GOT2

Functionⁱ

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids (By similarity).By similarity

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Catalytic activityⁱ

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.

L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate.

Cofactorⁱ

pyridoxal 5'-phosphateBy similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	65	Substrate; via amide nitrogenBy similarity	1
Binding siteⁱ	162	SubstrateBy similarity	1
Binding siteⁱ	215	SubstrateBy similarity	1
Binding siteⁱ	407	SubstrateBy similarity	1

GO - Molecular functionⁱ

kynurenine-oxoglutarate transaminase activity Source: UniProtKB-EC
L-aspartate:2-oxoglutarate aminotransferase activity Source: UniProtKB
pyridoxal phosphate binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

2-oxoglutarate metabolic process Source: UniProtKB
aspartate biosynthetic process Source: Ensembl
aspartate catabolic process Source: Ensembl
aspartate metabolic process Source: UniProtKB
fatty acid transport Source: Ensembl
glutamate catabolic process to 2-oxoglutarate Source: Ensembl
glutamate catabolic process to aspartate Source: Ensembl
glutamate metabolic process Source: UniProtKB
oxaloacetate metabolic process Source: Ensembl
protein folding
Source: HGNC
Traceable author statementⁱ
- 9683573
response to ethanol Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Aminotransferase, Transferase
Biological process	Lipid transport, Transport
Ligand	Pyridoxal phosphate

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Aspartate aminotransferase, mitochondrial (EC:2.6.1.1, EC:2.6.1.7) Short name: mAspAT Alternative name(s): Fatty acid-binding protein Short name: FABP-1 Glutamate oxaloacetate transaminase 2 Kynurenine aminotransferase 4 Kynurenine aminotransferase IV Kynurenine--oxoglutarate transaminase 4 Kynurenine--oxoglutarate transaminase IV Plasma membrane-associated fatty acid-binding protein Short name: FABPpm Transaminase A
Gene namesⁱ	Name:GOT2
Organismⁱ	Oryctolagus cuniculus (Rabbit)
Taxonomic identifierⁱ	9986 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus
Proteomesⁱ	UP000001811 Componentⁱ: Chromosome 5

Keywords - Cellular componentⁱ

Cell membrane, Membrane, Mitochondrion

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transit peptideⁱ	1 – 29	Mitochondrion1 PublicationAdd BLAST		29
ChainⁱPRO_0000123886	30 – 430	Aspartate aminotransferase, mitochondrialAdd BLAST		401

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	48	PhosphothreonineBy similarity	1
Modified residueⁱ	59	N6-acetyllysineBy similarity	1
Modified residueⁱ	73	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	73	N6-succinyllysine; alternateBy similarity	1
Modified residueⁱ	82	N6-acetyllysineBy similarity	1
Modified residueⁱ	90	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	90	N6-succinyllysine; alternateBy similarity	1
Modified residueⁱ	96	Nitrated tyrosine; alternateBy similarity	1
Modified residueⁱ	96	Phosphotyrosine; alternateBy similarity	1
Modified residueⁱ	122	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	122	N6-succinyllysine; alternateBy similarity	1
Modified residueⁱ	143	PhosphoserineBy similarity	1
Modified residueⁱ	159	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	159	N6-succinyllysine; alternateBy similarity	1
Modified residueⁱ	185	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	185	N6-succinyllysine; alternateBy similarity	1
Modified residueⁱ	227	N6-succinyllysineBy similarity	1
Modified residueⁱ	234	N6-acetyllysineBy similarity	1
Modified residueⁱ	279	N6-(pyridoxal phosphate)lysine; alternateBy similarity	1
Modified residueⁱ	279	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	296	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	296	N6-succinyllysine; alternateBy similarity	1
Modified residueⁱ	302	N6-acetyllysineBy similarity	1
Modified residueⁱ	309	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	309	N6-succinyllysine; alternateBy similarity	1
Modified residueⁱ	313	Asymmetric dimethylarginineBy similarity	1
Modified residueⁱ	338	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	338	N6-succinyllysine; alternateBy similarity	1
Modified residueⁱ	345	N6-acetyllysineBy similarity	1
Modified residueⁱ	363	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	363	N6-succinyllysine; alternateBy similarity	1
Modified residueⁱ	364	N6-acetyllysineBy similarity	1
Modified residueⁱ	387	N6-acetyllysineBy similarity	1
Modified residueⁱ	396	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	396	N6-succinyllysine; alternateBy similarity	1
Modified residueⁱ	404	N6-acetyllysine; alternateBy similarity	1
Modified residueⁱ	404	N6-succinyllysine; alternateBy similarity	1

