UniProtKB - P12277 (KCRB_HUMAN)
Protein
Creatine kinase B-type
Gene
CKB
Organism
Homo sapiens (Human)
Status
Functioni
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Catalytic activityi
- EC:2.7.3.2PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 72 | Substrate; via amide nitrogen1 Publication | 1 | |
Binding sitei | 130 | ATP1 Publication | 1 | |
Binding sitei | 132 | ATP1 Publication | 1 | |
Binding sitei | 191 | ATP1 Publication | 1 | |
Binding sitei | 232 | Substrate1 Publication | 1 | |
Binding sitei | 236 | ATP1 Publication | 1 | |
Binding sitei | 285 | Substrate1 Publication | 1 | |
Binding sitei | 292 | ATP1 Publication | 1 | |
Binding sitei | 320 | ATP1 Publication | 1 | |
Binding sitei | 335 | ATP1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 128 – 132 | ATP1 Publication | 5 | |
Nucleotide bindingi | 320 – 325 | ATP1 Publication | 6 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- creatine kinase activity Source: AgBase
- kinase activity Source: GO_Central
- ubiquitin protein ligase binding Source: ParkinsonsUK-UCL
GO - Biological processi
- cellular chloride ion homeostasis Source: Ensembl
- cerebellum development Source: Ensembl
- creatine metabolic process Source: Reactome
- phosphocreatine biosynthetic process Source: GO_Central
- substantia nigra development Source: UniProtKB
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:HS09344-MONOMER |
BRENDAi | 2.7.3.2, 2681 |
PathwayCommonsi | P12277 |
Reactomei | R-HSA-71288, Creatine metabolism |
SABIO-RKi | P12277 |
Names & Taxonomyi
Protein namesi | Recommended name: Creatine kinase B-typeCurated (EC:2.7.3.2)Alternative name(s): Brain creatine kinase1 Publication Short name: B-CK Creatine kinase B chain1 Publication Creatine phosphokinase B-type Short name: CPK-B |
Gene namesi | Name:CKB Synonyms:CKBB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1991, CKB |
MIMi | 123280, gene |
neXtProti | NX_P12277 |
VEuPathDBi | HostDB:ENSG00000166165.12 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular space Source: AgBase
Nucleus
- nucleus Source: Ensembl
Other locations
- dendrite Source: Ensembl
- neuronal cell body Source: Ensembl
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 283 | C → S or Y: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 292 | R → H, L or Q: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 292 | R → K: 42% of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 340 | D → E: No change in activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 1152 |
OpenTargetsi | ENSG00000166165 |
PharmGKBi | PA26528 |
Miscellaneous databases
Pharosi | P12277, Tbio |
Chemistry databases
ChEMBLi | CHEMBL6049 |
DrugBanki | DB00787, Acyclovir DB00148, Creatine DB13749, Magnesium gluconate DB13191, Phosphocreatine DB00300, Tenofovir disoproxil |
Genetic variation databases
BioMutai | CKB |
DMDMi | 125294 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000211966 | 2 – 381 | Creatine kinase B-typeAdd BLAST | 380 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 4 | PhosphoserineCombined sources | 1 | |
Modified residuei | 35 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 125 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 199 | PhosphoserineCombined sources | 1 | |
Modified residuei | 269 | 3'-nitrotyrosineBy similarity | 1 | |
Modified residuei | 309 | PhosphoserineBy similarity | 1 | |
Modified residuei | 322 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Nitration, PhosphoproteinProteomic databases
CPTACi | CPTAC-476 CPTAC-477 |
EPDi | P12277 |
jPOSTi | P12277 |
MassIVEi | P12277 |
PaxDbi | P12277 |
PeptideAtlasi | P12277 |
PRIDEi | P12277 |
ProteomicsDBi | 52846 |
TopDownProteomicsi | P12277 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00022977 P12277 |
UCD-2DPAGEi | P12277 |
PTM databases
CarbonylDBi | P12277 |
iPTMneti | P12277 |
PhosphoSitePlusi | P12277 |
SwissPalmi | P12277 |
Expressioni
Gene expression databases
Bgeei | ENSG00000166165, Expressed in ventricular zone and 130 other tissues |
ExpressionAtlasi | P12277, baseline and differential |
Genevisiblei | P12277, HS |
Organism-specific databases
HPAi | ENSG00000166165, Tissue enhanced (brain) |
Interactioni
Subunit structurei
Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.
