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Protein

Hemagglutinin-neuraminidase

Gene

HN

Organism
Newcastle disease virus (strain Chicken/Australia-Victoria/32) (NDV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity).By similarity
Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.By similarity

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHemagglutinin, Hydrolase
Biological processHost-virus interaction, Viral attachment to host cell, Virus entry into host cell

Protein family/group databases

CAZyiGH83 Glycoside Hydrolase Family 83

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-neuraminidase (EC:3.2.1.18)
Gene namesi
Name:HN
OrganismiNewcastle disease virus (strain Chicken/Australia-Victoria/32) (NDV)
Taxonomic identifieri11177 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeAvulavirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 26IntravirionSequence analysisAdd BLAST26
Transmembranei27 – 48HelicalSequence analysisAdd BLAST22
Topological domaini49 – 570Virion surfaceSequence analysisAdd BLAST522

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001426061 – 570Hemagglutinin-neuraminidaseAdd BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi119N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi340N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi432N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi480N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi507N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi537N-linked (GlcNAc...) asparagine; by hostSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP12554

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-15941682,EBI-15941682

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-60382N

Structurei

Secondary structure

1570
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP12554
SMRiP12554
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12554

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

CDDicd15469 HN, 1 hit
InterProiView protein in InterPro
IPR016285 Hemagglutn-neuramid
IPR000665 Hemagglutn/HN
IPR036278 Sialidase_sf
PfamiView protein in Pfam
PF00423 HN, 1 hit
PIRSFiPIRSF001072 Hemagglut-neuramid_paramyxoV, 1 hit
SUPFAMiSSF50939 SSF50939, 1 hit

Sequencei

Sequence statusi: Complete.

P12554-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNRAVCQVAL ENDEREAKNT WRLVFRIAIL LLTVMTLAIS AAALAYSMEA
60 70 80 90 100
STPGDLVSIP TAISRAEGKI TSALGSNQDV VDRIYKQVAL ESPLALLNTE
110 120 130 140 150
SIIMNAITSL SYQINGAANN SGCGAPVHDP DYIGGIGKEL IVDDTSDVTS
160 170 180 190 200
FYPSAFQEHL NFIPAPTTGS GCTRIPSFDM SATHCYTHNV IFSGCRDHSH
210 220 230 240 250
SHQYLALGVL RTSATGRVFF STLRSINLDD TQNRKSCSVS ATPLGCDMLC
260 270 280 290 300
SKVTETEEED YNSVIPTSMV HGRLGFDGQY HEKDLDVTTL FGDWVANYPG
310 320 330 340 350
VGGGSFIDNR VWFPVYGGLK PSSPSDTGQE GRYVIYKRYN DTCPDEQDYQ
360 370 380 390 400
IRMAKSSYKP GRFGGKRVQQ AILSIKVSTS LGEDPVLTIP PNTVTLMGAE
410 420 430 440 450
GRVLTVGTSH FLYQRGSSYF SPALLYPMTV NNNTATLHSP YTFNAFTRPG
460 470 480 490 500
SVPCQASARC PNSCVTGVYT DPYPLVFHRN HTLRGVFGTM LDDEQARLNL
510 520 530 540 550
VSAVFDNISR SRITRVSSSR TKAAYTTSTC FKVVKTNKTY CLSIAEISNT
560 570
LFGEFRIVPL LVEILKDDGV
Length:570
Mass (Da):62,284
Last modified:June 1, 1994 - v2
Checksum:i96EF3FFED67179D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184H → HY in AAA46662 (PubMed:2705297).Curated1
Sequence conflicti197D → H in AAA46670 (PubMed:3037818).Curated1
Sequence conflicti259E → Q in AAA46670 (PubMed:3037818).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22110 mRNA Translation: AAA46670.1
M24712 Genomic RNA Translation: AAA46662.1
PIRiB31110 HNNZAV

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22110 mRNA Translation: AAA46670.1
M24712 Genomic RNA Translation: AAA46662.1
PIRiB31110 HNNZAV

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T1EX-ray3.30A/B/E/F49-570[»]
ProteinModelPortaliP12554
SMRiP12554
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60382N

Protein family/group databases

CAZyiGH83 Glycoside Hydrolase Family 83

Proteomic databases

PRIDEiP12554

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP12554

Family and domain databases

CDDicd15469 HN, 1 hit
InterProiView protein in InterPro
IPR016285 Hemagglutn-neuramid
IPR000665 Hemagglutn/HN
IPR036278 Sialidase_sf
PfamiView protein in Pfam
PF00423 HN, 1 hit
PIRSFiPIRSF001072 Hemagglut-neuramid_paramyxoV, 1 hit
SUPFAMiSSF50939 SSF50939, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHN_NDVA
AccessioniPrimary (citable) accession number: P12554
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: June 1, 1994
Last modified: September 12, 2018
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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