UniProtKB - P11607 (AT2A2_PIG)
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
ATP2A2
Functioni
Catalytic activityi
- EC:7.2.2.101 Publication
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 304 | Calcium 1; via carbonyl oxygenCombined sources1 Publication | 1 | |
Metal bindingi | 305 | Calcium 1; via carbonyl oxygenCombined sources1 Publication | 1 | |
Metal bindingi | 307 | Calcium 1; via carbonyl oxygenCombined sources1 Publication | 1 | |
Metal bindingi | 309 | Calcium 1Combined sources1 Publication | 1 | |
Active sitei | 351 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 351 | MagnesiumCombined sources1 Publication | 1 | |
Metal bindingi | 353 | Magnesium; via carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 353 | ATPCombined sources1 Publication | 1 | |
Binding sitei | 442 | ATPCombined sources1 Publication | 1 | |
Binding sitei | 489 | ATPCombined sources1 Publication | 1 | |
Binding sitei | 514 | ATPCombined sources1 Publication | 1 | |
Binding sitei | 559 | ATPBy similarity | 1 | |
Binding sitei | 677 | ATPCombined sources1 Publication | 1 | |
Binding sitei | 683 | ATPBy similarity | 1 | |
Metal bindingi | 702 | MagnesiumCombined sources1 Publication | 1 | |
Binding sitei | 705 | ATPCombined sources1 Publication | 1 | |
Metal bindingi | 767 | Calcium 2Combined sources1 Publication | 1 | |
Metal bindingi | 770 | Calcium 2Combined sources1 Publication | 1 | |
Metal bindingi | 795 | Calcium 1Combined sources1 Publication | 1 | |
Metal bindingi | 798 | Calcium 2Combined sources1 Publication | 1 | |
Metal bindingi | 799 | Calcium 1Combined sources1 Publication | 1 | |
Metal bindingi | 799 | Calcium 2Combined sources1 Publication | 1 | |
Metal bindingi | 907 | Calcium 2By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 624 – 626 | ATPCombined sources1 Publication | 3 |
GO - Molecular functioni
- ATPase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- calcium ion binding Source: Ensembl
- calcium transmembrane transporter activity, phosphorylative mechanism Source: GO_Central
- calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential Source: GO_Central
- enzyme binding Source: Ensembl
- ion channel binding Source: Ensembl
- proton-exporting ATPase activity, phosphorylative mechanism Source: GO_Central
- S100 protein binding Source: Ensembl
GO - Biological processi
- calcium ion transmembrane transport Source: GO_Central
- calcium ion transport from cytosol to endoplasmic reticulum Source: Ensembl
- cardiac muscle hypertrophy in response to stress Source: Ensembl
- cellular calcium ion homeostasis Source: GO_Central
- cellular response to oxidative stress Source: Ensembl
- ER-nucleus signaling pathway Source: Ensembl
- negative regulation of heart contraction Source: Ensembl
- negative regulation of receptor binding Source: Ensembl
- organelle organization Source: Ensembl
- positive regulation of endoplasmic reticulum calcium ion concentration Source: Ensembl
- regulation of calcium ion-dependent exocytosis of neurotransmitter Source: Ensembl
- regulation of cardiac muscle contraction by calcium ion signaling Source: GO_Central
- regulation of the force of heart contraction Source: Ensembl
- sarcoplasmic reticulum calcium ion transport Source: Ensembl
- transition between fast and slow fiber Source: Ensembl
- T-tubule organization Source: Ensembl
Keywordsi
Molecular function | Translocase |
Biological process | Calcium transport, Ion transport, Transport |
Ligand | ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (EC:7.2.2.101 Publication)Short name: SERCA2 Short name: SR Ca(2+)-ATPase 2 Alternative name(s): Calcium pump 2 Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform Endoplasmic reticulum class 1/2 Ca(2+) ATPase |
Gene namesi | Name:ATP2A2 |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:85647, ATP2A2 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein 1 Publication
Other locations
- Sarcoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- sarcoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
