Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

T-cell surface glycoprotein CD3 gamma chain

Gene

Cd3g

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G (PubMed:25920998).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-202424 Downstream TCR signaling
R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains
R-MMU-202430 Translocation of ZAP-70 to Immunological synapse
R-MMU-202433 Generation of second messenger molecules
R-MMU-2029481 FCGR activation
R-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-2029485 Role of phospholipids in phagocytosis
R-MMU-389948 PD-1 signaling
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD3 gamma chain
Alternative name(s):
T-cell receptor T3 gamma chain
CD_antigen: CD3g
Gene namesi
Name:Cd3g
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:88333 Cd3g

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 116ExtracellularSequence analysisAdd BLAST94
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Topological domaini138 – 182CytoplasmicSequence analysisAdd BLAST45

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

The absence of CD3G results in a severe reduction in the level of the TCR-CD3 at the cell surface of thymocytes and peripheral T cells. The development of both the TCRalphabeta and TCRgammadelta lineages are affected by the absence of CD3G.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000001461723 – 182T-cell surface glycoprotein CD3 gamma chainAdd BLAST160

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi46 ↔ 87By similarity
Glycosylationi66N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei145PhosphoserineBy similarity1
Modified residuei148PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. Phosphorylated also by PKC; leading to the TCR complex down-regulation.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP11942
PaxDbiP11942
PRIDEiP11942

PTM databases

iPTMnetiP11942
PhosphoSitePlusiP11942

Expressioni

Gene expression databases

BgeeiENSMUSG00000002033 Expressed in 102 organ(s), highest expression level in thymus
CleanExiMM_CD3G
ExpressionAtlasiP11942 baseline and differential
GenevisibleiP11942 MM

Interactioni

Subunit structurei

The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta.By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP11942
ELMiP11942
IntActiP11942, 1 interactor
STRINGi10090.ENSMUSP00000002101

Structurei

Secondary structure

1182
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP11942
SMRiP11942
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11942

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 94Ig-likeAdd BLAST58
Domaini149 – 177ITAMPROSITE-ProRule annotationAdd BLAST29

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IWAX Eukaryota
ENOG410Y2TD LUCA
GeneTreeiENSGT00510000046930
HOGENOMiHOG000015287
HOVERGENiHBG005278
InParanoidiP11942
KOiK06452
OMAiQNCIELS
OrthoDBiEOG091G0MXQ
PhylomeDBiP11942
TreeFamiTF335892

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015484 CD3_esu/gsu/dsu
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR032052 Ig_4
IPR003598 Ig_sub2
IPR003110 Phos_immunorcpt_sig_ITAM
PANTHERiPTHR10570 PTHR10570, 1 hit
PfamiView protein in Pfam
PF16680 Ig_4, 1 hit
PF02189 ITAM, 1 hit
SMARTiView protein in SMART
SM00408 IGc2, 1 hit
SM00077 ITAM, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS51055 ITAM_1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P11942-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEQRKGLAGL FLVISLLQGT VAQTNKAKNL VQVDGSRGDG SVLLTCGLTD
60 70 80 90 100
KTIKWLKDGS IISPLNATKN TWNLGNNAKD PRGTYQCQGA KETSNPLQVY
110 120 130 140 150
YRMCENCIEL NIGTISGFIF AEVISIFFLA LGVYLIAGQD GVRQSRASDK
160 170 180
QTLLQNEQLY QPLKDREYDQ YSHLQGNQLR KK
Length:182
Mass (Da):20,234
Last modified:October 1, 1989 - v1
Checksum:i7F9D8D44A5C360D5
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E0CXP3E0CXP3_MOUSE
T-cell surface glycoprotein CD3 gam...
Cd3g
100Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00635 mRNA Translation: CAA68667.1
M58149 mRNA Translation: AAA63289.1 Sequence problems.
M18227
, M18222, M18223, M18224, M18225, M18226 Genomic DNA Translation: AAA37400.1
BC027528 mRNA Translation: AAH27528.1
CCDSiCCDS23123.1
PIRiA39952 A28508
RefSeqiNP_033980.1, NM_009850.2
UniGeneiMm.335106

Genome annotation databases

EnsembliENSMUST00000002101; ENSMUSP00000002101; ENSMUSG00000002033
GeneIDi12502
KEGGimmu:12502
UCSCiuc009pex.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00635 mRNA Translation: CAA68667.1
M58149 mRNA Translation: AAA63289.1 Sequence problems.
M18227
, M18222, M18223, M18224, M18225, M18226 Genomic DNA Translation: AAA37400.1
BC027528 mRNA Translation: AAH27528.1
CCDSiCCDS23123.1
PIRiA39952 A28508
RefSeqiNP_033980.1, NM_009850.2
UniGeneiMm.335106

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JBJNMR-A23-103[»]
ProteinModelPortaliP11942
SMRiP11942
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiP11942
ELMiP11942
IntActiP11942, 1 interactor
STRINGi10090.ENSMUSP00000002101

PTM databases

iPTMnetiP11942
PhosphoSitePlusiP11942

Proteomic databases

EPDiP11942
PaxDbiP11942
PRIDEiP11942

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002101; ENSMUSP00000002101; ENSMUSG00000002033
GeneIDi12502
KEGGimmu:12502
UCSCiuc009pex.2 mouse

Organism-specific databases

CTDi917
MGIiMGI:88333 Cd3g

Phylogenomic databases

eggNOGiENOG410IWAX Eukaryota
ENOG410Y2TD LUCA
GeneTreeiENSGT00510000046930
HOGENOMiHOG000015287
HOVERGENiHBG005278
InParanoidiP11942
KOiK06452
OMAiQNCIELS
OrthoDBiEOG091G0MXQ
PhylomeDBiP11942
TreeFamiTF335892

Enzyme and pathway databases

ReactomeiR-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-202424 Downstream TCR signaling
R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains
R-MMU-202430 Translocation of ZAP-70 to Immunological synapse
R-MMU-202433 Generation of second messenger molecules
R-MMU-2029481 FCGR activation
R-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-2029485 Role of phospholipids in phagocytosis
R-MMU-389948 PD-1 signaling
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

EvolutionaryTraceiP11942
PROiPR:P11942
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002033 Expressed in 102 organ(s), highest expression level in thymus
CleanExiMM_CD3G
ExpressionAtlasiP11942 baseline and differential
GenevisibleiP11942 MM

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015484 CD3_esu/gsu/dsu
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR032052 Ig_4
IPR003598 Ig_sub2
IPR003110 Phos_immunorcpt_sig_ITAM
PANTHERiPTHR10570 PTHR10570, 1 hit
PfamiView protein in Pfam
PF16680 Ig_4, 1 hit
PF02189 ITAM, 1 hit
SMARTiView protein in SMART
SM00408 IGc2, 1 hit
SM00077 ITAM, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS51055 ITAM_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCD3G_MOUSE
AccessioniPrimary (citable) accession number: P11942
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 7, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again