Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neuronal acetylcholine receptor subunit beta-4

Gene

Chrnb4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei81Key residue that facilitates effective access of the conotoxin BuIA to the channel binding site1 Publication1
Sitei133Key residue for a low dissociation (K(off)) from the conotoxin BuIA1 Publication1
Sitei141Key residue for a low dissociation (K(off)) from the conotoxin BuIA1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-629587 Highly sodium permeable acetylcholine nicotinic receptors
R-RNO-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
R-RNO-629597 Highly calcium permeable nicotinic acetylcholine receptors
R-RNO-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit beta-4
Alternative name(s):
Neuronal acetylcholine receptor non-alpha-2 chain
Short name:
N-alpha 2
Gene namesi
Name:Chrnb4
Synonyms:Acrb4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi2351 Chrnb4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 235ExtracellularSequence analysisAdd BLAST215
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Topological domaini257 – 264CytoplasmicSequence analysis8
Transmembranei265 – 285HelicalSequence analysisAdd BLAST21
Topological domaini286 – 297ExtracellularSequence analysisAdd BLAST12
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Topological domaini319 – 463CytoplasmicSequence analysisAdd BLAST145
Transmembranei464 – 484HelicalSequence analysisAdd BLAST21
Topological domaini485 – 495ExtracellularSequence analysisAdd BLAST11

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2658

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000000039121 – 495Neuronal acetylcholine receptor subunit beta-4Add BLAST475

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi92N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi137N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi152 ↔ 166By similarity
Glycosylationi165N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP12392
PRIDEiP12392

PTM databases

PhosphoSitePlusiP12392

Expressioni

Tissue specificityi

In the brain, it is detected in the medial habenula. In the peripheral nervous system, it is found at least in the adrenal gland.

Gene expression databases

BgeeiENSRNOG00000014427 Expressed in 4 organ(s), highest expression level in colon
GenevisibleiP12392 RN

Interactioni

Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Beta-4 subunit can be combined to alpha-2, alpha-3 or alpha-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly (By similarity). Interacts with LYPD6 (By similarity). The pentamer alpha3-beta-4 interacts with the conotoxin BuIA (PubMed:16964981). The heteropentamer composed of alpha-3 and beta-4 subunits interacts with the alpha-conotoxin ImI (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Chrna4P094834EBI-9008856,EBI-7842410

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-183 Neuronal nicotinic acetylcholine receptor complex, alpha2-beta4
CPX-205 Neuronal nicotinic acetylcholine receptor complex, alpha3-beta4
CPX-208 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta4
CPX-211 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta4
CPX-219 Neuronal nicotinic acetylcholine receptor complex, alpha4-beta4
IntActiP12392, 4 interactors
STRINGi10116.ENSRNOP00000051052

Chemistry databases

BindingDBiP12392

Structurei

3D structure databases

ProteinModelPortaliP12392
SMRiP12392
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00760000118930
HOGENOMiHOG000006757
HOVERGENiHBG003756
InParanoidiP12392
KOiK04815
OMAiFMKRPEN
OrthoDBiEOG091G0R20

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform Short (identifier: P12392-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRGTPLLLVS LFSLLQDGDC RLANAEEKLM DDLLNKTRYN NLIRPATSSS
60 70 80 90 100
QLISIRLELS LSQLISVNER EQIMTTSIWL KQEWTDYRLA WNSSCYEGVN
110 120 130 140 150
ILRIPAKRVW LPDIVLYNNA DGTYEVSVYT NVIVRSNGSI QWLPPAIYKS
160 170 180 190 200
ACKIEVKHFP FDQQNCTLKF RSWTYDHTEI DMVLKSPTAI MDDFTPSGEW
210 220 230 240 250
DIVALPGRRT VNPQDPSYVD VTYDFIIKRK PLFYTINLII PCVLITSLAI
260 270 280 290 300
LVFYLPSDCG EKMTLCISVL LALTFFLLLI SKIVPPTSLD IPLIGKYLLF
310 320 330 340 350
TMVLVTFSIV TTVCVLNVHH RSPSTHTMAS WVKECFLHKL PTFLFMKRPG
360 370 380 390 400
LEVSLVRVPH PSQLHLATAD TAATSALGPT SPSNLYGSSM YFVNPVPAAP
410 420 430 440 450
KSAVSSHTAG LPRDARLRSS GRFREDLQEA LEGVSFIAQH LESDDRDQSV
460 470 480 490
IEDWKFVAMV VDRLFLWVFV FVCILGTMGL FLPPLFQIHA PSKDS
Length:495
Mass (Da):55,861
Last modified:October 1, 1989 - v1
Checksum:i688F37AB80448F7F
GO
Isoform Long (identifier: P12392-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     17-17: D → DARLFDCSGVLPDKGPAGLTVRFP

