UniProtKB - P11880 (MURF_ECOLI)
Protein
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
Gene
murF
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.UniRule annotation1 Publication
Catalytic activityi
- ATP + D-alanyl-D-alanine + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + H+ + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanineUniRule annotation1 PublicationEC:6.3.2.10UniRule annotation1 Publication
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 107 – 113 | ATPUniRule annotation | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Source: EcoCyc
- UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Source: EcoCyc
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: EcoCyc
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:UDP-NACMURALGLDAPAALIG-MONOMER MetaCyc:UDP-NACMURALGLDAPAALIG-MONOMER |
BRENDAi | 6.3.2.10, 2026 6.3.2.8, 2026 |
SABIO-RKi | P11880 |
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotationCurated (EC:6.3.2.10UniRule annotation1 Publication)Alternative name(s): D-alanyl-D-alanine-adding enzyme1 PublicationUniRule annotation UDP-MurNAc-pentapeptide synthetase1 Publication |
Gene namesi | Name:murF1 PublicationUniRule annotation Synonyms:mra Ordered Locus Names:b0086, JW0084 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101698 | 1 – 452 | UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseAdd BLAST | 452 |
Proteomic databases
jPOSTi | P11880 |
PaxDbi | P11880 |
PRIDEi | P11880 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4261642, 419 interactors 849214, 1 interactor |
DIPi | DIP-10281N |
IntActi | P11880, 11 interactors |
STRINGi | 511145.b0086 |
Chemistry databases
BindingDBi | P11880 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P11880 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P11880 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0770, Bacteria |
HOGENOMi | CLU_031507_4_0_6 |
InParanoidi | P11880 |
PhylomeDBi | P11880 |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_02019, MurF, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N IPR035911, MurE/MurF_N IPR005863, UDP-N-AcMur_synth |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit SSF63418, SSF63418, 1 hit |
TIGRFAMsi | TIGR01143, murF, 1 hit |
i Sequence
Sequence statusi: Complete.
P11880-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MISVTLSQLT DILNGELQGA DITLDAVTTD TRKLTPGCLF VALKGERFDA
60 70 80 90 100
HDFADQAKAG GAGALLVSRP LDIDLPQLIV KDTRLAFGEL AAWVRQQVPA
110 120 130 140 150
RVVALTGSSG KTSVKEMTAA ILSQCGNTLY TAGNLNNDIG VPMTLLRLTP
160 170 180 190 200
EYDYAVIELG ANHQGEIAWT VSLTRPEAAL VNNLAAAHLE GFGSLAGVAK
210 220 230 240 250
AKGEIFSGLP ENGIAIMNAD NNDWLNWQSV IGSRKVWRFS PNAANSDFTA
260 270 280 290 300
TNIHVTSHGT EFTLQTPTGS VDVLLPLPGR HNIANALAAA ALSMSVGATL
310 320 330 340 350
DAIKAGLANL KAVPGRLFPI QLAENQLLLD DSYNANVGSM TAAVQVLAEM
360 370 380 390 400
PGYRVLVVGD MAELGAESEA CHVQVGEAAK AAGIDRVLSV GKQSHAISTA
410 420 430 440 450
SGVGEHFADK TALITRLKLL IAEQQVITIL VKGSRSAAME EVVRALQENG
TC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 61 | G → A (PubMed:2668880).Curated | 1 | |
Sequence conflicti | 61 | G → A (PubMed:1630901).Curated | 1 | |
Sequence conflicti | 178 | A → R (PubMed:2668880).Curated | 1 | |
Sequence conflicti | 178 | A → R (PubMed:1630901).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 288 | A → T in murF2; temperature-sensitive mutant with low activity. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15432 Genomic DNA Translation: CAA33473.1 X55034 Genomic DNA Translation: CAA38863.1 U67891 Genomic DNA Translation: AAC44657.1 U00096 Genomic DNA Translation: AAC73197.1 AP009048 Genomic DNA Translation: BAB96654.2 U67893 Genomic DNA Translation: AAB60788.1 |
PIRi | F64730 |
RefSeqi | NP_414628.1, NC_000913.3 WP_000626685.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73197; AAC73197; b0086 BAB96654; BAB96654; BAB96654 |
GeneIDi | 944813 |
KEGGi | ecj:JW0084 eco:b0086 |
PATRICi | fig|1411691.4.peg.2194 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15432 Genomic DNA Translation: CAA33473.1 X55034 Genomic DNA Translation: CAA38863.1 U67891 Genomic DNA Translation: AAC44657.1 U00096 Genomic DNA Translation: AAC73197.1 AP009048 Genomic DNA Translation: BAB96654.2 U67893 Genomic DNA Translation: AAB60788.1 |
PIRi | F64730 |
RefSeqi | NP_414628.1, NC_000913.3 WP_000626685.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GG4 | X-ray | 2.30 | A/B | 1-452 | [»] | |
SMRi | P11880 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261642, 419 interactors 849214, 1 interactor |
DIPi | DIP-10281N |
IntActi | P11880, 11 interactors |
STRINGi | 511145.b0086 |
Chemistry databases
BindingDBi | P11880 |
ChEMBLi | CHEMBL2540 |
Proteomic databases
jPOSTi | P11880 |
PaxDbi | P11880 |
PRIDEi | P11880 |
Genome annotation databases
EnsemblBacteriai | AAC73197; AAC73197; b0086 BAB96654; BAB96654; BAB96654 |
GeneIDi | 944813 |
KEGGi | ecj:JW0084 eco:b0086 |
PATRICi | fig|1411691.4.peg.2194 |
Organism-specific databases
EchoBASEi | EB0617 |
Phylogenomic databases
eggNOGi | COG0770, Bacteria |
HOGENOMi | CLU_031507_4_0_6 |
InParanoidi | P11880 |
PhylomeDBi | P11880 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:UDP-NACMURALGLDAPAALIG-MONOMER MetaCyc:UDP-NACMURALGLDAPAALIG-MONOMER |
BRENDAi | 6.3.2.10, 2026 6.3.2.8, 2026 |
SABIO-RKi | P11880 |
Miscellaneous databases
EvolutionaryTracei | P11880 |
PROi | PR:P11880 |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_02019, MurF, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR000713, Mur_ligase_N IPR035911, MurE/MurF_N IPR005863, UDP-N-AcMur_synth |
Pfami | View protein in Pfam PF01225, Mur_ligase, 1 hit PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit SSF63418, SSF63418, 1 hit |
TIGRFAMsi | TIGR01143, murF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MURF_ECOLI | |
Accessioni | P11880Primary (citable) accession number: P11880 Secondary accession number(s): O07100, P77636 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 191 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families