UniProtKB - P12008 (AROC_ECOLI)
Protein
Chorismate synthase
Gene
aroC
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system. It uses NADPH to reduce FMN.UniRule annotation6 Publications
Catalytic activityi
- EC:4.2.3.5UniRule annotation5 Publications
Cofactori
FMNH2UniRule annotation6 PublicationsNote: Reduced FMN (FMNH(2)). It can also use FAD, however FMN is the preferred cofactor.UniRule annotation6 Publications
Activity regulationi
Competitively inhibited by 6R-6-fluoro-EPSP.2 Publications
Kineticsi
kcat is 16.5 sec(-1) for phospholyase activity with EPSP as substrate. kcat is 27 sec(-1) for phospholyase activity with EPSP as substrate.2 Publications
- KM=1.3 µM for EPSP1 Publication
- Vmax=25 µmol/min/mg enzyme1 Publication
pH dependencei
Optimum pH is between 7.5 and 8.1 Publication
: chorismate biosynthesis Pathwayi
This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotationProteins known to be involved in the 7 steps of the subpathway in this organism are:
- Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (aroG)
- 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
- 3-dehydroquinate dehydratase (aroD), 3-dehydroquinate dehydratase (aroD)
- Quinate/shikimate dehydrogenase (ydiB), Quinate/shikimate dehydrogenase (ydiB), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE)
- Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK), Shikimate kinase 1 (aroK), Shikimate kinase 2 (aroL)
- 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
- Chorismate synthase (aroC), Chorismate synthase (aroC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 48 | NADPUniRule annotation | 1 | |
Binding sitei | 54 | NADPUniRule annotation | 1 | |
Binding sitei | 278 | FMN; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 319 | FMNUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 125 – 127 | FMNUniRule annotation | 3 | |
Nucleotide bindingi | 238 – 239 | FMNUniRule annotation | 2 | |
Nucleotide bindingi | 293 – 297 | FMNUniRule annotation | 5 |
GO - Molecular functioni
- chorismate synthase activity Source: EcoCyc
- FMN binding Source: UniProtKB
- identical protein binding Source: EcoCyc
GO - Biological processi
- aromatic amino acid family biosynthetic process Source: GO_Central
- cellular amino acid biosynthetic process Source: UniProtKB-KW
- chorismate biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Aromatic amino acid biosynthesis |
Ligand | FAD, Flavoprotein, FMN, NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:AROC-MONOMER MetaCyc:AROC-MONOMER |
UniPathwayi | UPA00053;UER00090 |
Names & Taxonomyi
Protein namesi | Recommended name: Chorismate synthase1 PublicationUniRule annotation (EC:4.2.3.5UniRule annotation5 Publications)Short name: CSUniRule annotation Alternative name(s): 5-enolpyruvylshikimate-3-phosphate phospholyase1 PublicationUniRule annotation Short name: EPSP phospholyase1 Publication |
Gene namesi | Name:aroC1 PublicationUniRule annotation Ordered Locus Names:b2329, JW2326 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000140584 | 2 – 361 | Chorismate synthaseAdd BLAST | 360 |
Proteomic databases
jPOSTi | P12008 |
PaxDbi | P12008 |
PRIDEi | P12008 |
Interactioni
Subunit structurei
Homotetramer.
