UniProtKB - P12081 (HARS1_HUMAN)
Protein
Histidine--tRNA ligase, cytoplasmic
Gene
HARS1
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP) (PubMed:29235198). Plays a role in axon guidance (PubMed:26072516).2 Publications
Catalytic activityi
- EC:6.1.1.211 Publication
Kineticsi
kcat is 5.4 sec(-1) for aminoacylation of tRNA(His) (PubMed:29235198). kcat is 4.1 sec(-1) for histidine. (PubMed:29235198). kcat is 5.8 sec(-1) for ATP (PubMed:29235198).1 Publication
- KM=8.0 µM for histidine1 Publication
- KM=0.872 µM for tRNA(His)1 Publication
- KM=44.2 µM for ATP1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 157 | L-histidineCombined sources1 Publication | 1 | |
Binding sitei | 173 | L-histidineCombined sources1 Publication | 1 | |
Binding sitei | 177 | L-histidineCombined sources1 Publication | 1 | |
Binding sitei | 326 | L-histidineCombined sources1 Publication | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- histidine-tRNA ligase activity Source: UniProtKB
- identical protein binding Source: IntAct
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- histidyl-tRNA aminoacylation Source: UniProtKB
- mitochondrial translation Source: GO_Central
- translation Source: UniProtKB
- tRNA aminoacylation for protein translation Source: Reactome
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.1.1.21, 2681 |
PathwayCommonsi | P12081 |
Reactomei | R-HSA-379716, Cytosolic tRNA aminoacylation |
Names & Taxonomyi
Protein namesi | Recommended name: Histidine--tRNA ligase, cytoplasmic (EC:6.1.1.211 Publication)Alternative name(s): Histidyl-tRNA synthetase Short name: HisRS |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4816, HARS1 |
MIMi | 142810, gene |
neXtProti | NX_P12081 |
VEuPathDBi | HostDB:ENSG00000170445.12 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
Cytosol
- cytosol Source: HPA
Mitochondrion
- mitochondrion Source: GO_Central
Other locations
- cytoplasm Source: WormBase
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Involvement in diseasei
Usher syndrome 3B (USH3B)1 Publication
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionA syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life.
Related information in OMIMFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_067918 | 454 | Y → S in USH3B. 1 PublicationCorresponds to variant dbSNP:rs387906639EnsemblClinVar. | 1 |
Charcot-Marie-Tooth disease 2W (CMT2W)3 Publications
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionAn autosomal dominant, axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2W patients manifest a peripheral neuropathy mainly affecting the lower limbs and resulting in gait difficulties and distal sensory impairment. Most patients also have upper limb involvement.
Related information in OMIMFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_075064 | 132 | T → I in CMT2W; loss-of-function variant. 1 PublicationCorresponds to variant dbSNP:rs143473232EnsemblClinVar. | 1 | |
Natural variantiVAR_075065 | 134 | P → H in CMT2W; loss-of-function variant. 1 PublicationCorresponds to variant dbSNP:rs863225122EnsemblClinVar. | 1 | |
Natural variantiVAR_069022 | 137 | R → Q in CMT2W; has a neurotoxic effect in an animal model; results in loss of function. 1 PublicationCorresponds to variant dbSNP:rs191391414EnsemblClinVar. | 1 | |
Natural variantiVAR_083003 | 155 | V → G in CMT2W; unknown pathological significance; fails to complement deletion of the yeast ortholog; decreases histidine-tRNA ligase activity; increases in the KM for ATP binding; does not disrupt dimerization. 1 PublicationCorresponds to variant dbSNP:rs1239341211EnsemblClinVar. | 1 | |
Natural variantiVAR_075066 | 175 | D → E in CMT2W; hypomorphic variant. 1 PublicationCorresponds to variant dbSNP:rs863225123EnsemblClinVar. | 1 | |
Natural variantiVAR_069024 | 238 | V → A in CMT2W; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs536175170Ensembl. | 1 | |
Natural variantiVAR_083004 | 330 | Y → C in CMT2W; fails to complement deletion of the yeast ortholog; decreases histidine-tRNA ligase activity; increases in the KM for ATP binding; does not disrupt dimerization. 1 PublicationCorresponds to variant dbSNP:rs1554106881EnsemblClinVar. | 1 | |
Natural variantiVAR_083005 | 356 | S → N in CMT2W; unknown pathological significance; fails to complement deletion of the yeast ortholog; decreases histidine-tRNA ligase activity; increases in the KM for ATP binding; does not disrupt dimerization. 1 PublicationCorresponds to variant dbSNP:rs144322728EnsemblClinVar. | 1 | |
Natural variantiVAR_075067 | 364 | D → Y in CMT2W; loss-of-function variant. 1 PublicationCorresponds to variant dbSNP:rs863225124EnsemblClinVar. | 1 | |
Natural variantiVAR_069026 | 505 | P → S in CMT2W; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs747156884Ensembl. | 1 |
Keywords - Diseasei
Charcot-Marie-Tooth disease, Deafness, Disease variant, Neurodegeneration, Neuropathy, Retinitis pigmentosa, Usher syndromeOrganism-specific databases
DisGeNETi | 3035 |
GeneReviewsi | HARS |
MalaCardsi | HARS1 |
MIMi | 614504, phenotype 616625, phenotype |
OpenTargetsi | ENSG00000170445 |
Orphaneti | 488333, Autosomal dominant Charcot-Marie-Tooth disease type 2W 231183, Usher syndrome type 3 |
PharmGKBi | PA29191 |
Miscellaneous databases
Pharosi | P12081, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4002 |
DrugBanki | DB00117, Histidine |
Genetic variation databases
BioMutai | HARS |
DMDMi | 135123 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000136332 | 2 – 509 | Histidine--tRNA ligase, cytoplasmicAdd BLAST | 508 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 66 | PhosphoserineBy similarity | 1 | |
Modified residuei | 356 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P12081 |
jPOSTi | P12081 |
MassIVEi | P12081 |
MaxQBi | P12081 |
PaxDbi | P12081 |
PeptideAtlasi | P12081 |
PRIDEi | P12081 |
ProteomicsDBi | 12704 [P12081-1] 14349 52824 [P12081-1] 5502 |
PTM databases
iPTMneti | P12081 |
MetOSitei | P12081 |
PhosphoSitePlusi | P12081 |
SwissPalmi | P12081 |
Expressioni
Tissue specificityi
Brain, heart, liver and kidney.
Gene expression databases
Bgeei | ENSG00000170445, Expressed in adenohypophysis and 233 other tissues |
ExpressionAtlasi | P12081, baseline and differential |
Genevisiblei | P12081, HS |
Organism-specific databases
HPAi | ENSG00000170445, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
3 PublicationsBinary interactionsi
P12081
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-1057566,EBI-1057566 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 109285, 57 interactors |
DIPi | DIP-37596N |
IntActi | P12081, 11 interactors |
MINTi | P12081 |
STRINGi | 9606.ENSP00000425634 |
Chemistry databases
BindingDBi | P12081 |
Miscellaneous databases
