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Protein

Enterobactin synthase component F

Gene

entF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates the carboxylate group of L-serine via ATP-dependent PPi exchange reactions to the aminoacyladenylate, preparing that molecule for the final stages of enterobactin synthesis. Holo-EntF acts as the catalyst for the formation of the three amide and three ester bonds present in the cyclic (2,3-dihydroxybenzoyl)serine trimer enterobactin, using seryladenylate and acyl-holo-EntB (acylated with 2,3-dihydroxybenzoate by EntE).

Catalytic activityi

ATP + L-serine = diphosphate + L-serine-adenylate.

Cofactori

pantetheine 4'-phosphateNote: Binds 1 phosphopantetheine covalently.

Pathwayi: enterobactin biosynthesis

This protein is involved in the pathway enterobactin biosynthesis, which is part of Siderophore biosynthesis.
View all proteins of this organism that are known to be involved in the pathway enterobactin biosynthesis and in Siderophore biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • amino acid activation for nonribosomal peptide biosynthetic process Source: EcoCyc
  • enterobactin biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionLigase, Multifunctional enzyme, Transferase
Biological processEnterobactin biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ENTF-MONOMER
MetaCyc:ENTF-MONOMER
BRENDAi6.3.2.14 2026
UniPathwayi
UPA00017

Protein family/group databases

ESTHERiecoli-entf Thioesterase

Names & Taxonomyi

Protein namesi
Recommended name:
Enterobactin synthase component F (EC:2.7.7.-)
Alternative name(s):
Enterochelin synthase F
Serine-activating enzyme
Seryl-AMP ligase
Gene namesi
Name:entF
Ordered Locus Names:b0586, JW0578
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10264 entF

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1138S → A: No enterobactin synthase activity. This mutant holo-EntF is still able to adenylate serine and to autoacylate itself with serine. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001930771 – 1293Enterobactin synthase component FAdd BLAST1293

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1006O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1

Post-translational modificationi

4'-phosphopantetheine is transferred from CoA to a specific serine of apo-EntF by EntD. Holo-EntF so formed is then acylated with seryl-AMP.

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

EPDiP11454
PaxDbiP11454
PRIDEiP11454

Expressioni

Inductioni

Transcriptionally regulated by iron and the fur protein.

Interactioni

Subunit structurei

EntB, EntD, EntE, and EntF form a multienzyme complex called enterobactin synthase. EntF acts as a catalytic monomer.

Protein-protein interaction databases

BioGridi4259895, 304 interactors
DIPiDIP-9516N
IntActiP11454, 10 interactors
STRINGi316385.ECDH10B_0654

Structurei

Secondary structure

11293
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP11454
SMRiP11454
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP11454

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini971 – 1046CarrierPROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 301Elongation/condensationAdd BLAST301
Regioni482 – 887AdenylationAdd BLAST406
Regioni1066 – 1293ThioesteraseAdd BLAST228

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C0W Bacteria
COG1020 LUCA
COG3319 LUCA
HOGENOMiHOG000229993
InParanoidiP11454
KOiK02364
PhylomeDBiP11454

Family and domain databases

Gene3Di3.30.559.10, 1 hit
3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR010071 AA_adenyl_domain
IPR029058 AB_hydrolase
IPR036736 ACP-like_sf
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR023213 CAT-like_dom_sf
IPR001242 Condensatn
IPR020806 PKS_PP-bd
IPR020802 PKS_thioesterase
IPR009081 PP-bd_ACP
IPR006162 Ppantetheine_attach_site
IPR001031 Thioesterase
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PF00668 Condensation, 1 hit
PF00550 PP-binding, 1 hit
PF00975 Thioesterase, 1 hit
SMARTiView protein in SMART
SM00823 PKS_PP, 1 hit
SM00824 PKS_TE, 1 hit
SUPFAMiSSF47336 SSF47336, 1 hit
SSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR01733 AA-adenyl-dom, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
PS50075 CARRIER, 1 hit
PS00012 PHOSPHOPANTETHEINE, 1 hit

Sequencei

Sequence statusi: Complete.

