UniProtKB - P12345 (AATM_RABIT)
Protein
Aspartate aminotransferase, mitochondrial
Gene
GOT2
Organism
Oryctolagus cuniculus (Rabbit)
Status
Functioni
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.By similarity
Miscellaneous
In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.
Catalytic activityi
Cofactori
pyridoxal 5'-phosphateBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 65 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 162 | SubstrateBy similarity | 1 | |
Binding sitei | 215 | SubstrateBy similarity | 1 | |
Binding sitei | 407 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- kynurenine-oxoglutarate transaminase activity Source: UniProtKB-EC
- L-aspartate:2-oxoglutarate aminotransferase activity Source: UniProtKB
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- 2-oxoglutarate metabolic process Source: UniProtKB
- aspartate metabolic process Source: UniProtKB
- biosynthetic process Source: InterPro
- glutamate metabolic process Source: UniProtKB
- lipid transport Source: UniProtKB-KW
- protein folding Source: HGNC
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Lipid transport, Transport |
Ligand | Pyridoxal phosphate |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate aminotransferase, mitochondrial (EC:2.6.1.1, EC:2.6.1.7)Short name: mAspAT Alternative name(s): Fatty acid-binding protein Short name: FABP-1 Glutamate oxaloacetate transaminase 2 Kynurenine aminotransferase 4 Kynurenine aminotransferase IV Kynurenine--oxoglutarate transaminase 4 Kynurenine--oxoglutarate transaminase IV Plasma membrane-associated fatty acid-binding protein Short name: FABPpm Transaminase A |
Gene namesi | Name:GOT2 |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion matrix By similarity
Plasma membrane
- Cell membrane By similarity
Mitochondrion
- mitochondrial matrix Source: UniProtKB-SubCell
- mitochondrion Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 29 | Mitochondrion1 PublicationAdd BLAST | 29 | |
ChainiPRO_0000123886 | 30 – 430 | Aspartate aminotransferase, mitochondrialAdd BLAST | 401 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 48 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 59 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 73 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 73 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 82 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 90 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 90 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 96 | 3'-nitrotyrosine; alternateBy similarity | 1 | |
Modified residuei | 96 | Phosphotyrosine; alternateBy similarity | 1 | |
Modified residuei | 122 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 122 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 143 | PhosphoserineBy similarity | 1 | |
Modified residuei | 159 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 159 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 185 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 185 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 227 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 234 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 279 | N6-(pyridoxal phosphate)lysine; alternateBy similarity | 1 | |
Modified residuei | 279 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 296 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 296 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 302 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 309 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 309 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 313 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 338 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 338 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 345 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 363 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 363 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 364 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 387 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 396 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 396 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 404 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 404 | N6-succinyllysine; alternateBy similarity | 1 |
Keywords - PTMi
Acetylation, Methylation, Nitration, PhosphoproteinExpressioni
Gene expression databases
Bgeei | ENSOCUG00000003871, Expressed in heart and 15 other tissues |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 9986.ENSOCUP00000003357 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1411, Eukaryota |
HOGENOMi | CLU_032440_1_2_1 |
OMAi | VGACTIV |
OrthoDBi | 1104596at2759 |
TreeFami | TF300641 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P12345-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALLHSARVL SGVASAFHPG LAAAASARAS SWWAHVEMGP PDPILGVTEA
60 70 80 90 100
YKRDTNSKKM NLGVGAYRDD NGKPYVLPSV RKAEAQIAAK GLDKEYLPIG
110 120 130 140 150
GLAEFCRASA ELALGENSEV VKSGRFVTVQ TISGTGALRI GASFLQRFFK
160 170 180 190 200
FSRDVFLPKP SWGNHTPIFR DAGMQLQSYR YYDPKTCGFD FTGALEDISK
210 220 230 240 250
IPEQSVLLLH ACAHNPTGVD PRPEQWKEIA TVVKKRNLFA FFDMAYQGFA
260 270 280 290 300
SGDGDKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTVICKDADE
310 320 330 340 350
AKRVESQLKI LIRPMYSNPP IHGARIASTI LTSPDLRKQW LQEVKGMADR
360 370 380 390 400
IIGMRTQLVS NLKKEGSTHS WQHITDQIGM FCFTGLKPEQ VERLTKEFSI
410 420 430
YMTKDGRISV AGVTSGNVGY LAHAIHQVTK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAGW02052878 Genomic DNA No translation available. |
PIRi | B27103 |
RefSeqi | XP_002711597.1, XM_002711551.3 |
Genome annotation databases
GeneIDi | 100348732 |
KEGGi | ocu:100348732 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAGW02052878 Genomic DNA No translation available. |
PIRi | B27103 |
RefSeqi | XP_002711597.1, XM_002711551.3 |
3D structure databases
SMRi | P12345 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000003357 |
Genome annotation databases
GeneIDi | 100348732 |
KEGGi | ocu:100348732 |
Organism-specific databases
CTDi | 2806 |
Phylogenomic databases
eggNOGi | KOG1411, Eukaryota |
HOGENOMi | CLU_032440_1_2_1 |
OMAi | VGACTIV |
OrthoDBi | 1104596at2759 |
TreeFami | TF300641 |
Gene expression databases
Bgeei | ENSOCUG00000003871, Expressed in heart and 15 other tissues |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AATM_RABIT | |
Accessioni | P12345Primary (citable) accession number: P12345 Secondary accession number(s): G1SKL2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | September 18, 2013 | |
Last modified: | December 2, 2020 | |
This is version 129 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - UniProtKB entry view manual
User manual for the UniProtKB entry view