RNActi | P12081, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P12081 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P12081 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 59 | WHEP-TRSAdd BLAST | 57 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 130 – 132 | L-histidine bindingCombined sources1 Publication | 3 | |
Regioni | 330 – 331 | L-histidine bindingCombined sources1 Publication | 2 |
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | KOG1936, Eukaryota |
GeneTreei | ENSGT00390000005922 |
InParanoidi | P12081 |
OMAi | CDFDFIG |
OrthoDBi | 1065556at2759 |
PhylomeDBi | P12081 |
TreeFami | TF300652 |
Family and domain databases
CDDi | cd00773, HisRS-like_core, 1 hit cd00859, HisRS_anticodon, 1 hit |
DisProti | DP01668 |
Gene3Di | 3.40.50.800, 1 hit |
HAMAPi | MF_00127, His_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR006195, aa-tRNA-synth_II IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR015807, His-tRNA-ligase IPR041715, HisRS-like_core IPR004516, HisRS/HisZ IPR033656, HisRS_anticodon IPR009068, S15_NS1_RNA-bd IPR000738, WHEP-TRS_dom |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF13393, tRNA-synt_His, 1 hit PF00458, WHEP-TRS, 1 hit |
PIRSFi | PIRSF001549, His-tRNA_synth, 1 hit |
SMARTi | View protein in SMART SM00991, WHEP-TRS, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit |
TIGRFAMsi | TIGR00442, hisS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit PS00762, WHEP_TRS_1, 1 hit PS51185, WHEP_TRS_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 14 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P12081-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAERAALEEL VKLQGERVRG LKQQKASAEL IEEEVAKLLK LKAQLGPDES
60 70 80 90 100
KQKFVLKTPK GTRDYSPRQM AVREKVFDVI IRCFKRHGAE VIDTPVFELK
110 120 130 140 150
ETLMGKYGED SKLIYDLKDQ GGELLSLRYD LTVPFARYLA MNKLTNIKRY
160 170 180 190 200
HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG NFDPMIPDAE CLKIMCEILS
210 220 230 240 250
SLQIGDFLVK VNDRRILDGM FAICGVSDSK FRTICSSVDK LDKVSWEEVK
260 270 280 290 300
NEMVGEKGLA PEVADRIGDY VQQHGGVSLV EQLLQDPKLS QNKQALEGLG
310 320 330 340 350
DLKLLFEYLT LFGIDDKISF DLSLARGLDY YTGVIYEAVL LQTPAQAGEE
360 370 380 390 400
PLGVGSVAAG GRYDGLVGMF DPKGRKVPCV GLSIGVERIF SIVEQRLEAL
410 420 430 440 450
EEKIRTTETQ VLVASAQKKL LEERLKLVSE LWDAGIKAEL LYKKNPKLLN
460 470 480 490 500
QLQYCEEAGI PLVAIIGEQE LKDGVIKLRS VTSREEVDVR REDLVEEIKR
RTGQPLCIC
Computationally mapped potential isoform sequencesi
There are 14 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB4DDD8 | B4DDD8_HUMAN | Histidine--tRNA ligase, cytoplasmic | HARS1 | 435 | Annotation score: | ||
B4E1C5 | B4E1C5_HUMAN | Histidine--tRNA ligase, cytoplasmic | HARS1 | 395 | Annotation score: | ||
E7ETE2 | E7ETE2_HUMAN | Histidine--tRNA ligase, cytoplasmic | HARS1 | 512 | Annotation score: | ||
A0A6Q8PF47 | A0A6Q8PF47_HUMAN | Histidine--tRNA ligase, cytoplasmic | HARS1 | 511 | Annotation score: | ||
A0A6Q8PHA5 | A0A6Q8PHA5_HUMAN | Histidine--tRNA ligase, cytoplasmic | HARS1 | 473 | Annotation score: | ||
A0A6Q8PHE3 | A0A6Q8PHE3_HUMAN | Histidine--tRNA ligase, cytoplasmic | HARS1 | 399 | Annotation score: | ||
A0A6Q8PF07 | A0A6Q8PF07_HUMAN | Histidine--tRNA ligase | HARS1 | 440 | Annotation score: | ||
A0A6Q8PFF2 | A0A6Q8PFF2_HUMAN | Histidine--tRNA ligase, cytoplasmic | HARS1 | 470 | Annotation score: | ||
