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Protein

Cholesterol oxidase

Gene

choA

Organism
Streptomyces sp. (strain SA-COO)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product.

Catalytic activityi

Cholesterol + O2 = cholest-5-en-3-one + H2O2.1 Publication
A 3-oxo-Delta5-steroid = a 3-oxo-Delta4-steroid.1 Publication

Cofactori

Pathwayi: cholesterol metabolism

This protein is involved in the pathway cholesterol metabolism, which is part of Steroid metabolism.
View all proteins of this organism that are known to be involved in the pathway cholesterol metabolism and in Steroid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei398Proton acceptor1
Active sitei484Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi54 – 70FADSequence analysisAdd BLAST17

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Oxidoreductase
Biological processCholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism
LigandFAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.1.3.6 1284
UniPathwayi
UPA00296

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol oxidase (EC:1.1.3.6)
Short name:
CHOD
Alternative name(s):
Cholesterol isomerase (EC:5.3.3.1)
Gene namesi
Name:choA
OrganismiStreptomyces sp. (strain SA-COO)
Taxonomic identifieri74576 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi398E → Q: Reduced activity. 1 Publication1
Mutagenesisi484H → N or Q: Reduced activity. 1 Publication1

Chemistry databases

DrugBankiDB03147 Flavin adenine dinucleotide
DB02332 Flavin-N7 Protonated-Adenine Dinucleotide

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 42Tat-type signalAdd BLAST42
ChainiPRO_000001233343 – 546Cholesterol oxidaseAdd BLAST504

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Proteomic databases

PRIDEiP12676

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1546
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP12676
SMRiP12676
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12676

Family & Domainsi

Sequence similaritiesi

Belongs to the GMC oxidoreductase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.50.50.60, 4 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR000172 GMC_OxRdtase_N
IPR007867 GMC_OxRtase_C
IPR006311 TAT_signal
PfamiView protein in Pfam
PF05199 GMC_oxred_C, 1 hit
PF00732 GMC_oxred_N, 1 hit
SUPFAMiSSF51905 SSF51905, 2 hits
PROSITEiView protein in PROSITE
PS00623 GMC_OXRED_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12676-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTAQQHLSRR RMLGMAAFGA AALAGGTTIA APRAAAAAKS AADNGGYVPA
60 70 80 90 100
VVIGTGYGAA VSALRLGEAG VQTLMLEMGQ LWNQPGPDGN IFCGMLNPDK
110 120 130 140 150
RSSWFKNRTE APLGSFLWLD VVNRNIDPYA GVLDRVNYDQ MSVYVGRGVG
160 170 180 190 200
GGSLVNGGMA VEPKRSYFEE ILPRVDSSEM YDRYFPRANS MLRVNHIDTK
210 220 230 240 250
WFEDTEWYKF ARVSREQAGK AGLGTVFVPN VYDFGYMQRE AAGEVPKSAL
260 270 280 290 300
ATEVIYGNNH GKQSLDKTYL AAALGTGKVT IQTLHQVKTI RQTKDGGYAL
310 320 330 340 350
TVEQKDTDGK LLATKEISCR YLFLGAGSLG STELLVRARD TGTLPNLNSE
360 370 380 390 400
VGAGWGPNGN IMTARANHMW NPTGAHQSSI PALGIDAWDN SDSSVFAEIA
410 420 430 440 450
PMPAGLETWV SLYLAITKNP QRGTFVYDAA TDRAKLNWTR DQNAPAVNAA
460 470 480 490 500
KALFDRINKA NGTIYRYDLF GTQLKAFADD FCYHPLGGCV LGKATDDYGR
510 520 530 540
VAGYKNLYVT DGSLIPGSVG VNPFVTITAL AERNVERIIK QDVTAS
Length:546
Mass (Da):58,994
Last modified:October 1, 1989 - v1
Checksum:iEF22A1FE5EA68D21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31939 Genomic DNA Translation: AAA26719.1
PIRiA32260

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31939 Genomic DNA Translation: AAA26719.1
PIRiA32260

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B4VX-ray1.50A43-546[»]
1B8SX-ray1.65A43-546[»]
1CBOX-ray1.80A43-546[»]
1CC2X-ray2.20A43-546[»]
1IJHX-ray1.53A43-546[»]
1MXTX-ray0.95A43-546[»]
1N1PX-ray0.95A43-546[»]
1N4UX-ray0.95A43-546[»]
1N4VX-ray1.00A43-546[»]
1N4WX-ray0.92A43-546[»]
2GEWX-ray0.97A43-546[»]
3B3RX-ray0.98A42-546[»]
3B6DX-ray1.20A43-546[»]
3CNJX-ray0.95A45-543[»]
3GYIX-ray1.00A43-546[»]
3GYJX-ray0.92A43-546[»]
4REKX-ray0.74A46-544[»]
4U2LX-ray1.34A43-546[»]
4U2SX-ray1.12A43-546[»]
4U2TX-ray1.22A43-546[»]
4XWRX-ray1.10A43-546[»]
4XXGX-ray0.85A43-546[»]
5KWFOther1.50A43-546[»]
ProteinModelPortaliP12676
SMRiP12676
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB03147 Flavin adenine dinucleotide
DB02332 Flavin-N7 Protonated-Adenine Dinucleotide

Proteomic databases

PRIDEiP12676

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayi
UPA00296

BRENDAi1.1.3.6 1284

Miscellaneous databases

EvolutionaryTraceiP12676

Family and domain databases

Gene3Di3.50.50.60, 4 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR000172 GMC_OxRdtase_N
IPR007867 GMC_OxRtase_C
IPR006311 TAT_signal
PfamiView protein in Pfam
PF05199 GMC_oxred_C, 1 hit
PF00732 GMC_oxred_N, 1 hit
SUPFAMiSSF51905 SSF51905, 2 hits
PROSITEiView protein in PROSITE
PS00623 GMC_OXRED_1, 1 hit
PS51318 TAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHOD_STRS0
AccessioniPrimary (citable) accession number: P12676
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: February 28, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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