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Protein

Keratin, type II cytoskeletal 3

Gene

KRT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

GO - Molecular functioni

  • structural molecule activity Source: UniProtKB

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-6805567 Keratinization
R-HSA-6809371 Formation of the cornified envelope

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type II cytoskeletal 3
Alternative name(s):
65 kDa cytokeratin
Cytokeratin-3
Short name:
CK-3
Keratin-3
Short name:
K3
Type-II keratin Kb3
Gene namesi
Name:KRT3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000186442.7
HGNCiHGNC:6440 KRT3
MIMi148043 gene
neXtProtiNX_P12035

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Intermediate filament, Keratin

Pathology & Biotechi

Involvement in diseasei

Corneal dystrophy, Meesmann (MECD)2 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autosomal dominant corneal disease characterized by fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.
See also OMIM:122100
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031327503R → P in MECD. 1 PublicationCorresponds to variant dbSNP:rs60410063EnsemblClinVar.1
Natural variantiVAR_003868509E → K in MECD. 1 PublicationCorresponds to variant dbSNP:rs57872071EnsemblClinVar.1

Keywords - Diseasei

Corneal dystrophy, Disease mutation

Organism-specific databases

DisGeNETi3850
MalaCardsiKRT3
MIMi122100 phenotype
OpenTargetsiENSG00000186442
Orphaneti98954 Meesmann corneal dystrophy
PharmGKBiPA30228

Polymorphism and mutation databases

BioMutaiKRT3
DMDMi313104225

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000637161 – 628Keratin, type II cytoskeletal 3Add BLAST628

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphoserineBy similarity1
Modified residuei56PhosphoserineBy similarity1
Modified residuei296N6,N6-dimethyllysineBy similarity1
Modified residuei364PhosphoserineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiP12035
PeptideAtlasiP12035
PRIDEiP12035
ProteomicsDBi52820

PTM databases

iPTMnetiP12035
PhosphoSitePlusiP12035
SwissPalmiP12035

Expressioni

Tissue specificityi

Cornea specific.

Gene expression databases

BgeeiENSG00000186442 Expressed in 27 organ(s), highest expression level in gingiva
CleanExiHS_KRT3
GenevisibleiP12035 HS

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.

Protein-protein interaction databases

BioGridi110048, 18 interactors
IntActiP12035, 45 interactors
STRINGi9606.ENSP00000413479

Structurei

3D structure databases

ProteinModelPortaliP12035
SMRiP12035
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini198 – 513IF rodPROSITE-ProRule annotationAdd BLAST316

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 197HeadAdd BLAST197
Regioni198 – 233Coil 1AAdd BLAST36
Regioni234 – 254Linker 1Add BLAST21
Regioni255 – 346Coil 1BAdd BLAST92
Regioni347 – 370Linker 12Add BLAST24
Regioni371 – 509Coil 2Add BLAST139
Regioni510 – 628TailAdd BLAST119

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi10 – 169Gly-richAdd BLAST160
Compositional biasi536 – 612Gly-richAdd BLAST77

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IKBD Eukaryota
ENOG4111UGN LUCA
GeneTreeiENSGT00760000118796
HOGENOMiHOG000230976
HOVERGENiHBG013015
InParanoidiP12035
KOiK07605
OMAiLFENHIN
OrthoDBiEOG091G09KR
PhylomeDBiP12035
TreeFamiTF332742

Family and domain databases

InterProiView protein in InterPro
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR032444 Keratin_2_head
IPR003054 Keratin_II
PANTHERiPTHR23239 PTHR23239, 2 hits
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF16208 Keratin_2_head, 2 hits
PRINTSiPR01276 TYPE2KERATIN
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit

Sequencei

Sequence statusi: Complete.

P12035-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRQASKTSG GGSQGFSGRS AVVSGSSRMS CVAHSGGAGG GAYGFRSGAG
60 70 80 90 100
GFGSRSLYNL GGNKSISISV AAGGSRAGGF GGGRSSCAFA GGYGGGFGSG
110 120 130 140 150
YGGGFGGGFG GGRGMGGGFG GAGGFGGAGG FGGAGGFGGP GGFGGSGGFG
160 170 180 190 200
GPGSLGSPGG FGPGGFPGGI QEVTINQSLL QPLNVEIDPQ IGQVKAQERE
210 220 230 240 250
QIKTLNNKFA SFIDKVRFLE QQNKVLETKW NLLQQQGTSS ISGTNNLEPL
260 270 280 290 300
FENHINYLRS YLDNILGERG RLDSELKNME DLVEDFKKKY EDEINKRTAA
310 320 330 340 350
ENEFVTLKKD VDSAYMNKVE LQAKVDALID EIDFLRTLYD AELSQMQSHI
360 370 380 390 400
SDTSVVLSMD NNRSLDLDSI IAEVRAQYED IAQRSKAEAE ALYQTKLGEL
410 420 430 440 450
QTTAGRHGDD LRNTKSEIIE LNRMIQRLRA EIEGVKKQNA NLQTAIAEAE
460 470 480 490 500
QHGEMALKDA NAKLQELQAA LQQAKDDLAR LLRDYQELMN VKLALDVEIA
510 520 530 540 550
TYRKLLEGEE YRMSGECPSA VSISVVSSST TSASAGGYGG GYGGGMGGGL
560 570 580 590 600
GGGFSAGGGS GSGFGRGGGG GIGGGFGGGS SGFSGGSGFG SISGARYGVS
610 620
GGGFSSASNR GGSIKFSQSS QSSQRYSR
Length:628
Mass (Da):64,417
Last modified:November 30, 2010 - v3
Checksum:i65FDB8CAA7F3C960
GO

