Cd3g

Functionⁱ

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G (PubMed:25920998).By similarity2 Publications

GO - Molecular functionⁱ

protein heterodimerization activity Source: UniProtKB
protein homodimerization activity Source: MGI
transmembrane signaling receptor activity Source: UniProtKB

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adaptive immune response Source: UniProtKB-KW
cell surface receptor signaling pathway Source: UniProtKB
establishment or maintenance of cell polarity Source: UniProtKB
positive thymic T cell selection
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein homooligomerization Source: MGI
protein transport Source: UniProtKB
regulation of lymphocyte apoptotic process Source: UniProtKB
T cell differentiation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Receptor
Biological process	Adaptive immunity, Immunity

Enzyme and pathway databases

Reactomeⁱ	R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-202424 Downstream TCR signaling R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains R-MMU-202430 Translocation of ZAP-70 to Immunological synapse R-MMU-202433 Generation of second messenger molecules R-MMU-2029481 FCGR activation R-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation R-MMU-2029485 Role of phospholipids in phagocytosis R-MMU-389948 PD-1 signaling R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828 Clathrin-mediated endocytosis

Reactomeⁱ

R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-202424 Downstream TCR signaling
R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains
R-MMU-202430 Translocation of ZAP-70 to Immunological synapse
R-MMU-202433 Generation of second messenger molecules
R-MMU-2029481 FCGR activation
R-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-2029485 Role of phospholipids in phagocytosis
R-MMU-389948 PD-1 signaling
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: T-cell surface glycoprotein CD3 gamma chain Alternative name(s): T-cell receptor T3 gamma chain CD_antigen: CD3g
Gene namesⁱ	Name:Cd3g
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 9

Organism-specific databases

MGIⁱ	MGI:88333 Cd3g

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	23 – 116	ExtracellularSequence analysisAdd BLAST	94
Transmembraneⁱ	117 – 137	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	138 – 182	CytoplasmicSequence analysisAdd BLAST	45

Keywords - Cellular componentⁱ

Membrane

Pathology & Biotechⁱ

Disruption phenotypeⁱ

The absence of CD3G results in a severe reduction in the level of the TCR-CD3 at the cell surface of thymocytes and peripheral T cells. The development of both the TCRalphabeta and TCRgammadelta lineages are affected by the absence of CD3G.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 22	Add BLAST		22
ChainⁱPRO_0000014617	23 – 182	T-cell surface glycoprotein CD3 gamma chainAdd BLAST		160

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	46 ↔ 87	By similarity
Glycosylationⁱ	66	N-linked (GlcNAc...) asparagineSequence analysis	1
Modified residueⁱ	145	PhosphoserineBy similarity	1
Modified residueⁱ	148	PhosphoserineBy similarity	1

Post-translational modificationⁱ

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. Phosphorylated also by PKC; leading to the TCR complex down-regulation.By similarity

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDⁱ	P11942
PaxDbⁱ	P11942
PRIDEⁱ	P11942

PTM databases

iPTMnetⁱ	P11942
PhosphoSitePlusⁱ	P11942

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000002033 Expressed in 102 organ(s), highest expression level in thymus
CleanExⁱ	MM_CD3G
ExpressionAtlasⁱ	P11942 baseline and differential
Genevisibleⁱ	P11942 MM

Interactionⁱ

Subunit structureⁱ

The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta.By similarity

GO - Molecular functionⁱ

protein heterodimerization activity Source: UniProtKB
protein homodimerization activity Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

CORUMⁱ	P11942
ELMⁱ	P11942
IntActⁱ	P11942, 1 interactor
STRINGⁱ	10090.ENSMUSP00000002101

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	37 – 39	Combined sources	3
Beta strandⁱ	41 – 45	Combined sources	5
Beta strandⁱ	47 – 49	Combined sources	3
Beta strandⁱ	53 – 57	Combined sources	5
Beta strandⁱ	73 – 76	Combined sources	4
Beta strandⁱ	82 – 91	Combined sources	10
Beta strandⁱ	93 – 100	Combined sources	8