P11454-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSQHLPLVAA QPGIWMAEKL SELPSAWSVA HYVELTGEVD SPLLARAVVA
60 70 80 90 100
GLAQADTLRM RFTEDNGEVW QWVDDALTFE LPEIIDLRTN IDPHGTAQAL
110 120 130 140 150
MQADLQQDLR VDSGKPLVFH QLIQVADNRW YWYQRYHHLL VDGFSFPAIT
160 170 180 190 200
RQIANIYCTW LRGEPTPASP FTPFADVVEE YQQYRESEAW QRDAAFWAEQ
210 220 230 240 250
RRQLPPPASL SPAPLPGRSA SADILRLKLE FTDGEFRQLA TQLSGVQRTD
260 270 280 290 300
LALALAALWL GRLCNRMDYA AGFIFMRRLG SAALTATGPV LNVLPLGIHI
310 320 330 340 350
AAQETLPELA TRLAAQLKKM RRHQRYDAEQ IVRDSGRAAG DEPLFGPVLN
360 370 380 390 400
IKVFDYQLDI PDVQAQTHTL ATGPVNDLEL ALFPDVHGDL SIEILANKQR
410 420 430 440 450
YDEPTLIQHA ERLKMLIAQF AADPALLCGD VDIMLPGEYA QLAQLNATQV
460 470 480 490 500
EIPETTLSAL VAEQAAKTPD APALADARYL FSYREMREQV VALANLLRER
510 520 530 540 550
GVKPGDSVAV ALPRSVFLTL ALHAIVEAGA AWLPLDTGYP DDRLKMMLED
560 570 580 590 600
ARPSLLITTD DQLPRFSDVP NLTSLCYNAP LTPQGSAPLQ LSQPHHTAYI
610 620 630 640 650
IFTSGSTGRP KGVMVGQTAI VNRLLWMQNH YPLTGEDVVA QKTPCSFDVS
660 670 680 690 700
VWEFFWPFIA GAKLVMAEPE AHRDPLAMQQ FFAEYGVTTT HFVPSMLAAF
710 720 730 740 750
VASLTPQTAR QSCATLKQVF CSGEALPADL CREWQQLTGA PLHNLYGPTE
760 770 780 790 800
AAVDVSWYPA FGEELAQVRG SSVPIGYPVW NTGLRILDAM MHPVPPGVAG
810 820 830 840 850
DLYLTGIQLA QGYLGRPDLT ASRFIADPFA PGERMYRTGD VARWLDNGAV
860 870 880 890 900
EYLGRSDDQL KIRGQRIELG EIDRVMQALP DVEQAVTHAC VINQAAATGG
910 920 930 940 950
DARQLVGYLV SQSGLPLDTS ALQAQLRETL PPHMVPVVLL QLPQLPLSAN
960 970 980 990 1000
GKLDRKALPL PELKAQAPGR APKAGSETII AAAFSSLLGC DVQDADADFF
1010 1020 1030 1040 1050
ALGGHSLLAM KLAAQLSRQV ARQVTPGQVM VASTVAKLAT IIDAEEDSTR
1060 1070 1080 1090 1100
RMGFETILPL REGNGPTLFC FHPASGFAWQ FSVLSRYLDP QWSIIGIQSP
1110 1120 1130 1140 1150
RPNGPMQTAA NLDEVCEAHL ATLLEQQPHG PYYLLGYSLG GTLAQGIAAR
1160 1170 1180 1190 1200
LRARGEQVAF LGLLDTWPPE TQNWQEKEAN GLDPEVLAEI NREREAFLAA
1210 1220 1230 1240 1250
QQGSTSTELF TTIEGNYADA VRLLTTAHSV PFDGKATLFV AERTLQEGMS
1260 1270 1280 1290
PERAWSPWIA ELDIYRQDCA HVDIISPGTF EKIGPIIRAT LNR
Length:1,293
Mass (Da):141,991
Last modified:November 1, 1997 - v3
Checksum:iF3FCBD3836A39358
GO

