UniProtKB - P12689 (REV1_YEAST)
Protein
DNA repair protein REV1
Gene
REV1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.3 Publications
Miscellaneous
Present with 521 molecules/cell in log phase SD medium.1 Publication
Cofactori
Mg2+2 PublicationsNote: Binds 2 magnesium ions.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 324 | dCTP2 Publications | 1 | |
Metal bindingi | 362 | Magnesium 1PROSITE-ProRule annotation1 Publication | 1 | |
Metal bindingi | 362 | Magnesium 2PROSITE-ProRule annotation1 Publication | 1 | |
Metal bindingi | 363 | Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation1 Publication | 1 | |
Binding sitei | 414 | dCTP2 Publications | 1 | |
Metal bindingi | 467 | Magnesium 1PROSITE-ProRule annotation1 Publication | 1 | |
Binding sitei | 467 | dCTP2 Publications | 1 | |
Metal bindingi | 468 | Magnesium 1PROSITE-ProRule annotation1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 362 – 366 | dCTP binding2 Publications | 5 | |
Nucleotide bindingi | 402 – 408 | dCTP binding2 Publications | 7 |
GO - Molecular functioni
- damaged DNA binding Source: InterPro
- deoxycytidyl transferase activity Source: SGD
- DNA-directed DNA polymerase activity Source: SGD
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- error-free translesion synthesis Source: SGD
- error-prone translesion synthesis Source: SGD
Keywordsi
Molecular function | DNA-binding, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair, DNA synthesis |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:REV1 Ordered Locus Names:YOR346W ORF Names:O6339 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005873, REV1 |
VEuPathDBi | FungiDB:YOR346W |
Subcellular locationi
Mitochondrion
Nucleus
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
- replication fork Source: SGD
Keywords - Cellular componenti
Mitochondrion, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 193 | G → R: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 467 – 468 | DE → AA: Loss of dCTP transferase activity. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173995 | 1 – 985 | DNA repair protein REV1Add BLAST | 985 |
Proteomic databases
MaxQBi | P12689 |
PaxDbi | P12689 |
PRIDEi | P12689 |
PTM databases
iPTMneti | P12689 |
Interactioni
Subunit structurei
Interacts with REV7.
1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 681 | Interaction with target DNA | 1 | |
Sitei | 692 | Interaction with target DNA | 1 | |
Sitei | 694 | Interaction with target DNA | 1 |
Binary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 34731, 125 interactors |
DIPi | DIP-1000N |
IntActi | P12689, 6 interactors |
MINTi | P12689 |
STRINGi | 4932.YOR346W |
Miscellaneous databases
RNActi | P12689, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P12689 |
SMRi | P12689 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P12689 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 161 – 249 | BRCTPROSITE-ProRule annotationAdd BLAST | 89 | |
Domaini | 358 – 554 | UmuCPROSITE-ProRule annotationAdd BLAST | 197 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 319 – 329 | Interaction with target DNAAdd BLAST | 11 | |
Regioni | 395 – 397 | Interaction with target DNA | 3 | |
Regioni | 554 – 557 | Interaction with target DNA | 4 | |
Regioni | 620 – 628 | Interaction with target DNA | 9 |
Sequence similaritiesi
Belongs to the DNA polymerase type-Y family.Curated
Phylogenomic databases
eggNOGi | KOG2093, Eukaryota |
GeneTreei | ENSGT00940000156374 |
HOGENOMi | CLU_003901_1_0_1 |
InParanoidi | P12689 |
OMAi | FGDYATY |
Family and domain databases
Gene3Di | 3.30.1490.100, 1 hit 3.30.70.270, 1 hit 3.40.50.10190, 1 hit |
InterProi | View protein in InterPro IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR043502, DNA/RNA_pol_sf IPR036775, DNA_pol_Y-fam_lit_finger_sf IPR017961, DNA_pol_Y-fam_little_finger IPR012112, REV1 IPR043128, Rev_trsase/Diguanyl_cyclase IPR001126, UmuC |
Pfami | View protein in Pfam PF16589, BRCT_2, 1 hit PF00817, IMS, 1 hit PF11799, IMS_C, 1 hit |
PIRSFi | PIRSF036573, REV1, 1 hit |
SMARTi | View protein in SMART SM00292, BRCT, 1 hit |
SUPFAMi | SSF100879, SSF100879, 1 hit SSF52113, SSF52113, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50172, BRCT, 1 hit PS50173, UMUC, 1 hit |
i Sequence
Sequence statusi: Complete.
