UniProtKB - P11988 (BGLB_ECOLI)
Protein
6-phospho-beta-glucosidase BglB
Gene
bglB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the hydrolysis of phosphorylated beta-glucosides into glucose-6-phosphate (G-6-P) and aglycone. It has a high affinity for phosphorylated aromatic beta-glucosides (p-nitrophenyl-beta-glucoside, phenyl beta-glucoside, arbutin and phosphorylated salicin), and a low affinity for phosphorylated beta-methyl-glucoside.1 Publication
Miscellaneous
Part of the cryptic bglGFBH operon.
Catalytic activityi
- EC:3.2.1.86
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 172 | Proton donorSequence analysis | 1 | |
Active sitei | 361 | NucleophilePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 6-phospho-beta-glucosidase activity Source: EcoCyc
- beta-glucosidase activity Source: GO_Central
- hydrolase activity, hydrolyzing O-glycosyl compounds Source: UniProtKB
- methyl beta-D-glucoside 6-phosphate glucohydrolase activity Source: UniProtKB-EC
GO - Biological processi
- carbohydrate catabolic process Source: GO_Central
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10114-MONOMER MetaCyc:EG10114-MONOMER |
SABIO-RKi | P11988 |
Protein family/group databases
CAZyi | GH1, Glycoside Hydrolase Family 1 |
Names & Taxonomyi
Protein namesi | Recommended name: 6-phospho-beta-glucosidase BglB (EC:3.2.1.86)Alternative name(s): Phospho-beta-glucosidase B |
Gene namesi | Name:bglB Ordered Locus Names:b3721, JW3699 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063898 | 1 – 470 | 6-phospho-beta-glucosidase BglBAdd BLAST | 470 |
Proteomic databases
PaxDbi | P11988 |
PRIDEi | P11988 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261195, 13 interactors 852536, 1 interactor |
DIPi | DIP-9214N |
IntActi | P11988, 4 interactors |
STRINGi | 511145.b3721 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 1 family.Curated
Phylogenomic databases
eggNOGi | COG2723, Bacteria |
HOGENOMi | CLU_001859_0_2_6 |
InParanoidi | P11988 |
PhylomeDBi | P11988 |
Family and domain databases
InterProi | View protein in InterPro IPR001360, Glyco_hydro_1 IPR018120, Glyco_hydro_1_AS IPR033132, Glyco_hydro_1_N_CS IPR017853, Glycoside_hydrolase_SF |
PANTHERi | PTHR10353, PTHR10353, 1 hit |
Pfami | View protein in Pfam PF00232, Glyco_hydro_1, 1 hit |
PRINTSi | PR00131, GLHYDRLASE1 |
SUPFAMi | SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00572, GLYCOSYL_HYDROL_F1_1, 1 hit PS00653, GLYCOSYL_HYDROL_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P11988-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKAFPETFLW GGATAANQVE GAWQEDGKGI STSDLQPHGV MGKMEPRILG
60 70 80 90 100
KENIKDVAID FYHRYPEDIA LFAEMGFTCL RISIAWARIF PQGDEVEPNE
110 120 130 140 150
AGLAFYDRLF DEMAQAGIKP LVTLSHYEMP YGLVKNYGGW ANRAVIDHFE
160 170 180 190 200
HYARTVFTRY QHKVALWLTF NEINMSLHAP FTGVGLAEES GEAEVYQAIH
210 220 230 240 250
HQLVASARAV KACHSLLPEA KIGNMLLGGL VYPLTCQPQD MLQAMEENRR
260 270 280 290 300
WMFFGDVQAR GQYPGYMQRF FRDHNITIEM TESDAEDLKH TVDFISFSYY
310 320 330 340 350
MTGCVSHDES INKNAQGNIL NMIPNPHLKS SEWGWQIDPV GLRVLLNTLW
360 370 380 390 400
DRYQKPLFIV ENGLGAKDSV EADGSIQDDY RIAYLNDHLV QVNEAIADGV
410 420 430 440 450
DIMGYTSWGP IDLVSASHSQ MSKRYGFIYV DRDDNGEGSL TRTRKKSFGW
460 470
YAEVIKTRGL SLKKITIKAP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 147 | D → G in AAA23511 (PubMed:3034860).Curated | 1 | |
Sequence conflicti | 449 – 451 | GWY → RMVC in AAA23511 (PubMed:3034860).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16487 Genomic DNA Translation: AAA23511.1 L10328 Genomic DNA Translation: AAA62072.1 U00096 Genomic DNA Translation: AAC76744.1 AP009048 Genomic DNA Translation: BAE77567.1 M15746 Genomic DNA Translation: AAA83838.1 |
PIRi | B65175 |
RefSeqi | NP_418177.1, NC_000913.3 WP_000643228.1, NZ_SSZK01000036.1 |
Genome annotation databases
EnsemblBacteriai | AAC76744; AAC76744; b3721 BAE77567; BAE77567; BAE77567 |
GeneIDi | 57729039 948234 |
KEGGi | ecj:JW3699 eco:b3721 |
PATRICi | fig|1411691.4.peg.2980 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16487 Genomic DNA Translation: AAA23511.1 L10328 Genomic DNA Translation: AAA62072.1 U00096 Genomic DNA Translation: AAC76744.1 AP009048 Genomic DNA Translation: BAE77567.1 M15746 Genomic DNA Translation: AAA83838.1 |
PIRi | B65175 |
RefSeqi | NP_418177.1, NC_000913.3 WP_000643228.1, NZ_SSZK01000036.1 |
3D structure databases
SMRi | P11988 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261195, 13 interactors 852536, 1 interactor |
DIPi | DIP-9214N |
IntActi | P11988, 4 interactors |
STRINGi | 511145.b3721 |
Protein family/group databases
CAZyi | GH1, Glycoside Hydrolase Family 1 |
Proteomic databases
PaxDbi | P11988 |
PRIDEi | P11988 |
Genome annotation databases
EnsemblBacteriai | AAC76744; AAC76744; b3721 BAE77567; BAE77567; BAE77567 |
GeneIDi | 57729039 948234 |
KEGGi | ecj:JW3699 eco:b3721 |
PATRICi | fig|1411691.4.peg.2980 |
Organism-specific databases
EchoBASEi | EB0112 |
Phylogenomic databases
eggNOGi | COG2723, Bacteria |
HOGENOMi | CLU_001859_0_2_6 |
InParanoidi | P11988 |
PhylomeDBi | P11988 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10114-MONOMER MetaCyc:EG10114-MONOMER |
SABIO-RKi | P11988 |
Miscellaneous databases
PROi | PR:P11988 |
Family and domain databases
InterProi | View protein in InterPro IPR001360, Glyco_hydro_1 IPR018120, Glyco_hydro_1_AS IPR033132, Glyco_hydro_1_N_CS IPR017853, Glycoside_hydrolase_SF |
PANTHERi | PTHR10353, PTHR10353, 1 hit |
Pfami | View protein in Pfam PF00232, Glyco_hydro_1, 1 hit |
PRINTSi | PR00131, GLHYDRLASE1 |
SUPFAMi | SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00572, GLYCOSYL_HYDROL_F1_1, 1 hit PS00653, GLYCOSYL_HYDROL_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BGLB_ECOLI | |
Accessioni | P11988Primary (citable) accession number: P11988 Secondary accession number(s): P78124, Q2M839 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | July 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families