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Protein

Alcohol dehydrogenase class-3

Gene

Adh5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.

Catalytic activityi

A primary alcohol + NAD+ = an aldehyde + NADH.
A secondary alcohol + NAD+ = a ketone + NADH.
S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45Zinc 1; catalyticBy similarity1
Metal bindingi67Zinc 1; catalyticBy similarity1
Metal bindingi97Zinc 2By similarity1
Metal bindingi100Zinc 2By similarity1
Metal bindingi103Zinc 2By similarity1
Metal bindingi111Zinc 2By similarity1
Sitei115Important for FDH activity and activation by fatty acidsBy similarity1
Metal bindingi174Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • ethanol catabolic process Source: RGD
  • ethanol oxidation Source: InterPro

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-71384 Ethanol oxidation
SABIO-RKiP12711

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase class-3 (EC:1.1.1.1)
Alternative name(s):
Alcohol dehydrogenase 2
Alcohol dehydrogenase 5
Alcohol dehydrogenase B2
Short name:
ADH-B2
Alcohol dehydrogenase class-III
Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-)
Short name:
FALDH
Short name:
FDH
Short name:
GSH-FDH
S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.284)
Gene namesi
Name:Adh5
Synonyms:Adh-2, Adh2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2292706 Adh5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001607622 – 374Alcohol dehydrogenase class-3Add BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine2 Publications1
Modified residuei233N6-succinyllysineBy similarity1
Modified residuei247PhosphoserineBy similarity1
Modified residuei315N6-succinyllysineBy similarity1
Modified residuei324PhosphoserineBy similarity1
Modified residuei351PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP12711
PRIDEiP12711

PTM databases

iPTMnetiP12711
PhosphoSitePlusiP12711

Expressioni

Gene expression databases

BgeeiENSRNOG00000046357
ExpressionAtlasiP12711 baseline and differential
GenevisibleiP12711 RN

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017252

Structurei

3D structure databases

ProteinModelPortaliP12711
SMRiP12711
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0022 Eukaryota
COG1062 LUCA
GeneTreeiENSGT00430000030800
HOGENOMiHOG000294674
HOVERGENiHBG000195
InParanoidiP12711
KOiK00121

Family and domain databases

CDDicd08300 alcohol_DH_class_III, 1 hit
InterProiView protein in InterPro
IPR014183 ADH_3
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SUPFAMiSSF50129 SSF50129, 2 hits
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR02818 adh_III_F_hyde, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12711-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANQVIRCKA AVAWEAGKPL SIEEIEVAPP QAHEVRIKII ATAVCHTDAY
60 70 80 90 100
TLSGADPEGC FPVILGHEGA GIVESVGEGV TKLKAGDTVI PLYIPQCGEC
110 120 130 140 150
KFCLNPKTNL CQKIRVTQGK GLMPDGTSRF TCKGKPILHF MGTSTFSEYT
160 170 180 190 200
VVADISVAKI DPSAPLDKVC LLGCGISTGY GAAVNTAKVE PGSTCAVFGL
210 220 230 240 250
GGVGLAVIMG CKVAGASRII GIDINKDKFA KAKEFGATEC INPQDFSKSI
260 270 280 290 300
QEVLIEMTDG GVDFSFECIG NVKVMRSALE AAHKGWGVSV VVGVAASGEE
310 320 330 340 350
ISTRPFQLVT GRTWKGTAFG GWKSVESVPK LVSEYMSKKI KVDEFVTGNL
360 370
SFDQINKAFD LMHSGNSIRT VLKM
Length:374
Mass (Da):39,576
Last modified:March 20, 2007 - v2
Checksum:iD97A768DEED84C96
GO

Sequence cautioni

The sequence AAH83724 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti374M → L AA sequence (PubMed:3278908).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083724 mRNA Translation: AAH83724.1 Different initiation.
PIRiS00331 DERTA
RefSeqiNP_001119592.1, NM_001126120.1
UniGeneiRn.222115

Genome annotation databases

EnsembliENSRNOT00000017252; ENSRNOP00000017252; ENSRNOG00000046357
GeneIDi100145871
KEGGirno:100145871

Similar proteinsi

Entry informationi

Entry nameiADHX_RAT
AccessioniPrimary (citable) accession number: P12711
Secondary accession number(s): Q5XIF8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: March 20, 2007
Last modified: May 23, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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