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Protein

Uracil-DNA glycosylase

Gene

ung

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.

Catalytic activityi

Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei64Proton acceptor1

GO - Molecular functioni

  • uracil DNA N-glycosylase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciEcoCyc:EG11058-MONOMER
MetaCyc:EG11058-MONOMER
BRENDAi3.2.2.27 2026
SABIO-RKiP12295

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil-DNA glycosylase (EC:3.2.2.27)
Short name:
UDG
Gene namesi
Name:ung
Ordered Locus Names:b2580, JW2564
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11058 ung

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001760912 – 229Uracil-DNA glycosylaseAdd BLAST228

Proteomic databases

PaxDbiP12295
PRIDEiP12295

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4263474, 132 interactors
DIPiDIP-11092N
IntActiP12295, 10 interactors
STRINGi316385.ECDH10B_2748

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 11Combined sources5
Helixi14 – 16Combined sources3
Helixi18 – 33Combined sources16
Beta strandi37 – 39Combined sources3
Helixi41 – 43Combined sources3
Helixi46 – 50Combined sources5
Helixi53 – 55Combined sources3
Beta strandi58 – 64Combined sources7
Turni69 – 71Combined sources3
Beta strandi74 – 77Combined sources4
Beta strandi81 – 83Combined sources3
Helixi87 – 99Combined sources13
Helixi112 – 115Combined sources4
Turni116 – 118Combined sources3
Beta strandi119 – 125Combined sources7
Turni133 – 138Combined sources6
Helixi141 – 155Combined sources15
Beta strandi160 – 165Combined sources6
Helixi166 – 171Combined sources6
Turni172 – 174Combined sources3
Turni177 – 179Combined sources3
Beta strandi180 – 185Combined sources6
Turni190 – 199Combined sources10
Helixi202 – 212Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EUGX-ray1.60A1-229[»]
1EUIX-ray3.20A/B2-229[»]
1FLZX-ray2.30A2-229[»]
1LQGX-ray2.90A/B2-229[»]
1LQJX-ray3.35A/B/C/D2-229[»]
1LQMX-ray3.20A/C/E/G2-229[»]
1UUGX-ray2.40A/C1-229[»]
2EUGX-ray1.50A1-229[»]
2UUGX-ray2.60A/B1-229[»]
3EUGX-ray1.43A1-229[»]
3UF7X-ray1.20A1-229[»]
4EUGX-ray1.40A1-229[»]
5EUGX-ray1.60A1-229[»]
ProteinModelPortaliP12295
SMRiP12295
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12295

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D5S Bacteria
COG0692 LUCA
HOGENOMiHOG000229528
InParanoidiP12295
KOiK03648
OMAiQANGLCF
PhylomeDBiP12295

Family and domain databases

CDDicd10027 UDG_F1, 1 hit
Gene3Di3.40.470.10, 1 hit
HAMAPiMF_00148 UDG, 1 hit
InterProiView protein in InterPro
IPR002043 UDG_fam1
IPR018085 Ura-DNA_Glyclase_AS
IPR005122 Uracil-DNA_glycosylase-like
IPR036895 Uracil-DNA_glycosylase-like_sf
PANTHERiPTHR11264 PTHR11264, 1 hit
PfamiView protein in Pfam
PF03167 UDG, 1 hit
SMARTiView protein in SMART
SM00986 UDG, 1 hit
SUPFAMiSSF52141 SSF52141, 1 hit
TIGRFAMsiTIGR00628 ung, 1 hit
PROSITEiView protein in PROSITE
PS00130 U_DNA_GLYCOSYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANELTWHDV LAEEKQQPYF LNTLQTVASE RQSGVTIYPP QKDVFNAFRF
60 70 80 90 100
TELGDVKVVI LGQDPYHGPG QAHGLAFSVR PGIAIPPSLL NMYKELENTI
110 120 130 140 150
PGFTRPNHGY LESWARQGVL LLNTVLTVRA GQAHSHASLG WETFTDKVIS
160 170 180 190 200
LINQHREGVV FLLWGSHAQK KGAIIDKQRH HVLKAPHPSP LSAHRGFFGC
210 220
NHFVLANQWL EQRGETPIDW MPVLPAESE
Length:229
Mass (Da):25,693
Last modified:January 23, 2007 - v2
Checksum:iCD44F1E214FE74ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03725 Genomic DNA Translation: AAA24743.1
D13169 Genomic DNA Translation: BAA02448.1
D64044 Genomic DNA Translation: BAA10923.1
U00096 Genomic DNA Translation: AAC75633.1
AP009048 Genomic DNA Translation: BAA16466.1
PIRiA28175 DGECU
RefSeqiNP_417075.1, NC_000913.3
WP_001262716.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75633; AAC75633; b2580
BAA16466; BAA16466; BAA16466
GeneIDi947067
KEGGiecj:JW2564
eco:b2580
PATRICifig|1411691.4.peg.4154

Similar proteinsi

Entry informationi

Entry nameiUNG_ECOLI
AccessioniPrimary (citable) accession number: P12295
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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