Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Eukaryotic peptide chain release factor subunit 1

Gene

SUP45

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.By similarity1 Publication

Miscellaneous

Present with 13100 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • translation release factor activity Source: SGD
  • translation release factor activity, codon specific Source: SGD

GO - Biological processi

  • DNA-templated transcription, termination Source: SGD
  • translational termination Source: SGD

Keywordsi

Biological processProtein biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-29097-MONOMER
ReactomeiR-SCE-72764 Eukaryotic Translation Termination
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic peptide chain release factor subunit 1
Short name:
Eukaryotic release factor 1
Short name:
eRF1
Alternative name(s):
Omnipotent suppressor protein 1
Gene namesi
Name:SUP45
Synonyms:SAL4, SUP1
Ordered Locus Names:YBR143C
ORF Names:YBR1120
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR143C
SGDiS000000347 SUP45

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001431671 – 437Eukaryotic peptide chain release factor subunit 1Add BLAST437

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei182N5-methylglutamine2 Publications1
Cross-linki331Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei421PhosphoserineCombined sources1

Post-translational modificationi

N5-methylated on Gln-182 by MTQ2.2 Publications

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP12385
PaxDbiP12385
PRIDEiP12385

PTM databases

iPTMnetiP12385

Interactioni

Subunit structurei

Heterodimer of two subunits, one of which binds GTP. Interacts with TPA1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32843, 113 interactors
ComplexPortaliCPX-435 Translation release factor ERF1-ERF3 complex
DIPiDIP-800N
IntActiP12385, 55 interactors
MINTiP12385
STRINGi4932.YBR143C

Structurei

3D structure databases

ProteinModelPortaliP12385
SMRiP12385
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic release factor 1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000009004
HOGENOMiHOG000224681
InParanoidiP12385
KOiK03265
OMAiGPGTEKM
OrthoDBiEOG092C2S9V

Family and domain databases

Gene3Di3.30.1330.30, 1 hit
3.30.960.10, 1 hit
InterProiView protein in InterPro
IPR005140 eRF1_1_Pelota
IPR005141 eRF1_2
IPR005142 eRF1_3
IPR029064 L30e-like
IPR004403 Peptide_chain-rel_eRF1/aRF1
IPR024049 Release_factor_eRF1/aRF1_N
PANTHERiPTHR10113 PTHR10113, 1 hit
PfamiView protein in Pfam
PF03463 eRF1_1, 1 hit
PF03464 eRF1_2, 1 hit
PF03465 eRF1_3, 1 hit
SMARTiView protein in SMART
SM01194 eRF1_1, 1 hit
SUPFAMiSSF55315 SSF55315, 1 hit
SSF55481 SSF55481, 1 hit
TIGRFAMsiTIGR03676 aRF1/eRF1, 1 hit

Sequencei

Sequence statusi: Complete.

P12385-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNEVEKNIE IWKVKKLVQS LEKARGNGTS MISLVIPPKG QIPLYQKMLT
60 70 80 90 100
DEYGTASNIK SRVNRLSVLS AITSTQQKLK LYNTLPKNGL VLYCGDIITE
110 120 130 140 150
DGKEKKVTFD IEPYKPINTS LYLCDNKFHT EVLSELLQAD DKFGFIVMDG
160 170 180 190 200
QGTLFGSVSG NTRTVLHKFT VDLPKKHGRG GQSALRFARL REEKRHNYVR
210 220 230 240 250
KVAEVAVQNF ITNDKVNVKG LILAGSADFK TDLAKSELFD PRLACKVISI
260 270 280 290 300
VDVSYGGENG FNQAIELSAE ALANVKYVQE KKLLEAYFDE ISQDTGKFCY
310 320 330 340 350
GIDDTLKALD LGAVEKLIVF ENLETIRYTF KDAEDNEVIK FAEPEAKDKS
360 370 380 390 400
FAIDKATGQE MDVVSEEPLI EWLAANYKNF GATLEFITDK SSEGAQFVTG
410 420 430
FGGIGAMLRY KVNFEQLVDE SEDEYYDEDE GSDYDFI
Length:437
Mass (Da):49,006
Last modified:October 5, 2010 - v2
Checksum:i9B59133EE3B1F61E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41Q → L in CAA27719 (PubMed:3526282).Curated1
Sequence conflicti41Q → L in M28042 (PubMed:3037308).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04082 Genomic DNA Translation: CAA27719.1
M28042 Genomic DNA No translation available.
Z36012 Genomic DNA Translation: CAA85101.1
X73531 Genomic DNA Translation: CAA51935.1
BK006936 Genomic DNA Translation: DAA07259.1
PIRiS46014
RefSeqiNP_009701.3, NM_001178491.3

Genome annotation databases

EnsemblFungiiYBR143C; YBR143C; YBR143C
GeneIDi852440
KEGGisce:YBR143C

Similar proteinsi

Entry informationi

Entry nameiERF1_YEAST
AccessioniPrimary (citable) accession number: P12385
Secondary accession number(s): D6VQD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 5, 2010
Last modified: July 18, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health