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UniProtKB - P12547 (ORYZ_ASPOR)
Protein
Alkaline protease 1
Gene
alp1
Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Functioni
Secreted alkaline protease that allows assimilation of proteinaceous substrates.
1 PublicationCatalytic activityi
- Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyze peptide amides. EC:3.4.21.63
pH dependencei
Optimum pH is 9.1 Publication
Temperature dependencei
Optimum temperature is 40 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 162 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 193 | Charge relay systemPROSITE-ProRule annotation | 1 | |
Active sitei | 349 | Charge relay systemPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BRENDAi | 3.4.21.63, 522 |
Protein family/group databases
MEROPSi | S08.053 |
Names & Taxonomyi
Protein namesi | Recommended name: Alkaline protease 1 (EC:3.4.21.63)Short name: ALP Alternative name(s): Aspergillopeptidase B Aspergillus proteinase B Elastase Elastinolytic serine proteinase Oryzin |
Gene namesi | Name:alp1 Synonyms:alk1, alp, alpA ORF Names:AO090003001036 |
Organismi | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
Taxonomic identifieri | 510516 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Circumdati › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:AO090003001036 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Add BLAST | 21 | |
PropeptideiPRO_0000027084 | 22 – 121 | Add BLAST | 100 | |
ChainiPRO_0000027085 | 122 – 403 | Alkaline protease 1Add BLAST | 282 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 253 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, ZymogenExpressioni
Inductioni
Expression is controlled by the prtT transcription factor.1 Publication
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 120 | Inhibitor I9Sequence analysisAdd BLAST | 85 | |
Domaini | 130 – 403 | Peptidase S8PROSITE-ProRule annotationAdd BLAST | 274 |
Sequence similaritiesi
Belongs to the peptidase S8 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
HOGENOMi | CLU_011263_1_4_1 |
OMAi | GEGVWAY |
Family and domain databases
CDDi | cd04077, Peptidases_S8_PCSK9_ProteinaseK_like, 1 hit |
Gene3Di | 3.30.70.80, 1 hit 3.40.50.200, 1 hit |
InterProi | View protein in InterPro IPR034193, PCSK9_ProteinaseK-like IPR000209, Peptidase_S8/S53_dom IPR036852, Peptidase_S8/S53_dom_sf IPR023827, Peptidase_S8_Asp-AS IPR022398, Peptidase_S8_His-AS IPR023828, Peptidase_S8_Ser-AS IPR015500, Peptidase_S8_subtilisin-rel IPR010259, S8pro/Inhibitor_I9 IPR037045, S8pro/Inhibitor_I9_sf |
Pfami | View protein in Pfam PF05922, Inhibitor_I9, 1 hit PF00082, Peptidase_S8, 1 hit |
PRINTSi | PR00723, SUBTILISIN |
SUPFAMi | SSF52743, SSF52743, 1 hit |
PROSITEi | View protein in PROSITE PS51892, SUBTILASE, 1 hit PS00136, SUBTILASE_ASP, 1 hit PS00137, SUBTILASE_HIS, 1 hit PS00138, SUBTILASE_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P12547-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQSIKRTLLL LGAILPAVLG APVQETRRAA EKLPGKYIVT FKPGIDEAKI
60 70 80 90 100
QEHTTWATNI HQRSLERRGA TGGDLPVGIE RNYKINKFAA YAGSFDDATI
110 120 130 140 150
EEIRKNEDVA YVEEDQIYYL DGLTTQKSAP WGLGSISHKG QQSTDYIYDT
160 170 180 190 200
SAGEGTYAYV VDSGVNVDHE EFEGRASKAY NAAGGQHVDS IGHGTHVSGT
210 220 230 240 250
IAGKTYGIAK KASILSVKVF QGESSSTSVI LDGFNWAAND IVSKKRTSKA
260 270 280 290 300
AINMSLGGGY SKAFNDAVEN AFEQGVLSVV AAGNENSDAG QTSPASAPDA
310 320 330 340 350
ITVAAIQKSN NRASFSNFGK VVDVFAPGQD ILSAWIGSSS ATNTISGTSM
360 370 380 390 400
ATPHIVGLSL YLAALENLDG PAAVTKRIKE LATKDVVKDV KGSPNLLAYN
GNA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17561 mRNA Translation: CAA35594.1 S75278 Genomic DNA Translation: AAB20819.1 X54726 Genomic DNA Translation: CAA38527.2 S79617 Genomic DNA Translation: AAC60533.1 D10062 Genomic DNA Translation: BAA00951.1 AP007155 Genomic DNA Translation: BAE58142.1 D00350 mRNA Translation: BAA00258.1 |
PIRi | JU0278, SUASO |
RefSeqi | XP_001820144.1, XM_001820092.2 |
Genome annotation databases
EnsemblFungii | BAE58142; BAE58142; AO090003001036 |
GeneIDi | 5992127 |
KEGGi | aor:AO090003001036 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17561 mRNA Translation: CAA35594.1 S75278 Genomic DNA Translation: AAB20819.1 X54726 Genomic DNA Translation: CAA38527.2 S79617 Genomic DNA Translation: AAC60533.1 D10062 Genomic DNA Translation: BAA00951.1 AP007155 Genomic DNA Translation: BAE58142.1 D00350 mRNA Translation: BAA00258.1 |
PIRi | JU0278, SUASO |
RefSeqi | XP_001820144.1, XM_001820092.2 |
3D structure databases
SMRi | P12547 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 510516.P12547 |
Protein family/group databases
Allergomei | 3132, Asp o 13.0101 84, Asp o 13 |
MEROPSi | S08.053 |
Genome annotation databases
EnsemblFungii | BAE58142; BAE58142; AO090003001036 |
GeneIDi | 5992127 |
KEGGi | aor:AO090003001036 |
Organism-specific databases
VEuPathDBi | FungiDB:AO090003001036 |
Phylogenomic databases
HOGENOMi | CLU_011263_1_4_1 |
OMAi | GEGVWAY |
Enzyme and pathway databases
BRENDAi | 3.4.21.63, 522 |
Family and domain databases
CDDi | cd04077, Peptidases_S8_PCSK9_ProteinaseK_like, 1 hit |
Gene3Di | 3.30.70.80, 1 hit 3.40.50.200, 1 hit |
InterProi | View protein in InterPro IPR034193, PCSK9_ProteinaseK-like IPR000209, Peptidase_S8/S53_dom IPR036852, Peptidase_S8/S53_dom_sf IPR023827, Peptidase_S8_Asp-AS IPR022398, Peptidase_S8_His-AS IPR023828, Peptidase_S8_Ser-AS IPR015500, Peptidase_S8_subtilisin-rel IPR010259, S8pro/Inhibitor_I9 IPR037045, S8pro/Inhibitor_I9_sf |
Pfami | View protein in Pfam PF05922, Inhibitor_I9, 1 hit PF00082, Peptidase_S8, 1 hit |
PRINTSi | PR00723, SUBTILISIN |
SUPFAMi | SSF52743, SSF52743, 1 hit |
PROSITEi | View protein in PROSITE PS51892, SUBTILASE, 1 hit PS00136, SUBTILASE_ASP, 1 hit PS00137, SUBTILASE_HIS, 1 hit PS00138, SUBTILASE_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ORYZ_ASPOR | |
Accessioni | P12547Primary (citable) accession number: P12547 Secondary accession number(s): Q2UJX3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | February 1, 1991 | |
Last modified: | February 23, 2022 | |
This is version 148 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families