1 PublicationBinary interactionsi
Hide detailsP12277
With | #Exp. | IntAct |
---|---|---|
ASB9 [Q96DX5] | 10 | EBI-357706,EBI-745641 |
Non-structural protein 4A (PRO_0000278738) from Hepatitis C virus genotype 1b (isolate HC-J1). | 3 | EBI-357706,EBI-9081620 |
GO - Molecular functioni
- ubiquitin protein ligase binding Source: ParkinsonsUK-UCL
Protein-protein interaction databases
BioGRIDi | 107572, 122 interactors |
CORUMi | P12277 |
DIPi | DIP-52968N |
IntActi | P12277, 70 interactors |
MINTi | P12277 |
STRINGi | 9606.ENSP00000299198 |
Miscellaneous databases
RNActi | P12277, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P12277 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P12277 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 98 | Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST | 88 | |
Domaini | 125 – 367 | Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 243 |
Sequence similaritiesi
Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG3581, Eukaryota |
GeneTreei | ENSGT01010000222526 |
HOGENOMi | CLU_019868_4_2_1 |
InParanoidi | P12277 |
OMAi | RGHEFMW |
PhylomeDBi | P12277 |
TreeFami | TF314214 |
Family and domain databases
Gene3Di | 1.10.135.10, 1 hit |
InterProi | View protein in InterPro IPR000749, ATP-guanido_PTrfase IPR022415, ATP-guanido_PTrfase_AS IPR022414, ATP-guanido_PTrfase_cat IPR022413, ATP-guanido_PTrfase_N IPR036802, ATP-guanido_PTrfase_N_sf IPR014746, Gln_synth/guanido_kin_cat_dom |
PANTHERi | PTHR11547, PTHR11547, 2 hits |
Pfami | View protein in Pfam PF00217, ATP-gua_Ptrans, 1 hit PF02807, ATP-gua_PtransN, 1 hit |
SUPFAMi | SSF48034, SSF48034, 1 hit SSF55931, SSF55931, 1 hit |
PROSITEi | View protein in PROSITE PS00112, PHOSPHAGEN_KINASE, 1 hit PS51510, PHOSPHAGEN_KINASE_C, 1 hit PS51509, PHOSPHAGEN_KINASE_N, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
P12277-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPFSNSHNAL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG
60 70 80 90 100
FTLDDVIQTG VDNPGHPYIM TVGCVAGDEE SYEVFKDLFD PIIEDRHGGY
110 120 130 140 150
KPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE
160 170 180 190 200
RRAIEKLAVE ALSSLDGDLA GRYYALKSMT EAEQQQLIDD HFLFDKPVSP
210 220 230 240 250
LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM QKGGNMKEVF
260 270 280 290 300
TRFCTGLTQI ETLFKSKDYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP
310 320 330 340 350
NLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV
360 370 380
QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V4N7 | G3V4N7_HUMAN | Creatine kinase | CKB | 217 | Annotation score: | ||
H0YJG0 | H0YJG0_HUMAN | Creatine kinase | CKB | 179 | Annotation score: | ||
G3V461 | G3V461_HUMAN | Creatine kinase | CKB | 128 | Annotation score: | ||
H0YJK0 | H0YJK0_HUMAN | Creatine kinase | CKB | 90 | Annotation score: | ||
H0YJJ7 | H0YJJ7_HUMAN | Creatine kinase B-type | CKB | 20 | Annotation score: | ||
G3V2I1 | G3V2I1_HUMAN | Creatine kinase B-type | CKB | 58 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 41 – 42 | EL → DV in AAA76851 (PubMed:3034271).Curated | 2 | |
Sequence conflicti | 78 | D → G in AAA76850 (PubMed:2883200).Curated | 1 | |
Sequence conflicti | 78 | D → G in AAA52024 (PubMed:2828370).Curated | 1 | |
