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Protein

2'-5'-oligoadenylate synthase 1A

Gene

Oas1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L.1 Publication

Catalytic activityi

3 ATP = pppA2'p5'A2'p5'A + 2 diphosphate.1 Publication3 Publications

Cofactori

Mg2+By similarity

Activity regulationi

Produced as a latent enzyme which is activated by dsRNA generated during the course of viral infection. The dsRNA activator must be at least 15 nucleotides long, and no modification of the 2'-hydroxyl group is tolerated. ssRNA or dsDNA do not act as activators.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64ATPBy similarity1
Metal bindingi76Magnesium; catalyticBy similarity1
Metal bindingi78Magnesium; catalyticBy similarity1
Metal bindingi149Magnesium; catalyticBy similarity1
Binding sitei211ATPBy similarity1
Binding sitei214ATPBy similarity1
Binding sitei231ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
Biological processAntiviral defense, Immunity, Innate immunity
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
2'-5'-oligoadenylate synthase 1A (EC:2.7.7.841 Publication3 Publications)
Short name:
(2-5')oligo(A) synthase 1A
Short name:
2-5A synthase 1A
Alternative name(s):
p42 OAS
Gene namesi
Name:Oas1a
Synonyms:Oias1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2180860 Oas1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Microsome, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001602601 – 3672'-5'-oligoadenylate synthase 1AAdd BLAST367

Proteomic databases

MaxQBiP11928
PaxDbiP11928
PeptideAtlasiP11928
PRIDEiP11928

PTM databases

PhosphoSitePlusiP11928
SwissPalmiP11928

Expressioni

Tissue specificityi

Expressed in oocytes and granulosa cells of ovary, in intestine, stomach, spleen and uterus (at protein level) (PubMed:15899864). Expressed at high levels in the digestive tract and lymphoid organs (PubMed:12396720). Expressed in ovary and spleen (PubMed:27663720).3 Publications

Developmental stagei

Detected 5 weeks after birth in developing ovary and spleen (PubMed:27663720). Expressed in fully grown, germinal vesicle (GV)-intact oocytes, in oocytes at the metaphase II stage and in the 2- to 8-cell embryos (PubMed:15899864).2 Publications

Inductioni

Up-regulated in response to the type I interferon IFNB1, in a STAT1 and STAT2-dependent manner (PubMed:1709495, PubMed:22305621). Induced by polyinosinic:polycytidylic acid (poly I:C) (PubMed:15899864, PubMed:27663720).4 Publications

Gene expression databases

BgeeiENSMUSG00000052776 Expressed in 20 organ(s), highest expression level in bone marrow macrophage
GenevisibleiP11928 MM

Interactioni

Subunit structurei

Monomer (By similarity). Homotetramer (By similarity). Interacts with OAS1D; the interaction inhibits OAS1A catalytic activity (PubMed:15899864).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
O891685EBI-9352099,EBI-10107989From Hepacivirus C.

Protein-protein interaction databases

BioGridi232938, 1 interactor
IntActiP11928, 1 interactor
STRINGi10090.ENSMUSP00000079198

Structurei

3D structure databases

ProteinModelPortaliP11928
SMRiP11928
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 61Interaction with dsRNABy similarityAdd BLAST48
Regioni201 – 211Interaction with dsRNABy similarityAdd BLAST11

Sequence similaritiesi

Belongs to the 2-5A synthase family.Curated

Phylogenomic databases

eggNOGiKOG0001 Eukaryota
COG5272 LUCA
GeneTreeiENSGT00510000046406
HOGENOMiHOG000022614
HOVERGENiHBG000994
InParanoidiP11928
KOiK14216
OMAiENWTCTI
OrthoDBiEOG091G0160
TreeFamiTF329749

Family and domain databases

Gene3Di1.10.1410.20, 1 hit
InterProiView protein in InterPro
IPR006117 2-5-oligoadenylate_synth_CS
IPR006116 2-5-oligoadenylate_synth_N
IPR018952 2-5-oligoAdlate_synth_1_dom2/C
IPR038121 2-5-oligoAdlate_synth_2_sf
IPR026774 2-5A_synthase
IPR002934 Polymerase_NTP_transf_dom
PANTHERiPTHR11258 PTHR11258, 1 hit
PfamiView protein in Pfam
PF01909 NTP_transf_2, 1 hit
PF10421 OAS1_C, 1 hit
PROSITEiView protein in PROSITE
PS00832 25A_SYNTH_1, 1 hit
PS00833 25A_SYNTH_2, 1 hit
PS50152 25A_SYNTH_3, 1 hit

Sequencei

Sequence statusi: Complete.

