UniProtKB - P12281 (MOEA_ECOLI)
Protein
Molybdopterin molybdenumtransferase
Gene
moeA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP.1 Publication
Catalytic activityi
- EC:2.10.1.1
Cofactori
Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication
: molybdopterin biosynthesis Pathwayi
This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.
GO - Molecular functioni
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
- molybdopterin adenylyltransferase activity Source: GO_Central
- molybdopterin molybdotransferase activity Source: EcoCyc
GO - Biological processi
- molybdenum incorporation into molybdenum-molybdopterin complex Source: EcoCyc
- molybdopterin cofactor biosynthetic process Source: GO_Central
- Mo-molybdopterin cofactor biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Transferase |
Biological process | Molybdenum cofactor biosynthesis |
Ligand | Magnesium, Metal-binding, Molybdenum |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10153-MONOMER MetaCyc:EG10153-MONOMER |
BRENDAi | 2.10.1.1, 2026 |
UniPathwayi | UPA00344 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:moeA Synonyms:bisB, chlE, narE Ordered Locus Names:b0827, JW0811 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000170989 | 1 – 411 | Molybdopterin molybdenumtransferaseAdd BLAST | 411 |
Proteomic databases
jPOSTi | P12281 |
PaxDbi | P12281 |
PRIDEi | P12281 |
Interactioni
Subunit structurei
Homodimer.
Interacts with MobA, MogA and MobB in vivo.
3 PublicationsBinary interactionsi
Hide detailsP12281
With | #Exp. | IntAct |
---|---|---|
mobA [P32173] | 3 | EBI-554393,EBI-1133881 |
itself | 5 | EBI-554393,EBI-554393 |
pfkA [P0A796] | 2 | EBI-554393,EBI-554405 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259979, 41 interactors 849828, 1 interactor |
DIPi | DIP-10240N |
IntActi | P12281, 8 interactors |
STRINGi | 511145.b0827 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P12281 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P12281 |
Family & Domainsi
Sequence similaritiesi
Belongs to the MoeA family.Curated
Phylogenomic databases
eggNOGi | COG0303, Bacteria |
HOGENOMi | CLU_010186_7_0_6 |
InParanoidi | P12281 |
PhylomeDBi | P12281 |
Family and domain databases
CDDi | cd00887, MoeA, 1 hit |
Gene3Di | 2.40.340.10, 1 hit 3.40.980.10, 1 hit |
InterProi | View protein in InterPro IPR036425, MoaB/Mog-like_dom_sf IPR001453, MoaB/Mog_dom IPR008284, MoCF_biosynth_CS IPR038987, MoeA-like IPR005111, MoeA_C_domain_IV IPR036688, MoeA_C_domain_IV_sf IPR005110, MoeA_linker/N IPR036135, MoeA_linker/N_sf |
PANTHERi | PTHR10192, PTHR10192, 1 hit |
Pfami | View protein in Pfam PF00994, MoCF_biosynth, 1 hit PF03454, MoeA_C, 1 hit PF03453, MoeA_N, 1 hit |
SMARTi | View protein in SMART SM00852, MoCF_biosynth, 1 hit |
SUPFAMi | SSF53218, SSF53218, 1 hit SSF63867, SSF63867, 1 hit SSF63882, SSF63882, 1 hit |
TIGRFAMsi | TIGR00177, molyb_syn, 1 hit |
PROSITEi | View protein in PROSITE PS01079, MOCF_BIOSYNTHESIS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P12281-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEFTTGLMSL DTALNEMLSR VTPLTAQETL PLVQCFGRIL ASDVVSPLDV
60 70 80 90 100
PGFDNSAMDG YAVRLADIAS GQPLPVAGKS FAGQPYHGEW PAGTCIRIMT
110 120 130 140 150
GAPVPEGCEA VVMQEQTEQM DNGVRFTAEV RSGQNIRRRG EDISAGAVVF
160 170 180 190 200
PAGTRLTTAE LPVIASLGIA EVPVIRKVRV ALFSTGDELQ LPGQPLGDGQ
210 220 230 240 250
IYDTNRLAVH LMLEQLGCEV INLGIIRDDP HALRAAFIEA DSQADVVISS
260 270 280 290 300
GGVSVGEADY TKTILEELGE IAFWKLAIKP GKPFAFGKLS NSWFCGLPGN
