UniProtKB - P11442 (CLH1_RAT)
Clathrin heavy chain 1
Cltc
Functioni
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network (By similarity).
Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577,PubMed:16968737).
The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (By similarity).
Plays a role in early autophagosome formation (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- ankyrin binding Source: RGD
- clathrin light chain binding Source: RGD
- disordered domain specific binding Source: RGD
- double-stranded RNA binding Source: RGD
- heat shock protein binding Source: RGD
- low-density lipoprotein particle receptor binding Source: RGD
- peptide binding Source: RGD
- protein C-terminus binding Source: RGD
- protein kinase binding Source: RGD
- structural molecule activity Source: InterPro
- ubiquitin-specific protease binding Source: RGD
GO - Biological processi
- amyloid-beta clearance by transcytosis Source: RGD
- autophagy Source: UniProtKB-KW
- clathrin coat assembly Source: RGD
- clathrin-dependent endocytosis Source: RGD
- Golgi organization Source: RGD
- intracellular protein transport Source: InterPro
- mitotic cell cycle Source: UniProtKB
- negative regulation of hyaluronan biosynthetic process Source: UniProtKB
- negative regulation of protein localization to plasma membrane Source: RGD
- receptor internalization Source: RGD
- receptor-mediated endocytosis Source: UniProtKB
- regulation of mitotic spindle organization Source: RGD
- retrograde transport, endosome to Golgi Source: RGD
- synaptic vesicle endocytosis Source: SynGO
- transferrin transport Source: RGD
Keywordsi
Biological process | Autophagy |
Enzyme and pathway databases
Reactomei | R-RNO-177504, Retrograde neurotrophin signalling R-RNO-190873, Gap junction degradation R-RNO-196025, Formation of annular gap junctions R-RNO-2132295, MHC class II antigen presentation R-RNO-432720, Lysosome Vesicle Biogenesis R-RNO-432722, Golgi Associated Vesicle Biogenesis R-RNO-437239, Recycling pathway of L1 R-RNO-5099900, WNT5A-dependent internalization of FZD4 R-RNO-5140745, WNT5A-dependent internalization of FZD2, FZD5 and ROR2 R-RNO-8856825, Cargo recognition for clathrin-mediated endocytosis R-RNO-8856828, Clathrin-mediated endocytosis R-RNO-8866427, VLDLR internalisation and degradation R-RNO-8964038, LDL clearance R-RNO-9013420, RHOU GTPase cycle R-RNO-9013424, RHOV GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Clathrin heavy chain 1 |
Gene namesi | Name:Cltc |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2364, Cltc |
Subcellular locationi
Cytoskeleton
- spindle 1 Publication
Other locations
Note: Cytoplasmic face of coated pits and vesicles. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles.By similarity
Cytoskeleton
- mitotic spindle microtubule Source: RGD
- spindle Source: UniProtKB
Cytosol
- cytosol Source: GOC
Golgi apparatus
- clathrin coat of trans-Golgi network vesicle Source: InterPro
Plasma Membrane
- clathrin coat of coated pit Source: InterPro
- clathrin-coated pit Source: RGD
- presynaptic endocytic zone membrane Source: RGD
- sarcolemma Source: RGD
- T-tubule Source: RGD
Other locations
- clathrin coat Source: RGD
- clathrin complex Source: RGD
- clathrin-coated endocytic vesicle Source: GO_Central
- clathrin-coated endocytic vesicle membrane Source: Reactome
- clathrin-coated vesicle Source: RGD
- extrinsic component of synaptic vesicle membrane Source: SynGO
- melanosome Source: UniProtKB-SubCell
- membrane Source: RGD
- membrane coat Source: RGD
- photoreceptor ribbon synapse Source: RGD
- protein-containing complex Source: RGD
- terminal bouton Source: ParkinsonsUK-UCL
Keywords - Cellular componenti
Coated pit, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000205780 | 2 – 1675 | Clathrin heavy chain 1Add BLAST | 1674 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 67 | PhosphoserineBy similarity | 1 | |
Modified residuei | 105 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 184 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 394 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 634 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 737 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 856 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 899 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 1167 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1206 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 1229 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1441 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 1441 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 1477 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 1487 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 1494 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1501 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P11442 |
PaxDbi | P11442 |
PRIDEi | P11442 |
2D gel databases
World-2DPAGEi | 0004:P11442 |
PTM databases
iPTMneti | P11442 |
PhosphoSitePlusi | P11442 |
Interactioni
Subunit structurei
Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat (PubMed:16968737). In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium (By similarity).
Interacts with HIP1 (By similarity).
Interacts with DENND1A, DENND1B and DENND1C (By similarity).