UniRule annotation2 PublicationsGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4263044, 43 interactors |
IntActi | P12008, 5 interactors |
STRINGi | 511145.b2329 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0082, Bacteria |
InParanoidi | P12008 |
PhylomeDBi | P12008 |
Family and domain databases
CDDi | cd07304, Chorismate_synthase, 1 hit |
Gene3Di | 3.60.150.10, 1 hit |
HAMAPi | MF_00300, Chorismate_synth, 1 hit |
InterProi | View protein in InterPro IPR000453, Chorismate_synth IPR035904, Chorismate_synth_AroC_sf IPR020541, Chorismate_synthase_CS |
PANTHERi | PTHR21085, PTHR21085, 1 hit |
Pfami | View protein in Pfam PF01264, Chorismate_synt, 1 hit |
PIRSFi | PIRSF001456, Chorismate_synth, 1 hit |
SUPFAMi | SSF103263, SSF103263, 1 hit |
TIGRFAMsi | TIGR00033, aroC, 1 hit |
PROSITEi | View protein in PROSITE PS00787, CHORISMATE_SYNTHASE_1, 1 hit PS00788, CHORISMATE_SYNTHASE_2, 1 hit PS00789, CHORISMATE_SYNTHASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P12008-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGNTIGQLF RVTTFGESHG LALGCIVDGV PPGIPLTEAD LQHDLDRRRP
60 70 80 90 100
GTSRYTTQRR EPDQVKILSG VFEGVTTGTS IGLLIENTDQ RSQDYSAIKD
110 120 130 140 150
VFRPGHADYT YEQKYGLRDY RGGGRSSARE TAMRVAAGAI AKKYLAEKFG
160 170 180 190 200
IEIRGCLTQM GDIPLDIKDW SQVEQNPFFC PDPDKIDALD ELMRALKKEG
210 220 230 240 250
DSIGAKVTVV ASGVPAGLGE PVFDRLDADI AHALMSINAV KGVEIGDGFD
260 270 280 290 300
VVALRGSQNR DEITKDGFQS NHAGGILGGI SSGQQIIAHM ALKPTSSITV
310 320 330 340 350
PGRTINRFGE EVEMITKGRH DPCVGIRAVP IAEAMLAIVL MDHLLRQRAQ
360
NADVKTDIPR W
Sequence cautioni
The sequence AAA23488 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27714 Genomic DNA Translation: AAA23487.1 Y00720 Genomic DNA Translation: CAA68707.1 Frameshift. M33021 Genomic DNA Translation: AAA23488.1 Frameshift. U00096 Genomic DNA Translation: AAC75389.1 AP009048 Genomic DNA Translation: BAA16185.1 |
PIRi | G65005, SYECKR |
RefSeqi | NP_416832.1, NC_000913.3 WP_001333535.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75389; AAC75389; b2329 BAA16185; BAA16185; BAA16185 |
GeneIDi | 57728065 946814 |
KEGGi | ecj:JW2326 eco:b2329 |
PATRICi | fig|511145.12.peg.2425 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27714 Genomic DNA Translation: AAA23487.1 Y00720 Genomic DNA Translation: CAA68707.1 Frameshift. M33021 Genomic DNA Translation: AAA23488.1 Frameshift. U00096 Genomic DNA Translation: AAC75389.1 AP009048 Genomic DNA Translation: BAA16185.1 |
PIRi | G65005, SYECKR |
RefSeqi | NP_416832.1, NC_000913.3 WP_001333535.1, NZ_LN832404.1 |
3D structure databases
SMRi | P12008 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263044, 43 interactors |
IntActi | P12008, 5 interactors |
STRINGi | 511145.b2329 |
Proteomic databases
jPOSTi | P12008 |
PaxDbi | P12008 |
PRIDEi | P12008 |
Genome annotation databases
EnsemblBacteriai | AAC75389; AAC75389; b2329 BAA16185; BAA16185; BAA16185 |
GeneIDi | 57728065 946814 |
KEGGi | ecj:JW2326 eco:b2329 |
PATRICi | fig|511145.12.peg.2425 |
Organism-specific databases
EchoBASEi | EB0073 |
Phylogenomic databases
eggNOGi | COG0082, Bacteria |
InParanoidi | P12008 |
PhylomeDBi | P12008 |
Enzyme and pathway databases
UniPathwayi | UPA00053;UER00090 |
BioCyci | EcoCyc:AROC-MONOMER MetaCyc:AROC-MONOMER |
Miscellaneous databases
PROi | PR:P12008 |
Family and domain databases
CDDi | cd07304, Chorismate_synthase, 1 hit |
Gene3Di | 3.60.150.10, 1 hit |
HAMAPi | MF_00300, Chorismate_synth, 1 hit |
InterProi | View protein in InterPro IPR000453, Chorismate_synth IPR035904, Chorismate_synth_AroC_sf IPR020541, Chorismate_synthase_CS |
PANTHERi | PTHR21085, PTHR21085, 1 hit |
Pfami | View protein in Pfam PF01264, Chorismate_synt, 1 hit |
PIRSFi | PIRSF001456, Chorismate_synth, 1 hit |
SUPFAMi | SSF103263, SSF103263, 1 hit |
TIGRFAMsi | TIGR00033, aroC, 1 hit |
PROSITEi | View protein in PROSITE PS00787, CHORISMATE_SYNTHASE_1, 1 hit PS00788, CHORISMATE_SYNTHASE_2, 1 hit PS00789, CHORISMATE_SYNTHASE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AROC_ECOLI | |
Accessioni | P12008Primary (citable) accession number: P12008 Secondary accession number(s): P78193 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 170 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families