D6RF05 | D6RF05_HUMAN | Histidine--tRNA ligase, cytoplasmic | HARS1 | 60 | Annotation score: | ||
A0A2R8YCI2 | A0A2R8YCI2_HUMAN | Histidine--tRNA ligase, cytoplasmic | HARS1 | 84 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence cautioni
The sequence CAA28956 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | A → P in CAA28956 (PubMed:3554142).Curated | 1 | |
Sequence conflicti | 126 | S → P in BAG58213 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 165 | R → G in CAA28956 (PubMed:3554142).Curated | 1 | |
Sequence conflicti | 181 | N → Q in CAA28956 (PubMed:3554142).Curated | 1 | |
Sequence conflicti | 186 | I → N in CAA28956 (PubMed:3554142).Curated | 1 | |
Sequence conflicti | 191 | C → S in CAA28956 (PubMed:3554142).Curated | 1 | |
Sequence conflicti | 206 | D → N in CAA28956 (PubMed:3554142).Curated | 1 | |
Sequence conflicti | 223 | I → V in CAA28956 (PubMed:3554142).Curated | 1 | |
Sequence conflicti | 227 | S → P in CAA28956 (PubMed:3554142).Curated | 1 | |
Sequence conflicti | 284 | L → V in CAA28956 (PubMed:3554142).Curated | 1 | |
Sequence conflicti | 347 | A → E in CAA28956 (PubMed:3554142).Curated | 1 | |
Sequence conflicti | 373 – 374 | KG → QR in CAA28956 (PubMed:3554142).Curated | 2 | |
Sequence conflicti | 375 | R → L in BAG58213 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 493 | D → E in CAA28956 (PubMed:3554142).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_069021 | 5 | A → E1 PublicationCorresponds to variant dbSNP:rs78741041EnsemblClinVar. | 1 | |
Natural variantiVAR_075064 | 132 | T → I in CMT2W; loss-of-function variant. 1 PublicationCorresponds to variant dbSNP:rs143473232EnsemblClinVar. | 1 | |
Natural variantiVAR_075065 | 134 | P → H in CMT2W; loss-of-function variant. 1 PublicationCorresponds to variant dbSNP:rs863225122EnsemblClinVar. | 1 | |
Natural variantiVAR_069022 | 137 | R → Q in CMT2W; has a neurotoxic effect in an animal model; results in loss of function. 1 PublicationCorresponds to variant dbSNP:rs191391414EnsemblClinVar. | 1 | |
Natural variantiVAR_083003 | 155 | V → G in CMT2W; unknown pathological significance; fails to complement deletion of the yeast ortholog; decreases histidine-tRNA ligase activity; increases in the KM for ATP binding; does not disrupt dimerization. 1 PublicationCorresponds to variant dbSNP:rs1239341211EnsemblClinVar. | 1 | |
Natural variantiVAR_075066 | 175 | D → E in CMT2W; hypomorphic variant. 1 PublicationCorresponds to variant dbSNP:rs863225123EnsemblClinVar. | 1 | |
Natural variantiVAR_069023 | 205 | G → D1 PublicationCorresponds to variant dbSNP:rs147288996EnsemblClinVar. | 1 | |
Natural variantiVAR_069024 | 238 | V → A in CMT2W; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs536175170Ensembl. | 1 | |
Natural variantiVAR_083004 | 330 | Y → C in CMT2W; fails to complement deletion of the yeast ortholog; decreases histidine-tRNA ligase activity; increases in the KM for ATP binding; does not disrupt dimerization. 1 PublicationCorresponds to variant dbSNP:rs1554106881EnsemblClinVar. | 1 | |
Natural variantiVAR_083005 | 356 | S → N in CMT2W; unknown pathological significance; fails to complement deletion of the yeast ortholog; decreases histidine-tRNA ligase activity; increases in the KM for ATP binding; does not disrupt dimerization. 1 PublicationCorresponds to variant dbSNP:rs144322728EnsemblClinVar. | 1 | |