Sequence conflicti | 98 – 99 | GG → RR in AAA76851 (PubMed:3034271).Curated | 2 | |
Sequence conflicti | 105 – 106 | EH → DD in AAA76851 (PubMed:3034271).Curated | 2 | |
Sequence conflicti | 130 | R → G in AAA76850 (PubMed:2883200).Curated | 1 | |
Sequence conflicti | 132 | R → A in AAA76851 (PubMed:3034271).Curated | 1 | |
Sequence conflicti | 144 | P → Q in CAG47064 (Ref. 6) Curated | 1 | |
Sequence conflicti | 203 | L → S in AAC31758 (PubMed:3692484).Curated | 1 | |
Sequence conflicti | 215 – 216 | RG → AR in AAA76851 (PubMed:3034271).Curated | 2 | |
Sequence conflicti | 296 | H → D in AAA76851 (PubMed:3034271).Curated | 1 | |
Sequence conflicti | 358 | K → R in BAG35211 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_025838 | 177 | K → R1 PublicationCorresponds to variant dbSNP:rs36002620Ensembl. | 1 | |
Natural variantiVAR_025839 | 309 | S → L1 PublicationCorresponds to variant dbSNP:rs35156510Ensembl. | 1 | |
Natural variantiVAR_049674 | 360 | L → F. Corresponds to variant dbSNP:rs12505Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16451 mRNA Translation: AAA76851.1 M21243 , M21237, M21238, M21239, M21240, M21241, M21242 Genomic DNA Translation: AAC31758.1 L47647 mRNA Translation: AAA76852.1 M16364 mRNA Translation: AAA76850.1 X15334 Genomic DNA Translation: CAA33389.1 AK290101 mRNA Translation: BAF82790.1 AK312282 mRNA Translation: BAG35211.1 CR542268 mRNA Translation: CAG47064.1 DQ333313 Genomic DNA Translation: ABC67465.1 CH471061 Genomic DNA Translation: EAW81822.1 BC001190 mRNA Translation: AAH01190.1 BC004914 mRNA Translation: AAH04914.1 BC008323 mRNA Translation: AAH08323.1 BC010002 mRNA Translation: AAH10002.1 BC019259 mRNA Translation: AAH19259.1 BC019281 mRNA Translation: AAH19281.1 M22356, M22355 Genomic DNA Translation: AAA52024.1 |
CCDSi | CCDS9981.1 |
PIRi | S15935, KIHUCB |
RefSeqi | NP_001814.2, NM_001823.4 |
Genome annotation databases
Ensembli | ENST00000348956; ENSP00000299198; ENSG00000166165 |
GeneIDi | 1152 |
KEGGi | hsa:1152 |
Similar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Wikipedia Creatine kinase entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16451 mRNA Translation: AAA76851.1 M21243 , M21237, M21238, M21239, M21240, M21241, M21242 Genomic DNA Translation: AAC31758.1 L47647 mRNA Translation: AAA76852.1 M16364 mRNA Translation: AAA76850.1 X15334 Genomic DNA Translation: CAA33389.1 AK290101 mRNA Translation: BAF82790.1 AK312282 mRNA Translation: BAG35211.1 CR542268 mRNA Translation: CAG47064.1 DQ333313 Genomic DNA Translation: ABC67465.1 CH471061 Genomic DNA Translation: EAW81822.1 BC001190 mRNA Translation: AAH01190.1 BC004914 mRNA Translation: AAH04914.1 BC008323 mRNA Translation: AAH08323.1 BC010002 mRNA Translation: AAH10002.1 BC019259 mRNA Translation: AAH19259.1 BC019281 mRNA Translation: AAH19281.1 M22356, M22355 Genomic DNA Translation: AAA52024.1 |
CCDSi | CCDS9981.1 |
PIRi | S15935, KIHUCB |
RefSeqi | NP_001814.2, NM_001823.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3B6R | X-ray | 2.00 | A/B | 1-381 | [»] | |
3DRB | X-ray | 2.00 | A/B | 1-381 | [»] | |
3DRE | X-ray | 2.20 | A/B | 1-381 | [»] | |
6V9H | electron microscopy | 4.10 | A/B | 1-381 | [»] | |
