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Protein

Neuronal acetylcholine receptor subunit beta-2

Gene

Chrnb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodiun ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei83Key residue that may interfere with effective access of the conotoxin BuIA to the channel binding site1 Publication1
Sitei118Key residue that may play an important stabilizing role for the interaction with alpha-conotoxins PnIA, GID and MII, allowing them to bind deep into the nAChR cleft1 Publication1
Sitei135Key residue for a rapid dissociation (K(off)) from the conotoxin BuIA1 Publication1
Sitei143Key residue for a rapid dissociation (K(off)) from the conotoxin BuIA1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-629587 Highly sodium permeable acetylcholine nicotinic receptors
R-RNO-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
R-RNO-629597 Highly calcium permeable nicotinic acetylcholine receptors

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit beta-2
Alternative name(s):
Neuronal acetylcholine receptor non-alpha-1 chain
Short name:
N-alpha 1
Gene namesi
Name:Chrnb2
Synonyms:Acrb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2350 Chrnb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 237ExtracellularSequence analysisAdd BLAST213
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Topological domaini259 – 266CytoplasmicSequence analysis8
Transmembranei267 – 287HelicalSequence analysisAdd BLAST21
Topological domaini288 – 299ExtracellularSequence analysisAdd BLAST12
Transmembranei300 – 320HelicalSequence analysisAdd BLAST21
Topological domaini321 – 458CytoplasmicSequence analysisAdd BLAST138
Transmembranei459 – 479HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi83T → D: 9-fold increase in affinity to the conotoxin BuIA (15-fold decrease in K(off)). 1 Publication1
Mutagenesisi83T → G: 12-fold increase in affinity to the conotoxin BuIA (11-fold decrease in K(off)). 1 Publication1
Mutagenesisi83T → K: 20-fold increase in affinity to the conotoxin BuIA (37-fold decrease in K(off)); 2-fold decrease in affinity to the conotoxin MII (no change in K(off), but 2-fold decrease in K(on)). 1 Publication1
Mutagenesisi83T → S: 19-fold increase in affinity to the conotoxin BuIA (7-fold decrease in K(off)). 1 Publication1
Mutagenesisi83T → V: 3-fold increase in affinity to the conotoxin BuIA (2-fold decrease in K(off)). 1 Publication1
Mutagenesisi118L → Q: 40-fold, 165-fold, and 300-fold decrease in inhibition of alpha-3-beta-2(L118Q) nAChR by alpha-conotoxins PnIA, GID and MII, respectively. 1 Publication1
Mutagenesisi135V → A: Very small decrease or no change in inhibition of alpha-3-beta-2(V135A) nAChR by alpha-conotoxins PnIA, GID and MII. 1 Publication1
Mutagenesisi135V → G: No change in inhibition of alpha-3-beta-2(V135G) nAChR by alpha-conotoxins GID and MII. 4.4-fold decrease in inhibition of alpha-3-beta-2(V135G) nAChR by alpha-conotoxins PnIA. 1 Publication1
Mutagenesisi135V → I: 8.3-fold decrease in affinity to the conotoxin BuIA (4-fold increase in K(off) and a 2-fold decrease in K(on)). 1 Publication1
Mutagenesisi143F → A: No change in inhibition of alpha-3-beta-2(F143A) nAChR by alpha-conotoxins PnIA, GID and MII. 12-fold and 6.6-fold increase in inhibition of alpha-4-beta-2(F143A) nAChR by alpha-conotoxins GID and MII, respectively, but no change in inhibition of the same receptor by alpha-conotoxin PnIA. 1 Publication1
Mutagenesisi143F → Q: 6.6-fold increase in affinity to the conotoxin BuIA (7-fold decrease in K(off)). 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3137275

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000000038125 – 500Neuronal acetylcholine receptor subunit beta-2Add BLAST476

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi50N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi154 ↔ 168By similarity
Glycosylationi167N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP12390
PRIDEiP12390

PTM databases

SwissPalmiP12390

Expressioni

Tissue specificityi

Expressed in most regions of the CNS.

Gene expression databases

BgeeiENSRNOG00000020778 Expressed in 7 organ(s), highest expression level in brain
GenevisibleiP12390 RN

Interactioni

Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Beta-2 subunit can be combined to alpha-2, alpha-3 or alpha-4 to give rise to functional receptors, complexes with beta-2 may be heteropentamers. Alpha-2/4:beta-2 nAChR complexes are proposed to exist in two subtypes: LS (low agonist sensitivity) with a (alpha-2/4)3:(beta-2)2 and HS (high agonist sensitivity) with a (alpha-2/4)2:(beta-2)3 stoichiometry; the subtypes differ in their subunit binding interfaces which are involved in ligand binding. Interacts with RIC3; which is required for proper folding and assembly. Interacts with LYPD6. The heteropentamer alpha3-beta-2 interacts with alpha-conotoxins BuIA, MII, ImI, ImII, PnIA and GID (PubMed:15609996, PubMed:15929983, PubMed:16964981). The heteropentamer alpha-4-beta-2 interacts with the alpha-conotoxins PnIA, GID and MII (PubMed:15929983).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Chrna4P094836EBI-9008812,EBI-7842410

