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UniProtKB - P12035 (K2C3_HUMAN)
Protein
Keratin, type II cytoskeletal 3
Gene
KRT3
Organism
Homo sapiens (Human)
Status
Functioni
Miscellaneous
There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
GO - Biological processi
- epithelial cell differentiation Source: UniProtKB
- intermediate filament cytoskeleton organization Source: UniProtKB
Enzyme and pathway databases
PathwayCommonsi | P12035 |
Reactomei | R-HSA-6805567, Keratinization R-HSA-6809371, Formation of the cornified envelope |
SignaLinki | P12035 |
Names & Taxonomyi
Protein namesi | Recommended name: Keratin, type II cytoskeletal 3Alternative name(s): 65 kDa cytokeratin Cytokeratin-3 Short name: CK-3 Keratin-3 Short name: K3 Type-II keratin Kb3 |
Gene namesi | Name:KRT3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6440, KRT3 |
MIMi | 148043, gene |
neXtProti | NX_P12035 |
VEuPathDBi | HostDB:ENSG00000186442 |
Subcellular locationi
Cytoskeleton
- intermediate filament Source: UniProtKB
- keratin filament Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Keywords - Cellular componenti
Intermediate filament, KeratinPathology & Biotechi
Involvement in diseasei
Corneal dystrophy, Meesmann 2 (MECD2)2 Publications
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionA form of Meesmann corneal dystrophy, a corneal disease characterized by fragility of the anterior corneal epithelium. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. MECD2 inheritance is autosomal dominant.
Related information in OMIMFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_031327 | 503 | R → P in MECD2. 1 PublicationCorresponds to variant dbSNP:rs60410063EnsemblClinVar. | 1 | |
Natural variantiVAR_003868 | 509 | E → K in MECD2. 1 PublicationCorresponds to variant dbSNP:rs57872071EnsemblClinVar. | 1 |
Keywords - Diseasei
Corneal dystrophy, Disease variantOrganism-specific databases
DisGeNETi | 3850 |
MalaCardsi | KRT3 |
MIMi | 618767, phenotype |
OpenTargetsi | ENSG00000186442 |
Orphaneti | 98954, Meesmann corneal dystrophy |
PharmGKBi | PA30228 |
Miscellaneous databases
Pharosi | P12035, Tbio |
Genetic variation databases
BioMutai | KRT3 |
DMDMi | 313104225 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063716 | 1 – 628 | Keratin, type II cytoskeletal 3Add BLAST | 628 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 13 | PhosphoserineBy similarity | 1 | |
Modified residuei | 56 | PhosphoserineBy similarity | 1 | |
Modified residuei | 296 | N6,N6-dimethyllysineBy similarity | 1 | |
Modified residuei | 364 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
jPOSTi | P12035 |
MassIVEi | P12035 |
PaxDbi | P12035 |
PeptideAtlasi | P12035 |
PRIDEi | P12035 |
ProteomicsDBi | 52820 |
PTM databases
GlyGeni | P12035, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P12035 |
PhosphoSitePlusi | P12035 |
SwissPalmi | P12035 |
Expressioni
Tissue specificityi
Cornea specific.
Gene expression databases
Bgeei | ENSG00000186442, Expressed in gingiva and 37 other tissues |
Genevisiblei | P12035, HS |
Organism-specific databases
HPAi | ENSG00000186442, Group enriched (esophagus, salivary gland) |
Interactioni
Subunit structurei
Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.
