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Protein

Keratin, type II cytoskeletal 8

Gene

Krt8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.By similarity

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei347Stutter1

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-6805567 Keratinization
R-MMU-6809371 Formation of the cornified envelope

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type II cytoskeletal 8
Alternative name(s):
Cytokeratin endo A
Cytokeratin-8
Short name:
CK-8
Keratin-8
Short name:
K8
Type-II keratin Kb8
Gene namesi
Name:Krt8
Synonyms:Krt2-8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:96705 Krt8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Intermediate filament, Keratin, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000637411 – 490Keratin, type II cytoskeletal 8Add BLAST490

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9Phosphoserine; by PKC/PRKCEBy similarity1
Cross-linki11Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei13PhosphoserineBy similarity1
Modified residuei15PhosphoserineBy similarity1
Modified residuei21PhosphoserineCombined sources1
Modified residuei22PhosphoserineBy similarity1
Modified residuei23Omega-N-methylarginineBy similarity1
Modified residuei24Phosphoserine; by PKC/PRKCEBy similarityCurated1
Modified residuei26PhosphothreonineBy similarity1
Modified residuei27PhosphoserineBy similarity1
Modified residuei32Omega-N-methylarginineBy similarity1
Modified residuei34PhosphoserineBy similarity1
Modified residuei37PhosphoserineBy similarity1
Modified residuei39PhosphoserineBy similarity1
Modified residuei40Omega-N-methylarginineBy similarity1
Modified residuei43PhosphoserineBy similarity1
Modified residuei44PhosphoserineBy similarity1
Modified residuei47PhosphoserineBy similarity1
Modified residuei49Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei49Omega-N-methylarginine; alternateBy similarity1
Modified residuei51PhosphoserineBy similarity1
Modified residuei80Phosphoserine; by MAPK1 Publication1
Modified residuei107N6-malonyllysineBy similarity1
Cross-linki128Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki136Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki203Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki203Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei213N6-acetyllysineBy similarity1
Modified residuei259PhosphoserineCombined sources1
Modified residuei280PhosphoserineBy similarity1
Cross-linki291Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei301N6-acetyllysine; alternateBy similarity1
Cross-linki301Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki310Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei331N6-acetyllysine; alternateBy similarity1
Cross-linki331Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei336PhosphoserineBy similarity1
Cross-linki399Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei406PhosphoserineBy similarity1
Modified residuei410PhosphoserineBy similarity1
Modified residuei416PhosphoserineBy similarity1
Modified residuei423PhosphoserineBy similarity1
Modified residuei430PhosphoserineBy similarity1
Modified residuei432PhosphoserineBy similarity1
Modified residuei438PhosphoserineBy similarity1
Cross-linki479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki479Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei482PhosphoserineCombined sources1
Modified residuei484PhosphoserineBy similarity1
Modified residuei485PhosphoserineCombined sources1
Modified residuei489PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-80 phosphorylation plays an important role in keratin filament reorganization (By similarity).By similarity
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation (By similarity).By similarity
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP11679
PaxDbiP11679
PeptideAtlasiP11679
PRIDEiP11679

2D gel databases

SWISS-2DPAGEiP11679

PTM databases

iPTMnetiP11679
PhosphoSitePlusiP11679

Expressioni

Tissue specificityi

Expressed in abundance in the epithelia of colon, bladder, ileum, and stomach, with lower expression observed in earskin (at protein level). Also expressed in pancreas, liver, dudenum and jejunum.1 Publication

Gene expression databases

BgeeiENSMUSG00000049382 Expressed in 62 organ(s), highest expression level in placenta
CleanExiMM_KRT8
GenevisibleiP11679 MM

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1 (By similarity). Interacts with GPER1 (By similarity). Interacts with EPPK1 (PubMed:25617501).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201037, 8 interactors
IntActiP11679, 3 interactors
MINTiP11679
STRINGi10090.ENSMUSP00000023952

Structurei

3D structure databases

ProteinModelPortaliP11679
SMRiP11679
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 408IF rodPROSITE-ProRule annotationAdd BLAST312

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 96HeadAdd BLAST96
Regioni97 – 132Coil 1AAdd BLAST36
Regioni133 – 149Linker 1Add BLAST17
Regioni150 – 241Coil 1BAdd BLAST92
Regioni242 – 265Linker 12Add BLAST24
Regioni266 – 403Coil 2Add BLAST138
Regioni267 – 387Necessary for interaction with PNNBy similarityAdd BLAST121
Regioni404 – 490TailAdd BLAST87