Show more details

3D structure databases

ProteinModelPortalⁱ	P11942
SMRⁱ	P11942
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P11942

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	37 – 94	Ig-likeAdd BLAST		58
Domainⁱ	149 – 177	ITAMPROSITE-ProRule annotationAdd BLAST		29

Keywords - Domainⁱ

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IWAX Eukaryota ENOG410Y2TD LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000046930
HOGENOMⁱ	HOG000015287
HOVERGENⁱ	HBG005278
InParanoidⁱ	P11942
KEGG Orthology (KO) More...KOⁱ	K06452
OMAⁱ	QNCIELS
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0MXQ
PhylomeDBⁱ	P11942
TreeFamⁱ	TF335892

Family and domain databases

Gene3Dⁱ	2.60.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR015484 CD3_esu/gsu/dsu IPR036179 Ig-like_dom_sf IPR013783 Ig-like_fold IPR032052 Ig_4 IPR003598 Ig_sub2 IPR003110 Phos_immunorcpt_sig_ITAM
PANTHERⁱ	PTHR10570 PTHR10570, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF16680 Ig_4, 1 hit PF02189 ITAM, 1 hit
SMARTⁱ	View protein in SMART SM00408 IGc2, 1 hit SM00077 ITAM, 1 hit
SUPFAMⁱ	SSF48726 SSF48726, 1 hit
PROSITEⁱ	View protein in PROSITE PS51055 ITAM_1, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

P11942-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEQRKGLAGL FLVISLLQGT VAQTNKAKNL VQVDGSRGDG SVLLTCGLTD 
        60         70         80         90        100
KTIKWLKDGS IISPLNATKN TWNLGNNAKD PRGTYQCQGA KETSNPLQVY 
       110        120        130        140        150
YRMCENCIEL NIGTISGFIF AEVISIFFLA LGVYLIAGQD GVRQSRASDK 
       160        170        180 
QTLLQNEQLY QPLKDREYDQ YSHLQGNQLR KK

Length:182

Mass (Da):20,234

Last modified:October 1, 1989 - v1

Checksum:ⁱ7F9D8D44A5C360D5

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	E0CXP3	E0CXP3_MOUSE		T-cell surface glycoprotein CD3 gam... T-cell surface glycoprotein CD3 gamma chain	Cd3g	100	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Y00635 mRNA Translation: CAA68667.1 M58149 mRNA Translation: AAA63289.1 Sequence problems. M18227 M18226 Genomic DNA Translation: AAA37400.1 BC027528 mRNA Translation: AAH27528.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS23123.1
PIRⁱ	A39952 A28508
NCBI Reference Sequences More...RefSeqⁱ	NP_033980.1, NM_009850.2
UniGeneⁱ	Mm.335106

Genome annotation databases

Ensemblⁱ	ENSMUST00000002101; ENSMUSP00000002101; ENSMUSG00000002033
GeneIDⁱ	12502
KEGGⁱ	mmu:12502
UCSC genome browser More...UCSCⁱ	uc009pex.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P11942	CD3 antigen gamma polypeptide		MOUSE		182	UniRef100_P11942

Protein	Similar proteins		Species	Score	Length	Source
P11942	CD3 antigen gamma polypeptide		MOUSE		182	UniRef90_P11942
UPI000A30AFC5		MUSPA		182
UPI000A318529		MUSCR		182
Uncharacterized protein		MOUSE		182

Protein	Similar proteins		Species	Score	Length	Source
P11942	T-cell surface glycoprotein CD3 gamma chain		RAT		182	UniRef50_P11942
T-cell surface glycoprotein CD3 gamma chain		PIG		182
T-cell surface glycoprotein CD3 gamma chain		BOVIN		173
CD3 antigen gamma polypeptide		MOUSE		182
T-cell surface glycoprotein CD3 gamma chain		CASCN		183
+112

