UniProtKB - P11828 (GLYG3_SOYBN)
Protein
Glycinin G3
Gene
GY3
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Functioni
Glycinin is the major seed storage protein of soybean (PubMed:2485233). Glycinin basic peptides (GBPs), and, to a lower extent, glycinin exhibit antibacterial activity against Gram-negative and Gram-positive bacteria (e.g. L.monocytogenes, B.subtilis, E.coli and S.enteritidis) by forming pores and aggregating in transmembranes, leading to membrane permeability and, eventually, cell death (PubMed:22236762, Ref. 12, PubMed:28590128).4 Publications
GO - Molecular functioni
- nutrient reservoir activity Source: UniProtKB-KW
Keywordsi
Molecular function | Seed storage protein, Storage protein |
Names & Taxonomyi
Protein namesi | Recommended name: Glycinin G32 PublicationsShort name: Glycinin 11S G3Curated Short name: Glycinin A1bB21 Publication Alternative name(s): Allergen: Gly m 6Curated Cleaved into the following 2 chains: Glycinin A1b subunit1 Publication Alternative name(s): Glycinin A subunit Glycinin B2 subunit1 Publication Alternative name(s): Glycinin B subunit |
Gene namesi | Name:GY31 Publication Ordered Locus Names:Glyma19g34780Curated |
Organismi | Glycine max (Soybean) (Glycine hispida) |
Taxonomic identifieri | 3847 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Fabales › Fabaceae › Papilionoideae › 50 kb inversion clade › NPAAA clade › indigoferoid/millettioid clade › Phaseoleae › Glycine › Glycine subgen. Soja |
Proteomesi |
|
Subcellular locationi
Vacuole
- Protein storage vacuole By similarity
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Note: Hexamers are assembled in the endoplasmic reticulum and later sorted to the protein storage vacuoles.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Vacuole
- protein storage vacuole Source: UniProtKB
Keywords - Cellular componenti
Endoplasmic reticulum, VacuolePathology & Biotechi
Allergenic propertiesi
Causes an allergic reaction in human and animals (e.g. rats, mouse and piglets); the acidic subunit is particularly allergenic (PubMed:18996574, PubMed:24499064, PubMed:23426933). Binds to IgE of patients with severe allergic reactions (anaphylaxis) to soybean (PubMed:18996574). Allergy to soybean is most common for infants (usually appears at the age of three months) which frequently outgrow their soybean allergy by the age of two, but a severe soybean allergy can last a lifetime; various symptoms involve skin, gastrointestinal tract and respiratory tracts (PubMed:24499064). Damaged intestinal function in piglets is associated with glycinin-mediated perturbation of nuclear factor-kappa B (NF-kappaB), Jun N-terminal kinase (JNK) and p38 levels (PubMed:30139257). Juvenile Chinese mitten crabs (E.sinensis) supplemented with glycinin display impaired growth and altered intestinal health due to gut inflammation, reshaped community of gut microbiota and digestive dysfunction (PubMed:30300740). Ingredient processing methods to reduce soybean allergenicity but keeping its nutritional values have been developed, among them physical processing includes extrusion, high-pressure (>300 MPa), heating (between 70 and 90 degrees Celsius), roasting, chemical processing includes ethanol extraction (55-76 percent between 70 and 80 degrees Celsius), in vitro glycation (e.g. with xylose at 55 degrees Celsius) and enzymatic hydrolysis with pepsin and trypsin, and biological processing includes fermentation with A.oryzae, S.cerevisiae, L.lactic subsplactis, B.subtilis, B.lactic and L.plantarum (PubMed:24499064, PubMed:27620509). Resistant to hydrolysis by papain, alcalase, and fungal protease (PubMed:24499064).1 Publication5 Publications
Biotechnological usei
