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Protein

Beta-glucuronidase

Gene

Gusb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in the degradation of dermatan and keratan sulfates.

Catalytic activityi

A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.

Activity regulationi

Inhibited by L-aspartic acid.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei447Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiR-MMU-2024096 HS-GAG degradation
R-MMU-2160916 Hyaluronan uptake and degradation
R-MMU-6798695 Neutrophil degranulation

Protein family/group databases

CAZyiGH2 Glycoside Hydrolase Family 2

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucuronidase (EC:3.2.1.31)
Gene namesi
Name:Gusb
Synonyms:Gus, Gus-s
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:95872 Gusb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000001216223 – 648Beta-glucuronidaseAdd BLAST626

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi172N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi416N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi627N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiP12265
PaxDbiP12265
PeptideAtlasiP12265
PRIDEiP12265

PTM databases

iPTMnetiP12265
PhosphoSitePlusiP12265
SwissPalmiP12265

Expressioni

Gene expression databases

BgeeiENSMUSG00000025534 Expressed in 286 organ(s), highest expression level in ileum
CleanExiMM_GUSB
ExpressionAtlasiP12265 baseline and differential
GenevisibleiP12265 MM

Interactioni

Subunit structurei

Homotetramer.

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP12265
IntActiP12265, 2 interactors
MINTiP12265
STRINGi10090.ENSMUSP00000026613

Structurei

3D structure databases

ProteinModelPortaliP12265
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2024 Eukaryota
COG3250 LUCA
GeneTreeiENSGT00390000001752
HOGENOMiHOG000120896
HOVERGENiHBG004843
InParanoidiP12265
KOiK01195
OMAiQRWTQDL
OrthoDBiEOG091G02MO
TreeFamiTF300685

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR036156 Beta-gal/glucu_dom_sf
IPR008979 Galactose-bd-like_sf
IPR006101 Glyco_hydro_2
IPR023232 Glyco_hydro_2_AS
IPR006103 Glyco_hydro_2_cat
IPR023230 Glyco_hydro_2_CS
IPR006102 Glyco_hydro_2_Ig-like
IPR006104 Glyco_hydro_2_N
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00703 Glyco_hydro_2, 1 hit
PF02836 Glyco_hydro_2_C, 1 hit
PF02837 Glyco_hydro_2_N, 1 hit
PRINTSiPR00132 GLHYDRLASE2
SUPFAMiSSF49303 SSF49303, 1 hit
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00719 GLYCOSYL_HYDROL_F2_1, 1 hit
PS00608 GLYCOSYL_HYDROL_F2_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P12265-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLKWSACWV ALGQLLCSCA LALKGGMLFP KESPSRELKA LDGLWHFRAD
60 70 80 90 100
LSNNRLQGFE QQWYRQPLRE SGPVLDMPVP SSFNDITQEA ALRDFIGWVW
110 120 130 140 150
YEREAILPRR WTQDTDMRVV LRINSAHYYA VVWVNGIHVV EHEGGHLPFE
160 170 180 190 200
ADISKLVQSG PLTTCRITIA INNTLTPHTL PPGTIVYKTD TSMYPKGYFV
210 220 230 240 250
QDTSFDFFNY AGLHRSVVLY TTPTTYIDDI TVITNVEQDI GLVTYWISVQ
260 270 280 290 300
GSEHFQLEVQ LLDEGGKVVA HGTGNQGQLQ VPSANLWWPY LMHEHPAYMY
310 320 330 340 350
SLEVKVTTTE SVTDYYTLPI GIRTVAVTKS KFLINGKPFY FQGVNKHEDS
360 370 380 390 400
DIRGKGFDWP LLVKDFNLLR WLGANSFRTS HYPYSEEVLQ LCDRYGIVVI
410 420 430 440 450
DECPGVGIVL PQSFGNESLR HHLEVMEELV RRDKNHPAVV MWSVANEPSS
460 470 480 490 500
ALKPAAYYFK TLITHTKALD LTRPVTFVSN AKYDADLGAP YVDVICVNSY
510 520 530 540 550
FSWYHDYGHL EVIQPQLNSQ FENWYKTHQK PIIQSEYGAD AIPGIHEDPP
560 570 580 590 600
RMFSEEYQKA VLENYHSVLD QKRKEYVVGE LIWNFADFMT NQSPLRVIGN
610 620 630 640
KKGIFTRQRQ PKTSAFILRE RYWRIANETG GHGSGPRTQC FGSRPFTF
Length:648
Mass (Da):74,195
Last modified:October 3, 2012 - v2
Checksum:iCCD8F84C3CD6C498
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q99KJ6Q99KJ6_MOUSE
Beta-glucuronidase
Gusb
250Annotation score:
D3YY48D3YY48_MOUSE
Beta-glucuronidase
Gusb
144Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti265G → D in AAA37696 (PubMed:3397060).Curated1
Sequence conflicti265G → D in AAA98623 (PubMed:3196706).Curated1
Sequence conflicti265G → D in AAA63309 (PubMed:2779578).Curated1
Sequence conflicti265G → D in AAA37697 (PubMed:2835664).Curated1
Sequence conflicti320I → V in AAA37696 (PubMed:3397060).Curated1
Sequence conflicti320I → V in AAA98623 (PubMed:3196706).Curated1
Sequence conflicti320I → V in AAA37697 (PubMed:2835664).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti87T → I in strain: C3H/HeJ. 1
Natural varianti233I → T in allele GUS-SA. 1
Natural varianti428E → K in allele GUS-SA. 1
Natural varianti616F → L in allele GUS-SA. 1
Natural varianti642G → R in allele W26; reduced retention in the endoplasmic reticulum. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03047 mRNA Translation: AAA37696.1
J02836 Genomic DNA Translation: AAA98623.1
M63836 mRNA Translation: AAA63309.1
M28540 mRNA Translation: AAA63307.1
M28541 mRNA Translation: AAA63308.1
M19279 mRNA Translation: AAA37697.1
AK136519 mRNA Translation: BAE23021.1
AK150048 mRNA Translation: BAE29265.1
AK159526 mRNA Translation: BAE35155.1
AK162436 mRNA Translation: BAE36917.1
AC161345 Genomic DNA No translation available.
CH466529 Genomic DNA Translation: EDL19485.1
BC071226 mRNA Translation: AAH71226.1
CCDSiCCDS19705.1
PIRiA32576
RefSeqiNP_034498.1, NM_010368.1
UniGeneiMm.3317

