Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neuronal acetylcholine receptor subunit alpha-2

Gene

Chrna2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
R-RNO-629597 Highly calcium permeable nicotinic acetylcholine receptors

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-2
Gene namesi
Name:Chrna2
Synonyms:Acra2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi621533 Chrna2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 241ExtracellularSequence analysisAdd BLAST214
Transmembranei242 – 266HelicalSequence analysisAdd BLAST25
Transmembranei274 – 292HelicalSequence analysisAdd BLAST19
Transmembranei308 – 329HelicalSequence analysisAdd BLAST22
Topological domaini330 – 484CytoplasmicSequence analysisAdd BLAST155
Transmembranei485 – 503HelicalSequence analysisAdd BLAST19

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2584
GuidetoPHARMACOLOGYi463

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000000034328 – 511Neuronal acetylcholine receptor subunit alpha-2Add BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi56N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi106N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi160 ↔ 174By similarity
Glycosylationi212N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi224 ↔ 225Associated with receptor activationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP12389
PRIDEiP12389

PTM databases

PhosphoSitePlusiP12389

Expressioni

Gene expression databases

BgeeiENSRNOG00000017424 Expressed in 6 organ(s), highest expression level in adult mammalian kidney
GenevisibleiP12389 RN

Interactioni

Subunit structurei

Neuronal AChR seems to be composed of two different types of subunits: alpha and non-alpha (beta). Alpha-2 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors. The alpha-2:beta-2 nAChR complex is proposed to be a heteropentamer with two subtypes: LS (low agonist sensitivity) with a (alpha-2)3:(beta-2)2 and HS (high agonist sensitivity) with a (alpha-2)2:(beta-2)3 stoichiometry; the subtypes differ in their subunit binding interfaces which are involved in ligand binding.By similarity

Protein-protein interaction databases

ComplexPortaliCPX-178 Neuronal nicotinic acetylcholine receptor complex, alpha2-beta2
CPX-183 Neuronal nicotinic acetylcholine receptor complex, alpha2-beta4
STRINGi10116.ENSRNOP00000023475

Chemistry databases

BindingDBiP12389

Structurei

3D structure databases

ProteinModelPortaliP12389
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi391 – 402Poly-GluAdd BLAST12

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00760000118930
HOGENOMiHOG000006756
HOVERGENiHBG003756
InParanoidiP12389
KOiK04804
OMAiSYHWLET
OrthoDBiEOG091G0R20
PhylomeDBiP12389
TreeFamiTF315605

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12389-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTLSHSALQF WTHLYLWCLL LVPAVLTQQG SHTHAEDRLF KHLFGGYNRW
60 70 80 90 100
ARPVPNTSDV VIVRFGLSIA QLIDVDEKNQ MMTTNVWLKQ EWNDYKLRWD
110 120 130 140 150
PAEFGNVTSL RVPSEMIWIP DIVLYNNADG EFAVTHMTKA HLFFTGTVHW
160 170 180 190 200
VPPAIYKSSC SIDVTFFPFD QQNCKMKFGS WTYDKAKIDL EQMERTVDLK
210 220 230 240 250
DYWESGEWAI INATGTYNSK KYDCCAEIYP DVTYYFVIRR LPLFYTINLI
260 270 280 290 300
IPCLLISCLT VLVFYLPSEC GEKITLCISV LLSLTVFLLL ITEIIPSTSL
310 320 330 340 350
VIPLIGEYLL FTMIFVTLSI VITVFVLNVH HRSPSTHNMP NWVRVALLGR
360 370 380 390 400
VPRWLMMNRP LPPMELHGSP DLKLSPSYHW LETNMDAGER EETEEEEEEE
410 420 430 440 450
DENICVCAGL PDSSMGVLYG HGGLHLRAME PETKTPSQAS EILLSPQIQK
460 470 480 490 500
ALEGVHYIAD RLRSEDADSS VKEDWKYVAM VVDRIFLWLF IIVCFLGTIG
510
LFLPPFLAGM I
Length:511
Mass (Da):58,611
Last modified:July 15, 1998 - v2
Checksum:i3824E83BB01D613B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti494C → S in AAA40664 (PubMed:2832952).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10077 mRNA Translation: AAB60900.1
M20297
, M20292, M20293, M20294, M20295, M20296 Genomic DNA Translation: AAA40664.1
AY574253 mRNA Translation: AAS90349.1
PIRiA40110
RefSeqiNP_596911.1, NM_133420.1
UniGeneiRn.9713

Genome annotation databases

EnsembliENSRNOT00000023475; ENSRNOP00000023475; ENSRNOG00000017424
GeneIDi170945
KEGGirno:170945
UCSCiRGD:621533 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10077 mRNA Translation: AAB60900.1
M20297
, M20292, M20293, M20294, M20295, M20296 Genomic DNA Translation: AAA40664.1
AY574253 mRNA Translation: AAS90349.1
PIRiA40110
RefSeqiNP_596911.1, NM_133420.1
UniGeneiRn.9713

3D structure databases

ProteinModelPortaliP12389
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-178 Neuronal nicotinic acetylcholine receptor complex, alpha2-beta2
CPX-183 Neuronal nicotinic acetylcholine receptor complex, alpha2-beta4
STRINGi10116.ENSRNOP00000023475

Chemistry databases

BindingDBiP12389
ChEMBLiCHEMBL2584
GuidetoPHARMACOLOGYi463

PTM databases

PhosphoSitePlusiP12389

Proteomic databases

PaxDbiP12389
PRIDEiP12389

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023475; ENSRNOP00000023475; ENSRNOG00000017424
GeneIDi170945
KEGGirno:170945
UCSCiRGD:621533 rat

Organism-specific databases

CTDi1135
RGDi621533 Chrna2

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00760000118930
HOGENOMiHOG000006756
HOVERGENiHBG003756
InParanoidiP12389
KOiK04804
OMAiSYHWLET
OrthoDBiEOG091G0R20
PhylomeDBiP12389
TreeFamiTF315605

Enzyme and pathway databases

ReactomeiR-RNO-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
R-RNO-629597 Highly calcium permeable nicotinic acetylcholine receptors

Miscellaneous databases

PROiPR:P12389

Gene expression databases

BgeeiENSRNOG00000017424 Expressed in 6 organ(s), highest expression level in adult mammalian kidney
GenevisibleiP12389 RN

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACHA2_RAT
AccessioniPrimary (citable) accession number: P12389
Secondary accession number(s): O08952, Q53YK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 15, 1998
Last modified: November 7, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again