UniProtKB - P11942 (CD3G_MOUSE)
T-cell surface glycoprotein CD3 gamma chain
Cd3g
Functioni
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G (PubMed:25920998).
By similarity2 PublicationsGO - Molecular functioni
- identical protein binding Source: MGI
- transmembrane signaling receptor activity Source: UniProtKB
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- cell surface receptor signaling pathway Source: UniProtKB
- establishment or maintenance of cell polarity Source: UniProtKB
- positive thymic T cell selection Source: GO_Central
- protein transport Source: UniProtKB
- regulation of lymphocyte apoptotic process Source: UniProtKB
Keywordsi
Molecular function | Receptor |
Biological process | Adaptive immunity, Immunity |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-202424, Downstream TCR signaling R-MMU-202427, Phosphorylation of CD3 and TCR zeta chains R-MMU-202430, Translocation of ZAP-70 to Immunological synapse R-MMU-202433, Generation of second messenger molecules R-MMU-2029481, FCGR activation R-MMU-2029482, Regulation of actin dynamics for phagocytic cup formation R-MMU-2029485, Role of phospholipids in phagocytosis R-MMU-389948, PD-1 signaling R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: T-cell surface glycoprotein CD3 gamma chainAlternative name(s): T-cell receptor T3 gamma chain CD_antigen: CD3g |
Gene namesi | Name:Cd3g |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88333, Cd3g |
VEuPathDBi | HostDB:ENSMUSG00000002033 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: MGI
Plasma Membrane
- alpha-beta T cell receptor complex Source: MGI
- external side of plasma membrane Source: GO_Central
- plasma membrane Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 23 – 116 | ExtracellularSequence analysisAdd BLAST | 94 | |
Transmembranei | 117 – 137 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 138 – 182 | CytoplasmicSequence analysisAdd BLAST | 45 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Add BLAST | 22 | |
ChainiPRO_0000014617 | 23 – 182 | T-cell surface glycoprotein CD3 gamma chainAdd BLAST | 160 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 46 ↔ 87 | By similarity | ||
Glycosylationi | 66 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 145 | PhosphoserineBy similarity | 1 | |
Modified residuei | 148 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
EPDi | P11942 |
jPOSTi | P11942 |
PaxDbi | P11942 |
PRIDEi | P11942 |
ProteomicsDBi | 281268 |
PTM databases
GlyGeni | P11942, 1 site |
iPTMneti | P11942 |
PhosphoSitePlusi | P11942 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000002033, Expressed in thymus and 129 other tissues |
ExpressionAtlasi | P11942, baseline and differential |
Genevisiblei | P11942, MM |
Interactioni
Subunit structurei
The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta.
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
CORUMi | P11942 |
ELMi | P11942 |
IntActi | P11942, 1 interactor |
STRINGi | 10090.ENSMUSP00000002101 |
Miscellaneous databases
RNActi | P11942, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P11942 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P11942 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 37 – 94 | Ig-likeAdd BLAST | 58 | |
Domaini | 149 – 177 | ITAMPROSITE-ProRule annotationAdd BLAST | 29 |
Keywords - Domaini
Immunoglobulin domain, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S4XC, Eukaryota |
GeneTreei | ENSGT00940000153312 |
HOGENOMi | CLU_115449_0_0_1 |
InParanoidi | P11942 |
OMAi | DRENDQY |
OrthoDBi | 1466902at2759 |
PhylomeDBi | P11942 |
TreeFami | TF335892 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR015484, CD3_esu/gsu/dsu IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR032052, Ig_4 IPR003598, Ig_sub2 IPR003110, Phos_immunorcpt_sig_ITAM |
PANTHERi | PTHR10570, PTHR10570, 1 hit |
Pfami | View protein in Pfam PF16680, Ig_4, 1 hit PF02189, ITAM, 1 hit |
