UniProtKB - P12344 (AATM_BOVIN)
Protein
Aspartate aminotransferase, mitochondrial
Gene
GOT2
Organism
Bos taurus (Bovine)
Status
Functioni
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.By similarity
Miscellaneous
In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.
Catalytic activityi
- EC:2.6.1.1
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 65 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 162 | SubstrateBy similarity | 1 | |
Binding sitei | 215 | SubstrateBy similarity | 1 | |
Binding sitei | 407 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- L-aspartate:2-oxoglutarate aminotransferase activity Source: UniProtKB
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- 2-oxoglutarate metabolic process Source: UniProtKB
- aspartate biosynthetic process Source: Ensembl
- aspartate catabolic process Source: GO_Central
- aspartate metabolic process Source: UniProtKB
- fatty acid transport Source: Ensembl
- glutamate catabolic process to 2-oxoglutarate Source: Ensembl
- glutamate catabolic process to aspartate Source: Ensembl
- glutamate metabolic process Source: UniProtKB
- oxaloacetate metabolic process Source: Ensembl
- response to ethanol Source: Ensembl
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Lipid transport, Transport |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
Reactomei | R-BTA-389661, Glyoxylate metabolism and glycine degradation R-BTA-70263, Gluconeogenesis R-BTA-8963693, Aspartate and asparagine metabolism R-BTA-8964539, Glutamate and glutamine metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate aminotransferase, mitochondrial (EC:2.6.1.1)Short name: mAspAT Alternative name(s): Fatty acid-binding protein Short name: FABP-1 Glutamate oxaloacetate transaminase 2 Kynurenine aminotransferase 4 Kynurenine aminotransferase IV Kynurenine--oxoglutarate transaminase 4 Kynurenine--oxoglutarate transaminase IV Plasma membrane-associated fatty acid-binding protein Short name: FABPpm Transaminase A |
Gene namesi | Name:GOT2 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:29503, GOT2 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Mitochondrion
Mitochondrion
- mitochondrial inner membrane Source: AgBase
- mitochondrial matrix Source: UniProtKB-SubCell
- mitochondrion Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cell surface Source: AgBase
Keywords - Cellular componenti
Cell membrane, Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 29 | Mitochondrion1 PublicationAdd BLAST | 29 | |
ChainiPRO_0000001214 | 30 – 430 | Aspartate aminotransferase, mitochondrialAdd BLAST | 401 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 48 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 59 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 73 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 73 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 82 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 90 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 90 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 96 | 3'-nitrotyrosine; alternateBy similarity | 1 | |
Modified residuei | 96 | Phosphotyrosine; alternateBy similarity | 1 | |
Modified residuei | 107 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 107 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 122 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 122 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 143 | PhosphoserineBy similarity | 1 | |
Modified residuei | 159 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 159 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 185 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 185 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 227 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 234 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 279 | N6-(pyridoxal phosphate)lysine; alternate | 1 | |
Modified residuei | 279 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 296 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 296 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 302 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 309 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 309 | N6-succinyllysine; alternate1 Publication | 1 | |
Modified residuei | 313 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 338 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 338 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 345 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 363 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 363 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 364 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 387 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 396 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 396 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 404 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 404 | N6-succinyllysine; alternateBy similarity | 1 |
Keywords - PTMi
Acetylation, Methylation, Nitration, PhosphoproteinProteomic databases
PaxDbi | P12344 |
PeptideAtlasi | P12344 |
PRIDEi | P12344 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000007172, Expressed in skeletal muscle tissue and 19 other tissues |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
IntActi | P12344, 1 interactor |
STRINGi | 9913.ENSBTAP00000009440 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1411, Eukaryota |
GeneTreei | ENSGT00950000183082 |
HOGENOMi | CLU_032440_1_2_1 |
InParanoidi | P12344 |
OMAi | VGACTIV |
OrthoDBi | 1104596at2759 |
TreeFami | TF300641 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P12344-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALLHSGRFL SGVAAAFHPG LAAAASARAS SWWAHVEMGP PDPILGVTEA
60 70 80 90 100
FKRDTNSKKM NLGVGAYRDD NGKPYVLPSV RKAEAQIAAK NLDKEYLPIA
110 120 130 140 150
GLAEFCKASA ELALGENNEV LKSGRYVTVQ TISGTGALRI GASFLQRFFK
160 170 180 190 200
FSRDVFLPKP TWGNHTPIFR DAGMQLQSYR YYDPKTCGFD FTGAIEDISK
210 220 230 240 250
IPAQSVILLH ACAHNPTGVD PRPEQWKEMA TVVKKNNLFA FFDMAYQGFA
260 270 280 290 300
SGDGNKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTVVCKDAEE
310 320 330 340 350
AKRVESQLKI LIRPMYSNPP INGARIASTI LTSPDLRKQW LHEVKGMADR
360 370 380 390 400
IISMRTQLVS NLKKEGSSHN WQHIIDQIGM FCYTGLKPEQ VERLTKEFSI
410 420 430
YMTKDGRISV AGVTSGNVAY LAHAIHQVTK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z25466 mRNA Translation: CAA80960.1 BT030486 mRNA Translation: ABQ12926.1 BC102303 mRNA Translation: AAI02304.1 |
PIRi | S35960 |
RefSeqi | NP_777231.1, NM_174806.2 |
Genome annotation databases
Ensembli | ENSBTAT00000009440; ENSBTAP00000009440; ENSBTAG00000007172 |
GeneIDi | 286886 |
KEGGi | bta:286886 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z25466 mRNA Translation: CAA80960.1 BT030486 mRNA Translation: ABQ12926.1 BC102303 mRNA Translation: AAI02304.1 |
PIRi | S35960 |
RefSeqi | NP_777231.1, NM_174806.2 |
3D structure databases
SMRi | P12344 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P12344, 1 interactor |
STRINGi | 9913.ENSBTAP00000009440 |
Proteomic databases
PaxDbi | P12344 |
PeptideAtlasi | P12344 |
PRIDEi | P12344 |
Genome annotation databases
Ensembli | ENSBTAT00000009440; ENSBTAP00000009440; ENSBTAG00000007172 |
GeneIDi | 286886 |
KEGGi | bta:286886 |
Organism-specific databases
CTDi | 2806 |
VGNCi | VGNC:29503, GOT2 |
Phylogenomic databases
eggNOGi | KOG1411, Eukaryota |
GeneTreei | ENSGT00950000183082 |
HOGENOMi | CLU_032440_1_2_1 |
InParanoidi | P12344 |
OMAi | VGACTIV |
OrthoDBi | 1104596at2759 |
TreeFami | TF300641 |
Enzyme and pathway databases
Reactomei | R-BTA-389661, Glyoxylate metabolism and glycine degradation R-BTA-70263, Gluconeogenesis R-BTA-8963693, Aspartate and asparagine metabolism R-BTA-8964539, Glutamate and glutamine metabolism |
Gene expression databases
Bgeei | ENSBTAG00000007172, Expressed in skeletal muscle tissue and 19 other tissues |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AATM_BOVIN | |
Accessioni | P12344Primary (citable) accession number: P12344 Secondary accession number(s): A5D967, Q3T0Q1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families