Keywords - PTMⁱ

Acetylation, Methylation, Nitration, Phosphoprotein

Proteomic databases

PRIDEⁱ	P12345

PRIDEⁱ

P12345

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSOCUG00000003871 Expressed in 3 organ(s), highest expression level in liver

Bgeeⁱ

ENSOCUG00000003871 Expressed in 3 organ(s), highest expression level in liver

Interactionⁱ

Subunit structureⁱ

Homodimer.By similarity

Protein-protein interaction databases

STRINGⁱ	9986.ENSOCUP00000003357

STRINGⁱ

9986.ENSOCUP00000003357

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P12345
SMRⁱ	P12345
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated

Keywords - Domainⁱ

Transit peptide

Phylogenomic databases

eggNOGⁱ	KOG1411 Eukaryota COG1448 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000014081
HOGENOMⁱ	HOG000185898
KEGG Orthology (KO) More...KOⁱ	K14455
OMAⁱ	RVGACTI
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G06G3
TreeFamⁱ	TF300641

Family and domain databases

Gene3Dⁱ	3.40.640.10, 1 hit 3.90.1150.10, 2 hits
InterProⁱ	View protein in InterPro IPR004839 Aminotransferase_I/II IPR000796 Asp_trans IPR004838 NHTrfase_class1_PyrdxlP-BS IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major
PANTHERⁱ	PTHR11879 PTHR11879, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00155 Aminotran_1_2, 1 hit
PRINTSⁱ	PR00799 TRANSAMINASE
SUPFAMⁱ	SSF53383 SSF53383, 1 hit
PROSITEⁱ	View protein in PROSITE PS00105 AA_TRANSFER_CLASS_1, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P12345-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MALLHSARVL SGVASAFHPG LAAAASARAS SWWAHVEMGP PDPILGVTEA 
        60         70         80         90        100
YKRDTNSKKM NLGVGAYRDD NGKPYVLPSV RKAEAQIAAK GLDKEYLPIG 
       110        120        130        140        150
GLAEFCRASA ELALGENSEV VKSGRFVTVQ TISGTGALRI GASFLQRFFK 
       160        170        180        190        200
FSRDVFLPKP SWGNHTPIFR DAGMQLQSYR YYDPKTCGFD FTGALEDISK 
       210        220        230        240        250
IPEQSVLLLH ACAHNPTGVD PRPEQWKEIA TVVKKRNLFA FFDMAYQGFA 
       260        270        280        290        300
SGDGDKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTVICKDADE 
       310        320        330        340        350
AKRVESQLKI LIRPMYSNPP IHGARIASTI LTSPDLRKQW LQEVKGMADR 
       360        370        380        390        400
IIGMRTQLVS NLKKEGSTHS WQHITDQIGM FCFTGLKPEQ VERLTKEFSI 
       410        420        430
YMTKDGRISV AGVTSGNVGY LAHAIHQVTK

Length:430

Mass (Da):47,409

Last modified:September 18, 2013 - v2

Checksum:ⁱ12F54284974D27A5

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AAGW02052878 Genomic DNA No translation available.
PIRⁱ	B27103
NCBI Reference Sequences More...RefSeqⁱ	XP_002711597.1, XM_002711551.3

Genome annotation databases

Ensemblⁱ	ENSOCUT00000003872; ENSOCUP00000003357; ENSOCUG00000003871
GeneIDⁱ	100348732
KEGGⁱ	ocu:100348732

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P12345	Aspartate aminotransferase, mitochondrial		RAT		430	UniRef90_P00507
Aspartate aminotransferase, mitochondrial		PIG		430
Aspartate aminotransferase, mitochondrial		PONAB		430
Aspartate aminotransferase, mitochondrial	)	MOUSE		430
Aspartate aminotransferase, mitochondrial		MACFA		430
+137

Protein	Similar proteins		Species	Score	Length	Source
P12345	Aspartate aminotransferase, mitochondrial		RAT		430	UniRef50_P00507
Aspartate aminotransferase, mitochondrial	)	MOUSE		430
Aspartate aminotransferase, mitochondrial		PIG		430
Aspartate aminotransferase, mitochondrial	)	HUMAN		430
Aspartate aminotransferase, mitochondrial		MACFA		430
+207

Entry informationⁱ

Entry nameⁱ	AATM_RABIT
Accessionⁱ	P12345Primary (citable) accession number: P12345 Secondary accession number(s): G1SKL2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
	Last sequence update:	September 18, 2013
	Last modified:	September 12, 2018
	This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P12345 (AATM_RABIT)

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Aspartate aminotransferase, mitochondrial

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Plasma membrane

Mitochondrion

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni