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Protein

Protein disulfide-isomerase A3

Gene

Pdia3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Caution

Was originally thought to be a phosphatidyl-inositol 4,5-bisphosphate phosphodiesterase type I (phospholipase C-alpha) then was thought (PubMed:1321829 and PubMed:1330685) to be a thiol protease.1 Publication

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Activity regulationi

Seems to be inhibited by acidic phospholipids.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei57NucleophileBy similarity1
Sitei58Contributes to redox potential valueBy similarity1
Sitei59Contributes to redox potential valueBy similarity1
Active sitei60NucleophileBy similarity1
Sitei119Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei406NucleophileBy similarity1
Sitei407Contributes to redox potential valueBy similarity1
Sitei408Contributes to redox potential valueBy similarity1
Active sitei409NucleophileBy similarity1
Sitei471Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

  • MHC class I protein binding Source: RGD
  • peptidase activity Source: RGD
  • protein disulfide isomerase activity Source: GO_Central
  • protein-disulfide reductase (glutathione) activity Source: RGD

GO - Biological processi

Keywordsi

Molecular functionIsomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase A3 (EC:5.3.4.1)
Alternative name(s):
58 kDa glucose-regulated protein
58 kDa microsomal protein
Short name:
p58
Disulfide isomerase ER-60
Endoplasmic reticulum resident protein 57
Short name:
ER protein 57
Short name:
ERp57
Endoplasmic reticulum resident protein 60
Short name:
ER protein 60
Short name:
ERp60
HIP-70
Q-2
Gene namesi
Name:Pdia3
Synonyms:Erp60, Grp58
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68430 Pdia3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi502 – 505QEDL → AAGL: Failure to prevent secretion from ER. 1 Publication4
Mutagenesisi502 – 505Missing : Failure to prevent secretion from ER. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 241 PublicationAdd BLAST24
ChainiPRO_000003422725 – 505Protein disulfide-isomerase A3Add BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi57 ↔ 60Redox-activePROSITE-ProRule annotation
Disulfide bondi57Interchain (with C-115 in TAPBP); in linked formBy similarity
Modified residuei61N6-methyllysineBy similarity1
Disulfide bondi85 ↔ 92By similarity
Modified residuei129N6-succinyllysineBy similarity1
Modified residuei152N6-acetyllysineBy similarity1
Modified residuei218N6-succinyllysineBy similarity1
Modified residuei252N6-acetyllysineBy similarity1
Modified residuei319PhosphothreonineBy similarity1
Modified residuei362N6-acetyllysineBy similarity1
Disulfide bondi406 ↔ 409Redox-activePROSITE-ProRule annotation
Modified residuei494N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Methylation, Phosphoprotein

Proteomic databases

PaxDbiP11598
PRIDEiP11598

2D gel databases

World-2DPAGEi0004:P11598

PTM databases

CarbonylDBiP11598
iPTMnetiP11598
PhosphoSitePlusiP11598
SwissPalmiP11598

Expressioni

Tissue specificityi

In caput epididymal spermatozoa, detected in the head, mid and principal pieces. In cauda epididymal spermatozoa detected only in the acrosome (at protein level).1 Publication

Gene expression databases

GenevisibleiP11598 RN

Interactioni

Subunit structurei

Subunit of the TAP complex, also known as the peptide loading complex (PLC). Can form disulfide-linked heterodimers with TAPBP. Interacts with ERP27 and CANX (By similarity). Interacts with MZB1 in a calcium-dependent manner (By similarity). Interacts with SERPINA2 and with the S and Z variants of SERPINA1. Interacts with ATP2A2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248111, 4 interactors
IntActiP11598, 6 interactors
MINTiP11598
STRINGi10116.ENSRNOP00000020478

Structurei

3D structure databases

ProteinModelPortaliP11598
SMRiP11598
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 133Thioredoxin 1PROSITE-ProRule annotationAdd BLAST109
Domaini343 – 485Thioredoxin 2PROSITE-ProRule annotationAdd BLAST143

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi502 – 505Prevents secretion from ER4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190 Eukaryota
COG0526 LUCA
HOGENOMiHOG000162459
HOVERGENiHBG005920
InParanoidiP11598
KOiK08056
OrthoDBiEOG091G05J9
PhylomeDBiP11598
TreeFamiTF106382