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-170 Neuronal nicotinic acetylcholine receptor complex, 2xalpha4-3xbeta2
CPX-171 Neuronal nicotinic acetylcholine receptor complex, 3xalpha4-2xbeta2
CPX-178 Neuronal nicotinic acetylcholine receptor complex, alpha2-beta2
CPX-190 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta2
CPX-191 Neuronal nicotinic acetylcholine receptor complex, alpha3-beta2
CPX-203 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta2-beta3
CPX-215 Neuronal nicotinic acetylcholine receptor complex, alpha4-alpha5-beta2
CPX-237 Neuronal nicotinic acetylcholine receptor complex, alpha7-beta2
CORUMiP12390
IntActiP12390, 4 interactors
STRINGi10116.ENSRNOP00000028200

Chemistry databases

BindingDBiP12390

Structurei

3D structure databases

ProteinModelPortaliP12390
SMRiP12390
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00760000118930
HOGENOMiHOG000006757
HOVERGENiHBG003756
InParanoidiP12390
KOiK04813
OMAiPAHYNKL
OrthoDBiEOG091G0R20
PhylomeDBiP12390
TreeFamiTF315605

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR032932 CHRNB2
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PTHR18945:SF80 PTHR18945:SF80, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12390-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGHSNSMAL FSFSLLWLCS GVLGTDTEER LVEHLLDPSR YNKLIRPATN
60 70 80 90 100
GSELVTVQLM VSLAQLISVH EREQIMTTNV WLTQEWEDYR LTWKPEDFDN
110 120 130 140 150
MKKVRLPSKH IWLPDVVLYN NADGMYEVSF YSNAVVSYDG SIFWLPPAIY
160 170 180 190 200
KSACKIEVKH FPFDQQNCTM KFRSWTYDRT EIDLVLKSDV ASLDDFTPSG
210 220 230 240 250
EWDIIALPGR RNENPDDSTY VDITYDFIIR RKPLFYTINL IIPCVLITSL
260 270 280 290 300
AILVFYLPSD CGEKMTLCIS VLLALTVFLL LISKIVPPTS LDVPLVGKYL
310 320 330 340 350
MFTMVLVTFS IVTSVCVLNV HHRSPTTHTM APWVKVVFLE KLPTLLFLQQ
360 370 380 390 400
PRHRCARQRL RLRRRQRERE GAGALFFREG PAADPCTCFV NPASVQGLAG
410 420 430 440 450
AFRAEPTAAG PGRSVGPCSC GLREAVDGVR FIADHMRSED DDQSVREDWK
460 470 480 490 500
YVAMVIDRLF LWIFVFVCVF GTVGMFLQPL FQNYTATTFL HPDHSAPSSK
Length:500
Mass (Da):56,909
Last modified:May 30, 2000 - v2
Checksum:i54C007A48225931C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31622 mRNA Translation: AAC78724.1
AY574258 mRNA Translation: AAS90354.1
PIRiJH0174
RefSeqiNP_062170.1, NM_019297.1
UniGeneiRn.53978

Genome annotation databases

EnsembliENSRNOT00000028200; ENSRNOP00000028200; ENSRNOG00000020778
GeneIDi54239
KEGGirno:54239
UCSCiRGD:2350 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31622 mRNA Translation: AAC78724.1
AY574258 mRNA Translation: AAS90354.1
PIRiJH0174
RefSeqiNP_062170.1, NM_019297.1
UniGeneiRn.53978

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OLEmodel-B/D/E27-233[»]
1OLFmodel-B/D/E27-233[»]
ProteinModelPortaliP12390
SMRiP12390
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-170 Neuronal nicotinic acetylcholine receptor complex, 2xalpha4-3xbeta2
CPX-171 Neuronal nicotinic acetylcholine receptor complex, 3xalpha4-2xbeta2
CPX-178 Neuronal nicotinic acetylcholine receptor complex, alpha2-beta2
CPX-190 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta2
CPX-191 Neuronal nicotinic acetylcholine receptor complex, alpha3-beta2
CPX-203 Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta2-beta3
CPX-215 Neuronal nicotinic acetylcholine receptor complex, alpha4-alpha5-beta2
CPX-237 Neuronal nicotinic acetylcholine receptor complex, alpha7-beta2
CORUMiP12390
IntActiP12390, 4 interactors
STRINGi10116.ENSRNOP00000028200

Chemistry databases

BindingDBiP12390
ChEMBLiCHEMBL3137275

PTM databases

SwissPalmiP12390

Proteomic databases

PaxDbiP12390
PRIDEiP12390

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028200; ENSRNOP00000028200; ENSRNOG00000020778
GeneIDi54239
KEGGirno:54239
UCSCiRGD:2350 rat

Organism-specific databases

CTDi1141
RGDi2350 Chrnb2

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00760000118930
HOGENOMiHOG000006757
HOVERGENiHBG003756
InParanoidiP12390
KOiK04813
OMAiPAHYNKL
OrthoDBiEOG091G0R20
PhylomeDBiP12390
TreeFamiTF315605

Enzyme and pathway databases

ReactomeiR-RNO-629587 Highly sodium permeable acetylcholine nicotinic receptors
R-RNO-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
R-RNO-629597 Highly calcium permeable nicotinic acetylcholine receptors

Miscellaneous databases

PROiPR:P12390

Gene expression databases

BgeeiENSRNOG00000020778 Expressed in 7 organ(s), highest expression level in brain
GenevisibleiP12390 RN

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR032932 CHRNB2
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PTHR18945:SF80 PTHR18945:SF80, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACHB2_RAT
AccessioniPrimary (citable) accession number: P12390
Secondary accession number(s): Q53YK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: May 30, 2000
Last modified: November 7, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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