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Protein

Tyrosine--tRNA ligase, mitochondrial

Gene

cyt-18

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). Has both an aminoacyl-tRNA synthetase activity and is involved in the splicing of group I introns. It acts in intron splicing by stabilizing the catalytically active structure of the intron.1 Publication

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99TyrosineBy similarity1
Binding sitei103ATPBy similarity1
Binding sitei143TyrosineBy similarity1
Binding sitei247TyrosineBy similarity1
Binding sitei251TyrosineBy similarity1
Binding sitei254TyrosineBy similarity1
Binding sitei273TyrosineBy similarity1
Binding sitei327ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • RNA binding Source: CAFA
  • tyrosine-tRNA ligase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processmRNA processing, Protein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.1 3627

Protein family/group databases

MoonDBiP12063 Curated
MoonProtiP12063

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligase, mitochondrial (EC:6.1.1.1)
Alternative name(s):
Tyrosyl-tRNA synthetase
Short name:
TyrRS
Gene namesi
Name:cyt-18
ORF Names:B18P24.070, NCU03030
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 1, Linkage Group I

Organism-specific databases

EuPathDBiFungiDB:NCU03030

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi127G → E: Splicing and aminoacylation defects (mutants C-18-1 and C-18-2). 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32MitochondrionAdd BLAST32
ChainiPRO_000003583333 – 669Tyrosine--tRNA ligase, mitochondrialAdd BLAST637

Proteomic databases

PRIDEiP12063

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-15678762,EBI-15678762

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-59835N

Structurei

Secondary structure

1669
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP12063
SMRiP12063
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12063

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 75Involved in splicingCuratedAdd BLAST43

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi104 – 113"HIGH" region10
Motifi324 – 328"KMSKS" region5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000242790
InParanoidiP12063
KOiK01866
OMAiMHMQLKK
OrthoDBiEOG092C2LDP

Family and domain databases

CDDicd00805 TyrRS_core, 1 hit
Gene3Di3.10.290.10, 1 hit
3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR036986 S4_RNA-bd_sf
IPR002307 Tyr-tRNA-ligase
IPR024088 Tyr-tRNA-ligase_bac-type
IPR032005 TyrRSs_C
PANTHERiPTHR11766 PTHR11766, 1 hit
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PF16714 TyrRSs_C, 1 hit
PRINTSiPR01040 TRNASYNTHTYR
TIGRFAMsiTIGR00234 tyrS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12063-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLLRTKALIR SGGSIAKYAA ANPSCFILQR RGLRREFGPK YTAKINEAEE
60 70 80 90 100
NWQARAEAIK KGKKQNTWDL FEERGYVKDT AGTKEHIAEL MRTRRIGAYV
110 120 130 140 150
GIDPTAPSLH VGHLLPLMPL FWMYLEGYKA FTLIGGSTAK IGDPTGRLKS
160 170 180 190 200
RDHLSSSDAT MNMTKIHYQL KKLWENVDTQ MRARGYEADW ARKRGIVNNN
210 220 230 240 250
HWWNKQPMLE VLRRVGHALR IGPMLSRDTV KNKMTQGDGV SFAEFTYPIM
260 270 280 290 300
QGWDWFELFY QQGVQMQIGG SDQYGNIISG LEVVKAARES EPDPQERKYV
310 320 330 340 350
TPKTALDECV GFTVPLLTDS SGAKFGKSAG NAIWLDPYQT SVFDFYGYFV
360 370 380 390 400
RRSDQEVENL LKLFTFMPIS EITKTMEEHI KDPSKRVAQH TLAREVVTLV
410 420 430 440 450
HGKQEASAAE DQHRMMYTGQ MTIPQVSRAK DAATGGDQYK TISDQPVTLN
460 470 480 490 500
NAPRIDMILP ESLIMGKSIG RILYAAGLAS STTEGHKLAA AQGCYVGGAH
510 520 530 540 550
RAGGENVTMN PDLISFMPVK LWFPGETQRY LINGNLLILR KGKHNVRVIQ
560 570 580 590 600
MVSDVEYAAS GQTYPGQSFT GAVRKLNEIM KNLKEKKLTP EEAKNAVNEL
610 620 630 640 650
QKSSQEKQQG QQIIFPEEKS RQKKDMETKL KQEMIASVKT IDGMMDEKPS
660
VRGDGVKKQT QDDRDPYKW
Length:669
Mass (Da):75,422
Last modified:February 1, 1996 - v2
Checksum:iDD136CAC9AD1BEB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17118 Genomic DNA Translation: AAA33620.1
BX842626 Genomic DNA Translation: CAE76265.1
CM002236 Genomic DNA Translation: EAA36180.1
PIRiA27158 SYNCYT
RefSeqiXP_965416.1, XM_960323.2

Genome annotation databases

EnsemblFungiiEAA36180; EAA36180; NCU03030
GeneIDi3881566
KEGGincr:NCU03030

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17118 Genomic DNA Translation: AAA33620.1
BX842626 Genomic DNA Translation: CAE76265.1
CM002236 Genomic DNA Translation: EAA36180.1
PIRiA27158 SYNCYT
RefSeqiXP_965416.1, XM_960323.2

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y42X-ray1.95X33-423[»]
2RKJX-ray4.50A/B/E/F/I/J/M/N33-423[»]
4OJMX-ray1.95X33-423[»]
ProteinModelPortaliP12063
SMRiP12063
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59835N

Protein family/group databases

MoonDBiP12063 Curated
MoonProtiP12063

Proteomic databases

PRIDEiP12063

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAA36180; EAA36180; NCU03030
GeneIDi3881566
KEGGincr:NCU03030

Organism-specific databases

EuPathDBiFungiDB:NCU03030

Phylogenomic databases

HOGENOMiHOG000242790
InParanoidiP12063
KOiK01866
OMAiMHMQLKK
OrthoDBiEOG092C2LDP

Enzyme and pathway databases

BRENDAi6.1.1.1 3627

Miscellaneous databases

EvolutionaryTraceiP12063

Family and domain databases

CDDicd00805 TyrRS_core, 1 hit
Gene3Di3.10.290.10, 1 hit
3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR036986 S4_RNA-bd_sf
IPR002307 Tyr-tRNA-ligase
IPR024088 Tyr-tRNA-ligase_bac-type
IPR032005 TyrRSs_C
PANTHERiPTHR11766 PTHR11766, 1 hit
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PF16714 TyrRSs_C, 1 hit
PRINTSiPR01040 TRNASYNTHTYR
TIGRFAMsiTIGR00234 tyrS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSYYM_NEUCR
AccessioniPrimary (citable) accession number: P12063
Secondary accession number(s): Q7RVM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: February 1, 1996
Last modified: July 18, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
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