Family and domain databases

InterProiView protein in InterPro
IPR005788 Disulphide_isomerase
IPR005792 Prot_disulphide_isomerase
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00085 Thioredoxin, 2 hits
SUPFAMiSSF52833 SSF52833, 4 hits
TIGRFAMsiTIGR01130 ER_PDI_fam, 1 hit
TIGR01126 pdi_dom, 2 hits
PROSITEiView protein in PROSITE
PS00194 THIOREDOXIN_1, 2 hits
PS51352 THIOREDOXIN_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P11598-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRFSCLALLP GVALLLASAL LASASDVLEL TDENFESRVS DTGSAGLMLV
60 70 80 90 100
EFFAPWCGHC KRLAPEYEAA ATRLKGIVPL AKVDCTANTN TCNKYGVSGY
110 120 130 140 150
PTLKIFRDGE EAGAYDGPRT ADGIVSHLKK QAGPASVPLR TEDEFKKFIS
160 170 180 190 200
DKDASVVGFF RDLFSDGHSE FLKAASNLRD NYRFAHTNVE SLVKEYDDNG
210 220 230 240 250
EGITIFRPLH LANKFEDKIV AYTEKKMTSG KIKKFIQESI FGLCPHMTED
260 270 280 290 300
NKDLIQGKDL LTAYYDVDYE KNTKGSNYWR NRVMMVAKTF LDAGHKLNFA
310 320 330 340 350
VASRKTFSHE LSDFGLESTT GEIPVVAIRT AKGEKFVMQE EFSRDGKALE
360 370 380 390 400
RFLQEYFDGN LKRYLKSEPI PETNEGPVKV VVAESFDDIV NAEDKDVLIE
410 420 430 440 450
FYAPWCGHCK NLEPKYKELG EKLSKDPNIV IAKMDATAND VPSPYEVKGF
460 470 480 490 500
PTIYFSPANK KLTPKKYEGG RELNDFISYL QREATNPPII QEEKPKKKKK

AQEDL
Length:505
Mass (Da):56,623
Last modified:February 1, 1996 - v2
Checksum:iEAC7F0C0BD4F1471
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0H2UHM5A0A0H2UHM5_RAT
Protein disulfide-isomerase
Pdia3 rCG_27009
510Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 13MRFSC…LPGVA → MPSAALRCSRAWR (PubMed:3398923).CuratedAdd BLAST13
Sequence conflicti98S → T in CAA30916 (PubMed:3398923).Curated1
Sequence conflicti232 – 240IKKFIQESI → SRSLFRKA in CAA30916 (PubMed:3398923).Curated9
Sequence conflicti476F → L in BAA09695 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12355 mRNA Translation: CAA30916.1
D63378 mRNA Translation: BAA09695.1
BC062393 mRNA Translation: AAH62393.1
PIRiA28807
A61354
RefSeqiNP_059015.1, NM_017319.1
UniGeneiRn.11527

Genome annotation databases

GeneIDi29468
KEGGirno:29468
UCSCiRGD:68430 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12355 mRNA Translation: CAA30916.1
D63378 mRNA Translation: BAA09695.1
BC062393 mRNA Translation: AAH62393.1
PIRiA28807
A61354
RefSeqiNP_059015.1, NM_017319.1
UniGeneiRn.11527

3D structure databases

ProteinModelPortaliP11598
SMRiP11598
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248111, 4 interactors
IntActiP11598, 6 interactors
MINTiP11598
STRINGi10116.ENSRNOP00000020478

PTM databases

CarbonylDBiP11598
iPTMnetiP11598
PhosphoSitePlusiP11598
SwissPalmiP11598

2D gel databases

World-2DPAGEi0004:P11598

Proteomic databases

PaxDbiP11598
PRIDEiP11598

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29468
KEGGirno:29468
UCSCiRGD:68430 rat

Organism-specific databases

CTDi2923
RGDi68430 Pdia3

Phylogenomic databases

eggNOGiKOG0190 Eukaryota
COG0526 LUCA
HOGENOMiHOG000162459
HOVERGENiHBG005920
InParanoidiP11598
KOiK08056
OrthoDBiEOG091G05J9
PhylomeDBiP11598
TreeFamiTF106382

Miscellaneous databases

PROiPR:P11598

Gene expression databases

GenevisibleiP11598 RN

Family and domain databases

InterProiView protein in InterPro
IPR005788 Disulphide_isomerase
IPR005792 Prot_disulphide_isomerase
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00085 Thioredoxin, 2 hits
SUPFAMiSSF52833 SSF52833, 4 hits
TIGRFAMsiTIGR01130 ER_PDI_fam, 1 hit
TIGR01126 pdi_dom, 2 hits
PROSITEiView protein in PROSITE
PS00194 THIOREDOXIN_1, 2 hits
PS51352 THIOREDOXIN_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPDIA3_RAT
AccessioniPrimary (citable) accession number: P11598
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: February 1, 1996
Last modified: September 12, 2018
This is version 180 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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