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Protein

Alcohol dehydrogenase class-3

Gene

ADH5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.1 Publication

Miscellaneous

There are 7 different ADH's isozymes in human: three belongs to class-I: alpha, beta, and gamma, one to class-II: pi, one to class-III: chi, one to class-IV: ADH7 and one to class-V: ADH6.

Catalytic activityi

A primary alcohol + NAD+ = an aldehyde + NADH.1 Publication
A secondary alcohol + NAD+ = a ketone + NADH.1 Publication
S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 2 Zn2+ ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45Zinc 1; catalytic1 Publication1
Metal bindingi67Zinc 1; catalytic1 Publication1
Metal bindingi97Zinc 21 Publication1
Metal bindingi100Zinc 21 Publication1
Metal bindingi103Zinc 21 Publication1
Metal bindingi111Zinc 21 Publication1
Sitei115Important for FDH activity and activation by fatty acids2 Publications1
Metal bindingi174Zinc 1; catalytic1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:HS10601-MONOMER
ReactomeiR-HSA-71384 Ethanol oxidation
SABIO-RKiP11766

Chemistry databases

SwissLipidsiSLP:000000500

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase class-3 (EC:1.1.1.11 Publication)
Alternative name(s):
Alcohol dehydrogenase 5
Alcohol dehydrogenase class chi chain
Alcohol dehydrogenase class-III
Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-)
Short name:
FALDH
Short name:
FDH
Short name:
GSH-FDH
S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.2841 Publication)
Gene namesi
Name:ADH5Imported
Synonyms:ADHX, FDH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000197894.10
HGNCiHGNC:253 ADH5
MIMi103710 gene
neXtProtiNX_P11766

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi115R → A or D: Loss of FDH activity and loss of activation by fatty acids. 2 Publications1

Organism-specific databases

DisGeNETi128
OpenTargetsiENSG00000197894
PharmGKBiPA24574

Chemistry databases

ChEMBLiCHEMBL4116
DrugBankiDB03704 12-Hydroxydodecanoic Acid
DB00898 Ethanol
DB03017 Lauric Acid
DB00157 NADH
DB04153 S-Hydroxymethyl Glutathione

Polymorphism and mutation databases

DMDMi113408

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001607592 – 374Alcohol dehydrogenase class-3Add BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei233N6-succinyllysineBy similarity1
Modified residuei247PhosphoserineCombined sources1
Modified residuei315N6-succinyllysineBy similarity1
Modified residuei324PhosphoserineCombined sources1
Modified residuei351PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP11766
PaxDbiP11766
PeptideAtlasiP11766
PRIDEiP11766
ProteomicsDBi52803

2D gel databases

REPRODUCTION-2DPAGEiIPI00746777

PTM databases

CarbonylDBiP11766
iPTMnetiP11766
PhosphoSitePlusiP11766
SwissPalmiP11766

Expressioni

Gene expression databases

BgeeiENSG00000197894 Expressed in 235 organ(s), highest expression level in lower esophagus
CleanExiHS_ADH5
ExpressionAtlasiP11766 baseline and differential
GenevisibleiP11766 HS

Organism-specific databases

HPAiHPA044578
HPA061919

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi106640, 20 interactors
IntActiP11766, 8 interactors
STRINGi9606.ENSP00000296412

Chemistry databases

BindingDBiP11766

Structurei

Secondary structure

1374
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP11766
SMRiP11766
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11766

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0022 Eukaryota
COG1062 LUCA
GeneTreeiENSGT00430000030800
HOGENOMiHOG000294674
HOVERGENiHBG000195
InParanoidiP11766
KOiK00121
OMAiATHKGWG
OrthoDBiEOG091G08N3
PhylomeDBiP11766
TreeFamiTF300429

Family and domain databases

CDDicd08300 alcohol_DH_class_III, 1 hit
InterProiView protein in InterPro
IPR014183 ADH_3
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SUPFAMiSSF50129 SSF50129, 2 hits
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR02818 adh_III_F_hyde, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