Emulsification efficiency of glycinin is improved by degree-dependent glycation with soy soluble polysaccharide (SSPS) at 60 degrees Celsius in both the acidic (A) and basic (B) polypeptides as a result of subunit dissociation at the quaternary level (PubMed:30372068). Thermal treatment of soybean seed proteins leads to the aggregation of glycinin acidic and basic polypeptides (GAP and GBP, respectively) (PubMed:10867183, PubMed:25801436). GBP improve sensory properties of meat (e.g. pork) during chilled storage and inhibit bacterial growth (e.g. L.monocytogenes, B.subtilis, E.coli and S.enteritidis) (PubMed:30263339, PubMed:22236762). Antibacterial properties of the GBP antimicrobial peptides (AMPs) associated with no cytotoxicity on the viability of human embryonic kidney cells make them promising candidates as natural antibacterial agents (PubMed:22236762, Ref. 12, PubMed:28590128). Fragmented peptides resulting from gastrointestinal digestion of germinated soybeans seem to have anticancer and antiinflammatory actions on human colon cancer cells (e.g. Caco-2, HT-29, and HCT-116) and macrophages (LPS-stimulated RAW 264.7) (PubMed:29037738). Such peptides resulting from digested germinated soybeans exhibit also anti-diabetic potential by inhibiting dipeptidyl peptidase IV (DPP-IV), salivary alpha-amylase and intestinal alpha-glucosidase enzymes (PubMed:30249015).9 Publications
Keywords - Diseasei
AllergenProtein family/group databases
Allergomei | 5821, Gly m 6 5824, Gly m 6.0301 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000032017 | 22 – 296 | Glycinin A1b subunitAdd BLAST | 275 | |
ChainiPRO_0000032018 | 297 – 476 | Glycinin B2 subunitAdd BLAST | 180 | |
PropeptideiPRO_0000032019 | 477 – 481 | 5 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 31 ↔ 64 | By similarity | ||
Disulfide bondi | 107 ↔ 303 | Interchain (between A and B chains)By similarity |
Post-translational modificationi
During soybean germination, seed storage proteins are hydrolyzed by protease/26S proteasome.1 Publication
Keywords - PTMi
Disulfide bondProteomic databases
PRIDEi | P11828 |
Expressioni
Tissue specificityi
Confined to developing seeds.1 Publication
Developmental stagei
Progressive level increase from pod to full-size seed growth.1 Publication
Gene expression databases
Genevisiblei | P11828, GM |
Interactioni
Subunit structurei
Hexamer; each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond.
By similarityProtein-protein interaction databases
STRINGi | 3847.GLYMA19G34780.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 240 | Cupin type-1 1Sequence analysisAdd BLAST | 205 | |
Domaini | 309 – 458 | Cupin type-1 2Sequence analysisAdd BLAST | 150 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 472 – 481 | Vacuolar targeting signalBy similarity | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 169 – 210 | Gln-richPROSITE-ProRule annotationAdd BLAST | 42 | |
Compositional biasi | 245 – 250 | Poly-GluSequence analysis | 6 |
Sequence similaritiesi
Belongs to the 11S seed storage protein (globulins) family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QU1J, Eukaryota |
HOGENOMi | CLU_026341_2_0_1 |
InParanoidi | P11828 |
OMAi | VHGHALM |
OrthoDBi | 603461at2759 |
Family and domain databases
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR022379, 11S_seedstore_CS IPR006044, 11S_seedstore_pln IPR006045, Cupin_1 IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
Pfami | View protein in Pfam PF00190, Cupin_1, 2 hits |
PRINTSi | PR00439, 11SGLOBULIN |
SMARTi | View protein in SMART SM00835, Cupin_1, 2 hits |
SUPFAMi | SSF51182, SSF51182, 1 hit |
PROSITEi | View protein in PROSITE PS00305, 11S_SEED_STORAGE, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: P11828-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAKLVLSLCF LLFSGCCFAF SFREQPQQNE CQIQRLNALK PDNRIESEGG
60 70 80 90 100
FIETWNPNNK PFQCAGVALS RCTLNRNALR RPSYTNAPQE IYIQQGSGIF
110 120 130 140 150
GMIFPGCPST FEEPQQKGQS SRPQDRHQKI YHFREGDLIA VPTGFAYWMY
160 170 180 190 200
NNEDTPVVAV SLIDTNSFQN QLDQMPRRFY LAGNQEQEFL QYQPQKQQGG