Interacts with OCRL (By similarity).
Interacts with ERBB2 (By similarity).
Interacts with FKBP6 (By similarity).
Interacts with CKAP5 and TACC3 forming the TACC3/ch-TOG/clathrin complex located at spindle inter-microtubules bridges; the complex implicates clathrin triskelions; TACC3 and CLTC are proposed to form a composite microtubule interaction surface (By similarity).
Interacts with ATG16L1 (via N-terminus) (By similarity).
Interacts with RFTN1; the interaction occurs in response to pathogens (By similarity).
Interacts with USP2 isoform 2 (By similarity).
By similarity1 PublicationBinary interactionsi
P11442
With | #Exp. | IntAct |
---|---|---|
OCRL [Q01968] from Homo sapiens. | 5 | EBI-397997,EBI-6148898 |
GO - Molecular functioni
- ankyrin binding Source: RGD
- clathrin light chain binding Source: RGD
- disordered domain specific binding Source: RGD
- heat shock protein binding Source: RGD
- low-density lipoprotein particle receptor binding Source: RGD
- protein C-terminus binding Source: RGD
- protein kinase binding Source: RGD
- ubiquitin-specific protease binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248464, 22 interactors |
CORUMi | P11442 |
DIPi | DIP-36966N |
IntActi | P11442, 8 interactors |
MINTi | P11442 |
STRINGi | 10116.ENSRNOP00000005987 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P11442 |
SMRi | P11442 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P11442 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 537 – 683 | CHCR 1Add BLAST | 147 | |
Repeati | 686 – 828 | CHCR 2Add BLAST | 143 | |
Repeati | 833 – 972 | CHCR 3Add BLAST | 140 | |
Repeati | 979 – 1124 | CHCR 4Add BLAST | 146 | |
Repeati | 1128 – 1269 | CHCR 5Add BLAST | 142 | |
Repeati | 1274 – 1420 | CHCR 6Add BLAST | 147 | |
Repeati | 1423 – 1566 | CHCR 7Add BLAST | 144 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 479 | Globular terminal domainAdd BLAST | 478 | |
Regioni | 24 – 67 | WD40-like repeat 1Add BLAST | 44 | |
Regioni | 68 – 107 | WD40-like repeat 2Add BLAST | 40 | |
Regioni | 108 – 149 | WD40-like repeat 3Add BLAST | 42 | |
Regioni | 150 – 195 | WD40-like repeat 4Add BLAST | 46 | |
Regioni | 196 – 257 | WD40-like repeat 5Add BLAST | 62 | |
Regioni | 258 – 301 | WD40-like repeat 6Add BLAST | 44 | |
Regioni | 302 – 330 | WD40-like repeat 7Add BLAST | 29 | |
Regioni | 449 – 465 | Binding site for the uncoating ATPase, involved in lattice disassemblySequence analysisAdd BLAST | 17 | |
Regioni | 480 – 523 | Flexible linkerAdd BLAST | 44 | |
Regioni | 524 – 1675 | Heavy chain armAdd BLAST | 1152 | |
Regioni | 524 – 634 | Distal segmentAdd BLAST | 111 | |
Regioni | 639 – 1675 | Proximal segmentAdd BLAST | 1037 | |
Regioni | 1213 – 1522 | Involved in binding clathrin light chainBy similarityAdd BLAST | 310 | |
Regioni | 1550 – 1675 | TrimerizationBy similarityAdd BLAST | 126 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0985, Eukaryota |
InParanoidi | P11442 |
OrthoDBi | 17940at2759 |
PhylomeDBi | P11442 |
Family and domain databases
Gene3Di | 1.25.40.10, 4 hits 2.130.10.110, 1 hit |
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR000547, Clathrin_H-chain/VPS_repeat IPR015348, Clathrin_H-chain_linker_core IPR016025, Clathrin_H-chain_N IPR022365, Clathrin_H-chain_propeller_rpt IPR016341, Clathrin_heavy_chain IPR011990, TPR-like_helical_dom_sf |