Show »
Length:518
Mass (Da):58,260
Checksum:i61946BD129580D29
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9V → VV in CAA33839 (PubMed:2918319).Curated1
Sequence conflicti164Missing in AAA41668 (PubMed:1689727).Curated1
Sequence conflicti230 – 231KP → NA in AAA41668 (PubMed:1689727).Curated2
Sequence conflicti230 – 231KP → NA in CAA33839 (PubMed:2918319).Curated2
Sequence conflicti287 – 288Missing in CAA33839 (PubMed:2918319).Curated2
Sequence conflicti290D → N in CAA33839 (PubMed:2918319).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00007617D → DARLFDCSGVLPDKGPAGLT VRFP in isoform Long. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42976 mRNA Translation: AAA85212.1
M33953
, J05232, M89971, M33951, M89989, M33952 Genomic DNA Translation: AAA41668.1
X15834 mRNA Translation: CAA33839.1
AY574260 mRNA Translation: AAS90356.1
PIRiA30992
B35721
RefSeqiNP_434693.1, NM_052806.2 [P12392-1]
UniGeneiRn.9695

Genome annotation databases

EnsembliENSRNOT00000019458; ENSRNOP00000019458; ENSRNOG00000014427 [P12392-1]
GeneIDi25103
KEGGirno:25103
UCSCiRGD:2351 rat [P12392-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42976 mRNA Translation: AAA85212.1
M33953
, J05232, M89971, M33951, M89989, M33952 Genomic DNA Translation: AAA41668.1
X15834 mRNA Translation: CAA33839.1
AY574260 mRNA Translation: AAS90356.1
PIRiA30992
B35721
RefSeqiNP_434693.1, NM_052806.2 [P12392-1]
UniGeneiRn.9695

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OLJmodel-B/D/E25-231[»]
ProteinModelPortaliP12392
SMRiP12392
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-183 Neuronal nicotinic acetylcholine receptor complex, alpha2-beta4
CPX-205 Neuronal nicotinic acetylcholine receptor complex, alpha3-beta4
CPX-208 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta4
CPX-211 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta4
CPX-219 Neuronal nicotinic acetylcholine receptor complex, alpha4-beta4
IntActiP12392, 4 interactors
STRINGi10116.ENSRNOP00000051052

Chemistry databases

BindingDBiP12392
ChEMBLiCHEMBL2658

PTM databases

PhosphoSitePlusiP12392

Proteomic databases

PaxDbiP12392
PRIDEiP12392

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019458; ENSRNOP00000019458; ENSRNOG00000014427 [P12392-1]
GeneIDi25103
KEGGirno:25103
UCSCiRGD:2351 rat [P12392-1]

Organism-specific databases

CTDi1143
RGDi2351 Chrnb4

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00760000118930
HOGENOMiHOG000006757
HOVERGENiHBG003756
InParanoidiP12392
KOiK04815
OMAiFMKRPEN
OrthoDBiEOG091G0R20

Enzyme and pathway databases

ReactomeiR-RNO-629587 Highly sodium permeable acetylcholine nicotinic receptors
R-RNO-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
R-RNO-629597 Highly calcium permeable nicotinic acetylcholine receptors
R-RNO-6798695 Neutrophil degranulation

Miscellaneous databases

PROiPR:P12392

Gene expression databases

BgeeiENSRNOG00000014427 Expressed in 4 organ(s), highest expression level in colon
GenevisibleiP12392 RN

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACHB4_RAT
AccessioniPrimary (citable) accession number: P12392
Secondary accession number(s): Q63361
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 7, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again