P11928-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEHGLRSIPA WTLDKFIEDY LLPDTTFGAD VKSAVNVVCD FLKERCFQGA
60 70 80 90 100
AHPVRVSKVV KGGSSGKGTT LKGKSDADLV VFLNNLTSFE DQLNRRGEFI
110 120 130 140 150
KEIKKQLYEV QHERRFRVKF EVQSSWWPNA RSLSFKLSAP HLHQEVEFDV
160 170 180 190 200
LPAFDVLGHV NTSSKPDPRI YAILIEECTS LGKDGEFSTC FTELQRNFLK
210 220 230 240 250
QRPTKLKSLI RLVKHWYQLC KEKLGKPLPP QYALELLTVF AWEQGNGCYE
260 270 280 290 300
FNTAQGFRTV LELVINYQHL RIYWTKYYDF QHQEVSKYLH RQLRKARPVI
310 320 330 340 350
LDPADPTGNV AGGNPEGWRR LAEEADVWLW YPCFIKKDGS RVSSWDVPTV
360
VPVPFEQVEE NWTCILL
Length:367
Mass (Da):42,429
Last modified:July 27, 2011 - v2
Checksum:iAE5946D5807776A4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74K → R in CAA28620 (PubMed:3808949).Curated1
Sequence conflicti74K → R in AAA37116 (PubMed:2171206).Curated1
Sequence conflicti176E → W in CAA41105 (PubMed:1709495).Curated1
Sequence conflicti240F → Y in CAA41105 (PubMed:1709495).Curated1
Sequence conflicti249Y → N in CAA41105 (PubMed:1709495).Curated1
Sequence conflicti283Q → K in CAA41105 (PubMed:1709495).Curated1
Sequence conflicti329L → V in AAH57878 (PubMed:15489334).Curated1
Sequence conflicti329L → V in AAH13715 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04958 mRNA Translation: CAA28620.1
M33863 mRNA Translation: AAA37116.1
AK149613 mRNA Translation: BAE28990.1
AC015535 Genomic DNA No translation available.
CH466529 Genomic DNA Translation: EDL19743.1
BC013715 mRNA Translation: AAH13715.1
BC057878 mRNA Translation: AAH57878.1
X58077 mRNA Translation: CAA41105.1
CCDSiCCDS19630.1
PIRiA24725 SYMSO1
S15660
RefSeqiNP_660212.2, NM_145211.2
UniGeneiMm.14301
Mm.389688

Genome annotation databases

EnsembliENSMUST00000080322; ENSMUSP00000079198; ENSMUSG00000052776
GeneIDi246730
KEGGimmu:246730
UCSCiuc008zij.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04958 mRNA Translation: CAA28620.1
M33863 mRNA Translation: AAA37116.1
AK149613 mRNA Translation: BAE28990.1
AC015535 Genomic DNA No translation available.
CH466529 Genomic DNA Translation: EDL19743.1
BC013715 mRNA Translation: AAH13715.1
BC057878 mRNA Translation: AAH57878.1
X58077 mRNA Translation: CAA41105.1
CCDSiCCDS19630.1
PIRiA24725 SYMSO1
S15660
RefSeqiNP_660212.2, NM_145211.2
UniGeneiMm.14301
Mm.389688

3D structure databases

ProteinModelPortaliP11928
SMRiP11928
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232938, 1 interactor
IntActiP11928, 1 interactor
STRINGi10090.ENSMUSP00000079198

PTM databases

PhosphoSitePlusiP11928
SwissPalmiP11928

Proteomic databases

MaxQBiP11928
PaxDbiP11928
PeptideAtlasiP11928
PRIDEiP11928

Protocols and materials databases

DNASUi246730
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080322; ENSMUSP00000079198; ENSMUSG00000052776
GeneIDi246730
KEGGimmu:246730
UCSCiuc008zij.2 mouse

Organism-specific databases

CTDi246730
MGIiMGI:2180860 Oas1a

Phylogenomic databases

eggNOGiKOG0001 Eukaryota
COG5272 LUCA
GeneTreeiENSGT00510000046406
HOGENOMiHOG000022614
HOVERGENiHBG000994
InParanoidiP11928
KOiK14216
OMAiENWTCTI
OrthoDBiEOG091G0160
TreeFamiTF329749

Miscellaneous databases

PROiPR:P11928
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052776 Expressed in 20 organ(s), highest expression level in bone marrow macrophage
GenevisibleiP11928 MM

Family and domain databases

Gene3Di1.10.1410.20, 1 hit
InterProiView protein in InterPro
IPR006117 2-5-oligoadenylate_synth_CS
IPR006116 2-5-oligoadenylate_synth_N
IPR018952 2-5-oligoAdlate_synth_1_dom2/C
IPR038121 2-5-oligoAdlate_synth_2_sf
IPR026774 2-5A_synthase
IPR002934 Polymerase_NTP_transf_dom
PANTHERiPTHR11258 PTHR11258, 1 hit
PfamiView protein in Pfam
PF01909 NTP_transf_2, 1 hit
PF10421 OAS1_C, 1 hit
PROSITEiView protein in PROSITE
PS00832 25A_SYNTH_1, 1 hit
PS00833 25A_SYNTH_2, 1 hit
PS50152 25A_SYNTH_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOAS1A_MOUSE
AccessioniPrimary (citable) accession number: P11928
Secondary accession number(s): F2Z419
, Q3UEC1, Q64440, Q91VI0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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