P12689-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGEHGGLVDL LDSDLEYSIN RETPDKNNCL SQQSVNDSHL TAKTGGLNAR
60 70 80 90 100
SFLSTLSDDS LIEYVNQLSQ TNKNNSNPTA GTLRFTTKNI SCDELHADLG
110 120 130 140 150
GGEDSPIARS VIEIQESDSN GDDVKKNTVY TREAYFHEKA HGQTLQDQIL
160 170 180 190 200
KDQYKDQISS QSSKIFKNCV IYINGYTKPG RLQLHEMIVL HGGKFLHYLS
210 220 230 240 250
SKKTVTHIVA SNLPLKKRIE FANYKVVSPD WIVDSVKEAR LLPWQNYSLT
260 270 280 290 300
SKLDEQQKKL DNCKTVNSIP LPSETSLHKG SKCVGSALLP VEQQSPVNLN
310 320 330 340 350
NLEAKRIVAC DDPDFLTSYF AHSRLHHLSA WKANLKDKFL NENIHKYTKI
360 370 380 390 400
TDKDTYIIFH IDFDCFFATV AYLCRSSSFS ACDFKRDPIV VCHGTKNSDI
410 420 430 440 450
ASCNYVARSY GIKNGMWVSQ AEKMLPNGIK LISLPYTFEQ FQLKSEAFYS
460 470 480 490 500
TLKRLNIFNL ILPISIDEAV CVRIIPDNIH NTNTLNARLC EEIRQEIFQG
510 520 530 540 550
TNGCTVSIGC SDSLVLARLA LKMAKPNGYN ITFKSNLSEE FWSSFKLDDL
560 570 580 590 600
PGVGHSTLSR LESTFDSPHS LNDLRKRYTL DALKASVGSK LGMKIHLALQ
610 620 630 640 650
GQDDEESLKI LYDPKEVLQR KSLSIDINWG IRFKNITQVD LFIERGCQYL
660 670 680 690 700
LEKLNEINKT TSQITLKLMR RCKDAPIEPP KYMGMGRCDS FSRSSRLGIP
710 720 730 740 750
TNEFGIIATE MKSLYRTLGC PPMELRGLAL QFNKLVDVGP DNNQLKLRLP
760 770 780 790 800
FKTIVTNRAF EALPEDVKND INNEFEKRNY KRKESGLTSN SLSSKKKGFA
810 820 830 840 850
ISRLEVNDLP STMEEQFMNE LPTQIRAEVR HDLRIQKKIQ QTKLGNLQEK
860 870 880 890 900
IKRREESLQN EKNHFMGQNS IFQPIKFQNL TRFKKICQLV KQWVAETLGD
910 920 930 940 950
GGPHEKDVKL FVKYLIKLCD SNRVHLVLHL SNLISRELNL CAFLNQDHSG
960 970 980
FQTWERILLN DIIPLLNRNK HTYQTVRKLD MDFEV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 646 | G → S in AAA34967 (PubMed:2492497).Curated | 1 | |
Sequence conflicti | 816 | Q → H in AAA34967 (PubMed:2492497).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M22222 Genomic DNA Translation: AAA34967.1 X95720 Genomic DNA Translation: CAA65033.1 Z75253 Genomic DNA Translation: CAA99674.1 BK006948 Genomic DNA Translation: DAA11107.1 |
PIRi | S67255 |
RefSeqi | NP_014991.1, NM_001183766.1 |
Genome annotation databases
EnsemblFungii | YOR346W_mRNA; YOR346W; YOR346W |
GeneIDi | 854527 |
KEGGi | sce:YOR346W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M22222 Genomic DNA Translation: AAA34967.1 X95720 Genomic DNA Translation: CAA65033.1 Z75253 Genomic DNA Translation: CAA99674.1 BK006948 Genomic DNA Translation: DAA11107.1 |
PIRi | S67255 |
RefSeqi | NP_014991.1, NM_001183766.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2AQ4 | X-ray | 2.32 | A | 305-738 | [»] | |
2M2I | NMR | - | A | 158-251 | [»] | |