SMARTi | View protein in SMART SM00408, IGc2, 1 hit SM00077, ITAM, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS51055, ITAM_1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MEQRKGLAGL FLVISLLQGT VAQTNKAKNL VQVDGSRGDG SVLLTCGLTD
60 70 80 90 100
KTIKWLKDGS IISPLNATKN TWNLGNNAKD PRGTYQCQGA KETSNPLQVY
110 120 130 140 150
YRMCENCIEL NIGTISGFIF AEVISIFFLA LGVYLIAGQD GVRQSRASDK
160 170 180
QTLLQNEQLY QPLKDREYDQ YSHLQGNQLR KK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE0CXP3 | E0CXP3_MOUSE | T-cell surface glycoprotein CD3 gam... | Cd3g | 100 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00635 mRNA Translation: CAA68667.1 M58149 mRNA Translation: AAA63289.1 Sequence problems. M18227 , M18222, M18223, M18224, M18225, M18226 Genomic DNA Translation: AAA37400.1 BC027528 mRNA Translation: AAH27528.1 |
CCDSi | CCDS23123.1 |
PIRi | A39952, A28508 |
RefSeqi | NP_033980.1, NM_009850.2 |
Genome annotation databases
Ensembli | ENSMUST00000002101; ENSMUSP00000002101; ENSMUSG00000002033 |
GeneIDi | 12502 |
KEGGi | mmu:12502 |
UCSCi | uc009pex.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00635 mRNA Translation: CAA68667.1 M58149 mRNA Translation: AAA63289.1 Sequence problems. M18227 , M18222, M18223, M18224, M18225, M18226 Genomic DNA Translation: AAA37400.1 BC027528 mRNA Translation: AAH27528.1 |
CCDSi | CCDS23123.1 |
PIRi | A39952, A28508 |
RefSeqi | NP_033980.1, NM_009850.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JBJ | NMR | - | A | 23-103 | [»] | |
SMRi | P11942 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
CORUMi | P11942 |
ELMi | P11942 |
IntActi | P11942, 1 interactor |
STRINGi | 10090.ENSMUSP00000002101 |
PTM databases
GlyGeni | P11942, 1 site |
iPTMneti | P11942 |
PhosphoSitePlusi | P11942 |
Proteomic databases
EPDi | P11942 |
jPOSTi | P11942 |
PaxDbi | P11942 |
PRIDEi | P11942 |
ProteomicsDBi | 281268 |
Protocols and materials databases
ABCDi | P11942, 1 sequenced antibody |
Antibodypediai | 4306, 353 antibodies from 41 providers |
DNASUi | 12502 |
Genome annotation databases
Ensembli | ENSMUST00000002101; ENSMUSP00000002101; ENSMUSG00000002033 |
GeneIDi | 12502 |
KEGGi | mmu:12502 |
UCSCi | uc009pex.2, mouse |
Organism-specific databases
CTDi | 917 |
MGIi | MGI:88333, Cd3g |
VEuPathDBi | HostDB:ENSMUSG00000002033 |
Phylogenomic databases
eggNOGi | ENOG502S4XC, Eukaryota |
GeneTreei | ENSGT00940000153312 |
HOGENOMi | CLU_115449_0_0_1 |
InParanoidi | P11942 |
OMAi | DRENDQY |
OrthoDBi | 1466902at2759 |
PhylomeDBi | P11942 |
TreeFami | TF335892 |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-202424, Downstream TCR signaling R-MMU-202427, Phosphorylation of CD3 and TCR zeta chains R-MMU-202430, Translocation of ZAP-70 to Immunological synapse R-MMU-202433, Generation of second messenger molecules R-MMU-2029481, FCGR activation R-MMU-2029482, Regulation of actin dynamics for phagocytic cup formation R-MMU-2029485, Role of phospholipids in phagocytosis R-MMU-389948, PD-1 signaling R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
BioGRID-ORCSi | 12502, 1 hit in 64 CRISPR screens |
ChiTaRSi | Cd3g, mouse |
EvolutionaryTracei | P11942 |
PROi | PR:P11942 |
RNActi | P11942, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000002033, Expressed in thymus and 129 other tissues |
ExpressionAtlasi | P11942, baseline and differential |
Genevisiblei | P11942, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR015484, CD3_esu/gsu/dsu IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR032052, Ig_4 IPR003598, Ig_sub2 IPR003110, Phos_immunorcpt_sig_ITAM |
PANTHERi | PTHR10570, PTHR10570, 1 hit |
Pfami | View protein in Pfam PF16680, Ig_4, 1 hit PF02189, ITAM, 1 hit |
SMARTi | View protein in SMART SM00408, IGc2, 1 hit SM00077, ITAM, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS51055, ITAM_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CD3G_MOUSE | |
Accessioni | P11942Primary (citable) accession number: P11942 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | October 1, 1989 | |
Last modified: | February 23, 2022 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references