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Entry version 111 (02 Dec 2020)
Sequence version 1 (01 Oct 1989)
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Protein

Valine--tRNA ligase

Gene

valS

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=59 µM for valine1 Publication
  2. KM=16 mM for threonine1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei528ATPBy similarity1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionAminoacyl-tRNA synthetase, Ligase
    Biological processProtein biosynthesis
    LigandATP-binding, Nucleotide-binding

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Valine--tRNA ligase (EC:6.1.1.9)
    Alternative name(s):
    Valyl-tRNA synthetase
    Short name:
    ValRS
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:valS
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri1422 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi47P → A: Has little effect on amino acid activation. 1 Publication1
    Mutagenesisi47P → G: Strong decrease in amino acid activation. 1 Publication1
    Mutagenesisi47P → I: Strong decrease in amino acid activation. No effect on posttransfer editing activity. 1 Publication1
    Mutagenesisi85G → R: Strong decrease in amino acid activation. 1 Publication1
    Mutagenesisi87D → A: Strong decrease in amino acid activation. No effect on posttransfer editing activity. 1 Publication1

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001062131 – 880Valine--tRNA ligaseAdd BLAST880

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Monomer.

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P11931

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Coiled coil

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili809 – 880Sequence analysisAdd BLAST72

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi49 – 59'HIGH' regionAdd BLAST11
    Motifi525 – 529'KMSKS' region5

    <p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

    ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.
    The C-terminal coiled-coil domain is crucial for aminoacylation activity.By similarity

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd07962, Anticodon_Ia_Val, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    1.10.287.380, 1 hit
    3.40.50.620, 1 hit
    3.90.740.10, 1 hit

    HAMAP database of protein families

    More...
    HAMAPi
    MF_02004, Val_tRNA_synth_type1, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR001412, aa-tRNA-synth_I_CS
    IPR002300, aa-tRNA-synth_Ia
    IPR033705, Anticodon_Ia_Val
    IPR013155, M/V/L/I-tRNA-synth_anticd-bd
    IPR014729, Rossmann-like_a/b/a_fold
    IPR010978, tRNA-bd_arm
    IPR009080, tRNAsynth_Ia_anticodon-bd
    IPR037118, Val-tRNA_synth_C_sf
    IPR019499, Val-tRNA_synth_tRNA-bd
    IPR009008, Val/Leu/Ile-tRNA-synth_edit
    IPR002303, Valyl-tRNA_ligase

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF08264, Anticodon_1, 1 hit
    PF00133, tRNA-synt_1, 1 hit
    PF10458, Val_tRNA-synt_C, 1 hit

    Protein Motif fingerprint database; a protein domain database

    More...
    PRINTSi
    PR00986, TRNASYNTHVAL

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF46589, SSF46589, 1 hit
    SSF47323, SSF47323, 1 hit
    SSF50677, SSF50677, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR00422, valS, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS00178, AA_TRNA_LIGASE_I, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P11931-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MAQHEVSMPP KYDHRAVEAG RYEWWLKGKF FEATGDPNKR PFTIVIPPPN
    60 70 80 90 100
    VTGKLHLGHA WDTTLQDIIT RMKRMQGYDV LWLPGMDHAG IATQAKVEEK
    110 120 130 140 150
    LRQQGLSRYD LGREKFLEET WKWKEEYAGH IRSQWAKLGL GLDYTRERFT
    160 170 180 190 200
    LDEGLSKAVR EVFVSLYRKG LIYRGEYIIN WDPVTKTALS DIEVVYKEVK
    210 220 230 240 250
    GALYHMRYPL ADGSGFIEVA TTRPETMLGD TAVAVHPDDE RYKHLIGKMV
    260 270 280 290 300
    KLPIVGREIP IIADEYVDME FGSGAVKITP AHDPNDFEIG NRHNLPRILV
    310 320 330 340 350
    MNEDGTMNEN AMQYQGLDRF ECRKQIVRDL QEQGVLFKIE EHVHSVGHSE
    360 370 380 390 400
    RSGAVIEPYL STQWFVKMKP LAEAAIKLQQ TDGKVQFVPE RFEKTYLHWL
    410 420 430 440 450
    ENIRHWCISR QLWWGHRIPA WYHKETGEIY VDHEPPKDIE NWEQDPDVLD
    460 470 480 490 500
    TWFSSALWPF STMGWPDTDS PDYKRYYPTD VLVTGYDIIF FWVSRMIFQG
    510 520 530 540 550
    LEFTGKRPFK DVLIHGLVRD AQGRKMSKSL GNGVDPMDVI DQYGADALRY
    560 570 580 590 600
    FLATGSSPGQ DLRFSTEKVE ATWNFANKIW NASRFALMNM GGMTYEELDL
    610 620 630 640 650
    SGEKTVADHW ILTRLNETIE TVTKLAEKYE FGERGRTLYN FIWDDLCDWY
    660 670 680 690 700
    IEMAKLPLYG DDEAAKKTTR SVLAYVLDNT MRLLHPFMPF ITEEIWQNLP
    710 720 730 740 750
    HEGESITVAP WPQVRPELSN EEAAEEMRML VDIIRAVRNV RAEVNTPPSK
    760 770 780 790 800
    PIALYIKTKD EHVRAALLKN RAYLERFCNP SELLIDTNVP APDKAMTAVV
    810 820 830 840 850
    TGAELIMPLE GLINIEEEIK RLEKELDKWN KEVERVEKKL ANEGFLAKAP
    860 870 880
    AHVVEEERRK RQDYIEKREA VKARLAELKR
    Length:880
    Mass (Da):102,048
    Last modified:October 1, 1989 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i462B0AE4E38B87A2
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    M16318 Genomic DNA Translation: AAA22879.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    A26738, SYBSVS

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M16318 Genomic DNA Translation: AAA22879.1
    PIRiA26738, SYBSVS

    3D structure databases

    SMRiP11931
    ModBaseiSearch...

    Family and domain databases

    CDDicd07962, Anticodon_Ia_Val, 1 hit
    Gene3Di1.10.287.380, 1 hit
    3.40.50.620, 1 hit
    3.90.740.10, 1 hit
    HAMAPiMF_02004, Val_tRNA_synth_type1, 1 hit
    InterProiView protein in InterPro
    IPR001412, aa-tRNA-synth_I_CS
    IPR002300, aa-tRNA-synth_Ia
    IPR033705, Anticodon_Ia_Val
    IPR013155, M/V/L/I-tRNA-synth_anticd-bd
    IPR014729, Rossmann-like_a/b/a_fold
    IPR010978, tRNA-bd_arm
    IPR009080, tRNAsynth_Ia_anticodon-bd
    IPR037118, Val-tRNA_synth_C_sf
    IPR019499, Val-tRNA_synth_tRNA-bd
    IPR009008, Val/Leu/Ile-tRNA-synth_edit
    IPR002303, Valyl-tRNA_ligase
    PfamiView protein in Pfam
    PF08264, Anticodon_1, 1 hit
    PF00133, tRNA-synt_1, 1 hit
    PF10458, Val_tRNA-synt_C, 1 hit
    PRINTSiPR00986, TRNASYNTHVAL
    SUPFAMiSSF46589, SSF46589, 1 hit
    SSF47323, SSF47323, 1 hit
    SSF50677, SSF50677, 1 hit
    TIGRFAMsiTIGR00422, valS, 1 hit
    PROSITEiView protein in PROSITE
    PS00178, AA_TRNA_LIGASE_I, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSYV_GEOSE
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P11931
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: December 2, 2020
    This is version 111 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families
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