3BJY | X-ray | 2.41 | A | 305-738 | [»] | |
3OSP | X-ray | 2.50 | A | 305-738 | [»] | |
4ID3 | X-ray | 1.97 | A/B | 159-250 | [»] | |
5UMV | X-ray | 1.75 | A | 162-251 | [»] | |
5VX7 | NMR | - | A | 162-251 | [»] | |
5WM1 | X-ray | 1.85 | A | 305-738 | [»] | |
5WM8 | X-ray | 1.92 | A | 305-738 | [»] | |
5WMB | X-ray | 2.25 | A | 305-738 | [»] | |
5YRQ | X-ray | 2.00 | A/B/D/E | 876-985 | [»] | |
6X6Z | X-ray | 1.40 | A | 305-746 | [»] | |
6X70 | X-ray | 2.05 | A | 305-746 | [»] | |
6X71 | X-ray | 1.78 | A | 305-746 | [»] | |
6X72 | X-ray | 2.19 | A | 305-746 | [»] | |
6X73 | X-ray | 2.05 | A | 305-746 | [»] | |
6X74 | X-ray | 1.69 | A | 305-746 | [»] | |
6X75 | X-ray | 1.95 | A | 305-746 | [»] | |
6X76 | X-ray | 2.53 | A | 305-746 | [»] | |
6X77 | X-ray | 1.64 | A | 305-746 | [»] | |
BMRBi | P12689 | |||||
SMRi | P12689 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34731, 125 interactors |
DIPi | DIP-1000N |
IntActi | P12689, 6 interactors |
MINTi | P12689 |
STRINGi | 4932.YOR346W |
PTM databases
iPTMneti | P12689 |
Proteomic databases
MaxQBi | P12689 |
PaxDbi | P12689 |
PRIDEi | P12689 |
Genome annotation databases
EnsemblFungii | YOR346W_mRNA; YOR346W; YOR346W |
GeneIDi | 854527 |
KEGGi | sce:YOR346W |
Organism-specific databases
SGDi | S000005873, REV1 |
VEuPathDBi | FungiDB:YOR346W |
Phylogenomic databases
eggNOGi | KOG2093, Eukaryota |
GeneTreei | ENSGT00940000156374 |
HOGENOMi | CLU_003901_1_0_1 |
InParanoidi | P12689 |
OMAi | FGDYATY |
Miscellaneous databases
EvolutionaryTracei | P12689 |
PROi | PR:P12689 |
RNActi | P12689, protein |
Family and domain databases
Gene3Di | 3.30.1490.100, 1 hit 3.30.70.270, 1 hit 3.40.50.10190, 1 hit |
InterProi | View protein in InterPro IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR043502, DNA/RNA_pol_sf IPR036775, DNA_pol_Y-fam_lit_finger_sf IPR017961, DNA_pol_Y-fam_little_finger IPR012112, REV1 IPR043128, Rev_trsase/Diguanyl_cyclase IPR001126, UmuC |
Pfami | View protein in Pfam PF16589, BRCT_2, 1 hit PF00817, IMS, 1 hit PF11799, IMS_C, 1 hit |
PIRSFi | PIRSF036573, REV1, 1 hit |
SMARTi | View protein in SMART SM00292, BRCT, 1 hit |
SUPFAMi | SSF100879, SSF100879, 1 hit SSF52113, SSF52113, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50172, BRCT, 1 hit PS50173, UMUC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | REV1_YEAST | |
Accessioni | P12689Primary (citable) accession number: P12689 Secondary accession number(s): D6W341, Q12323 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names