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Protein

Ligninase H2

Gene

GLG4

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Depolymerization of lignin. Catalyzes the C(alpha)-C(beta) cleavage of the propyl side chains of lignin.1 Publication

Catalytic activityi

1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H2O2 = 3,4-dimethoxybenzaldehyde + 2-methoxyphenol + glycolaldehyde + H2O.1 Publication
2 (3,4-dimethoxyphenyl)methanol + H2O2 = 2 (3,4-dimethoxyphenyl)methanol radical + 2 H2O.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+Note: Binds 2 calcium ions per subunit.

Kineticsi

  1. KM=85 µM for H2O21 Publication
  2. KM=171 µM for (3,4-dimethoxyphenyl)methanol1 Publication

    pH dependencei

    Optimum pH is 2.3.1 Publication

    Pathwayi: lignin degradation

    This protein is involved in the pathway lignin degradation, which is part of Secondary metabolite metabolism.
    View all proteins of this organism that are known to be involved in the pathway lignin degradation and in Secondary metabolite metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei72Transition state stabilizer1
    Active sitei76Proton acceptor1
    Metal bindingi77Calcium 11
    Metal bindingi95Calcium 1; via carbonyl oxygen1
    Metal bindingi97Calcium 11
    Metal bindingi99Calcium 11
    Metal bindingi205Iron (heme axial ligand)1
    Metal bindingi206Calcium 21
    Metal bindingi223Calcium 21
    Metal bindingi225Calcium 21
    Metal bindingi228Calcium 2; via carbonyl oxygen1
    Metal bindingi230Calcium 21

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase, Peroxidase
    Biological processHydrogen peroxide, Lignin degradation
    LigandCalcium, Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14340
    UniPathwayi
    UPA00892

    Protein family/group databases

    CAZyiAA2 Auxiliary Activities 2
    mycoCLAPiLPO2B_PHACH
    PeroxiBasei2414 PcLiP04_BKMF1767

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ligninase H2 (EC:1.11.1.141 Publication)
    Alternative name(s):
    Diarylpropane peroxidase
    LG4
    Lignin peroxidase
    Gene namesi
    Name:GLG4
    Synonyms:LIP2
    OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
    Taxonomic identifieri5306 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPhanerochaetaceaePhanerochaete

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 21Sequence analysisAdd BLAST21
    PropeptideiPRO_000002376622 – 282 Publications7
    ChainiPRO_000002376729 – 372Ligninase H2Add BLAST344

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi31 ↔ 44
    Disulfide bondi43 ↔ 314
    Disulfide bondi63 ↔ 149
    Disulfide bondi278 ↔ 344
    Glycosylationi286N-linked (GlcNAc...) asparagine1 Publication1

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

    PTM databases

    iPTMnetiP11542

    Expressioni

    Developmental stagei

    Ligninases are expressed during secondary metabolism, and are triggered by nutrient limitation.

    Structurei

    Secondary structure

    1372
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP11542
    SMRiP11542
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP11542

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peroxidase family. Ligninase subfamily.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410II1B Eukaryota
    ENOG410ZS1R LUCA

    Family and domain databases

    CDDicd00692 ligninase, 1 hit
    InterProiView protein in InterPro
    IPR010255 Haem_peroxidase
    IPR002016 Haem_peroxidase_pln/fun/bac
    IPR001621 Ligninase
    IPR024589 Ligninase_C
    IPR019794 Peroxidases_AS
    IPR019793 Peroxidases_heam-ligand_BS
    PfamiView protein in Pfam
    PF00141 peroxidase, 1 hit
    PF11895 Peroxidase_ext, 1 hit
    PRINTSiPR00462 LIGNINASE
    PR00458 PEROXIDASE
    SUPFAMiSSF48113 SSF48113, 1 hit
    PROSITEiView protein in PROSITE
    PS00435 PEROXIDASE_1, 1 hit
    PS00436 PEROXIDASE_2, 1 hit
    PS50873 PEROXIDASE_4, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P11542-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MAFKQLLAAL SVALTLQVTQ AAPNLDKRVA CPDGVHTASN AACCAWFPVL
    60 70 80 90 100
    DDIQQNLFHG GQCGAEAHEA LRMVFHDSIA ISPKLQSQGK FGGGGADGSI
    110 120 130 140 150
    ITFSSIETTY HPNIGLDEVV AIQKPFIAKH GVTRGDFIAF AGAVGVSNCP
    160 170 180 190 200
    GAPQMQFFLG RPEATQAAPD GLVPEPFHTI DQVLARMLDA GGFDEIETVW
    210 220 230 240 250
    LLSAHSIAAA NDVDPTISGL PFDSTPGQFD SQFFVETQLR GTAFPGKTGI
    260 270 280 290 300
    QGTVMSPLKG EMRLQTDHLF ARDSRTACEW QSFVNNQTKL QEDFQFIFTA
    310 320 330 340 350
    LSTLGHDMNA MTDCSEVIPA PKPVNFGPSF FPAGKTHADI EQACASTPFP
    360 370
    TLITAPGPSA SVARIPPPPS PN
    Length:372
    Mass (Da):39,523
    Last modified:February 1, 1991 - v2
    Checksum:iA17AD0AB5FE1A290
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti312T → I in AAA33733 (PubMed:3440521).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X15599 Genomic DNA Translation: CAA33621.1
    M18743 mRNA Translation: AAA33733.1
    PIRiS06043 OPJGH2

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X15599 Genomic DNA Translation: CAA33621.1
    M18743 mRNA Translation: AAA33733.1
    PIRiS06043 OPJGH2

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1QPAX-ray1.80A/B29-372[»]
    ProteinModelPortaliP11542
    SMRiP11542
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    CAZyiAA2 Auxiliary Activities 2
    mycoCLAPiLPO2B_PHACH
    PeroxiBasei2414 PcLiP04_BKMF1767

    PTM databases

    iPTMnetiP11542

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Phylogenomic databases

    eggNOGiENOG410II1B Eukaryota
    ENOG410ZS1R LUCA

    Enzyme and pathway databases

    UniPathwayi
    UPA00892

    BioCyciMetaCyc:MONOMER-14340

    Miscellaneous databases

    EvolutionaryTraceiP11542

    Family and domain databases

    CDDicd00692 ligninase, 1 hit
    InterProiView protein in InterPro
    IPR010255 Haem_peroxidase
    IPR002016 Haem_peroxidase_pln/fun/bac
    IPR001621 Ligninase
    IPR024589 Ligninase_C
    IPR019794 Peroxidases_AS
    IPR019793 Peroxidases_heam-ligand_BS
    PfamiView protein in Pfam
    PF00141 peroxidase, 1 hit
    PF11895 Peroxidase_ext, 1 hit
    PRINTSiPR00462 LIGNINASE
    PR00458 PEROXIDASE
    SUPFAMiSSF48113 SSF48113, 1 hit
    PROSITEiView protein in PROSITE
    PS00435 PEROXIDASE_1, 1 hit
    PS00436 PEROXIDASE_2, 1 hit
    PS50873 PEROXIDASE_4, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiLIG4_PHACH
    AccessioniPrimary (citable) accession number: P11542
    Secondary accession number(s): P14153
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: February 1, 1991
    Last modified: November 22, 2017
    This is version 123 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
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