UniProtKB - P12282 (MOEB_ECOLI)
Protein
Molybdopterin-synthase adenylyltransferase
Gene
moeB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein MoaD.2 Publications
Catalytic activityi
- [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H+ = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate1 PublicationEC:2.7.7.801 Publication
Cofactori
Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication
: molybdopterin biosynthesis Pathwayi
This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 41 | ATP; via amide nitrogen | 1 | |
Binding sitei | 62 | ATP | 1 | |
Binding sitei | 86 | ATP | 1 | |
Metal bindingi | 172 | Zinc | 1 | |
Metal bindingi | 175 | Zinc | 1 | |
Metal bindingi | 244 | Zinc | 1 | |
Metal bindingi | 247 | Zinc | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 69 – 73 | ATP | 5 | |
Nucleotide bindingi | 130 – 131 | ATP | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- molybdopterin-synthase adenylyltransferase activity Source: EcoCyc
- nucleotidyltransferase activity Source: GO_Central
- ubiquitin-like modifier activating enzyme activity Source: InterPro
GO - Biological processi
- Mo-molybdopterin cofactor biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Molybdenum cofactor biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10154-MONOMER MetaCyc:EG10154-MONOMER |
UniPathwayi | UPA00344 |
Names & Taxonomyi
Protein namesi | Recommended name: Molybdopterin-synthase adenylyltransferase (EC:2.7.7.80)Alternative name(s): MoaD protein adenylase Molybdopterin-converting factor subunit 1 adenylase Sulfur carrier protein MoaD adenylyltransferase |
Gene namesi | Name:moeB Synonyms:chlN Ordered Locus Names:b0826, JW0810 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 14 | R → A or K: No effect. 1 Publication | 1 | |
Mutagenesisi | 14 | R → A: No activity; when associated with A-73. 1 Publication | 1 | |
Mutagenesisi | 44 | C → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 73 | R → A: No effect. No activity; when associated with A-14. 1 Publication | 1 | |
Mutagenesisi | 73 | R → K: Substantially reduced activity. 1 Publication | 1 | |
Mutagenesisi | 128 | C → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 128 | C → Y: No activity. 1 Publication | 1 | |
Mutagenesisi | 130 | D → A: No activity. 1 Publication | 1 | |
Mutagenesisi | 130 | D → E: Substantially reduced activity. 1 Publication | 1 | |
Mutagenesisi | 142 | C → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 172 | C → A: No zinc bound and no enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 175 | C → A: No zinc bound and no enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 187 | C → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 231 | C → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 244 | C → A: No zinc bound and almost no enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 247 | C → A: No zinc bound and almost no enzyme activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120575 | 1 – 249 | Molybdopterin-synthase adenylyltransferaseAdd BLAST | 249 |
Proteomic databases
jPOSTi | P12282 |
PaxDbi | P12282 |
PRIDEi | P12282 |
Interactioni
Subunit structurei
Homodimer.
Forms a stable heterotetrameric complex of 2 MoeB and 2 MoaD during adenylation of MoaD.
1 PublicationBinary interactionsi
P12282
With | #Exp. | IntAct |
---|---|---|
dnaK [P0A6Y8] | 2 | EBI-554435,EBI-542092 |
Protein-protein interaction databases
BioGRIDi | 4261831, 11 interactors |
ComplexPortali | CPX-1968, Molybdopterin-synthase adenylyltransferase complex |
DIPi | DIP-10241N |
IntActi | P12282, 15 interactors |
STRINGi | 511145.b0826 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P12282 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P12282 |
Family & Domainsi
Sequence similaritiesi
Belongs to the HesA/MoeB/ThiF family.Curated
Phylogenomic databases
eggNOGi | COG0476, Bacteria |
HOGENOMi | CLU_013325_10_3_6 |
InParanoidi | P12282 |
PhylomeDBi | P12282 |
Family and domain databases
InterProi | View protein in InterPro IPR012730, Mopterin_Synthase_Sase_MoeB IPR000594, ThiF_NAD_FAD-bd IPR035985, Ubiquitin-activating_enz |
Pfami | View protein in Pfam PF00899, ThiF, 1 hit |
SUPFAMi | SSF69572, SSF69572, 1 hit |
TIGRFAMsi | TIGR02355, moeB, 1 hit |
i Sequence
Sequence statusi: Complete.