Genome annotation databases

EnsembliENSMUST00000026613; ENSMUSP00000026613; ENSMUSG00000025534
GeneIDi110006
KEGGimmu:110006
UCSCiuc008ztt.1 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03047 mRNA Translation: AAA37696.1
J02836 Genomic DNA Translation: AAA98623.1
M63836 mRNA Translation: AAA63309.1
M28540 mRNA Translation: AAA63307.1
M28541 mRNA Translation: AAA63308.1
M19279 mRNA Translation: AAA37697.1
AK136519 mRNA Translation: BAE23021.1
AK150048 mRNA Translation: BAE29265.1
AK159526 mRNA Translation: BAE35155.1
AK162436 mRNA Translation: BAE36917.1
AC161345 Genomic DNA No translation available.
CH466529 Genomic DNA Translation: EDL19485.1
BC071226 mRNA Translation: AAH71226.1
CCDSiCCDS19705.1
PIRiA32576
RefSeqiNP_034498.1, NM_010368.1
UniGeneiMm.3317

3D structure databases

ProteinModelPortaliP12265
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiP12265
IntActiP12265, 2 interactors
MINTiP12265
STRINGi10090.ENSMUSP00000026613

Protein family/group databases

CAZyiGH2 Glycoside Hydrolase Family 2

PTM databases

iPTMnetiP12265
PhosphoSitePlusiP12265
SwissPalmiP12265

Proteomic databases

EPDiP12265
PaxDbiP12265
PeptideAtlasiP12265
PRIDEiP12265

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026613; ENSMUSP00000026613; ENSMUSG00000025534
GeneIDi110006
KEGGimmu:110006
UCSCiuc008ztt.1 mouse

Organism-specific databases

CTDi2990
MGIiMGI:95872 Gusb

Phylogenomic databases

eggNOGiKOG2024 Eukaryota
COG3250 LUCA
GeneTreeiENSGT00390000001752
HOGENOMiHOG000120896
HOVERGENiHBG004843
InParanoidiP12265
KOiK01195
OMAiQRWTQDL
OrthoDBiEOG091G02MO
TreeFamiTF300685

Enzyme and pathway databases

ReactomeiR-MMU-2024096 HS-GAG degradation
R-MMU-2160916 Hyaluronan uptake and degradation
R-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiGusb mouse
PROiPR:P12265
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025534 Expressed in 286 organ(s), highest expression level in ileum
CleanExiMM_GUSB
ExpressionAtlasiP12265 baseline and differential
GenevisibleiP12265 MM

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR036156 Beta-gal/glucu_dom_sf
IPR008979 Galactose-bd-like_sf
IPR006101 Glyco_hydro_2
IPR023232 Glyco_hydro_2_AS
IPR006103 Glyco_hydro_2_cat
IPR023230 Glyco_hydro_2_CS
IPR006102 Glyco_hydro_2_Ig-like
IPR006104 Glyco_hydro_2_N
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00703 Glyco_hydro_2, 1 hit
PF02836 Glyco_hydro_2_C, 1 hit
PF02837 Glyco_hydro_2_N, 1 hit
PRINTSiPR00132 GLHYDRLASE2
SUPFAMiSSF49303 SSF49303, 1 hit
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00719 GLYCOSYL_HYDROL_F2_1, 1 hit
PS00608 GLYCOSYL_HYDROL_F2_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBGLR_MOUSE
AccessioniPrimary (citable) accession number: P12265
Secondary accession number(s): Q61601
, Q64473, Q64474, Q6IR10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 3, 2012
Last modified: November 7, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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