Natural variantiVAR_075067 | 364 | D → Y in CMT2W; loss-of-function variant. 1 PublicationCorresponds to variant dbSNP:rs863225124EnsemblClinVar. | 1 | |
Natural variantiVAR_069025 | 376 | K → R1 PublicationCorresponds to variant dbSNP:rs139447495EnsemblClinVar. | 1 | |
Natural variantiVAR_061908 | 399 | A → V. Corresponds to variant dbSNP:rs34732372EnsemblClinVar. | 1 | |
Natural variantiVAR_067918 | 454 | Y → S in USH3B. 1 PublicationCorresponds to variant dbSNP:rs387906639EnsemblClinVar. | 1 | |
Natural variantiVAR_069026 | 505 | P → S in CMT2W; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs747156884Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045118 | 60 – 99 | Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST | 40 | |
Alternative sequenceiVSP_046662 | 155 – 174 | Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST | 20 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11518 mRNA Translation: CAA77607.1 X05345 mRNA Translation: CAA28956.1 Frameshift. AK295219 mRNA Translation: BAG58213.1 AK302295 mRNA Translation: BAG63635.1 AK225776 mRNA No translation available. AC116353 Genomic DNA No translation available. BC011807 mRNA Translation: AAH11807.1 BC080514 mRNA Translation: AAH80514.1 M96646 Genomic DNA Translation: AAA58668.1 U18936 Genomic DNA Translation: AAA73973.1 |
CCDSi | CCDS4237.1 [P12081-1] CCDS58976.1 [P12081-2] CCDS58977.1 [P12081-3] CCDS58978.1 [P12081-4] |
PIRi | I37559, SYHUHT |
RefSeqi | NP_001244969.1, NM_001258040.2 [P12081-2] NP_001244970.1, NM_001258041.2 [P12081-4] NP_001244971.1, NM_001258042.2 [P12081-3] NP_002100.2, NM_002109.5 [P12081-1] |
Genome annotation databases
Ensembli | ENST00000307633; ENSP00000304668; ENSG00000170445 [P12081-3] ENST00000438307; ENSP00000411511; ENSG00000170445 [P12081-2] ENST00000457527; ENSP00000387893; ENSG00000170445 [P12081-4] ENST00000504156; ENSP00000425634; ENSG00000170445 [P12081-1] |
GeneIDi | 3035 |
KEGGi | hsa:3035 |
UCSCi | uc003lgv.6, human [P12081-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11518 mRNA Translation: CAA77607.1 X05345 mRNA Translation: CAA28956.1 Frameshift. AK295219 mRNA Translation: BAG58213.1 AK302295 mRNA Translation: BAG63635.1 AK225776 mRNA No translation available. AC116353 Genomic DNA No translation available. BC011807 mRNA Translation: AAH11807.1 BC080514 mRNA Translation: AAH80514.1 M96646 Genomic DNA Translation: AAA58668.1 U18936 Genomic DNA Translation: AAA73973.1 |
CCDSi | CCDS4237.1 [P12081-1] CCDS58976.1 [P12081-2] CCDS58977.1 [P12081-3] CCDS58978.1 [P12081-4] |
PIRi | I37559, SYHUHT |
RefSeqi | NP_001244969.1, NM_001258040.2 [P12081-2] NP_001244970.1, NM_001258041.2 [P12081-4] NP_001244971.1, NM_001258042.2 [P12081-3] NP_002100.2, NM_002109.5 [P12081-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1X59 | NMR | - | A | 1-60 | [»] | |
2LW7 | NMR | - | A | 2-60 | [»] | |
A | 399-509 | [»] | ||||
4G84 | X-ray | 2.40 | A/B | 54-506 | [»] | |
4G85 | X-ray | 3.11 | A/B | 1-506 | [»] | |
4PHC | X-ray | 2.84 | A/B/C/D | 1-509 | [»] | |
4X5O | X-ray | 2.80 | A/B | 1-509 | [»] | |
5W6M | X-ray | 3.70 | A/B | 54-503 | [»] | |
6O76 | X-ray | 2.79 | A/B | 1-509 | [»] | |
SMRi | P12081 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109285, 57 interactors |
DIPi | DIP-37596N |
IntActi | P12081, 11 interactors |
MINTi | P12081 |
STRINGi | 9606.ENSP00000425634 |
Chemistry databases
BindingDBi | P12081 |
ChEMBLi | CHEMBL4002 |