- ribbon synapse Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 48 | CytoplasmicCuratedAdd BLAST | 48 | |
Transmembranei | 49 – 69 | Helical; Name=1By similarityAdd BLAST | 21 | |
Topological domaini | 70 – 89 | LumenalCuratedAdd BLAST | 20 | |
Transmembranei | 90 – 110 | Helical; Name=2By similarityAdd BLAST | 21 | |
Topological domaini | 111 – 253 | CytoplasmicCuratedAdd BLAST | 143 | |
Transmembranei | 254 – 273 | Helical; Name=3By similarityAdd BLAST | 20 | |
Topological domaini | 274 – 295 | LumenalCuratedAdd BLAST | 22 | |
Transmembranei | 296 – 313 | Helical; Name=4By similarityAdd BLAST | 18 | |
Topological domaini | 314 – 756 | CytoplasmicCuratedAdd BLAST | 443 | |
Transmembranei | 757 – 776 | Helical; Name=5By similarityAdd BLAST | 20 | |
Topological domaini | 777 – 786 | LumenalCurated | 10 | |
Transmembranei | 787 – 807 | Helical; Name=6By similarityAdd BLAST | 21 | |
Topological domaini | 808 – 827 | CytoplasmicCuratedAdd BLAST | 20 | |
Transmembranei | 828 – 850 | Helical; Name=7By similarityAdd BLAST | 23 | |
Topological domaini | 851 – 896 | LumenalCuratedAdd BLAST | 46 | |
Transmembranei | 897 – 916 | Helical; Name=8By similarityAdd BLAST | 20 | |
Topological domaini | 917 – 929 | CytoplasmicCuratedAdd BLAST | 13 | |
Transmembranei | 930 – 948 | Helical; Name=9By similarityAdd BLAST | 19 | |
Topological domaini | 949 – 963 | LumenalCuratedAdd BLAST | 15 | |
Transmembranei | 964 – 984 | Helical; Name=10By similarityAdd BLAST | 21 | |
Topological domaini | 985 – 1042 | CytoplasmicCuratedAdd BLAST | 58 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, Sarcoplasmic reticulumPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046198 | 1 – 1042 | Sarcoplasmic/endoplasmic reticulum calcium ATPase 2Add BLAST | 1042 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 38 | PhosphoserineBy similarity | 1 | |
Modified residuei | 294 | 3'-nitrotyrosineBy similarity | 1 | |
Modified residuei | 295 | 3'-nitrotyrosineBy similarity | 1 | |
Modified residuei | 441 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 531 | PhosphoserineBy similarity | 1 | |
Modified residuei | 580 | PhosphoserineBy similarity | 1 | |
Modified residuei | 663 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 875 ↔ 887 | Combined sources1 Publication |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Nitration, PhosphoproteinProteomic databases
PeptideAtlasi | P11607 |
PRIDEi | P11607 |
Expressioni
Tissue specificityi
Gene expression databases
ExpressionAtlasi | P11607, baseline and differential |
Interactioni
Subunit structurei
Interacts with sarcolipin (SLN) (By similarity).
Interacts with phospholamban (PLN) (By similarity).
Interacts with myoregulin (MRLN) (By similarity).
Interacts with DWORF (By similarity).
Isoform 1 interacts with TRAM2 (via C-terminus).
Interacts with HAX1.
Interacts with S100A8 and S100A9 (By similarity).
Interacts with SLC35G1 and STIM1.
Interacts with TMEM203.
Interacts with TMEM64 and PDIA3 (By similarity).
Interacts with TMX2.
By similarityBinary interactionsi
Hide detailsP11607
With | #Exp. | IntAct |
---|---|---|
CANX [P27824] from Homo sapiens. | 2 | EBI-8004986,EBI-355947 |
OPRD1 [P41143] from Homo sapiens. | 2 | EBI-8004986,EBI-2624456 |
GO - Molecular functioni
- enzyme binding Source: Ensembl
- ion channel binding Source: Ensembl
- S100 protein binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 1149018, 1 interactor |
IntActi | P11607, 2 interactors |
MINTi | P11607 |
Structurei
Secondary structure
3D structure databases
BMRBi | P11607 |
SMRi | P11607 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 575 – 594 | Interaction with HAX1By similarityAdd BLAST | 20 | |
Regioni | 787 – 807 | Interaction with PLNBy similarityAdd BLAST | 21 | |
Regioni | 788 – 1042 | Interaction with TMEM64 and PDIA3By similarityAdd BLAST | 255 | |
Regioni | 931 – 942 | Interaction with PLNBy similarityAdd BLAST | 12 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
GeneTreei | ENSGT00940000159986 |
InParanoidi | P11607 |
OMAi | DPPTPLW |
OrthoDBi | 100699at2759 |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005782, P-type_ATPase_IIA IPR001757, P_typ_ATPase IPR030332, SERCA1/2 |
PANTHERi | PTHR42861:SF18, PTHR42861:SF18, 1 hit |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01116, ATPase-IIA1_Ca, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MENAHTKTVE EVLGHFGVNE STGLSLEQVK KLKERWGSNE LPAEEGKTLL
60 70 80 90 100
ELVIEQFEDL LVRILLLAAC ISFVLAWFEE GEETITAFVE PFVILLILVA
110 120 130 140 150
NAIVGVWQER NAENAIEALK EYEPEMGKVY RQDRKSVQRI KAKDIVPGDI
160 170 180 190 200
VEIAVGDKVP ADIRLTSIKS TTLRVDQSIL TGESVSVIKH TDPVPDPRAV
210 220 230 240 250
NQDKKNMLFS GTNIAAGKAM GVVVATGVNT EIGKIRDEMV ATEQERTPLQ
260 270 280 290 300
QKLDEFGEQL SKVISLICIA VWIINIGHFN DPVHGGSWIR GAIYYFKIAV
310 320 330 340 350
ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS
360 370 380 390 400
DKTGTLTTNQ MSVCRMFILD KVEGDTCSLN EFTITGSTYA PIGEVHKDDK
410 420 430 440 450
PVKCHQYDGL VELATICALC NDSALDYNEA KGVYEKVGEA TETALTCLVE
460 470 480 490 500
KMNVFDTELK GLSKIERANA CNSVIKQLMK KEFTLEFSRD RKSMSVYCTP
510 520 530 540 550
NKPSRTSMSK MFVKGAPEGV IDRCTHIRVG STKVPMTPGV KQKIMSVIRE
560 570 580 590 600
WGSGSDTLRC LALATHDNPM RREEMNLEDS ANFIKYETNL TFVGCVGMLD
610 620 630 640 650
PPRIEVASSV KLCRQAGIRV IMITGDNKGT AVAICRRIGI FGQDEDVTSK
660 670 680 690 700
AFTGREFDEL NPSAQREACL NARCFARVEP SHKSKIVEFL QSFDEITAMT
710 720 730 740 750
GDGVNDAPAL KKSEIGIAMG SGTAVAKTAS EMVLADDNFS TIVAAVEEGR
760 770 780 790 800
AIYNNMKQFI RYLISSNVGE VVCIFLTAAL GFPEALIPVQ LLWVNLVTDG
810 820 830 840 850
LPATALGFNP PDLDIMNKPP RNPKEPLISG WLFFRYLAIG CYVGAATVGA
860 870 880 890 900
AAWWFIAADG GPRVTFYQLS HFLQCKEDNP DFEGVDCAVF ESPYPMTMAL
910 920 930 940 950
SVLVTIEMCN ALNSLSENQS LLRMPPWENI WLVGSICLSM SLHFLILYVE
960 970 980 990 1000
PLPLIFQITP LNLTQWLMVL KISLPVILMD ETLKFVARNY LEPGKECVQP
1010 1020 1030 1040
ATKSCSFSAC TDGISWPFVL LIMPLVIWVY STDTNFSDMF WS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A5S6HET4 | A0A5S6HET4_PIG | Calcium-transporting ATPase | ATP2A2 | 1,007 | Annotation score: | ||
A0A5S6HSJ6 | A0A5S6HSJ6_PIG | Calcium-transporting ATPase | ATP2A2 | 1,005 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000360 | 994 – 1042 | GKECV…DMFWS → AILE in isoform 2. 1 PublicationAdd BLAST | 49 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15074 mRNA Translation: CAA33170.1 X15073 mRNA Translation: CAA33169.1 X53754 Genomic DNA Translation: CAA37783.1 X53754 Genomic DNA Translation: CAA37784.1 |
PIRi | S04651 S04652 |
RefSeqi | NP_999030.1, NM_213865.1 [P11607-1] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15074 mRNA Translation: CAA33170.1 X15073 mRNA Translation: CAA33169.1 X53754 Genomic DNA Translation: CAA37783.1 X53754 Genomic DNA Translation: CAA37784.1 |
PIRi | S04651 S04652 |
RefSeqi | NP_999030.1, NM_213865.1 [P11607-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5MPM | X-ray | 3.30 | A | 1-993 | [»] | |
6HXB | X-ray | 4.00 | A | 1-993 | [»] | |
BMRBi | P11607 | |||||
SMRi | P11607 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 1149018, 1 interactor |
IntActi | P11607, 2 interactors |
MINTi | P11607 |
Proteomic databases
PeptideAtlasi | P11607 |
PRIDEi | P11607 |
Genome annotation databases
Organism-specific databases
CTDi | 488 |
VGNCi | VGNC:85647, ATP2A2 |
Phylogenomic databases
GeneTreei | ENSGT00940000159986 |
InParanoidi | P11607 |
OMAi | DPPTPLW |
OrthoDBi | 100699at2759 |
Gene expression databases
ExpressionAtlasi | P11607, baseline and differential |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005782, P-type_ATPase_IIA IPR001757, P_typ_ATPase IPR030332, SERCA1/2 |
PANTHERi | PTHR42861:SF18, PTHR42861:SF18, 1 hit |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01116, ATPase-IIA1_Ca, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AT2A2_PIG | |
Accessioni | P11607Primary (citable) accession number: P11607 Secondary accession number(s): P11606, P79426, P79427 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | October 1, 1989 | |
Last modified: | December 2, 2020 | |
This is version 175 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families