210 220 230 240 250
TQSQKGKRQQ EEENEGGSIL SGFAPEFLEH AFVVDRQIVR KLQGENEEEE
260 270 280 290 300
KGAIVTVKGG LSVISPPTEE QQQRPEEEEK PDCDEKDKHC QSQSRNGIDE
310 320 330 340 350
TICTMRLRHN IGQTSSPDIF NPQAGSITTA TSLDFPALSW LKLSAQFGSL
360 370 380 390 400
RKNAMFVPHY NLNANSIIYA LNGRALVQVV NCNGERVFDG ELQEGQVLIV
410 420 430 440 450
PQNFAVAARS QSDNFEYVSF KTNDRPSIGN LAGANSLLNA LPEEVIQQTF
460 470 480
NLRRQQARQV KNNNPFSFLV PPKESQRRVV A
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 42 | D → G in BAC55938 (Ref. 3) Curated | 1 | |
Sequence conflicti | 42 | D → G in BAC55937 (Ref. 3) Curated | 1 | |
Sequence conflicti | 97 | S → N in BAC55938 (Ref. 3) Curated | 1 | |
Sequence conflicti | 145 | F → L in BAC55938 (Ref. 3) Curated | 1 | |
Sequence conflicti | 156 | P → L in BAC55938 (Ref. 3) Curated | 1 | |
Sequence conflicti | 156 | P → L in BAC55937 (Ref. 3) Curated | 1 | |
Sequence conflicti | 162 | L → I in BAC55938 (Ref. 3) Curated | 1 | |
Sequence conflicti | 168 – 169 | FQ → LE in BAC55938 (Ref. 3) Curated | 2 | |
Sequence conflicti | 186 | E → Q in BAC55938 (Ref. 3) Curated | 1 | |
Sequence conflicti | 194 | P → S in BAC55938 (Ref. 3) Curated | 1 | |
Sequence conflicti | 219 | I → M in BAC55938 (Ref. 3) Curated | 1 | |
Sequence conflicti | 219 | I → M in BAC55937 (Ref. 3) Curated | 1 | |
Sequence conflicti | 238 | I → IV in BAC55938 (Ref. 3) Curated | 1 | |
Sequence conflicti | 258 | K → R in BAC55938 (Ref. 3) Curated | 1 | |
Sequence conflicti | 271 – 272 | QQ → RR in BAC55938 (Ref. 3) Curated | 2 | |
Sequence conflicti | 306 | R → G in BAC55938 (Ref. 3) Curated | 1 | |
Sequence conflicti | 399 | I → T in BAC55938 (Ref. 3) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060144 | 72 – 75 | CTLN → YTLI in isoform 2. | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15123 Genomic DNA Translation: CAA33217.1 AB030494 mRNA Translation: BAC55937.1 AB030495 mRNA Translation: BAC55938.1 |
PIRi | S04605 |
RefSeqi | NP_001236840.1, NM_001249911.1 [P11828-1] |
Genome annotation databases
EnsemblPlantsi | KRG95667; KRG95667; GLYMA_19G164900 [P11828-1] |
GeneIDi | 547463 |
Gramenei | KRG95667; KRG95667; GLYMA_19G164900 [P11828-1] |
KEGGi | gmx:547463 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15123 Genomic DNA Translation: CAA33217.1 AB030494 mRNA Translation: BAC55937.1 AB030495 mRNA Translation: BAC55938.1 |
PIRi | S04605 |
RefSeqi | NP_001236840.1, NM_001249911.1 [P11828-1] |
3D structure databases
SMRi | P11828 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3847.GLYMA19G34780.1 |
Protein family/group databases
Allergomei | 5821, Gly m 6 5824, Gly m 6.0301 |
Proteomic databases
PRIDEi | P11828 |
Genome annotation databases
EnsemblPlantsi | KRG95667; KRG95667; GLYMA_19G164900 [P11828-1] |
GeneIDi | 547463 |
Gramenei | KRG95667; KRG95667; GLYMA_19G164900 [P11828-1] |
KEGGi | gmx:547463 |
Phylogenomic databases
eggNOGi | ENOG502QU1J, Eukaryota |
HOGENOMi | CLU_026341_2_0_1 |
InParanoidi | P11828 |
OMAi | VHGHALM |
OrthoDBi | 603461at2759 |
Gene expression databases
Genevisiblei | P11828, GM |
Family and domain databases
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR022379, 11S_seedstore_CS IPR006044, 11S_seedstore_pln IPR006045, Cupin_1 IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
Pfami | View protein in Pfam PF00190, Cupin_1, 2 hits |
PRINTSi | PR00439, 11SGLOBULIN |
SMARTi | View protein in SMART SM00835, Cupin_1, 2 hits |
SUPFAMi | SSF51182, SSF51182, 1 hit |
PROSITEi | View protein in PROSITE PS00305, 11S_SEED_STORAGE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLYG3_SOYBN | |
Accessioni | P11828Primary (citable) accession number: P11828 Secondary accession number(s): Q852U4, Q852U5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | October 1, 1989 | |
Last modified: | August 12, 2020 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families