Sequence cautioni

The sequence CAF31522 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162G → A in CAA28991 (PubMed:2439698).Curated1
Sequence conflicti175I → T in CAA28991 (PubMed:2439698).Curated1
Sequence conflicti184N → K in CAA28991 (PubMed:2439698).Curated1
Sequence conflicti187I → T in CAA28991 (PubMed:2439698).Curated1
Sequence conflicti298T → Y in CAA28993 (PubMed:2439698).Curated1
Sequence conflicti397L → M in CAA28996 (PubMed:2439698).Curated1
Sequence conflicti409D → G in CAF31522 (PubMed:15737194).Curated1
Sequence conflicti448E → Q in CAA28996 (PubMed:2439698).Curated1
Sequence conflicti511Y → YS in CAA28996 (PubMed:2439698).Curated1
Sequence conflicti562S → I in CAA28996 (PubMed:2439698).Curated1
Sequence conflicti580S → T in CAA28996 (PubMed:2439698).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06129744G → A. Corresponds to variant dbSNP:rs28721426Ensembl.1
Natural variantiVAR_056023375R → G1 PublicationCorresponds to variant dbSNP:rs3887954Ensembl.1
Natural variantiVAR_031327503R → P in MECD. 1 PublicationCorresponds to variant dbSNP:rs60410063EnsemblClinVar.1
Natural variantiVAR_003868509E → K in MECD. 1 PublicationCorresponds to variant dbSNP:rs57872071EnsemblClinVar.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05418 Genomic DNA Translation: CAA28991.1
X05419 Genomic DNA Translation: CAA28992.1
X05420 Genomic DNA Translation: CAA28993.1
X05420 Genomic DNA Translation: CAA28994.1
X05420 Genomic DNA Translation: CAA28995.1
X05421 Genomic DNA Translation: CAA28996.1
AC107016 Genomic DNA No translation available.
AJ628418 mRNA Translation: CAF31522.1 Different initiation.
CCDSiCCDS44895.1
PIRiA29666
RefSeqiNP_476429.2, NM_057088.2
UniGeneiHs.680652

Genome annotation databases

EnsembliENST00000417996; ENSP00000413479; ENSG00000186442
GeneIDi3850
KEGGihsa:3850
UCSCiuc001say.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Human Intermediate Filament Mutation Database
Wikipedia

Keratin-3 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05418 Genomic DNA Translation: CAA28991.1
X05419 Genomic DNA Translation: CAA28992.1
X05420 Genomic DNA Translation: CAA28993.1
X05420 Genomic DNA Translation: CAA28994.1
X05420 Genomic DNA Translation: CAA28995.1
X05421 Genomic DNA Translation: CAA28996.1
AC107016 Genomic DNA No translation available.
AJ628418 mRNA Translation: CAF31522.1 Different initiation.
CCDSiCCDS44895.1
PIRiA29666
RefSeqiNP_476429.2, NM_057088.2
UniGeneiHs.680652

3D structure databases

ProteinModelPortaliP12035
SMRiP12035
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110048, 18 interactors
IntActiP12035, 45 interactors
STRINGi9606.ENSP00000413479

PTM databases

iPTMnetiP12035
PhosphoSitePlusiP12035
SwissPalmiP12035

Polymorphism and mutation databases

BioMutaiKRT3
DMDMi313104225

Proteomic databases

PaxDbiP12035
PeptideAtlasiP12035
PRIDEiP12035
ProteomicsDBi52820

Protocols and materials databases

DNASUi3850
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000417996; ENSP00000413479; ENSG00000186442
GeneIDi3850
KEGGihsa:3850
UCSCiuc001say.4 human

Organism-specific databases

CTDi3850
DisGeNETi3850
EuPathDBiHostDB:ENSG00000186442.7
GeneCardsiKRT3
H-InvDBiHIX0036742
HGNCiHGNC:6440 KRT3
MalaCardsiKRT3
MIMi122100 phenotype
148043 gene
neXtProtiNX_P12035
OpenTargetsiENSG00000186442
Orphaneti98954 Meesmann corneal dystrophy
PharmGKBiPA30228
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IKBD Eukaryota
ENOG4111UGN LUCA
GeneTreeiENSGT00760000118796
HOGENOMiHOG000230976
HOVERGENiHBG013015
InParanoidiP12035
KOiK07605
OMAiLFENHIN
OrthoDBiEOG091G09KR
PhylomeDBiP12035
TreeFamiTF332742

Enzyme and pathway databases

ReactomeiR-HSA-6805567 Keratinization
R-HSA-6809371 Formation of the cornified envelope

Miscellaneous databases

GeneWikiiKeratin_3
GenomeRNAii3850
PROiPR:P12035
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000186442 Expressed in 27 organ(s), highest expression level in gingiva
CleanExiHS_KRT3
GenevisibleiP12035 HS

Family and domain databases

InterProiView protein in InterPro
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR032444 Keratin_2_head
IPR003054 Keratin_II
PANTHERiPTHR23239 PTHR23239, 2 hits
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF16208 Keratin_2_head, 2 hits
PRINTSiPR01276 TYPE2KERATIN
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiK2C3_HUMAN
AccessioniPrimary (citable) accession number: P12035
Secondary accession number(s): A6NIS2, Q701L8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: November 30, 2010
Last modified: September 12, 2018
This is version 175 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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Main funding by: National Institutes of Health

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