UniProtKB - P11623 (RDRP_PLRVW)
Protein
Protein P1-P2
Gene
ORF1/ORF2
Organism
Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV)
Status
Functioni
Precursor from which the RNA-dependent RNA polymerase (RdRp) is probably released. RNA-dependent RNA polymerase plays an essential role in virus replication (Potential).Curated
Catalytic activityi
- EC:2.7.7.48PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 255 | For protease activityPROSITE-ProRule annotation | 1 | |
Active sitei | 286 | For protease activityPROSITE-ProRule annotation | 1 | |
Active sitei | 354 | For protease activityPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- nucleotide binding Source: UniProtKB-KW
- RNA binding Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- serine-type endopeptidase activity Source: InterPro
- serine-type exopeptidase activity Source: InterPro
GO - Biological processi
- transcription, DNA-templated Source: InterPro
- viral RNA genome replication Source: InterPro
Keywordsi
Molecular function | Hydrolase, Multifunctional enzyme, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Serine protease, Transferase |
Biological process | Viral RNA replication |
Ligand | Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Protein P1-P2Cleaved into the following 2 chains: Serine protease (EC:3.4.21.-) Alternative name(s): 69.6 kDa protein |
Gene namesi | ORF Names:ORF1/ORF2 |
Organismi | Potato leafroll virus (strain Potato/Netherlands/Wageningen/1989) (PLrV) |
Taxonomic identifieri | 12048 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Kitrinoviricota › Tolucaviricetes › Tolivirales › Luteoviridae › Polerovirus › |
Virus hosti | Solanum tuberosum (Potato) [TaxID: 4113] |
Proteomesi |
|
Subcellular locationi
- Membrane Curated; Multi-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 121 – 141 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 144 – 164 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 172 – 192 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000222400 | 21 – 1062 | Protein P1-P2Add BLAST | 1042 | |
ChainiPRO_0000390907 | 205 – 399 | Serine proteaseSequence analysisAdd BLAST | 195 | |
ChainiPRO_0000390908 | 400 – 1062 | RNA-directed RNA polymeraseSequence analysisAdd BLAST | 663 |
Post-translational modificationi
Specific enzymatic cleavages in vivo yield mature proteins. The protease probably cleaves itself and releases the RdRp (Potential). Cleavages have been shown in the P1 protein, but since the N-terminus containing the serine protease is shared between P1 and P1-P2, cleavages should also occur within the P1-P2 protein.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 204 – 205 | Cleavage; by viral serine proteaseSequence analysis | 2 | |
Sitei | 399 – 400 | Cleavage; by viral serine proteaseBy similarity | 2 |
Proteomic databases
PRIDEi | P11623 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 207 – 399 | Peptidase S39PROSITE-ProRule annotationAdd BLAST | 193 | |
Domaini | 859 – 974 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 116 |
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixFamily and domain databases
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR018019, Luteovirus_Orf2 IPR009003, Peptidase_S1_PA IPR000382, Peptidase_S39B_luteovirus IPR001795, RNA-dir_pol_luteovirus IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF02122, Peptidase_S39, 1 hit PF02123, RdRP_4, 1 hit |
PRINTSi | PR00913, LVIRUSORF2 |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51868, PEPTIDASE_S39, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketIsoform RNA-directed RNA polymerase (identifier: P11623-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNRFTAYAAL FFIFSLCSTA KEAGFQHPAF NFRGTSTMSA LSGDYSAAPT
60 70 80 90 100
PLYKSWALPS SLNLTTQPPP LLTDRSYYEL VQALISKMRL DCQTVGDMTW
110 120 130 140 150
RHLSEMLFAS WNSVKEVSLK AASVTLWAII SIWFGLYWTL ARLITLFLWT
160 170 180 190 200
FSIEALCLIL LGCITSLIYK GALSLSEHLP VFLFMSPLKI IWRAAFSKRN
210 220 230 240 250
YKNEKAVEGY KGFSVPQKPP KSAVIELQHE NGSHLGYANC IRLYSGENAL
260 270 280 290 300
VTAEHCLEGA FATSLKTGNR IPMSTFFPIF KSARNDISIL VGPPNWEGLL
310 320 330 340 350
SVKGAHFITA DKIGKGPASF YTLEKGEWMC HSATIDGAHH QFVSVLCNTG
360 370 380 390 400
PGYSGTGFWS SKNLLGVLKG FPLEEECNYN VMSVIPSIPG ITSPNYVFES
410 420 430 440 450
TAVKGRVFSD ETVKELEREA SEAVKKLARF KSLTGKNWAN DYDSDEDYGL
460 470 480 490 500
EKEAATNAPA EKTAQTNSAE KTAPSTSAEK TAPTNKPFKW ASGTARQNKR
510 520 530 540 550
QLRHPRRRYK RTTNGQNGRT DHHSYGGENQ SLGDRGEDSE QGVSESPAEA
560 570 580 590 600
QTKQTRKTWR EEQAKQFTSY FDAIYKWGAQ EEGCPPGFRK CGNIPGYYHP
610 620 630 640 650
RTKGETKWGQ KLCQVHPELA DKTAGFGWPK AGFEAELQSL NLQAARWLQR
660 670 680 690 700
AESATIPGAE ARKRVIEKTV EAYRNCITNA PLCSLKSKLD WAGFQQDIRE
710 720 730 740 750
AVQSLELDAG VGIPYIAYGL PTHRGWVEDH KLLPVLTQLT FDRLQKMSEA
760 770 780 790 800
SFEDMSAEEL VQEGLCDPIR LFVKGEPHKQ SKLDEGRYRL IMSVSLVDQL
810 820 830 840 850
VARVLFQNQN KREISLWRSV PSKPGFGLST DTQTAEFLEC LQKVSGAPSV
860 870 880 890 900
EELCANHKEH TRPTDCSGFD WSVAYWMLED DMEVRNRLTF NNTQLTERLR
910 920 930 940 950
AAWLKCIGNS VLCLSDGTLL AQTVPGVQKS GSYNTSSSNS RIRVMAAYHC
960 970 980 990 1000
GADWAMAMGD DALEAPNSDL EEYKTLGFKV EVGRELEFCS HIFRNPTLAV
1010 1020 1030 1040 1050
PVNTNKMLYK LIHGYNPECG NPEVIQNYLA AVFSVLQELR HDRELVAKLH
1060
QWLVPSATTK EH
Note: Produced by -1 ribosomal frameshifting between codons 487 and 488.
Isoform Protein P1 (identifier: P11622-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P11622.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence cautioni
The sequence CAA68796 differs from that shown. Ribosomal frameshifting not described. Translation N-terminally extended.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y07496 Genomic RNA Translation: CAA68796.1 Sequence problems. |
PIRi | S03548, RRVQWA |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y07496 Genomic RNA Translation: CAA68796.1 Sequence problems. |
PIRi | S03548, RRVQWA |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Proteomic databases
PRIDEi | P11623 |
Family and domain databases
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR018019, Luteovirus_Orf2 IPR009003, Peptidase_S1_PA IPR000382, Peptidase_S39B_luteovirus IPR001795, RNA-dir_pol_luteovirus IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF02122, Peptidase_S39, 1 hit PF02123, RdRP_4, 1 hit |
PRINTSi | PR00913, LVIRUSORF2 |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51868, PEPTIDASE_S39, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RDRP_PLRVW | |
Accessioni | P11623Primary (citable) accession number: P11623 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | January 19, 2010 | |
Last modified: | December 2, 2020 | |
This is version 87 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |