KRT3

Functionⁱ

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

GO - Molecular functionⁱ

structural molecule activity
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 9171831

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cornification Source: Reactome
epithelial cell differentiation Source: UniProtKB
intermediate filament cytoskeleton organization
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 9171831
keratinization Source: Reactome

View the complete GO annotation on QuickGO ...

Enzyme and pathway databases

Reactomeⁱ	R-HSA-6805567 Keratinization R-HSA-6809371 Formation of the cornified envelope

Reactomeⁱ

R-HSA-6805567 Keratinization
R-HSA-6809371 Formation of the cornified envelope

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Keratin, type II cytoskeletal 3 Alternative name(s): 65 kDa cytokeratin Cytokeratin-3 Short name: CK-3 Keratin-3 Short name: K3 Type-II keratin Kb3
Gene namesⁱ	Name:KRT3
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 12

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000186442.7
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:6440 KRT3
MIMⁱ	148043 gene
neXtProtⁱ	NX_P12035

Subcellular locationⁱ

GO - Cellular component

Keywords - Cellular componentⁱ

Intermediate filament, Keratin

Pathology & Biotechⁱ

Involvement in diseaseⁱ

Corneal dystrophy, Meesmann (MECD)2 Publications

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionAn autosomal dominant corneal disease characterized by fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_031327	503	R → P in MECD. 1 PublicationCorresponds to variant dbSNP:rs60410063EnsemblClinVar.		1
Natural variantⁱVAR_003868	509	E → K in MECD. 1 PublicationCorresponds to variant dbSNP:rs57872071EnsemblClinVar.		1

Keywords - Diseaseⁱ

Corneal dystrophy, Disease mutation

Organism-specific databases

DisGeNET More...DisGeNETⁱ	3850
MalaCards human disease database More...MalaCardsⁱ	KRT3
MIMⁱ	122100 phenotype
Open Targets More...OpenTargetsⁱ	ENSG00000186442
Orphanetⁱ	98954 Meesmann corneal dystrophy
PharmGKBⁱ	PA30228

Polymorphism and mutation databases

BioMutaⁱ	KRT3
DMDMⁱ	313104225

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000063716	1 – 628	Keratin, type II cytoskeletal 3Add BLAST		628

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	13	PhosphoserineBy similarity	1
Modified residueⁱ	56	PhosphoserineBy similarity	1
Modified residueⁱ	296	N6,N6-dimethyllysineBy similarity	1
Modified residueⁱ	364	PhosphoserineBy similarity	1

Keywords - PTMⁱ

Methylation, Phosphoprotein

Proteomic databases

PaxDbⁱ	P12035
PeptideAtlas More...PeptideAtlasⁱ	P12035
PRIDEⁱ	P12035
ProteomicsDBⁱ	52820

PTM databases

iPTMnetⁱ	P12035
PhosphoSitePlusⁱ	P12035
SwissPalmⁱ	P12035

Expressionⁱ

Tissue specificityⁱ

Cornea specific.

Gene expression databases

Bgeeⁱ	ENSG00000186442 Expressed in 27 organ(s), highest expression level in gingiva
CleanExⁱ	HS_KRT3
Genevisibleⁱ	P12035 HS

Interactionⁱ

Subunit structureⁱ

Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.

Protein-protein interaction databases

BioGridⁱ	110048, 18 interactors
IntActⁱ	P12035, 45 interactors
STRINGⁱ	9606.ENSP00000413479

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P12035
SMRⁱ	P12035
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	198 – 513	IF rodPROSITE-ProRule annotationAdd BLAST		316

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 197	HeadAdd BLAST	197
Regionⁱ	198 – 233	Coil 1AAdd BLAST	36
Regionⁱ	234 – 254	Linker 1Add BLAST	21
Regionⁱ	255 – 346	Coil 1BAdd BLAST	92
Regionⁱ	347 – 370	Linker 12Add BLAST	24
Regionⁱ	371 – 509	Coil 2Add BLAST	139
Regionⁱ	510 – 628	TailAdd BLAST	119

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	10 – 169	Gly-richAdd BLAST		160
Compositional biasⁱ	536 – 612	Gly-richAdd BLAST		77

Sequence similaritiesⁱ

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	ENOG410IKBD Eukaryota ENOG4111UGN LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118796
HOGENOMⁱ	HOG000230976
HOVERGENⁱ	HBG013015
InParanoidⁱ	P12035
KEGG Orthology (KO) More...KOⁱ	K07605
OMAⁱ	DSEMRNM
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G09KR
PhylomeDBⁱ	P12035
TreeFamⁱ	TF332742