310 320 330 340 350
PVSATLTFYQ LVQPLLAKLS GNTASGLPAR QRVRTASRLK KTPGRLDFQR
360 370 380 390 400
GVLQRNADGE LEVTTTGHQG SHIFSSFSLG NCFIVLERDR GNVEVGEWVE
410
VEPFNALFGG L
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21151 Genomic DNA Translation: AAA23579.1 U00096 Genomic DNA Translation: AAC73914.1 AP009048 Genomic DNA Translation: BAA35515.1 |
PIRi | A32352 |
RefSeqi | NP_415348.1, NC_000913.3 WP_000397340.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC73914; AAC73914; b0827 BAA35515; BAA35515; BAA35515 |
GeneIDi | 945454 |
KEGGi | ecj:JW0811 eco:b0827 |
PATRICi | fig|1411691.4.peg.1451 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21151 Genomic DNA Translation: AAA23579.1 U00096 Genomic DNA Translation: AAC73914.1 AP009048 Genomic DNA Translation: BAA35515.1 |
PIRi | A32352 |
RefSeqi | NP_415348.1, NC_000913.3 WP_000397340.1, NZ_SSZK01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FC5 | X-ray | 2.20 | A/B | 1-411 | [»] | |
1G8L | X-ray | 1.95 | A/B | 1-411 | [»] | |
1G8R | X-ray | 2.65 | A/B | 1-411 | [»] | |
2NQK | X-ray | 2.90 | A/B | 1-411 | [»] | |
2NQM | X-ray | 3.00 | A/B | 1-411 | [»] | |
2NQN | X-ray | 2.20 | A/B | 1-411 | [»] | |
2NQQ | X-ray | 2.40 | A/B/C/D | 1-411 | [»] | |
2NQR | X-ray | 2.20 | A/B | 1-411 | [»] | |
2NQS | X-ray | 2.50 | A/B | 1-411 | [»] | |
2NQU | X-ray | 2.70 | A/B | 1-411 | [»] | |
2NQV | X-ray | 2.82 | A/B | 1-411 | [»] | |
2NRO | X-ray | 2.50 | A/B | 1-411 | [»] | |
2NRP | X-ray | 3.00 | A/B | 1-411 | [»] | |
2NRS | X-ray | 2.80 | A/B | 1-411 | [»] | |
SMRi | P12281 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259979, 41 interactors 849828, 1 interactor |
DIPi | DIP-10240N |
IntActi | P12281, 8 interactors |
STRINGi | 511145.b0827 |
Proteomic databases
jPOSTi | P12281 |
PaxDbi | P12281 |
PRIDEi | P12281 |
Genome annotation databases
EnsemblBacteriai | AAC73914; AAC73914; b0827 BAA35515; BAA35515; BAA35515 |
GeneIDi | 945454 |
KEGGi | ecj:JW0811 eco:b0827 |
PATRICi | fig|1411691.4.peg.1451 |
Organism-specific databases
EchoBASEi | EB0151 |
Phylogenomic databases
eggNOGi | COG0303, Bacteria |
HOGENOMi | CLU_010186_7_0_6 |
InParanoidi | P12281 |
PhylomeDBi | P12281 |
Enzyme and pathway databases
UniPathwayi | UPA00344 |
BioCyci | EcoCyc:EG10153-MONOMER MetaCyc:EG10153-MONOMER |
BRENDAi | 2.10.1.1, 2026 |
Miscellaneous databases
EvolutionaryTracei | P12281 |
PROi | PR:P12281 |
Family and domain databases
CDDi | cd00887, MoeA, 1 hit |
Gene3Di | 2.40.340.10, 1 hit 3.40.980.10, 1 hit |
InterProi | View protein in InterPro IPR036425, MoaB/Mog-like_dom_sf IPR001453, MoaB/Mog_dom IPR008284, MoCF_biosynth_CS IPR038987, MoeA-like IPR005111, MoeA_C_domain_IV IPR036688, MoeA_C_domain_IV_sf IPR005110, MoeA_linker/N IPR036135, MoeA_linker/N_sf |
PANTHERi | PTHR10192, PTHR10192, 1 hit |
Pfami | View protein in Pfam PF00994, MoCF_biosynth, 1 hit PF03454, MoeA_C, 1 hit PF03453, MoeA_N, 1 hit |
SMARTi | View protein in SMART SM00852, MoCF_biosynth, 1 hit |
SUPFAMi | SSF53218, SSF53218, 1 hit SSF63867, SSF63867, 1 hit SSF63882, SSF63882, 1 hit |
TIGRFAMsi | TIGR00177, molyb_syn, 1 hit |
PROSITEi | View protein in PROSITE PS01079, MOCF_BIOSYNTHESIS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MOEA_ECOLI | |
Accessioni | P12281Primary (citable) accession number: P12281 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | October 1, 1989 | |
Last modified: | December 2, 2020 | |
This is version 181 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families