Binary interactionsi
P12035
Protein-protein interaction databases
BioGRIDi | 110048, 85 interactors |
IntActi | P12035, 46 interactors |
STRINGi | 9606.ENSP00000413479 |
Miscellaneous databases
RNActi | P12035, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 198 – 513 | IF rodPROSITE-ProRule annotationAdd BLAST | 316 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 197 | HeadAdd BLAST | 197 | |
Regioni | 1 – 21 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 198 – 233 | Coil 1AAdd BLAST | 36 | |
Regioni | 234 – 254 | Linker 1Add BLAST | 21 | |
Regioni | 255 – 346 | Coil 1BAdd BLAST | 92 | |
Regioni | 347 – 370 | Linker 12Add BLAST | 24 | |
Regioni | 371 – 509 | Coil 2Add BLAST | 139 | |
Regioni | 510 – 628 | TailAdd BLAST | 119 | |
Regioni | 605 – 628 | DisorderedSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Belongs to the intermediate filament family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QURK, Eukaryota |
GeneTreei | ENSGT00940000162629 |
HOGENOMi | CLU_012560_6_0_1 |
InParanoidi | P12035 |
OMAi | DSEMRNM |
OrthoDBi | 824246at2759 |
PhylomeDBi | P12035 |
TreeFami | TF332742 |
Family and domain databases
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR032444, Keratin_2_head IPR003054, Keratin_II |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF16208, Keratin_2_head, 2 hits |
PRINTSi | PR01276, TYPE2KERATIN |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P12035-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRQASKTSG GGSQGFSGRS AVVSGSSRMS CVAHSGGAGG GAYGFRSGAG
60 70 80 90 100
GFGSRSLYNL GGNKSISISV AAGGSRAGGF GGGRSSCAFA GGYGGGFGSG
110 120 130 140 150
YGGGFGGGFG GGRGMGGGFG GAGGFGGAGG FGGAGGFGGP GGFGGSGGFG
160 170 180 190 200
GPGSLGSPGG FGPGGFPGGI QEVTINQSLL QPLNVEIDPQ IGQVKAQERE
210 220 230 240 250
QIKTLNNKFA SFIDKVRFLE QQNKVLETKW NLLQQQGTSS ISGTNNLEPL
260 270 280 290 300
FENHINYLRS YLDNILGERG RLDSELKNME DLVEDFKKKY EDEINKRTAA
310 320 330 340 350
ENEFVTLKKD VDSAYMNKVE LQAKVDALID EIDFLRTLYD AELSQMQSHI
360 370 380 390 400
SDTSVVLSMD NNRSLDLDSI IAEVRAQYED IAQRSKAEAE ALYQTKLGEL
410 420 430 440 450
QTTAGRHGDD LRNTKSEIIE LNRMIQRLRA EIEGVKKQNA NLQTAIAEAE
460 470 480 490 500
QHGEMALKDA NAKLQELQAA LQQAKDDLAR LLRDYQELMN VKLALDVEIA
510 520 530 540 550
TYRKLLEGEE YRMSGECPSA VSISVVSSST TSASAGGYGG GYGGGMGGGL
560 570 580 590 600
GGGFSAGGGS GSGFGRGGGG GIGGGFGGGS SGFSGGSGFG SISGARYGVS
610 620
GGGFSSASNR GGSIKFSQSS QSSQRYSR
Sequence cautioni
The sequence CAF31522 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 162 | G → A in CAA28991 (PubMed:2439698).Curated | 1 | |
Sequence conflicti | 175 | I → T in CAA28991 (PubMed:2439698).Curated | 1 | |
Sequence conflicti | 184 | N → K in CAA28991 (PubMed:2439698).Curated | 1 | |
Sequence conflicti | 187 | I → T in CAA28991 (PubMed:2439698).Curated | 1 | |
Sequence conflicti | 298 | T → Y in CAA28993 (PubMed:2439698).Curated | 1 | |
Sequence conflicti | 397 | L → M in CAA28996 (PubMed:2439698).Curated | 1 | |
Sequence conflicti | 409 | D → G in CAF31522 (PubMed:15737194).Curated | 1 | |
Sequence conflicti | 448 | E → Q in CAA28996 (PubMed:2439698).Curated | 1 | |
Sequence conflicti | 511 | Y → YS in CAA28996 (PubMed:2439698).Curated | 1 | |
Sequence conflicti | 562 | S → I in CAA28996 (PubMed:2439698).Curated | 1 | |
Sequence conflicti | 580 | S → T in CAA28996 (PubMed:2439698).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_061297 | 44 | G → A. Corresponds to variant dbSNP:rs28721426Ensembl. | 1 | |
Natural variantiVAR_056023 | 375 | R → G1 PublicationCorresponds to variant dbSNP:rs3887954Ensembl. | 1 | |
Natural variantiVAR_031327 | 503 | R → P in MECD2. 1 PublicationCorresponds to variant dbSNP:rs60410063EnsemblClinVar. | 1 | |