P12282-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAELSDQEML RYNRQIILRG FDFDGQEALK DSRVLIVGLG GLGCAASQYL
60 70 80 90 100
ASAGVGNLTL LDFDTVSLSN LQRQTLHSDA TVGQPKVESA RDALTRINPH
110 120 130 140 150
IAITPVNALL DDAELAALIA EHDLVLDCTD NVAVRNQLNA GCFAAKVPLV
160 170 180 190 200
SGAAIRMEGQ ITVFTYQDGE PCYRCLSRLF GENALTCVEA GVMAPLIGVI
210 220 230 240
GSLQAMEAIK MLAGYGKPAS GKIVMYDAMT CQFREMKLMR NPGCEVCGQ
Mass spectrometryi
Molecular mass is 26563 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21151 Genomic DNA Translation: AAA23580.1 U00096 Genomic DNA Translation: AAC73913.1 AP009048 Genomic DNA Translation: BAA35514.1 |
PIRi | B32352 |
RefSeqi | NP_415347.1, NC_000913.3 WP_000829217.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC73913; AAC73913; b0826 BAA35514; BAA35514; BAA35514 |
GeneIDi | 945452 |
KEGGi | ecj:JW0810 eco:b0826 |
PATRICi | fig|1411691.4.peg.1452 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21151 Genomic DNA Translation: AAA23580.1 U00096 Genomic DNA Translation: AAC73913.1 AP009048 Genomic DNA Translation: BAA35514.1 |
PIRi | B32352 |
RefSeqi | NP_415347.1, NC_000913.3 WP_000829217.1, NZ_SSZK01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JW9 | X-ray | 1.70 | B | 1-249 | [»] | |
1JWA | X-ray | 2.90 | B | 1-249 | [»] | |
1JWB | X-ray | 2.10 | B | 1-249 | [»] | |
SMRi | P12282 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261831, 11 interactors |
ComplexPortali | CPX-1968, Molybdopterin-synthase adenylyltransferase complex |
DIPi | DIP-10241N |
IntActi | P12282, 15 interactors |
STRINGi | 511145.b0826 |
Proteomic databases
jPOSTi | P12282 |
PaxDbi | P12282 |
PRIDEi | P12282 |
Genome annotation databases
EnsemblBacteriai | AAC73913; AAC73913; b0826 BAA35514; BAA35514; BAA35514 |
GeneIDi | 945452 |
KEGGi | ecj:JW0810 eco:b0826 |
PATRICi | fig|1411691.4.peg.1452 |
Organism-specific databases
EchoBASEi | EB0152 |
Phylogenomic databases
eggNOGi | COG0476, Bacteria |
HOGENOMi | CLU_013325_10_3_6 |
InParanoidi | P12282 |
PhylomeDBi | P12282 |
Enzyme and pathway databases
UniPathwayi | UPA00344 |
BioCyci | EcoCyc:EG10154-MONOMER MetaCyc:EG10154-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P12282 |
PROi | PR:P12282 |
Family and domain databases
InterProi | View protein in InterPro IPR012730, Mopterin_Synthase_Sase_MoeB IPR000594, ThiF_NAD_FAD-bd IPR035985, Ubiquitin-activating_enz |
Pfami | View protein in Pfam PF00899, ThiF, 1 hit |
SUPFAMi | SSF69572, SSF69572, 1 hit |
TIGRFAMsi | TIGR02355, moeB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MOEB_ECOLI | |
Accessioni | P12282Primary (citable) accession number: P12282 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | October 1, 1989 | |
Last modified: | December 2, 2020 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families