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Y00635 mRNA Translation: CAA68667.1 M58149 mRNA Translation: AAA63289.1 Sequence problems. M18227 M18226 Genomic DNA Translation: AAA37400.1 BC027528 mRNA Translation: AAH27528.1
CCDSⁱ	CCDS23123.1
PIRⁱ	A39952 A28508
RefSeqⁱ	NP_033980.1, NM_009850.2
UniGeneⁱ	Mm.335106

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1JBJ	NMR	-	A	23-103	[»]
ProteinModelPortalⁱ	P11942
SMRⁱ	P11942
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

CORUMⁱ	P11942
ELMⁱ	P11942
IntActⁱ	P11942, 1 interactor
STRINGⁱ	10090.ENSMUSP00000002101

PTM databases

iPTMnetⁱ	P11942
PhosphoSitePlusⁱ	P11942

Proteomic databases

EPDⁱ	P11942
PaxDbⁱ	P11942
PRIDEⁱ	P11942

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000002101; ENSMUSP00000002101; ENSMUSG00000002033
GeneIDⁱ	12502
KEGGⁱ	mmu:12502
UCSCⁱ	uc009pex.2 mouse

Organism-specific databases

CTDⁱ	917
MGIⁱ	MGI:88333 Cd3g

Phylogenomic databases

eggNOGⁱ	ENOG410IWAX Eukaryota ENOG410Y2TD LUCA
GeneTreeⁱ	ENSGT00510000046930
HOGENOMⁱ	HOG000015287
HOVERGENⁱ	HBG005278
InParanoidⁱ	P11942
KOⁱ	K06452
OMAⁱ	QNCIELS
OrthoDBⁱ	EOG091G0MXQ
PhylomeDBⁱ	P11942
TreeFamⁱ	TF335892

Enzyme and pathway databases

Reactomeⁱ	R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-202424 Downstream TCR signaling R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains R-MMU-202430 Translocation of ZAP-70 to Immunological synapse R-MMU-202433 Generation of second messenger molecules R-MMU-2029481 FCGR activation R-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation R-MMU-2029485 Role of phospholipids in phagocytosis R-MMU-389948 PD-1 signaling R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828 Clathrin-mediated endocytosis

Reactomeⁱ

R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-202424 Downstream TCR signaling
R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains
R-MMU-202430 Translocation of ZAP-70 to Immunological synapse
R-MMU-202433 Generation of second messenger molecules
R-MMU-2029481 FCGR activation
R-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-2029485 Role of phospholipids in phagocytosis
R-MMU-389948 PD-1 signaling
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

EvolutionaryTraceⁱ	P11942
Protein Ontology More...PROⁱ	PR:P11942
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000002033 Expressed in 102 organ(s), highest expression level in thymus
CleanExⁱ	MM_CD3G
ExpressionAtlasⁱ	P11942 baseline and differential
Genevisibleⁱ	P11942 MM

Family and domain databases

Gene3Dⁱ	2.60.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR015484 CD3_esu/gsu/dsu IPR036179 Ig-like_dom_sf IPR013783 Ig-like_fold IPR032052 Ig_4 IPR003598 Ig_sub2 IPR003110 Phos_immunorcpt_sig_ITAM
PANTHERⁱ	PTHR10570 PTHR10570, 1 hit
Pfamⁱ	View protein in Pfam PF16680 Ig_4, 1 hit PF02189 ITAM, 1 hit
SMARTⁱ	View protein in SMART SM00408 IGc2, 1 hit SM00077 ITAM, 1 hit
SUPFAMⁱ	SSF48726 SSF48726, 1 hit
PROSITEⁱ	View protein in PROSITE PS51055 ITAM_1, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CD3G_MOUSE
Accessionⁱ	P11942Primary (citable) accession number: P11942
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
	Last sequence update:	October 1, 1989
	Last modified:	November 7, 2018
	This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniRef

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Supporting data

UniProtKB - P11942 (CD3G_MOUSE)

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T-cell surface glycoprotein CD3 gamma chain

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