Natural variantiVAR_003868 | 509 | E → K in MECD2. 1 PublicationCorresponds to variant dbSNP:rs57872071EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05418 Genomic DNA Translation: CAA28991.1 X05419 Genomic DNA Translation: CAA28992.1 X05420 Genomic DNA Translation: CAA28993.1 X05420 Genomic DNA Translation: CAA28994.1 X05420 Genomic DNA Translation: CAA28995.1 X05421 Genomic DNA Translation: CAA28996.1 AC107016 Genomic DNA No translation available. AJ628418 mRNA Translation: CAF31522.1 Different initiation. |
CCDSi | CCDS44895.1 |
PIRi | A29666 |
RefSeqi | NP_476429.2, NM_057088.2 |
Genome annotation databases
Ensembli | ENST00000417996.2; ENSP00000413479.2; ENSG00000186442.7 |
GeneIDi | 3850 |
KEGGi | hsa:3850 |
MANE-Selecti | ENST00000417996.2; ENSP00000413479.2; NM_057088.3; NP_476429.2 |
UCSCi | uc001say.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Human Intermediate Filament Mutation Database |
Wikipedia Keratin-3 entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05418 Genomic DNA Translation: CAA28991.1 X05419 Genomic DNA Translation: CAA28992.1 X05420 Genomic DNA Translation: CAA28993.1 X05420 Genomic DNA Translation: CAA28994.1 X05420 Genomic DNA Translation: CAA28995.1 X05421 Genomic DNA Translation: CAA28996.1 AC107016 Genomic DNA No translation available. AJ628418 mRNA Translation: CAF31522.1 Different initiation. |
CCDSi | CCDS44895.1 |
PIRi | A29666 |
RefSeqi | NP_476429.2, NM_057088.2 |
3D structure databases
AlphaFoldDBi | P12035 |
SMRi | P12035 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 110048, 85 interactors |
IntActi | P12035, 46 interactors |
STRINGi | 9606.ENSP00000413479 |
PTM databases
GlyGeni | P12035, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P12035 |
PhosphoSitePlusi | P12035 |
SwissPalmi | P12035 |
Genetic variation databases
BioMutai | KRT3 |
DMDMi | 313104225 |
Proteomic databases
jPOSTi | P12035 |
MassIVEi | P12035 |
PaxDbi | P12035 |
PeptideAtlasi | P12035 |
PRIDEi | P12035 |
ProteomicsDBi | 52820 |
Protocols and materials databases
Antibodypediai | 7889, 178 antibodies from 27 providers |
DNASUi | 3850 |
Genome annotation databases
Ensembli | ENST00000417996.2; ENSP00000413479.2; ENSG00000186442.7 |
GeneIDi | 3850 |
KEGGi | hsa:3850 |
MANE-Selecti | ENST00000417996.2; ENSP00000413479.2; NM_057088.3; NP_476429.2 |
UCSCi | uc001say.4, human |
Organism-specific databases
CTDi | 3850 |
DisGeNETi | 3850 |
GeneCardsi | KRT3 |
HGNCi | HGNC:6440, KRT3 |
HPAi | ENSG00000186442, Group enriched (esophagus, salivary gland) |
MalaCardsi | KRT3 |
MIMi | 148043, gene 618767, phenotype |
neXtProti | NX_P12035 |
OpenTargetsi | ENSG00000186442 |
Orphaneti | 98954, Meesmann corneal dystrophy |
PharmGKBi | PA30228 |
VEuPathDBi | HostDB:ENSG00000186442 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QURK, Eukaryota |
GeneTreei | ENSGT00940000162629 |
HOGENOMi | CLU_012560_6_0_1 |
InParanoidi | P12035 |
OMAi | DSEMRNM |
OrthoDBi | 824246at2759 |
PhylomeDBi | P12035 |
TreeFami | TF332742 |
Enzyme and pathway databases
PathwayCommonsi | P12035 |
Reactomei | R-HSA-6805567, Keratinization R-HSA-6809371, Formation of the cornified envelope |
SignaLinki | P12035 |
Miscellaneous databases
BioGRID-ORCSi | 3850, 10 hits in 1065 CRISPR screens |
GeneWikii | Keratin_3 |
GenomeRNAii | 3850 |
Pharosi | P12035, Tbio |
PROi | PR:P12035 |
RNActi | P12035, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000186442, Expressed in gingiva and 37 other tissues |
Genevisiblei | P12035, HS |
Family and domain databases
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR032444, Keratin_2_head IPR003054, Keratin_II |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF16208, Keratin_2_head, 2 hits |
PRINTSi | PR01276, TYPE2KERATIN |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | K2C3_HUMAN | |
Accessioni | P12035Primary (citable) accession number: P12035 Secondary accession number(s): A6NIS2, Q701L8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | November 30, 2010 | |
Last modified: | May 25, 2022 | |
This is version 198 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families