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Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Measles virus (strain Edmonston) (MeV) (Subacute sclerose panencephalitis virus)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.By similarity
RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.By similarity
5'-triphospho-mRNA + GDP = diphosphate + guanosine 5'-triphospho-mRNA.By similarity
S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(2'-O-methyl-ribonucleotide)-[mRNA].By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1785 – 1794ATPSequence analysis10

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Multifunctional enzyme, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase
Biological processmRNA capping, mRNA processing, Viral RNA replication
LigandATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 4 domains:
RNA-directed RNA polymerase (EC:2.7.7.48By similarity)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56By similarity)
GDP polyribonucleotidyltransferase (EC:2.7.7.88By similarity)
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 (EC:2.1.1.296By similarity)
Gene namesi
Name:L
OrganismiMeasles virus (strain Edmonston) (MeV) (Subacute sclerose panencephalitis virus)
Taxonomic identifieri11235 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeMorbillivirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000000833 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • host cell cytoplasm Source: UniProtKB-SubCell
  • viral nucleocapsid Source: CACAO

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001427291 – 2183RNA-directed RNA polymerase LAdd BLAST2183

Proteomic databases

PRIDEiP12576

Interactioni

Subunit structurei

Interacts with the P protein.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini656 – 840RdRp catalyticPROSITE-ProRule annotationAdd BLAST185
Domaini1755 – 1958Mononegavirus-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd BLAST204

Sequence similaritiesi

Belongs to the paramyxovirus L protein family.Curated

Phylogenomic databases

OrthoDBiVOG09000005

Family and domain databases

InterProiView protein in InterPro
IPR026890 Mononeg_mRNAcap
IPR014023 Mononeg_RNA_pol_cat
IPR025786 Mononega_L_MeTrfase
IPR016269 RNA-dir_pol_paramyxovirus
IPR024352 RNA-pol_paramyxovirus_C_dom
PfamiView protein in Pfam
PF12803 G-7-MTase, 1 hit
PF14318 Mononeg_mRNAcap, 1 hit
PF00946 Mononeg_RNA_pol, 1 hit
PIRSFiPIRSF000830 RNA_pol_ParamyxoV, 1 hit
TIGRFAMsiTIGR04198 paramyx_RNAcap, 1 hit
PROSITEiView protein in PROSITE
PS50526 RDRP_SSRNA_NEG_NONSEG, 1 hit
PS51590 SAM_MT_MNV_L, 1 hit

Sequencei

Sequence statusi: Complete.

