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Protein

Serum response factor

Gene

SRF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi133 – 222Add BLAST90

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-5663220 RHO GTPases Activate Formins
SignaLinkiP11831
SIGNORiP11831

Names & Taxonomyi

Protein namesi
Recommended name:
Serum response factor
Short name:
SRF
Gene namesi
Name:SRF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000112658.7
HGNCiHGNC:11291 SRF
MIMi600589 gene
neXtProtiNX_P11831

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6722
OpenTargetsiENSG00000112658
PharmGKBiPA36116

Polymorphism and mutation databases

BioMutaiSRF
DMDMi134876

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001994231 – 508Serum response factorAdd BLAST508

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei77PhosphoserineCombined sources1 Publication1
Modified residuei79PhosphoserineCombined sources1 Publication1
Modified residuei83PhosphoserineCombined sources1 Publication1
Modified residuei85PhosphoserineCombined sources1 Publication1
Modified residuei103Phosphoserine1 Publication1
Modified residuei224PhosphoserineCombined sources1
Modified residuei253Phosphoserine1 Publication1
GlycosylationiCAR_000181277O-linked (GlcNAc) serine1 Publication1
Glycosylationi307O-linked (GlcNAc) serine1 Publication1
Glycosylationi309O-linked (GlcNAc) serine1 Publication1
GlycosylationiCAR_000196316O-linked (GlcNAc) serine1 Publication1
Glycosylationi383O-linked (GlcNAc) serine1 Publication1
Modified residuei435Phosphoserine; by dsDNA kinase1 Publication1
Modified residuei446Phosphoserine; by dsDNA kinase1 Publication1

Post-translational modificationi

Phosphorylated by PRKDC.3 Publications

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP11831
MaxQBiP11831
PaxDbiP11831
PeptideAtlasiP11831
PRIDEiP11831
ProteomicsDBi52806

PTM databases

GlyConnecti561
iPTMnetiP11831
PhosphoSitePlusiP11831
UniCarbKBiP11831

Miscellaneous databases

PMAP-CutDBiP11831

Expressioni

Gene expression databases

BgeeiENSG00000112658 Expressed in 229 organ(s), highest expression level in lower esophagus
CleanExiHS_SRF
ExpressionAtlasiP11831 baseline and differential
GenevisibleiP11831 HS

Organism-specific databases

HPAiCAB005416
HPA001819

Interactioni

Subunit structurei

Binds DNA as a multimer, probably a dimer (PubMed:7637780). Interacts with MRTFA, forming the SRF-MRTFA nuclear complex which binds the 5'-CArG-3' consensus motif (CArG box) on DNA via SRF (PubMed:14565952, PubMed:19350017). Forms a nuclear ternary complex with MRTFA and SCAI (PubMed:19350017). Interacts with MRTFB (PubMed:14565952). Interacts with MLLT7/FOXO4, NKX3A and SSRP1 (PubMed:16054032). Interacts with ARID2 (By similarity). Interacts with SRFBP1 (By similarity). Interacts with LPXN (PubMed:18497331). Interacts with OLFM2; the interaction promotes dissociation of SRF from the transcriptional repressor HEY2, facilitates binding of SRF to target genes and promotes smooth muscle differentiation (PubMed:25298399).By similarity6 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112600, 62 interactors
CORUMiP11831
DIPiDIP-49N
ELMiP11831
IntActiP11831, 8 interactors
MINTiP11831
STRINGi9606.ENSP00000265354

Structurei

Secondary structure

1508
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP11831
SMRiP11831
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11831

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini141 – 201MADS-boxPROSITE-ProRule annotationAdd BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni168 – 222Involved in dimerizationAdd BLAST55

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 142Gly-richAdd BLAST130
Compositional biasi80 – 90Asp/Glu-rich (acidic)Add BLAST11
Compositional biasi242 – 258Asp/Glu-rich (acidic)Add BLAST17

Phylogenomic databases

eggNOGiKOG0015 Eukaryota
COG5068 LUCA
GeneTreeiENSGT00400000022158
HOGENOMiHOG000012380
HOVERGENiHBG014968
InParanoidiP11831
KOiK04378
OMAiTSTSMQV
OrthoDBiEOG091G0X24
PhylomeDBiP11831
TreeFamiTF318482

Family and domain databases

CDDicd00266 MADS_SRF_like, 1 hit
Gene3Di3.40.1810.10, 1 hit
InterProiView protein in InterPro
IPR033897 MADS_SRF-like
IPR002100 TF_MADSbox
IPR036879 TF_MADSbox_sf
PfamiView protein in Pfam
PF00319 SRF-TF, 1 hit
PRINTSiPR00404 MADSDOMAIN
SMARTiView protein in SMART
SM00432 MADS, 1 hit
SUPFAMiSSF55455 SSF55455, 1 hit
PROSITEiView protein in PROSITE
PS00350 MADS_BOX_1, 1 hit
PS50066 MADS_BOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

