639; ENSP00000302324; ENSG00000169397 |
GeneIDi | 6037 |
KEGGi | hsa:6037 |
MANE-Selecti | ENST00000304639.4; ENSP00000302324.3; NM_002935.3; NP_002926.2 |
UCSCi | uc001vyj.4, human |
Organism-specific databases
CTDi | 6037 |
DisGeNETi | 6037 |
GeneCardsi | RNASE3 |
HGNCi | HGNC:10046, RNASE3 |
HPAi | ENSG00000169397, Tissue enriched (bone) |
MIMi | 131398, gene |
neXtProti | NX_P12724 |
OpenTargetsi | ENSG00000169397 |
PharmGKBi | PA34414 |
VEuPathDBi | HostDB:ENSG00000169397 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502TF52, Eukaryota |
GeneTreei | ENSGT00940000162253 |
HOGENOMi | CLU_117006_0_1_1 |
InParanoidi | P12724 |
OMAi | NQSIRCP |
OrthoDBi | 1482425at2759 |
PhylomeDBi | P12724 |
TreeFami | TF333393 |
Enzyme and pathway databases
PathwayCommonsi | P12724 |
Reactomei | R-HSA-6798695, Neutrophil degranulation R-HSA-6803157, Antimicrobial peptides |
SignaLinki | P12724 |
SIGNORi | P12724 |
Miscellaneous databases
BioGRID-ORCSi | 6037, 6 hits in 1038 CRISPR screens |
EvolutionaryTracei | P12724 |
GeneWikii | Eosinophil_cationic_protein |
GenomeRNAii | 6037 |
Pharosi | P12724, Tbio |
PROi | PR:P12724 |
RNActi | P12724, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000169397, Expressed in bone marrow and 113 other tissues |
Genevisiblei | P12724, HS |
Family and domain databases
Gene3Di | 3.10.130.10, 1 hit |
InterProi | View protein in InterPro IPR001427, RNaseA IPR036816, RNaseA-like_dom_sf IPR023411, RNaseA_AS IPR023412, RNaseA_domain |
PANTHERi | PTHR11437, PTHR11437, 1 hit |
Pfami | View protein in Pfam PF00074, RnaseA, 1 hit |
PRINTSi | PR00794, RIBONUCLEASE |
SMARTi | View protein in SMART SM00092, RNAse_Pc, 1 hit |
SUPFAMi | SSF54076, SSF54076, 1 hit |
PROSITEi | View protein in PROSITE PS00127, RNASE_PANCREATIC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ECP_HUMAN | |
Accessioni | P12724Primary (citable) accession number: P12724 Secondary accession number(s): Q4VBC1 Q9GZN9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | May 15, 2007 | |
Last modified: | February 23, 2022 | |
This is version 200 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families