SMRi | P12277 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107572, 122 interactors |
CORUMi | P12277 |
DIPi | DIP-52968N |
IntActi | P12277, 70 interactors |
MINTi | P12277 |
STRINGi | 9606.ENSP00000299198 |
Chemistry databases
ChEMBLi | CHEMBL6049 |
DrugBanki | DB00787, Acyclovir DB00148, Creatine DB13749, Magnesium gluconate DB13191, Phosphocreatine DB00300, Tenofovir disoproxil |
PTM databases
CarbonylDBi | P12277 |
iPTMneti | P12277 |
PhosphoSitePlusi | P12277 |
SwissPalmi | P12277 |
Genetic variation databases
BioMutai | CKB |
DMDMi | 125294 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00022977 P12277 |
UCD-2DPAGEi | P12277 |
Proteomic databases
CPTACi | CPTAC-476 CPTAC-477 |
EPDi | P12277 |
jPOSTi | P12277 |
MassIVEi | P12277 |
PaxDbi | P12277 |
PeptideAtlasi | P12277 |
PRIDEi | P12277 |
ProteomicsDBi | 52846 |
TopDownProteomicsi | P12277 |
Protocols and materials databases
Antibodypediai | 28, 811 antibodies |
CPTCi | P12277, 3 antibodies |
DNASUi | 1152 |
Genome annotation databases
Ensembli | ENST00000348956; ENSP00000299198; ENSG00000166165 |
GeneIDi | 1152 |
KEGGi | hsa:1152 |
Organism-specific databases
CTDi | 1152 |
DisGeNETi | 1152 |
GeneCardsi | CKB |
HGNCi | HGNC:1991, CKB |
HPAi | ENSG00000166165, Tissue enhanced (brain) |
MIMi | 123280, gene |
neXtProti | NX_P12277 |
OpenTargetsi | ENSG00000166165 |
PharmGKBi | PA26528 |
VEuPathDBi | HostDB:ENSG00000166165.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3581, Eukaryota |
GeneTreei | ENSGT01010000222526 |
HOGENOMi | CLU_019868_4_2_1 |
InParanoidi | P12277 |
OMAi | RGHEFMW |
PhylomeDBi | P12277 |
TreeFami | TF314214 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS09344-MONOMER |
BRENDAi | 2.7.3.2, 2681 |
PathwayCommonsi | P12277 |
Reactomei | R-HSA-71288, Creatine metabolism |
SABIO-RKi | P12277 |
Miscellaneous databases
BioGRID-ORCSi | 1152, 1 hit in 871 CRISPR screens |
ChiTaRSi | CKB, human |
EvolutionaryTracei | P12277 |
GeneWikii | CKB_(gene) |
GenomeRNAii | 1152 |
Pharosi | P12277, Tbio |
PROi | PR:P12277 |
RNActi | P12277, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000166165, Expressed in ventricular zone and 130 other tissues |
ExpressionAtlasi | P12277, baseline and differential |
Genevisiblei | P12277, HS |
Family and domain databases
Gene3Di | 1.10.135.10, 1 hit |
InterProi | View protein in InterPro IPR000749, ATP-guanido_PTrfase IPR022415, ATP-guanido_PTrfase_AS IPR022414, ATP-guanido_PTrfase_cat IPR022413, ATP-guanido_PTrfase_N IPR036802, ATP-guanido_PTrfase_N_sf IPR014746, Gln_synth/guanido_kin_cat_dom |
PANTHERi | PTHR11547, PTHR11547, 2 hits |
Pfami | View protein in Pfam PF00217, ATP-gua_Ptrans, 1 hit PF02807, ATP-gua_PtransN, 1 hit |
SUPFAMi | SSF48034, SSF48034, 1 hit SSF55931, SSF55931, 1 hit |
PROSITEi | View protein in PROSITE PS00112, PHOSPHAGEN_KINASE, 1 hit PS51510, PHOSPHAGEN_KINASE_C, 1 hit PS51509, PHOSPHAGEN_KINASE_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCRB_HUMAN | |
Accessioni | P12277Primary (citable) accession number: P12277 Secondary accession number(s): A8K236 Q9UC66 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | October 1, 1989 | |
Last modified: | February 10, 2021 | |
This is version 214 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families