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 51Ser-richAdd BLAST43

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG4R Eukaryota
ENOG410YY6B LUCA
GeneTreeiENSGT00760000118796
HOVERGENiHBG013015
InParanoidiP11679
KOiK07605
OMAiIQKRTDM
OrthoDBiEOG091G09KR
PhylomeDBiP11679
TreeFamiTF317854

Family and domain databases

InterProiView protein in InterPro
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR032444 Keratin_2_head
IPR003054 Keratin_II
PANTHERiPTHR23239 PTHR23239, 1 hit
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF16208 Keratin_2_head, 1 hit
PRINTSiPR01276 TYPE2KERATIN
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit

Sequencei

Sequence statusi: Complete.

P11679-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSIRVTQKSY KMSTSGPRAF SSRSFTSGPG ARISSSSFSR VGSSSSSFRG
60 70 80 90 100
SMGTGVGLGG FGGAGVGGIT AVTVNQSLLS PLKLEVDPNI QAVRTQEKEQ
110 120 130 140 150
IKSLNNKFAS FIDKVRFLEQ QNKMLETKWS LLQQQKTSRS NMDNMFESYI
160 170 180 190 200
NNLRRQLEAL GQEKLKLEAE LGNMQGLVED FKNKYEDEIN KRTEMENEFV
210 220 230 240 250
LIKKDVDEAY MNKVELESRL EGLTDEINFL RQIHEEEIRE LQSQISDTSV
260 270 280 290 300
VLSMDNSRSL DMDGIIAEVR AQYEDIANRS RAEAETMYQI KYEELQTLAG
310 320 330 340 350
KHGDDLRRTK TEISEMNRNI NRLQAEIEAL KGQRASLEAA IADAEQRGEM
360 370 380 390 400
AIKDAQTKLA ELEAALQRAK QDMARQLREY QELMNVKLAL DIEITTYRKL
410 420 430 440 450
LEGEESRLES GMQNMSIHTK TTSGYSGGLS SSYGGLTSPG FSYGMSSFQP
460 470 480 490
GFGSAGGSNT FSRTTKAVVV KKIETRDGKL VSESSDVVSK
Length:490
Mass (Da):54,565
Last modified:January 23, 2007 - v4
Checksum:iC0C79590F0C28502
GO

Sequence cautioni

The sequence AAA37551 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12 – 35MSTSG…ARISS → NVYSVGLLASARSGSWTGSA STA in AAA37548 (PubMed:2467842).CuratedAdd BLAST24
Sequence conflicti40 – 58RVGSS…TGVGL → GRACQLGSSFGEAWHGVS in AAA37548 (PubMed:2467842).CuratedAdd BLAST19
Sequence conflicti52M → L in AAI06155 (PubMed:15489334).Curated1
Sequence conflicti75N → D in AAA37548 (PubMed:2467842).Curated1
Sequence conflicti83 – 87KLEVD → QLSL in AAA37548 (PubMed:2467842).Curated5
Sequence conflicti108F → L in BAE39554 (PubMed:16141072).Curated1
Sequence conflicti190 – 191NK → QQ in CAA31278 (PubMed:2454862).Curated2
Sequence conflicti312E → G in BAE40567 (PubMed:16141072).Curated1
Sequence conflicti325Missing in CAA31278 (PubMed:2454862).Curated1
Sequence conflicti368R → P in CAA31278 (PubMed:2454862).Curated1
Sequence conflicti385N → K in BAE38980 (PubMed:16141072).Curated1
Sequence conflicti385N → K in BAE39006 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12789 mRNA Translation: CAA31278.1
X15662 Genomic DNA Translation: CAA33697.1
M21836 mRNA Translation: AAA37550.1
M21836 mRNA Translation: AAA37551.1 Different initiation.
M22831 mRNA Translation: AAA37548.1
D90360 Genomic DNA Translation: BAA14375.1
AK077597 mRNA Translation: BAC36888.1
AK145546 mRNA Translation: BAE26499.1
AK145679 mRNA Translation: BAE26585.1
AK145777 mRNA Translation: BAE26646.1
AK146948 mRNA Translation: BAE27557.1
AK147044 mRNA Translation: BAE27630.1
AK166737 mRNA Translation: BAE38980.1
AK166768 mRNA Translation: BAE39006.1
AK166854 mRNA Translation: BAE39072.1
AK166960 mRNA Translation: BAE39143.1
AK166993 mRNA Translation: BAE39172.1
AK167120 mRNA Translation: BAE39268.1
AK167269 mRNA Translation: BAE39382.1
AK167471 mRNA Translation: BAE39554.1
AK168522 mRNA Translation: BAE40401.1
AK168540 mRNA Translation: BAE40418.1
AK168561 mRNA Translation: BAE40434.1
AK168726 mRNA Translation: BAE40567.1
AK168910 mRNA Translation: BAE40723.1
AK169136 mRNA Translation: BAE40915.1
AK169691 mRNA Translation: BAE41308.1
BC094009 mRNA Translation: AAH94009.1
BC106154 mRNA Translation: AAI06155.1
CCDSiCCDS27868.1
PIRiJS0658
JT0407
S05474
RefSeqiNP_112447.2, NM_031170.2
UniGeneiMm.358618