P11831-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLPTQAGAAA ALGRGSALGG SLNRTPTGRP GGGGGTRGAN GGRVPGNGAG
60 70 80 90 100
LGPGRLEREA AAAAATTPAP TAGALYSGSE GDSESGEEEE LGAERRGLKR
110 120 130 140 150
SLSEMEIGMV VGGPEASAAA TGGYGPVSGA VSGAKPGKKT RGRVKIKMEF
160 170 180 190 200
IDNKLRRYTT FSKRKTGIMK KAYELSTLTG TQVLLLVASE TGHVYTFATR
210 220 230 240 250
KLQPMITSET GKALIQTCLN SPDSPPRSDP TTDQRMSATG FEETDLTYQV
260 270 280 290 300
SESDSSGETK DTLKPAFTVT NLPGTTSTIQ TAPSTSTTMQ VSSGPSFPIT
310 320 330 340 350
NYLAPVSASV SPSAVSSANG TVLKSTGSGP VSSGGLMQLP TSFTLMPGGA
360 370 380 390 400
VAQQVPVQAI QVHQAPQQAS PSRDSSTDLT QTSSSGTVTL PATIMTSSVP
410 420 430 440 450
TTVGGHMMYP SPHAVMYAPT SGLGDGSLTV LNAFSQAPST MQVSHSQVQE
460 470 480 490 500
PGGVPQVFLT ASSGTVQIPV SAVQLHQMAV IGQQAGSSSN LTELQVVNLD

TAHSTKSE
Length:508
Mass (Da):51,593
Last modified:October 1, 1989 - v1
Checksum:i25505828D3276F44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03161 mRNA Translation: AAA36647.1
AL133375 Genomic DNA No translation available.
AL355385 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX04161.1
BC048211 mRNA Translation: AAH48211.1
BC052572 mRNA Translation: AAH52572.1
CCDSiCCDS4889.1
PIRiA31637
RefSeqiNP_001278930.1, NM_001292001.1
NP_003122.1, NM_003131.3
UniGeneiHs.520140

Genome annotation databases

EnsembliENST00000265354; ENSP00000265354; ENSG00000112658
GeneIDi6722
KEGGihsa:6722
UCSCiuc003oui.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03161 mRNA Translation: AAA36647.1
AL133375 Genomic DNA No translation available.
AL355385 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX04161.1
BC048211 mRNA Translation: AAH48211.1
BC052572 mRNA Translation: AAH52572.1
CCDSiCCDS4889.1
PIRiA31637
RefSeqiNP_001278930.1, NM_001292001.1
NP_003122.1, NM_003131.3
UniGeneiHs.520140

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HBXX-ray3.15A/B/D/E132-223[»]
1K6OX-ray3.19B/C133-235[»]
1SRSX-ray3.20A/B132-223[»]
ProteinModelPortaliP11831
SMRiP11831
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112600, 62 interactors
CORUMiP11831
DIPiDIP-49N
ELMiP11831
IntActiP11831, 8 interactors
MINTiP11831
STRINGi9606.ENSP00000265354

PTM databases

GlyConnecti561
iPTMnetiP11831
PhosphoSitePlusiP11831
UniCarbKBiP11831

Polymorphism and mutation databases

BioMutaiSRF
DMDMi134876

Proteomic databases

EPDiP11831
MaxQBiP11831
PaxDbiP11831
PeptideAtlasiP11831
PRIDEiP11831
ProteomicsDBi52806

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265354; ENSP00000265354; ENSG00000112658
GeneIDi6722
KEGGihsa:6722
UCSCiuc003oui.4 human

Organism-specific databases

CTDi6722
DisGeNETi6722
EuPathDBiHostDB:ENSG00000112658.7
GeneCardsiSRF
HGNCiHGNC:11291 SRF
HPAiCAB005416
HPA001819
MIMi600589 gene
neXtProtiNX_P11831
OpenTargetsiENSG00000112658
PharmGKBiPA36116
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0015 Eukaryota
COG5068 LUCA
GeneTreeiENSGT00400000022158
HOGENOMiHOG000012380
HOVERGENiHBG014968
InParanoidiP11831
KOiK04378
OMAiTSTSMQV
OrthoDBiEOG091G0X24
PhylomeDBiP11831
TreeFamiTF318482

Enzyme and pathway databases

ReactomeiR-HSA-5663220 RHO GTPases Activate Formins
SignaLinkiP11831
SIGNORiP11831

Miscellaneous databases

EvolutionaryTraceiP11831
GeneWikiiSerum_response_factor
GenomeRNAii6722
PMAP-CutDBiP11831
PROiPR:P11831
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000112658 Expressed in 229 organ(s), highest expression level in lower esophagus
CleanExiHS_SRF
ExpressionAtlasiP11831 baseline and differential
GenevisibleiP11831 HS

Family and domain databases

CDDicd00266 MADS_SRF_like, 1 hit
Gene3Di3.40.1810.10, 1 hit
InterProiView protein in InterPro
IPR033897 MADS_SRF-like
IPR002100 TF_MADSbox
IPR036879 TF_MADSbox_sf
PfamiView protein in Pfam
PF00319 SRF-TF, 1 hit
PRINTSiPR00404 MADSDOMAIN
SMARTiView protein in SMART
SM00432 MADS, 1 hit
SUPFAMiSSF55455 SSF55455, 1 hit
PROSITEiView protein in PROSITE
PS00350 MADS_BOX_1, 1 hit
PS50066 MADS_BOX_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSRF_HUMAN
AccessioniPrimary (citable) accession number: P11831
Secondary accession number(s): Q5T648
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: October 10, 2018
This is version 207 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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