P11766-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MANEVIKCKA AVAWEAGKPL SIEEIEVAPP KAHEVRIKII ATAVCHTDAY
60 70 80 90 100
TLSGADPEGC FPVILGHEGA GIVESVGEGV TKLKAGDTVI PLYIPQCGEC
110 120 130 140 150
KFCLNPKTNL CQKIRVTQGK GLMPDGTSRF TCKGKTILHY MGTSTFSEYT
160 170 180 190 200
VVADISVAKI DPLAPLDKVC LLGCGISTGY GAAVNTAKLE PGSVCAVFGL
210 220 230 240 250
GGVGLAVIMG CKVAGASRII GVDINKDKFA RAKEFGATEC INPQDFSKPI
260 270 280 290 300
QEVLIEMTDG GVDYSFECIG NVKVMRAALE ACHKGWGVSV VVGVAASGEE
310 320 330 340 350
IATRPFQLVT GRTWKGTAFG GWKSVESVPK LVSEYMSKKI KVDEFVTHNL
360 370
SFDEINKAFE LMHSGKSIRT VVKI
Length:374
Mass (Da):39,724
Last modified:January 23, 2007 - v4
Checksum:iF4F823B4A609C952
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YAG8H0YAG8_HUMAN
Alcohol dehydrogenase class-3
ADH5
262Annotation score:
D6R9G2D6R9G2_HUMAN
Alcohol dehydrogenase class-3
ADH5
95Annotation score:
D6RFE4D6RFE4_HUMAN
Alcohol dehydrogenase class-3
ADH5
115Annotation score:
D6RAY0D6RAY0_HUMAN
Alcohol dehydrogenase class-3
ADH5
56Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167D → Y in AAA51597 (PubMed:2679557).Curated1
Sequence conflicti246F → L in AAA51597 (PubMed:2679557).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_025823163L → S1 PublicationCorresponds to variant dbSNP:rs28730623Ensembl.1
Natural variantiVAR_025824309V → I1 PublicationCorresponds to variant dbSNP:rs28730628Ensembl.1
Natural variantiVAR_048199353D → E. Corresponds to variant dbSNP:rs16996593Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30471 mRNA Translation: AAA79018.1
M29872 mRNA Translation: AAA51597.1
M81118
, M81112, M81113, M81114, M81115, M81116, M81117 Genomic DNA Translation: AAA51596.1
CR541689 mRNA Translation: CAG46490.1
BT019832 mRNA Translation: AAV38635.1
AY987960 Genomic DNA Translation: AAX81412.1
BC014665 mRNA Translation: AAH14665.1
CCDSiCCDS47111.1
PIRiJH0789 DEHUC2
RefSeqiNP_000662.3, NM_000671.4
UniGeneiHs.78989

Genome annotation databases

EnsembliENST00000296412; ENSP00000296412; ENSG00000197894
GeneIDi128
KEGGihsa:128

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30471 mRNA Translation: AAA79018.1
M29872 mRNA Translation: AAA51597.1
M81118
, M81112, M81113, M81114, M81115, M81116, M81117 Genomic DNA Translation: AAA51596.1
CR541689 mRNA Translation: CAG46490.1
BT019832 mRNA Translation: AAV38635.1
AY987960 Genomic DNA Translation: AAX81412.1
BC014665 mRNA Translation: AAH14665.1
CCDSiCCDS47111.1
PIRiJH0789 DEHUC2
RefSeqiNP_000662.3, NM_000671.4
UniGeneiHs.78989

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M6HX-ray2.00A/B2-374[»]
1M6WX-ray2.30A/B2-374[»]
1MA0X-ray2.30A/B2-374[»]
1MC5X-ray2.60A/B1-374[»]
1MP0X-ray2.20A/B2-374[»]
1TEHX-ray2.70A/B2-374[»]
2FZEX-ray1.90A/B2-374[»]
2FZWX-ray1.84A/B2-374[»]
3QJ5X-ray1.90A/B2-374[»]
ProteinModelPortaliP11766
SMRiP11766
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106640, 20 interactors
IntActiP11766, 8 interactors
STRINGi9606.ENSP00000296412

Chemistry databases

BindingDBiP11766
ChEMBLiCHEMBL4116
DrugBankiDB03704 12-Hydroxydodecanoic Acid
DB00898 Ethanol
DB03017 Lauric Acid
DB00157 NADH
DB04153 S-Hydroxymethyl Glutathione
SwissLipidsiSLP:000000500

PTM databases

CarbonylDBiP11766
iPTMnetiP11766
PhosphoSitePlusiP11766
SwissPalmiP11766

Polymorphism and mutation databases

DMDMi113408

2D gel databases

REPRODUCTION-2DPAGEiIPI00746777

Proteomic databases

EPDiP11766
PaxDbiP11766
PeptideAtlasiP11766
PRIDEiP11766
ProteomicsDBi52803

Protocols and materials databases

DNASUi128
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296412; ENSP00000296412; ENSG00000197894
GeneIDi128
KEGGihsa:128

Organism-specific databases

CTDi128
DisGeNETi128
EuPathDBiHostDB:ENSG00000197894.10
GeneCardsiADH5
HGNCiHGNC:253 ADH5
HPAiHPA044578
HPA061919
MIMi103710 gene
neXtProtiNX_P11766
OpenTargetsiENSG00000197894
PharmGKBiPA24574
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0022 Eukaryota
COG1062 LUCA
GeneTreeiENSGT00430000030800
HOGENOMiHOG000294674
HOVERGENiHBG000195
InParanoidiP11766
KOiK00121
OMAiATHKGWG
OrthoDBiEOG091G08N3
PhylomeDBiP11766
TreeFamiTF300429

Enzyme and pathway databases

BioCyciMetaCyc:HS10601-MONOMER
ReactomeiR-HSA-71384 Ethanol oxidation
SABIO-RKiP11766

Miscellaneous databases

ChiTaRSiADH5 human
EvolutionaryTraceiP11766
GeneWikiiADH5
GenomeRNAii128
PROiPR:P11766
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000197894 Expressed in 235 organ(s), highest expression level in lower esophagus
CleanExiHS_ADH5
ExpressionAtlasiP11766 baseline and differential
GenevisibleiP11766 HS

Family and domain databases

CDDicd08300 alcohol_DH_class_III, 1 hit
InterProiView protein in InterPro
IPR014183 ADH_3
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SUPFAMiSSF50129 SSF50129, 2 hits
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR02818 adh_III_F_hyde, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiADHX_HUMAN
AccessioniPrimary (citable) accession number: P11766
Secondary accession number(s): Q6FHR2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 204 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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