UniProtKB - P12658 (CALB1_MOUSE)
Protein
Calbindin
Gene
Calb1
Organism
Mus musculus (Mouse)
Status
Functioni
Buffers cytosolic calcium. May stimulate a membrane Ca2+-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 24 – 35 | 1By similarityAdd BLAST | 12 | |
Calcium bindingi | 111 – 122 | 2By similarityAdd BLAST | 12 | |
Calcium bindingi | 155 – 166 | 3By similarityAdd BLAST | 12 | |
Calcium bindingi | 199 – 210 | 4By similarityAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: SynGO
- calcium ion binding involved in regulation of postsynaptic cytosolic calcium ion concentration Source: SynGO
- vitamin D binding Source: UniProtKB-KW
- zinc ion binding Source: MGI
GO - Biological processi
- cellular response to organic substance Source: MGI
- cochlea development Source: Ensembl
- locomotory behavior Source: MGI
- long-term memory Source: MGI
- metanephric collecting duct development Source: UniProtKB
- metanephric connecting tubule development Source: UniProtKB
- metanephric distal convoluted tubule development Source: UniProtKB
- metanephric part of ureteric bud development Source: UniProtKB
- regulation of cytosolic calcium ion concentration Source: GO_Central
- regulation of long-term synaptic potentiation Source: SynGO
- regulation of presynaptic cytosolic calcium ion concentration Source: MGI
- regulation of synaptic plasticity Source: MGI
- retina development in camera-type eye Source: UniProtKB
- retina layer formation Source: UniProtKB
- short-term memory Source: MGI
Keywordsi
Ligand | Calcium, Metal-binding, Vitamin D |
Names & Taxonomyi
Protein namesi | Recommended name: CalbindinAlternative name(s): Calbindin D28 D-28K PCD-29 Spot 35 protein Vitamin D-dependent calcium-binding protein, avian-type |
Gene namesi | Name:Calb1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88248, Calb1 |
Subcellular locationi
Cytoskeleton
- cuticular plate Source: MGI
Cytosol
- cytosol Source: MGI
- postsynaptic cytosol Source: MGI
- presynaptic cytosol Source: MGI
Nucleus
- nucleus Source: UniProtKB
Other locations
- axon Source: BHF-UCL
- calyx of Held Source: MGI
- cell body Source: MGI
- cytoplasm Source: MGI
- dendrite Source: MGI
- dendritic spine Source: MGI
- neuron projection Source: MGI
- neuronal cell body Source: MGI
- postsynapse Source: MGI
- stereocilium Source: MGI
- synapse Source: MGI
- terminal bouton Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000073473 | 2 – 261 | CalbindinAdd BLAST | 260 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P12658 |
MaxQBi | P12658 |
PaxDbi | P12658 |
PeptideAtlasi | P12658 |
PRIDEi | P12658 |
PTM databases
iPTMneti | P12658 |
PhosphoSitePlusi | P12658 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000028222, Expressed in molar tooth and 215 other tissues |
Genevisiblei | P12658, MM |
Interactioni
Subunit structurei
Interacts with RANBP9.
By similarityProtein-protein interaction databases
BioGRIDi | 198449, 4 interactors |
IntActi | P12658, 1 interactor |
MINTi | P12658 |
STRINGi | 10090.ENSMUSP00000029876 |
Miscellaneous databases
RNActi | P12658, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 46 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 53 – 88 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 98 – 133 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 142 – 177 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 186 – 221 | EF-hand 5PROSITE-ProRule annotationAdd BLAST | 36 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 7 | Interaction with RANBP9By similarity | 6 |
Domaini
This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.By similarity
Sequence similaritiesi
Belongs to the calbindin family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0027, Eukaryota |
GeneTreei | ENSGT00950000183108 |
HOGENOMi | CLU_054826_1_1_1 |
InParanoidi | P12658 |
OMAi | WLHFDSD |
OrthoDBi | 979297at2759 |
PhylomeDBi | P12658 |
TreeFami | TF325083 |
Family and domain databases
