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Protein

Nuclear transition protein 2

Gene

Tnp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Plays a key role in the replacement of histones to protamine in the elongating spermatids of mammals (PubMed:15163613, PubMed:15189834, PubMed:15083521, PubMed:28366643). In condensing spermatids, loaded onto the nucleosomes, where it promotes the recruitment and processing of protamines, which are responsible for histone eviction (PubMed:28366643). The histone H2AFB1-HIST1H2BA/TH2B dimer is required for loading of TNP2 onto chromatin (PubMed:28366643).4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12ZincBy similarity1
Metal bindingi14ZincBy similarity1
Metal bindingi16ZincBy similarity1
Metal bindingi24ZincBy similarity1
Metal bindingi32ZincBy similarity1
Metal bindingi34ZincBy similarity1
Metal bindingi38ZincBy similarity1
Metal bindingi41ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processDifferentiation, Spermatogenesis
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear transition protein 2
Short name:
TP-2
Short name:
TP2
Gene namesi
Name:Tnp2Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:98785 Tnp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

Pathology & Biotechi

Disruption phenotypei

Male mice lacking both Tnp1 and Tnp2 are completely infertile, but protamine alone are capable of histone eviction (PubMed:15163613, PubMed:15189834, PubMed:15083521). A significant proportion of Prm2 remains unprocessed (PubMed:15163613, PubMed:15189834, PubMed:15083521). Chromatin in mature spermatozoa shows defects in density (PubMed:15189834, PubMed:15083521).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001914281 – 117Nuclear transition protein 2Add BLAST117

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei112PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP11378
PRIDEiP11378

PTM databases

iPTMnetiP11378
PhosphoSitePlusiP11378

Expressioni

Developmental stagei

Appears in elongating/condensing spermatids when histones are still detectable (PubMed:15163613). Coexpressed with H2afb1 during late spermiogenesis (PubMed:28366643).2 Publications

Gene expression databases

BgeeiENSMUSG00000043050 Expressed in 45 organ(s), highest expression level in testis
CleanExiMM_TNP2

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000053078

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi90 – 98Nuclear localization signalBy similarity9

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000008176
HOGENOMiHOG000065922
HOVERGENiHBG098551
InParanoidiP11378
KOiK19925
OMAiQQVYKTK

Family and domain databases

InterProiView protein in InterPro
IPR000678 TP2
PANTHERiPTHR17488 PTHR17488, 2 hits
PfamiView protein in Pfam
PF01254 TP2, 2 hits
PROSITEiView protein in PROSITE
PS00970 TP2_1, 1 hit
PS00971 TP2_2, 1 hit

Sequencei

Sequence statusi: Complete.

P11378-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDTKMQSLPT THPHPHSSSR PQSHTSNQCN QCTCSHHCRS CSQAGHAGSS
60 70 80 90 100
SSPSPGPPMK HPKPSVHSRH SPARPSHRGS CPKNRKTFEG KVSKRKAVRR
110
RKRTHRAKRR SSGRRYK
Length:117
Mass (Da):13,117
Last modified:June 7, 2017 - v3
Checksum:iF5692E66E5F3F2CA
GO

Sequence cautioni

The sequence AAA40468 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111S → T in AAA40469 (PubMed:2249785).1
Sequence conflicti111S → T in AAA40467 (PubMed:3693351).1
Sequence conflicti111S → T in AAA40468 (PubMed:3693351).1
Sequence conflicti111S → T in CAA87413 (PubMed:8720108).1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60254 Genomic DNA Translation: AAA40469.1
J03494 mRNA Translation: AAA40467.1
J03494 mRNA Translation: AAA40468.1 Different initiation.
Z47352 Genomic DNA Translation: CAA87413.1
AK005689 mRNA Translation: BAB24187.1
CT010583 Genomic DNA No translation available.
CH466521 Genomic DNA Translation: EDK97324.1
BC092529 mRNA Translation: AAH92529.1
CCDSiCCDS27953.1
PIRiS14529
RefSeqiNP_038722.3, NM_013694.4
UniGeneiMm.206798

Genome annotation databases

EnsembliENSMUST00000051297; ENSMUSP00000053078; ENSMUSG00000043050
GeneIDi21959
KEGGimmu:21959
UCSCiuc007yee.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60254 Genomic DNA Translation: AAA40469.1
J03494 mRNA Translation: AAA40467.1
J03494 mRNA Translation: AAA40468.1 Different initiation.
Z47352 Genomic DNA Translation: CAA87413.1
AK005689 mRNA Translation: BAB24187.1
CT010583 Genomic DNA No translation available.
CH466521 Genomic DNA Translation: EDK97324.1
BC092529 mRNA Translation: AAH92529.1
CCDSiCCDS27953.1
PIRiS14529
RefSeqiNP_038722.3, NM_013694.4
UniGeneiMm.206798

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000053078

PTM databases

iPTMnetiP11378
PhosphoSitePlusiP11378

Proteomic databases

PaxDbiP11378
PRIDEiP11378

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051297; ENSMUSP00000053078; ENSMUSG00000043050
GeneIDi21959
KEGGimmu:21959
UCSCiuc007yee.1 mouse

Organism-specific databases

CTDi7142
MGIiMGI:98785 Tnp2

Phylogenomic databases

GeneTreeiENSGT00390000008176
HOGENOMiHOG000065922
HOVERGENiHBG098551
InParanoidiP11378
KOiK19925
OMAiQQVYKTK

Miscellaneous databases

ChiTaRSiTnp2 mouse
PROiPR:P11378
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000043050 Expressed in 45 organ(s), highest expression level in testis
CleanExiMM_TNP2

Family and domain databases

InterProiView protein in InterPro
IPR000678 TP2
PANTHERiPTHR17488 PTHR17488, 2 hits
PfamiView protein in Pfam
PF01254 TP2, 2 hits
PROSITEiView protein in PROSITE
PS00970 TP2_1, 1 hit
PS00971 TP2_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSTP2_MOUSE
AccessioniPrimary (citable) accession number: P11378
Secondary accession number(s): Q91XK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: June 7, 2017
Last modified: September 12, 2018
This is version 123 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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