Sequence cautioni

The sequence AAB40785 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti441 – 442QL → HV in AAA92015 (PubMed:1826089).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60177 Genomic DNA Translation: AAA92015.1
U82598 Genomic DNA Translation: AAB40785.1 Different initiation.
U00096 Genomic DNA Translation: AAC73687.1
AP009048 Genomic DNA Translation: BAE76341.1
M17354 Genomic DNA Translation: AAA23727.1
J04216 Genomic DNA Translation: AAA23759.1
PIRiH64791 YGECEF
RefSeqiNP_415118.1, NC_000913.3
WP_000077805.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73687; AAC73687; b0586
BAE76341; BAE76341; BAE76341
GeneIDi945184
KEGGiecj:JW0578
eco:b0586
PATRICifig|1411691.4.peg.1684

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60177 Genomic DNA Translation: AAA92015.1
U82598 Genomic DNA Translation: AAB40785.1 Different initiation.
U00096 Genomic DNA Translation: AAC73687.1
AP009048 Genomic DNA Translation: BAE76341.1
M17354 Genomic DNA Translation: AAA23727.1
J04216 Genomic DNA Translation: AAA23759.1
PIRiH64791 YGECEF
RefSeqiNP_415118.1, NC_000913.3
WP_000077805.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ROQNMR-A960-1293[»]
3TEJX-ray1.90A/B965-1293[»]
5JA1X-ray3.00A1-1293[»]
5JA2X-ray3.00A1-1293[»]
5T3DX-ray2.80A1-1293[»]
ProteinModelPortaliP11454
SMRiP11454
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259895, 304 interactors
DIPiDIP-9516N
IntActiP11454, 10 interactors
STRINGi316385.ECDH10B_0654

Protein family/group databases

ESTHERiecoli-entf Thioesterase

Proteomic databases

EPDiP11454
PaxDbiP11454
PRIDEiP11454

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73687; AAC73687; b0586
BAE76341; BAE76341; BAE76341
GeneIDi945184
KEGGiecj:JW0578
eco:b0586
PATRICifig|1411691.4.peg.1684

Organism-specific databases

EchoBASEiEB0260
EcoGeneiEG10264 entF

Phylogenomic databases

eggNOGiENOG4105C0W Bacteria
COG1020 LUCA
COG3319 LUCA
HOGENOMiHOG000229993
InParanoidiP11454
KOiK02364
PhylomeDBiP11454

Enzyme and pathway databases

UniPathwayi
UPA00017

BioCyciEcoCyc:ENTF-MONOMER
MetaCyc:ENTF-MONOMER
BRENDAi6.3.2.14 2026

Miscellaneous databases

EvolutionaryTraceiP11454
PROiPR:P11454

Family and domain databases

Gene3Di3.30.559.10, 1 hit
3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR010071 AA_adenyl_domain
IPR029058 AB_hydrolase
IPR036736 ACP-like_sf
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR023213 CAT-like_dom_sf
IPR001242 Condensatn
IPR020806 PKS_PP-bd
IPR020802 PKS_thioesterase
IPR009081 PP-bd_ACP
IPR006162 Ppantetheine_attach_site
IPR001031 Thioesterase
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PF00668 Condensation, 1 hit
PF00550 PP-binding, 1 hit
PF00975 Thioesterase, 1 hit
SMARTiView protein in SMART
SM00823 PKS_PP, 1 hit
SM00824 PKS_TE, 1 hit
SUPFAMiSSF47336 SSF47336, 1 hit
SSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR01733 AA-adenyl-dom, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
PS50075 CARRIER, 1 hit
PS00012 PHOSPHOPANTETHEINE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiENTF_ECOLI
AccessioniPrimary (citable) accession number: P11454
Secondary accession number(s): P75723, P77095, Q2MBL5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 177 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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