Genome annotation databases

EnsembliENSMUST00000023952; ENSMUSP00000023952; ENSMUSG00000049382
GeneIDi16691
KEGGimmu:16691
UCSCiuc007xui.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12789 mRNA Translation: CAA31278.1
X15662 Genomic DNA Translation: CAA33697.1
M21836 mRNA Translation: AAA37550.1
M21836 mRNA Translation: AAA37551.1 Different initiation.
M22831 mRNA Translation: AAA37548.1
D90360 Genomic DNA Translation: BAA14375.1
AK077597 mRNA Translation: BAC36888.1
AK145546 mRNA Translation: BAE26499.1
AK145679 mRNA Translation: BAE26585.1
AK145777 mRNA Translation: BAE26646.1
AK146948 mRNA Translation: BAE27557.1
AK147044 mRNA Translation: BAE27630.1
AK166737 mRNA Translation: BAE38980.1
AK166768 mRNA Translation: BAE39006.1
AK166854 mRNA Translation: BAE39072.1
AK166960 mRNA Translation: BAE39143.1
AK166993 mRNA Translation: BAE39172.1
AK167120 mRNA Translation: BAE39268.1
AK167269 mRNA Translation: BAE39382.1
AK167471 mRNA Translation: BAE39554.1
AK168522 mRNA Translation: BAE40401.1
AK168540 mRNA Translation: BAE40418.1
AK168561 mRNA Translation: BAE40434.1
AK168726 mRNA Translation: BAE40567.1
AK168910 mRNA Translation: BAE40723.1
AK169136 mRNA Translation: BAE40915.1
AK169691 mRNA Translation: BAE41308.1
BC094009 mRNA Translation: AAH94009.1
BC106154 mRNA Translation: AAI06155.1
CCDSiCCDS27868.1
PIRiJS0658
JT0407
S05474
RefSeqiNP_112447.2, NM_031170.2
UniGeneiMm.358618

3D structure databases

ProteinModelPortaliP11679
SMRiP11679
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201037, 8 interactors
IntActiP11679, 3 interactors
MINTiP11679
STRINGi10090.ENSMUSP00000023952

PTM databases

iPTMnetiP11679
PhosphoSitePlusiP11679

2D gel databases

SWISS-2DPAGEiP11679

Proteomic databases

MaxQBiP11679
PaxDbiP11679
PeptideAtlasiP11679
PRIDEiP11679

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023952; ENSMUSP00000023952; ENSMUSG00000049382
GeneIDi16691
KEGGimmu:16691
UCSCiuc007xui.1 mouse

Organism-specific databases

CTDi3856
MGIiMGI:96705 Krt8

Phylogenomic databases

eggNOGiENOG410IG4R Eukaryota
ENOG410YY6B LUCA
GeneTreeiENSGT00760000118796
HOVERGENiHBG013015
InParanoidiP11679
KOiK07605
OMAiIQKRTDM
OrthoDBiEOG091G09KR
PhylomeDBiP11679
TreeFamiTF317854

Enzyme and pathway databases

ReactomeiR-MMU-6805567 Keratinization
R-MMU-6809371 Formation of the cornified envelope

Miscellaneous databases

PROiPR:P11679
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049382 Expressed in 62 organ(s), highest expression level in placenta
CleanExiMM_KRT8
GenevisibleiP11679 MM

Family and domain databases

InterProiView protein in InterPro
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR032444 Keratin_2_head
IPR003054 Keratin_II
PANTHERiPTHR23239 PTHR23239, 1 hit
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF16208 Keratin_2_head, 1 hit
PRINTSiPR01276 TYPE2KERATIN
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiK2C8_MOUSE
AccessioniPrimary (citable) accession number: P11679
Secondary accession number(s): Q3KQK5
, Q3TGI1, Q3TJE1, Q3TKY7, Q61463, Q61518, Q61519
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 172 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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