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UniProtKB - P11880 (MURF_ECOLI)

Entry version 194 (25 May 2022)
Sequence version 2 (01 Nov 1997)
Previous versions | rss
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Protein

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase

Gene

murF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.

UniRule annotation1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi107 – 113ATPUniRule annotation7

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:UDP-NACMURALGLDAPAALIG-MONOMER

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		6.3.2.10, 2026
6.3.2.8, 2026

SABIO-RK: Biochemical Reaction Kinetics Database

More...SABIO-RKi		P11880

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00219

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotationCurated (EC:6.3.2.10UniRule annotation1 Publication)
Alternative name(s):
D-alanyl-D-alanine-adding enzyme1 PublicationUniRule annotation
UDP-MurNAc-pentapeptide synthetase1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:murF1 PublicationUniRule annotation
Synonyms:mra
Ordered Locus Names:b0086, JW0084
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL2540

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001016981 – 452UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseAdd BLAST452

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P11880

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P11880

PRoteomics IDEntifications database

More...PRIDEi		P11880

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer.

1 Publication

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4261642, 419 interactors
849214, 1 interactor

Database of interacting proteins

More...DIPi		DIP-10281N

Protein interaction database and analysis system

More...IntActi		P11880, 11 interactors

STRING: functional protein association networks

More...STRINGi		511145.b0086

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		P11880

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1452
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		P11880

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P11880

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P11880

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the MurCDEF family. MurF subfamily.UniRule annotationCurated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0770, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_031507_4_0_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P11880

Identification of Orthologs from Complete Genome Data

More...OMAi		VEMGANH

Database for complete collections of gene phylogenies

More...PhylomeDBi		P11880

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.40.1190.10, 1 hit
3.90.190.20, 1 hit

HAMAP database of protein families

More...HAMAPi		MF_02019, MurF, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR036565, Mur-like_cat_sf
IPR004101, Mur_ligase_C
IPR036615, Mur_ligase_C_dom_sf
IPR013221, Mur_ligase_cen
IPR000713, Mur_ligase_N
IPR035911, MurE/MurF_N
IPR005863, UDP-N-AcMur_synth

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01225, Mur_ligase, 1 hit
PF02875, Mur_ligase_C, 1 hit
PF08245, Mur_ligase_M, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF53244, SSF53244, 1 hit
SSF53623, SSF53623, 1 hit
SSF63418, SSF63418, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR01143, murF, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P11880-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MISVTLSQLT DILNGELQGA DITLDAVTTD TRKLTPGCLF VALKGERFDA 
        60         70         80         90        100
HDFADQAKAG GAGALLVSRP LDIDLPQLIV KDTRLAFGEL AAWVRQQVPA 
       110        120        130        140        150
RVVALTGSSG KTSVKEMTAA ILSQCGNTLY TAGNLNNDIG VPMTLLRLTP 
       160        170        180        190        200
EYDYAVIELG ANHQGEIAWT VSLTRPEAAL VNNLAAAHLE GFGSLAGVAK 
       210        220        230        240        250
AKGEIFSGLP ENGIAIMNAD NNDWLNWQSV IGSRKVWRFS PNAANSDFTA 
       260        270        280        290        300
TNIHVTSHGT EFTLQTPTGS VDVLLPLPGR HNIANALAAA ALSMSVGATL 
       310        320        330        340        350
DAIKAGLANL KAVPGRLFPI QLAENQLLLD DSYNANVGSM TAAVQVLAEM 
       360        370        380        390        400
PGYRVLVVGD MAELGAESEA CHVQVGEAAK AAGIDRVLSV GKQSHAISTA 
       410        420        430        440        450
SGVGEHFADK TALITRLKLL IAEQQVITIL VKGSRSAAME EVVRALQENG 

TC
Length:452
Mass (Da):47,447
Last modified:November 1, 1997 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB46E2E57BDBBC572
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti61G → A (PubMed:2668880).Curated1
Sequence conflicti61G → A (PubMed:1630901).Curated1
Sequence conflicti178A → R (PubMed:2668880).Curated1
Sequence conflicti178A → R (PubMed:1630901).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural varianti288A → T in murF2; temperature-sensitive mutant with low activity. 1 Publication1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X15432 Genomic DNA Translation: CAA33473.1
X55034 Genomic DNA Translation: CAA38863.1
U67891 Genomic DNA Translation: AAC44657.1
U00096 Genomic DNA Translation: AAC73197.1
AP009048 Genomic DNA Translation: BAB96654.2
U67893 Genomic DNA Translation: AAB60788.1

Protein sequence database of the Protein Information Resource

More...PIRi		F64730

NCBI Reference Sequences

More...RefSeqi		NP_414628.1, NC_000913.3
WP_000626685.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC73197; AAC73197; b0086
BAB96654; BAB96654; BAB96654

Database of genes from NCBI RefSeq genomes

More...GeneIDi		944813

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW0084
eco:b0086

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.2194

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15432 Genomic DNA Translation: CAA33473.1
X55034 Genomic DNA Translation: CAA38863.1
U67891 Genomic DNA Translation: AAC44657.1
U00096 Genomic DNA Translation: AAC73197.1
AP009048 Genomic DNA Translation: BAB96654.2
U67893 Genomic DNA Translation: AAB60788.1
PIRiF64730
RefSeqiNP_414628.1, NC_000913.3
WP_000626685.1, NZ_LN832404.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GG4X-ray2.30A/B1-452[»]
AlphaFoldDBiP11880
SMRiP11880
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi4261642, 419 interactors
849214, 1 interactor
DIPiDIP-10281N
IntActiP11880, 11 interactors
STRINGi511145.b0086

Chemistry databases

BindingDBiP11880
ChEMBLiCHEMBL2540

Proteomic databases

jPOSTiP11880
PaxDbiP11880
PRIDEiP11880

Genome annotation databases

EnsemblBacteriaiAAC73197; AAC73197; b0086
BAB96654; BAB96654; BAB96654
GeneIDi944813
KEGGiecj:JW0084
eco:b0086
PATRICifig|1411691.4.peg.2194

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB0617

Phylogenomic databases

eggNOGiCOG0770, Bacteria
HOGENOMiCLU_031507_4_0_6
InParanoidiP11880
OMAiVEMGANH
PhylomeDBiP11880

Enzyme and pathway databases

UniPathwayiUPA00219
BioCyciEcoCyc:UDP-NACMURALGLDAPAALIG-MONOMER
BRENDAi6.3.2.10, 2026
6.3.2.8, 2026
SABIO-RKiP11880

Miscellaneous databases

EvolutionaryTraceiP11880

Protein Ontology

More...PROi		PR:P11880

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_02019, MurF, 1 hit
InterProiView protein in InterPro
IPR036565, Mur-like_cat_sf
IPR004101, Mur_ligase_C
IPR036615, Mur_ligase_C_dom_sf
IPR013221, Mur_ligase_cen
IPR000713, Mur_ligase_N
IPR035911, MurE/MurF_N
IPR005863, UDP-N-AcMur_synth
PfamiView protein in Pfam
PF01225, Mur_ligase, 1 hit
PF02875, Mur_ligase_C, 1 hit
PF08245, Mur_ligase_M, 1 hit
SUPFAMiSSF53244, SSF53244, 1 hit
SSF53623, SSF53623, 1 hit
SSF63418, SSF63418, 1 hit
TIGRFAMsiTIGR01143, murF, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMURF_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P11880
Secondary accession number(s): O07100, P77636
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: November 1, 1997
Last modified: May 25, 2022
This is version 194 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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