InterProi | View protein in InterPro IPR029634, Calbindin IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom |
PANTHERi | PTHR19972:SF14, PTHR19972:SF14, 1 hit |
Pfami | View protein in Pfam PF00036, EF-hand_1, 1 hit PF13499, EF-hand_7, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 4 hits |
SUPFAMi | SSF47473, SSF47473, 2 hits |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 4 hits PS50222, EF_HAND_2, 5 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P12658-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAESHLQSSL ITASQFFEIW LHFDADGSGY LEGKELQNLI QELLQARKKA
60 70 80 90 100
GLELSPEMKS FVDQYGQRDD GKIGIVELAH VLPTEENFLL LFRCQQLKSC
110 120 130 140 150
EEFMKTWRKY DTDHSGFIET EELKNFLKDL LEKANKTVDD TKLAEYTDLM
160 170 180 190 200
LKLFDSNNDG KLELTEMARL LPVQENFLLK FQGIKMCGKE FNKAFELYDQ
210 220 230 240 250
DGNGYIDENE LDALLKDLCE KNKQELDINN ITTYKKNIMA LSDGGKLYRT
260
DLALILSAGD N
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21531 mRNA Translation: AAA39898.1 D26352 Genomic DNA Translation: BAA05386.1 AK002635 mRNA Translation: BAB22249.1 AK005081 mRNA Translation: BAB23805.1 AK005243 mRNA Translation: BAB23900.1 AK028050 mRNA Translation: BAC25721.1 AK048517 mRNA Translation: BAC33354.1 AK077499 mRNA Translation: BAC36831.1 BC016421 mRNA Translation: AAH16421.1 M23663 mRNA Translation: AAA53198.1 |
CCDSi | CCDS17984.1 |
PIRi | A34955 |
RefSeqi | NP_033918.1, NM_009788.4 |
Genome annotation databases
Ensembli | ENSMUST00000029876; ENSMUSP00000029876; ENSMUSG00000028222 |
GeneIDi | 12307 |
KEGGi | mmu:12307 |
UCSCi | uc008sbl.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21531 mRNA Translation: AAA39898.1 D26352 Genomic DNA Translation: BAA05386.1 AK002635 mRNA Translation: BAB22249.1 AK005081 mRNA Translation: BAB23805.1 AK005243 mRNA Translation: BAB23900.1 AK028050 mRNA Translation: BAC25721.1 AK048517 mRNA Translation: BAC33354.1 AK077499 mRNA Translation: BAC36831.1 BC016421 mRNA Translation: AAH16421.1 M23663 mRNA Translation: AAA53198.1 |
CCDSi | CCDS17984.1 |
PIRi | A34955 |
RefSeqi | NP_033918.1, NM_009788.4 |
3D structure databases
SMRi | P12658 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198449, 4 interactors |
IntActi | P12658, 1 interactor |
MINTi | P12658 |
STRINGi | 10090.ENSMUSP00000029876 |
PTM databases
iPTMneti | P12658 |
PhosphoSitePlusi | P12658 |
Proteomic databases
jPOSTi | P12658 |
MaxQBi | P12658 |
PaxDbi | P12658 |
PeptideAtlasi | P12658 |
PRIDEi | P12658 |
Protocols and materials databases
ABCDi | P12658, 2 sequenced antibodies |
Antibodypediai | 3678, 443 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000029876; ENSMUSP00000029876; ENSMUSG00000028222 |
GeneIDi | 12307 |
KEGGi | mmu:12307 |
UCSCi | uc008sbl.1, mouse |
Organism-specific databases
CTDi | 793 |
MGIi | MGI:88248, Calb1 |
Phylogenomic databases
eggNOGi | KOG0027, Eukaryota |
GeneTreei | ENSGT00950000183108 |
HOGENOMi | CLU_054826_1_1_1 |
InParanoidi | P12658 |
OMAi | WLHFDSD |
OrthoDBi | 979297at2759 |
PhylomeDBi | P12658 |
TreeFami | TF325083 |
Miscellaneous databases
BioGRID-ORCSi | 12307, 0 hits in 17 CRISPR screens |
PROi | PR:P12658 |
RNActi | P12658, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028222, Expressed in molar tooth and 215 other tissues |
Genevisiblei | P12658, MM |
Family and domain databases
InterProi | View protein in InterPro IPR029634, Calbindin IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom |
PANTHERi | PTHR19972:SF14, PTHR19972:SF14, 1 hit |
Pfami | View protein in Pfam PF00036, EF-hand_1, 1 hit PF13499, EF-hand_7, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 4 hits |
SUPFAMi | SSF47473, SSF47473, 2 hits |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 4 hits PS50222, EF_HAND_2, 5 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CALB1_MOUSE | |
Accessioni | P12658Primary (citable) accession number: P12658 Secondary accession number(s): Q545N6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 183 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families