Pfami | View protein in Pfam PF00637, Clathrin, 7 hits PF09268, Clathrin-link, 1 hit PF01394, Clathrin_propel, 5 hits |
PIRSFi | PIRSF002290, Clathrin_H_chain, 1 hit |
SMARTi | View protein in SMART SM00299, CLH, 7 hits |
SUPFAMi | SSF48371, SSF48371, 6 hits SSF50989, SSF50989, 1 hit |
PROSITEi | View protein in PROSITE PS50236, CHCR, 7 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAQILPIRFQ EHLQLQNLGI NPANIGFSTL TMESDKFICI REKVGEQAQV
60 70 80 90 100
VIIDMNDPSN PIRRPISADS AIMNPASKVI ALKAGKTLQI FNIEMKSKMK
110 120 130 140 150
AHTMTDDVTF WKWISLNTVA LVTDNAVYHW SMEGESQPVK MFDRHSSLAG
160 170 180 190 200
CQIINYRTDA KQKWLLLTGI SAQQNRVVGA MQLYSVDRKV SQPIEGHAAS
210 220 230 240 250
FAQFKMEGNA EESTLFCFAV RGQAGGKLHI IEVGTPPTGN QPFPKKAVDV
260 270 280 290 300
FFPPEAQNDF PVAMQISEKH DVVFLITKYG YIHLYDLETG TCIYMNRISG
310 320 330 340 350
ETIFVTAPHE ATAGIIGVNR KGQVLSVCVE EENIIPYITN VLQNPDLALR
360 370 380 390 400
MAVRNNLAGA EELFARKFNA LFAQGNYSEA AKVAANAPKG ILRTPDTIRR
410 420 430 440 450
FQSVPAQPGQ TSPLLQYFGI LLDQGQLNKY ESLELCRPVL QQGRKQLLEK
460 470 480 490 500
WLKEDKLECS EELGDLVKSV DPTLALSVYL RANVPNKVIQ CFAETGQVQK
510 520 530 540 550
IVLYAKKVGY TPDWIFLLRN VMRISPDQGQ QFAQMLVQDE EPLADITQIV
560 570 580 590 600
DVFMEYNLIQ QCTAFLLDAL KNNRPSEGPL QTRLLEMNLM HAPQVADAIL
610 620 630 640 650
GNQMFTHYDR AHIAQLCEKA GLLQRALEHF TDLYDIKRAV VHTHLLNPEW
660 670 680 690 700
LVNYFGSLSV EDSLECLRAM LSANIRQNLQ IWVQVASKYH EQLSTQSLIE
710 720 730 740 750
LFESFKSFEG LFYFLGSIVN FSQDPDVHFK YIQAACKTGQ IKEVERICRE
760 770 780 790 800
SNCYDPERVK NFLKEAKLTD QLPLIIVCDR FDFVHDLVLY LYRNSLQKYI
810 820 830 840 850
EIYVQKVNPS RLPVVIGGLL DVDCSEDVIK NLILVVRGQF STDELVAEVE
860 870 880 890 900
KRNRLKLLLP WLEARIHEGC EEPATHNALA KIYIDSNNNP ERFLRENPYY
910 920 930 940 950
DSRVVGKYCE KRDPHLACVA YERGQCDLEL INVCNENSLF KSLSRYLVRR
960 970 980 990 1000
KDPELWGSVL LESNPYRRPL IDQVVQTALS ETQDPEEVSV TVKAFMTADL
1010 1020 1030 1040 1050
PNELIELLEK IVLDNSVFSE HRNLQNLLIL TAIKADRTRV MEYINRLDNY
1060 1070 1080 1090 1100
DAPDIANIAI SNELFEEAFA IFRKFDVNTS AVQVLIEHIG NLDRAYEFAE
1110 1120 1130 1140 1150
RCNEPAVWSQ LAKAQLQKGM VKEAIDSYIK ADDPSSYMEV VQAANTSGNW
1160 1170 1180 1190 1200
EELVKYLQMA RKKARESYVE TELIFALAKT NRLAELEEFI NGPNNAHIQQ
1210 1220 1230 1240 1250
VGDRCYDEKM YDAAKLLYNN VSNFGRLAST LVHLGEYQAA VDGARKANST
1260 1270 1280 1290 1300
RTWKEVCFAC VDGKEFRLAQ MCGLHIVVHA DELEELINYY QDRGYFEELI
1310 1320 1330 1340 1350
TMLEAALGLE RAHMGMFTEL AILYSKFKPQ KMREHLELFW SRVNIPKVLR
1360 1370 1380 1390 1400
AAEQAHLWAE LVFLYDKYEE YDNAIITMMN HPTDAWKEGQ FKDIITKVAN
1410 1420 1430 1440 1450
VELYYKAIQF YLEFKPLLLN DLLMVLSPRL AHTRAVNYFS KVKQLPLVKP
1460 1470 1480 1490 1500
YLRSVQNHNN KSVNESLNNL FITEEDYQAL RTSIDAYDNF DNISLAQRLE
1510 1520 1530 1540 1550
KHELIEFRRI AAYLFKGNNR WKQSVELCKK DSLYKDAMQY ASESKDTELA
1560 1570 1580 1590 1600
EELLQWFLQE EKRECFGACL FTCYDLLRPD VVLETAWRHN IMDFAMPYFI
1610 1620 1630 1640 1650
QVMKEYLTKV DKLDASESLR KEEEQATETQ PIVYGQPQLM LTAGPSVAVP
1660 1670
PQAPFGYGYT APPYGQPQPG FGYSM
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1M779 | F1M779_RAT | Clathrin heavy chain | Cltc | 1,664 | Annotation score: | ||
A0A8I5Y9U9 | A0A8I5Y9U9_RAT | Clathrin heavy chain 1 | Cltc | 1,621 | Annotation score: | ||