Family and domain databases

InterProⁱ	View protein in InterPro IPR001664 IF IPR018039 IF_conserved IPR039008 IF_rod_dom IPR032444 Keratin_2_head IPR003054 Keratin_II
PANTHERⁱ	PTHR23239 PTHR23239, 2 hits
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00038 Filament, 1 hit PF16208 Keratin_2_head, 2 hits
PRINTSⁱ	PR01276 TYPE2KERATIN
SMARTⁱ	View protein in SMART SM01391 Filament, 1 hit
PROSITEⁱ	View protein in PROSITE PS00226 IF_ROD_1, 1 hit PS51842 IF_ROD_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P12035-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSRQASKTSG GGSQGFSGRS AVVSGSSRMS CVAHSGGAGG GAYGFRSGAG 
        60         70         80         90        100
GFGSRSLYNL GGNKSISISV AAGGSRAGGF GGGRSSCAFA GGYGGGFGSG 
       110        120        130        140        150
YGGGFGGGFG GGRGMGGGFG GAGGFGGAGG FGGAGGFGGP GGFGGSGGFG 
       160        170        180        190        200
GPGSLGSPGG FGPGGFPGGI QEVTINQSLL QPLNVEIDPQ IGQVKAQERE 
       210        220        230        240        250
QIKTLNNKFA SFIDKVRFLE QQNKVLETKW NLLQQQGTSS ISGTNNLEPL 
       260        270        280        290        300
FENHINYLRS YLDNILGERG RLDSELKNME DLVEDFKKKY EDEINKRTAA 
       310        320        330        340        350
ENEFVTLKKD VDSAYMNKVE LQAKVDALID EIDFLRTLYD AELSQMQSHI 
       360        370        380        390        400
SDTSVVLSMD NNRSLDLDSI IAEVRAQYED IAQRSKAEAE ALYQTKLGEL 
       410        420        430        440        450
QTTAGRHGDD LRNTKSEIIE LNRMIQRLRA EIEGVKKQNA NLQTAIAEAE 
       460        470        480        490        500
QHGEMALKDA NAKLQELQAA LQQAKDDLAR LLRDYQELMN VKLALDVEIA 
       510        520        530        540        550
TYRKLLEGEE YRMSGECPSA VSISVVSSST TSASAGGYGG GYGGGMGGGL 
       560        570        580        590        600
GGGFSAGGGS GSGFGRGGGG GIGGGFGGGS SGFSGGSGFG SISGARYGVS 
       610        620 
GGGFSSASNR GGSIKFSQSS QSSQRYSR

Length:628

Mass (Da):64,417

Last modified:November 30, 2010 - v3

Checksum:ⁱ65FDB8CAA7F3C960

GO

Sequence cautionⁱ

The sequence CAF31522 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	162	G → A in CAA28991 (PubMed:2439698).Curated	1
Sequence conflictⁱ	175	I → T in CAA28991 (PubMed:2439698).Curated	1
Sequence conflictⁱ	184	N → K in CAA28991 (PubMed:2439698).Curated	1
Sequence conflictⁱ	187	I → T in CAA28991 (PubMed:2439698).Curated	1
Sequence conflictⁱ	298	T → Y in CAA28993 (PubMed:2439698).Curated	1
Sequence conflictⁱ	397	L → M in CAA28996 (PubMed:2439698).Curated	1
Sequence conflictⁱ	409	D → G in CAF31522 (PubMed:15737194).Curated	1
Sequence conflictⁱ	448	E → Q in CAA28996 (PubMed:2439698).Curated	1
Sequence conflictⁱ	511	Y → YS in CAA28996 (PubMed:2439698).Curated	1
Sequence conflictⁱ	562	S → I in CAA28996 (PubMed:2439698).Curated	1
Sequence conflictⁱ	580	S → T in CAA28996 (PubMed:2439698).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_061297	44	G → A. Corresponds to variant dbSNP:rs28721426Ensembl.	1
Natural variantⁱVAR_056023	375	R → G1 PublicationCorresponds to variant dbSNP:rs3887954Ensembl.	1
Natural variantⁱVAR_031327	503	R → P in MECD. 1 PublicationCorresponds to variant dbSNP:rs60410063EnsemblClinVar.	1
Natural variantⁱVAR_003868	509	E → K in MECD. 1 PublicationCorresponds to variant dbSNP:rs57872071EnsemblClinVar.	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X05418 Genomic DNA Translation: CAA28991.1 X05419 Genomic DNA Translation: CAA28992.1 X05420 Genomic DNA Translation: CAA28993.1 X05420 Genomic DNA Translation: CAA28994.1 X05420 Genomic DNA Translation: CAA28995.1 X05421 Genomic DNA Translation: CAA28996.1 AC107016 Genomic DNA No translation available. AJ628418 mRNA Translation: CAF31522.1 Different initiation.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS44895.1
PIRⁱ	A29666
NCBI Reference Sequences More...RefSeqⁱ	NP_476429.2, NM_057088.2
UniGeneⁱ	Hs.680652