DrugBanki | DB00117, Histidine |
PTM databases
iPTMneti | P12081 |
MetOSitei | P12081 |
PhosphoSitePlusi | P12081 |
SwissPalmi | P12081 |
Genetic variation databases
BioMutai | HARS |
DMDMi | 135123 |
Proteomic databases
EPDi | P12081 |
jPOSTi | P12081 |
MassIVEi | P12081 |
MaxQBi | P12081 |
PaxDbi | P12081 |
PeptideAtlasi | P12081 |
PRIDEi | P12081 |
ProteomicsDBi | 12704 [P12081-1] 14349 52824 [P12081-1] 5502 |
Protocols and materials databases
ABCDi | P12081, 2 sequenced antibodies |
Antibodypediai | 15372, 281 antibodies |
Genome annotation databases
Ensembli | ENST00000307633; ENSP00000304668; ENSG00000170445 [P12081-3] ENST00000438307; ENSP00000411511; ENSG00000170445 [P12081-2] ENST00000457527; ENSP00000387893; ENSG00000170445 [P12081-4] ENST00000504156; ENSP00000425634; ENSG00000170445 [P12081-1] |
GeneIDi | 3035 |
KEGGi | hsa:3035 |
UCSCi | uc003lgv.6, human [P12081-1] |
Organism-specific databases
CTDi | 3035 |
DisGeNETi | 3035 |
GeneCardsi | HARS1 |
GeneReviewsi | HARS |
HGNCi | HGNC:4816, HARS1 |
HPAi | ENSG00000170445, Low tissue specificity |
MalaCardsi | HARS1 |
MIMi | 142810, gene 614504, phenotype 616625, phenotype |
neXtProti | NX_P12081 |
OpenTargetsi | ENSG00000170445 |
Orphaneti | 488333, Autosomal dominant Charcot-Marie-Tooth disease type 2W 231183, Usher syndrome type 3 |
PharmGKBi | PA29191 |
VEuPathDBi | HostDB:ENSG00000170445.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1936, Eukaryota |
GeneTreei | ENSGT00390000005922 |
InParanoidi | P12081 |
OMAi | CDFDFIG |
OrthoDBi | 1065556at2759 |
PhylomeDBi | P12081 |
TreeFami | TF300652 |
Enzyme and pathway databases
BRENDAi | 6.1.1.21, 2681 |
PathwayCommonsi | P12081 |
Reactomei | R-HSA-379716, Cytosolic tRNA aminoacylation |
Miscellaneous databases
BioGRID-ORCSi | 3035, 697 hits in 877 CRISPR screens |
ChiTaRSi | HARS, human |
EvolutionaryTracei | P12081 |
GeneWikii | HARS |
GenomeRNAii | 3035 |
Pharosi | P12081, Tchem |
PROi | PR:P12081 |
RNActi | P12081, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000170445, Expressed in adenohypophysis and 233 other tissues |
ExpressionAtlasi | P12081, baseline and differential |
Genevisiblei | P12081, HS |
Family and domain databases
CDDi | cd00773, HisRS-like_core, 1 hit cd00859, HisRS_anticodon, 1 hit |
DisProti | DP01668 |
Gene3Di | 3.40.50.800, 1 hit |
HAMAPi | MF_00127, His_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR006195, aa-tRNA-synth_II IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR015807, His-tRNA-ligase IPR041715, HisRS-like_core IPR004516, HisRS/HisZ IPR033656, HisRS_anticodon IPR009068, S15_NS1_RNA-bd IPR000738, WHEP-TRS_dom |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF13393, tRNA-synt_His, 1 hit PF00458, WHEP-TRS, 1 hit |
PIRSFi | PIRSF001549, His-tRNA_synth, 1 hit |
SMARTi | View protein in SMART SM00991, WHEP-TRS, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit |
TIGRFAMsi | TIGR00442, hisS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit PS00762, WHEP_TRS_1, 1 hit PS51185, WHEP_TRS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HARS1_HUMAN | |
Accessioni | P12081Primary (citable) accession number: P12081 Secondary accession number(s): B4DHQ1 J3KNE5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | May 1, 1992 | |
Last modified: | February 10, 2021 | |
This is version 216 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families