A0A8I6A5W9 | A0A8I6A5W9_RAT | Clathrin heavy chain 1 | Cltc | 1,616 | Annotation score: | ||
A0A8I6AGZ0 | A0A8I6AGZ0_RAT | Clathrin heavy chain 1 | Cltc | 1,651 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03583 mRNA Translation: AAA40874.1 |
PIRi | A39941, LRRTH |
RefSeqi | NP_062172.1, NM_019299.1 |
Genome annotation databases
GeneIDi | 54241 |
KEGGi | rno:54241 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03583 mRNA Translation: AAA40874.1 |
PIRi | A39941, LRRTH |
RefSeqi | NP_062172.1, NM_019299.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BPO | X-ray | 2.60 | A/B/C | 1-494 | [»] | |
1C9I | X-ray | 2.90 | A/B | 1-359 | [»] | |
1C9L | X-ray | 2.90 | A/B | 3-359 | [»] | |
AlphaFoldDBi | P11442 | |||||
SMRi | P11442 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 248464, 22 interactors |
CORUMi | P11442 |
DIPi | DIP-36966N |
IntActi | P11442, 8 interactors |
MINTi | P11442 |
STRINGi | 10116.ENSRNOP00000005987 |
PTM databases
iPTMneti | P11442 |
PhosphoSitePlusi | P11442 |
2D gel databases
World-2DPAGEi | 0004:P11442 |
Proteomic databases
jPOSTi | P11442 |
PaxDbi | P11442 |
PRIDEi | P11442 |
Genome annotation databases
GeneIDi | 54241 |
KEGGi | rno:54241 |
Organism-specific databases
CTDi | 1213 |
RGDi | 2364, Cltc |
Phylogenomic databases
eggNOGi | KOG0985, Eukaryota |
InParanoidi | P11442 |
OrthoDBi | 17940at2759 |
PhylomeDBi | P11442 |
Enzyme and pathway databases
Reactomei | R-RNO-177504, Retrograde neurotrophin signalling R-RNO-190873, Gap junction degradation R-RNO-196025, Formation of annular gap junctions R-RNO-2132295, MHC class II antigen presentation R-RNO-432720, Lysosome Vesicle Biogenesis R-RNO-432722, Golgi Associated Vesicle Biogenesis R-RNO-437239, Recycling pathway of L1 R-RNO-5099900, WNT5A-dependent internalization of FZD4 R-RNO-5140745, WNT5A-dependent internalization of FZD2, FZD5 and ROR2 R-RNO-8856825, Cargo recognition for clathrin-mediated endocytosis R-RNO-8856828, Clathrin-mediated endocytosis R-RNO-8866427, VLDLR internalisation and degradation R-RNO-8964038, LDL clearance R-RNO-9013420, RHOU GTPase cycle R-RNO-9013424, RHOV GTPase cycle |
Miscellaneous databases
EvolutionaryTracei | P11442 |
PROi | PR:P11442 |
Family and domain databases
Gene3Di | 1.25.40.10, 4 hits 2.130.10.110, 1 hit |
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR000547, Clathrin_H-chain/VPS_repeat IPR015348, Clathrin_H-chain_linker_core IPR016025, Clathrin_H-chain_N IPR022365, Clathrin_H-chain_propeller_rpt IPR016341, Clathrin_heavy_chain IPR011990, TPR-like_helical_dom_sf |
Pfami | View protein in Pfam PF00637, Clathrin, 7 hits PF09268, Clathrin-link, 1 hit PF01394, Clathrin_propel, 5 hits |
PIRSFi | PIRSF002290, Clathrin_H_chain, 1 hit |
SMARTi | View protein in SMART SM00299, CLH, 7 hits |
SUPFAMi | SSF48371, SSF48371, 6 hits SSF50989, SSF50989, 1 hit |
PROSITEi | View protein in PROSITE PS50236, CHCR, 7 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | CLH1_RAT | |
Accessioni | P11442Primary (citable) accession number: P11442 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1989 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 180 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families