Genome annotation databases

Ensemblⁱ	ENST00000417996; ENSP00000413479; ENSG00000186442
GeneIDⁱ	3850
KEGGⁱ	hsa:3850
UCSC genome browser More...UCSCⁱ	uc001say.4 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Protein	Similar proteins		Species	Score	Length	Source
P12035	Uncharacterized protein		CHLSB		643	UniRef90_P12035
Uncharacterized protein		CERAT		633
KRT3 isoform 1		PANTR		629
Uncharacterized protein		RHIRO		629
Uncharacterized protein		AOTNA		629
+29

Protein	Similar proteins		Species	Score	Length	Source
P12035	Uncharacterized protein		CHLSB		643	UniRef50_P12035
Uncharacterized protein		CERAT		633
Uncharacterized protein		MANLE		629
Uncharacterized protein		AOTNA		629
Uncharacterized protein		RHIBE		629
+62

Cross-referencesⁱ

Web resourcesⁱ

Human Intermediate Filament Mutation Database

Wikipedia

Keratin-3 entry

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X05418 Genomic DNA Translation: CAA28991.1 X05419 Genomic DNA Translation: CAA28992.1 X05420 Genomic DNA Translation: CAA28993.1 X05420 Genomic DNA Translation: CAA28994.1 X05420 Genomic DNA Translation: CAA28995.1 X05421 Genomic DNA Translation: CAA28996.1 AC107016 Genomic DNA No translation available. AJ628418 mRNA Translation: CAF31522.1 Different initiation.
CCDSⁱ	CCDS44895.1
PIRⁱ	A29666
RefSeqⁱ	NP_476429.2, NM_057088.2
UniGeneⁱ	Hs.680652

3D structure databases

ProteinModelPortalⁱ	P12035
SMRⁱ	P12035
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	110048, 18 interactors
IntActⁱ	P12035, 45 interactors
STRINGⁱ	9606.ENSP00000413479

PTM databases

iPTMnetⁱ	P12035
PhosphoSitePlusⁱ	P12035
SwissPalmⁱ	P12035

Polymorphism and mutation databases

BioMutaⁱ	KRT3
DMDMⁱ	313104225

Proteomic databases

PaxDbⁱ	P12035
PeptideAtlasⁱ	P12035
PRIDEⁱ	P12035
ProteomicsDBⁱ	52820

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	3850
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000417996; ENSP00000413479; ENSG00000186442
GeneIDⁱ	3850
KEGGⁱ	hsa:3850
UCSCⁱ	uc001say.4 human

Organism-specific databases

CTDⁱ	3850
DisGeNETⁱ	3850
EuPathDBⁱ	HostDB:ENSG00000186442.7
GeneCardsⁱ	KRT3
H-InvDBⁱ	HIX0036742
HGNCⁱ	HGNC:6440 KRT3
MalaCardsⁱ	KRT3
MIMⁱ	122100 phenotype 148043 gene
neXtProtⁱ	NX_P12035
OpenTargetsⁱ	ENSG00000186442
Orphanetⁱ	98954 Meesmann corneal dystrophy
PharmGKBⁱ	PA30228
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IKBD Eukaryota ENOG4111UGN LUCA
GeneTreeⁱ	ENSGT00760000118796
HOGENOMⁱ	HOG000230976
HOVERGENⁱ	HBG013015
InParanoidⁱ	P12035
KOⁱ	K07605
OMAⁱ	DSEMRNM
OrthoDBⁱ	EOG091G09KR
PhylomeDBⁱ	P12035
TreeFamⁱ	TF332742

Enzyme and pathway databases

Reactomeⁱ	R-HSA-6805567 Keratinization R-HSA-6809371 Formation of the cornified envelope

Reactomeⁱ

R-HSA-6805567 Keratinization
R-HSA-6809371 Formation of the cornified envelope

Miscellaneous databases

GeneWikiⁱ	Keratin_3
GenomeRNAiⁱ	3850
Protein Ontology More...PROⁱ	PR:P12035
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000186442 Expressed in 27 organ(s), highest expression level in gingiva
CleanExⁱ	HS_KRT3
Genevisibleⁱ	P12035 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR001664 IF IPR018039 IF_conserved IPR039008 IF_rod_dom IPR032444 Keratin_2_head IPR003054 Keratin_II
PANTHERⁱ	PTHR23239 PTHR23239, 2 hits
Pfamⁱ	View protein in Pfam PF00038 Filament, 1 hit PF16208 Keratin_2_head, 2 hits
PRINTSⁱ	PR01276 TYPE2KERATIN
SMARTⁱ	View protein in SMART SM01391 Filament, 1 hit
PROSITEⁱ	View protein in PROSITE PS00226 IF_ROD_1, 1 hit PS51842 IF_ROD_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	K2C3_HUMAN
Accessionⁱ	P12035Primary (citable) accession number: P12035 Secondary accession number(s): A6NIS2, Q701L8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
	Last sequence update:	November 30, 2010
	Last modified:	November 7, 2018
	This is version 176 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM

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UniProtKB - P12035 (K2C3_HUMAN)

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Keratin, type II cytoskeletal 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytoskeleton

Cytosol

Extracellular region or secreted

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Keywords - Diseasei

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