P12576-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDSLSVNQIL YPEVHLDSPI VTNKIVAILE YARVPHAYSL EDPTLCQNIK
60 70 80 90 100
HRLKNGFSNQ MIINNVEVGN VIKSKLRSYP AHSHIPYPNC NQDLFNIEDK
110 120 130 140 150
ESTRKIRELL KKGNSLYSKV SDKVFQCLRD TNSRLGLGSE LREDIKEKVI
160 170 180 190 200
NLGVYMHSSQ WFEPFLFWFT VKTEMRSVIK SQTHTCHRRR HTPVFFTGSS
210 220 230 240 250
VELLISRDLV AIISKESQHV YYLTFELVLM YCDVIEGRLM TETAMTIDAR
260 270 280 290 300
YTELLGRVRY MWKLIDGFFP ALGNPTYQIV AMLEPLSLAY LQLRDITVEL
310 320 330 340 350
RGAFLNHCFT EIHDVLDQNG FSDEGTYHEL IEALDYIFIT DDIHLTGEIF
360 370 380 390 400
SFFRSFGHPR LEAVTAAENV RKYMNQPKVI VYETLMKGHA IFCGIIINGY
410 420 430 440 450
RDRHGGSWPP LTLPLHAADT IRNAQASGEG LTHEQCVDNW KSFAGVKFGC
460 470 480 490 500
FMPLSLDSDL TMYLKDKALA ALQREWDSVY PKEFLRYDPP KGTGSRRLVD
510 520 530 540 550
VFLNDSSFDP YDVIMYVVSG AYLHDPEFNL SYSLQEKEIK ETGRLFAKMT
560 570 580 590 600
YKMRACQVIA ENLISNGIGK YFKDNGMAKD EQDLTKALHT LAVSGVPKDL
610 620 630 640 650
KESHRGGPVL KTYSRSPVHT STRNVRAAKG FIGFPQVIRQ DQDTDHPENM
660 670 680 690 700
EAYETVSAFI TTDLKKYCLN WRYETISLFA QRLNEIYGLP SFFQWLHKRL
710 720 730 740 750
ETSVLYVSDP HCPPDLDAHI PLYKVPNDQI FIKYPMGGIE GYCQKLWTIS
760 770 780 790 800
TIPYLYLAAY ESGVRIASLV QGDNQTIAVT KRVPSTWPYN LKKREAARVT
810 820 830 840 850
RDYFVILRQR LHDIGHHLKA NETIVSSHFF VYSKGIYYDG LLVSQSLKSI
860 870 880 890 900
ARCVFWSETI VDETRAACSN IATTMAKSIE RGYDRYLAYS LNFLKVIQQI
910 920 930 940 950
LISLGFTINS TMTRDVVIPL LTNNDLLIRM ALLPAPIGGM NYLNMSRLFV
960 970 980 990 1000
RNIGDPVTSS IADLKRMILA SLMPEETLHQ VMTQQPGDSS FLDWASDPYS
1010 1020 1030 1040 1050
ANLVCVQSIT RLLKNITARF VLIHSPNPML KGLFHDDSKE EDEGLAAFLM
1060 1070 1080 1090 1100
DRHIIVPRAA HEILDHSVTG ARESIAGMLD TTKGLIRASM RKGGLTSRVI
1110 1120 1130 1140 1150
TRLSNYDYEQ FRAGMVLLTG RKRNVLIDKE SCSVQLARAL RSHMWARLAR
1160 1170 1180 1190 1200
GRPIYGLEVP DVLESMRGHL IRRHETCVIC ECGSVNYGWF FVPSGCQLDD
1210 1220 1230 1240 1250
IDKETSSLRV PYIGSTTDER TDMKLAFVRA PSRSLRSAVR IATVYSWAYG
1260 1270 1280 1290 1300
DDDSSWNEAW LLARQRANVS LEELRVITPI STSTNLAHRL RDRSTQVKYS
1310 1320 1330 1340 1350
GTSLVRVARY TTISNDNLSF VISDKKVDTN FIYQQGMLLG LGVLETLFRL
1360 1370 1380 1390 1400
EKDTGSSNTV LHLHVETDCC VIPMIDHPRI PSSRKLELRA ELCTNPLIYD
1410 1420 1430 1440 1450
NAPLIDRDAT RLYTQSHRRH LVEFVTWSTP QLYHILAKST ALSMIDLVTK
1460 1470 1480 1490 1500
FEKDHMNEIS ALIGDDDINS FITEFLLIEP RLFTIYLGQC AAINWAFDVH
1510 1520 1530 1540 1550
YHRPSGKYQM GELLSSFLSR MSKGVFKVLV NALSHPKIYK KFWHCGIIEP
1560 1570 1580 1590 1600
IHGPSLDAQN LHTTVCNMVY TCYMTYLDLL LNEELEEFTF LLCESDEDVV
1610 1620 1630 1640 1650
PDRFDNIQAK HLCVLADLYC QPGTCPPIQG LRPVEKCAVL TDHIKAEAML
1660 1670 1680 1690 1700
SPAGSSWNIN PIIVDHYSCS LTYLRRGSIK QIRLRVDPGF IFDALAEVNV
1710 1720 1730 1740 1750
SQPKIGSNNI SNMSIKAFRP PHDDVAKLLK DINTSKHNLP ISGGNLANYE
1760 1770 1780 1790 1800
IHAFRRIGLN SSACYKAVEI STLIRRCLEP GEDGLFLGEG SGSMLITYKE
1810 1820 1830 1840 1850
ILKLSKCFYN SGVSANSRSG QRELAPYPSE VGLVEHRMGV GNIVKVLFNG
1860 1870 1880 1890 1900
RPEVTWVGSV DCFNFIVSNI PTSSVGFIHS DIETLPDKDT IEKLEELAAI
1910 1920 1930 1940 1950
LSMALLLGKI GSILVIKLMP FSGDFVQGFI SYVGSHYREV NLVYPRYSNF
1960 1970 1980 1990 2000
ISTESYLVMT DLKANRLMNP EKIKQQIIES SVRTSPGLIG HILSIKQLSC
2010 2020 2030 2040 2050
IQAIVGDAVS RGDINPTLKK LTPIEQVLIN CGLAINGPKL CKELIHHDVA
2060 2070 2080 2090 2100
SGQDGLLNSI LILYRELARF KDNQRSQQGM FHAYPVLVSS RQRELISRIT
2110 2120 2130 2140 2150
RKFWGHILLY SGNRKLINKF IQNLKSGYLI LDLHQNIFVK NLSKSEKQII
2160 2170 2180
MTGGLKREWV FKVTVKETKE WYKLVGYSAL IKD
Length:2,183
Mass (Da):247,648
Last modified:October 1, 1989 - v1
Checksum:i1B0B03CA2E2B6EA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20865 mRNA Translation: AAA46430.1
K01711 Genomic RNA Translation: AAA75501.1
PIRiA28919 ZLNZMV

Similar proteinsi

Entry informationi

Entry nameiL_MEASE
AccessioniPrimary (citable) accession number: P12576
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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