UniProtKB - P11483 (ACM3_PIG)
Protein
Muscarinic acetylcholine receptor M3
Gene
CHRM3
Organism
Sus scrofa (Pig)
Status
Functioni
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
GO - Molecular functioni
- acetylcholine binding Source: UniProtKB
- G protein-coupled acetylcholine receptor activity Source: UniProtKB
- G protein-coupled receptor activity Source: GO_Central
- G protein-coupled serotonin receptor activity Source: GO_Central
- neurotransmitter receptor activity Source: GO_Central
GO - Biological processi
- adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway Source: GO_Central
- chemical synaptic transmission Source: GO_Central
- G protein-coupled acetylcholine receptor signaling pathway Source: UniProtKB
- G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
- positive regulation of smooth muscle contraction Source: InterPro
- regulation of vascular associated smooth muscle contraction Source: GO_Central
- saliva secretion Source: InterPro
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Names & Taxonomyi
Protein namesi | Recommended name: Muscarinic acetylcholine receptor M3 |
Gene namesi | Name:CHRM3 |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
- postsynaptic cell membrane By similarity; Multi-pass membrane protein Sequence analysis
- Basolateral cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
Note: Colocalizes with TMEM147 in the endoplasmic reticulum (ER) membrane. TMEM147 impairs its trafficking to the cell membrane leading to its retention in the ER membrane.By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Plasma Membrane
- basolateral plasma membrane Source: UniProtKB-SubCell
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
- postsynaptic membrane Source: UniProtKB-SubCell
Other locations
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 67 | ExtracellularBy similarityAdd BLAST | 67 | |
Transmembranei | 68 – 91 | Helical; Name=1By similarityAdd BLAST | 24 | |
Topological domaini | 92 – 104 | CytoplasmicBy similarityAdd BLAST | 13 | |
Transmembranei | 105 – 130 | Helical; Name=2By similarityAdd BLAST | 26 | |
Topological domaini | 131 – 142 | ExtracellularBy similarityAdd BLAST | 12 | |
Transmembranei | 143 – 164 | Helical; Name=3By similarityAdd BLAST | 22 | |
Topological domaini | 165 – 184 | CytoplasmicBy similarityAdd BLAST | 20 | |
Transmembranei | 185 – 206 | Helical; Name=4By similarityAdd BLAST | 22 | |
Topological domaini | 207 – 229 | ExtracellularBy similarityAdd BLAST | 23 | |
Transmembranei | 230 – 252 | Helical; Name=5By similarityAdd BLAST | 23 | |
Topological domaini | 253 – 491 | CytoplasmicBy similarityAdd BLAST | 239 | |
Transmembranei | 492 – 514 | Helical; Name=6By similarityAdd BLAST | 23 | |
Topological domaini | 515 – 526 | ExtracellularBy similarityAdd BLAST | 12 | |
Transmembranei | 527 – 546 | Helical; Name=7By similarityAdd BLAST | 20 | |
Topological domaini | 547 – 590 | CytoplasmicBy similarityAdd BLAST | 44 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Endoplasmic reticulum, Membrane, Postsynaptic cell membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069032 | 1 – 590 | Muscarinic acetylcholine receptor M3Add BLAST | 590 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 6 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 7 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 15 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 41 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 48 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 53 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 141 ↔ 221 | PROSITE-ProRule annotation | ||
Modified residuei | 385 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 517 ↔ 520 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PRIDEi | P11483 |
Interactioni
Subunit structurei
Homodimer; the dimers can form tetramers (By similarity).
Interacts with NALCN (By similarity).
Interacts with TMEM147 (By similarity).
By similarityChemistry databases
BindingDBi | P11483 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 148 – 152 | Agonist bindingBy similarity | 5 | |
Regioni | 507 – 530 | Agonist bindingBy similarityAdd BLAST | 24 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 275 – 281 | Basolateral sorting signalBy similarity | 7 |
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
InParanoidi | P11483 |
OrthoDBi | 1245472at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001183, Musac_Ach_M3_rcpt IPR000995, Musac_Ach_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00243, MUSCARINICR PR00540, MUSCRINICM3R |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P11483-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLHNNNTTS PLFPNISSSW IHGPSDAGLP PGTVTHFGSY NISQAAGNFS
60 70 80 90 100
SPNGTTSDPL GGHTIWQVVF IAFLTGILAL VTIIGNILVI VAFKVNKQLK
110 120 130 140 150
TVNNYFLLSL ACADLIIGVI SMNLFTTYII MNRWALGNLA CDLWLSIDYV
160 170 180 190 200
ASNASVMNLL VISFDRYFSI TRPLTYRAKR TTKRAGVMIG LAWVISFILW
210 220 230 240 250
APAILFWQYF VGKRTVPPGE CFIQFLSEPT ITFGTAIAAF YMPVTIMTIL
260 270 280 290 300
YWRIYKETEK RTKELAGLQA SGTEAEAENF VHPTGSSRSC SSYELQQQSL
310 320 330 340 350
KRSARRKYGR CHFWFTTKSW KPSAEQMDQD HSSSDSWNNN DAAASLENSA
360 370 380 390 400
SSDEEDIGSE TRAIYSIVLK LPGHSTILNS TKLPSSDNLQ VPEEELGTVD
410 420 430 440 450
LERKASKLQA QKSMDDGGSF QKSFSKLPIQ LESAVDTAKA SDVNSSVGKT
460 470 480 490 500
TATLPLSFKE ATLAKRFALK TRSQITKRKR MSLIKEKKAA QTLSAILLAF
510 520 530 540 550
IITWTPYNIM VLVNTFCDSC IPKTYWNLGY WLCYINSTVN PVCYALCNKT
560 570 580 590
FRTTFKMLLL CQCDKRKRRK QQYQQRQSVI FHKRVPEQAL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12712 Genomic DNA Translation: CAA31215.1 |
PIRi | S01114 |
RefSeqi | NP_001116570.1, NM_001123098.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X12712 Genomic DNA Translation: CAA31215.1 |
PIRi | S01114 |
RefSeqi | NP_001116570.1, NM_001123098.1 |
3D structure databases
SMRi | P11483 |
ModBasei | Search... |
Chemistry databases
BindingDBi | P11483 |
Protein family/group databases
GPCRDBi | Search... |
Proteomic databases
PRIDEi | P11483 |
Genome annotation databases
Organism-specific databases
CTDi | 1131 |
Phylogenomic databases
InParanoidi | P11483 |
OrthoDBi | 1245472at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001183, Musac_Ach_M3_rcpt IPR000995, Musac_Ach_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00243, MUSCARINICR PR00540, MUSCRINICM3R |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACM3_PIG | |
Accessioni | P11483Primary (citable) accession number: P11483 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | October 1, 1989 | |
Last modified: | February 10, 2021 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - SIMILARITY comments
Index of protein domains and families