Tnp2

Functionⁱ

Plays a key role in the replacement of histones to protamine in the elongating spermatids of mammals (PubMed:15163613, PubMed:15189834, PubMed:15083521, PubMed:28366643). In condensing spermatids, loaded onto the nucleosomes, where it promotes the recruitment and processing of protamines, which are responsible for histone eviction (PubMed:28366643). The histone H2AFB1-HIST1H2BA/TH2B dimer is required for loading of TNP2 onto chromatin (PubMed:28366643).4 Publications

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	12	ZincBy similarity	1
Metal bindingⁱ	14	ZincBy similarity	1
Metal bindingⁱ	16	ZincBy similarity	1
Metal bindingⁱ	24	ZincBy similarity	1
Metal bindingⁱ	32	ZincBy similarity	1
Metal bindingⁱ	34	ZincBy similarity	1
Metal bindingⁱ	38	ZincBy similarity	1
Metal bindingⁱ	41	ZincBy similarity	1

GO - Molecular functionⁱ

DNA binding Source: UniProtKB-KW
zinc ion binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

acrosome reaction
Source: MGI
Inferred from mutant phenotypeⁱ
- 11385107
binding of sperm to zona pellucida
Source: MGI
Inferred from genetic interactionⁱ
- 15685642
flagellated sperm motility
Source: MGI
Inferred from genetic interactionⁱ
- 15051954
- 15685642
multicellular organism development Source: UniProtKB-KW
penetration of zona pellucida
Source: MGI
Inferred from mutant phenotypeⁱ
- 11385107
positive regulation of protein processing
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 28366643
spermatogenesis
Source: MGI
Inferred from mutant phenotypeⁱ
- 11385107
spermatogenesis, exchange of chromosomal proteins
Source: UniProtKB
Inferred from mutant phenotypeⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Developmental protein, DNA-binding
Biological process	Differentiation, Spermatogenesis
Ligand	Metal-binding, Zinc

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Nuclear transition protein 2 Short name: TP-2 Short name: TP2
Gene namesⁱ	Name:Tnp2Imported
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 16

Organism-specific databases

MGIⁱ	MGI:98785 Tnp2

Keywords - Cellular componentⁱ

Chromosome, Nucleosome core, Nucleus

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Male mice lacking both Tnp1 and Tnp2 are completely infertile, but protamine alone are capable of histone eviction (PubMed:15163613, PubMed:15189834, PubMed:15083521). A significant proportion of Prm2 remains unprocessed (PubMed:15163613, PubMed:15189834, PubMed:15083521). Chromatin in mature spermatozoa shows defects in density (PubMed:15189834, PubMed:15083521).3 Publications

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000191428	1 – 117	Nuclear transition protein 2Add BLAST		117

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	112	PhosphoserineBy similarity		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	P11378
PRIDEⁱ	P11378

PTM databases

iPTMnetⁱ	P11378
PhosphoSitePlusⁱ	P11378

Expressionⁱ

Developmental stageⁱ

Appears in elongating/condensing spermatids when histones are still detectable (PubMed:15163613). Coexpressed with H2afb1 during late spermiogenesis (PubMed:28366643).2 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000043050 Expressed in 45 organ(s), highest expression level in testis
CleanExⁱ	MM_TNP2

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000053078

STRINGⁱ

10090.ENSMUSP00000053078

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	90 – 98	Nuclear localization signalBy similarity		9

Sequence similaritiesⁱ

Belongs to the nuclear transition protein 2 family.Curated

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000008176
HOGENOMⁱ	HOG000065922
HOVERGENⁱ	HBG098551
InParanoidⁱ	P11378
KEGG Orthology (KO) More...KOⁱ	K19925
OMAⁱ	QQVYKTK

Family and domain databases

InterProⁱ	View protein in InterPro IPR000678 TP2
PANTHERⁱ	PTHR17488 PTHR17488, 2 hits
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01254 TP2, 2 hits
PROSITEⁱ	View protein in PROSITE PS00970 TP2_1, 1 hit PS00971 TP2_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P11378-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDTKMQSLPT THPHPHSSSR PQSHTSNQCN QCTCSHHCRS CSQAGHAGSS 
        60         70         80         90        100
SSPSPGPPMK HPKPSVHSRH SPARPSHRGS CPKNRKTFEG KVSKRKAVRR 
       110 
RKRTHRAKRR SSGRRYK

Length:117

Mass (Da):13,117

Last modified:June 7, 2017 - v3

Checksum:ⁱF5692E66E5F3F2CA

GO

Sequence cautionⁱ

The sequence AAA40468 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	111	S → T in AAA40469 (PubMed:2249785).	1
Sequence conflictⁱ	111	S → T in AAA40467 (PubMed:3693351).	1
Sequence conflictⁱ	111	S → T in AAA40468 (PubMed:3693351).	1
Sequence conflictⁱ	111	S → T in CAA87413 (PubMed:8720108).	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M60254 Genomic DNA Translation: AAA40469.1 J03494 mRNA Translation: AAA40467.1 J03494 mRNA Translation: AAA40468.1 Different initiation. Z47352 Genomic DNA Translation: CAA87413.1 AK005689 mRNA Translation: BAB24187.1 CT010583 Genomic DNA No translation available. CH466521 Genomic DNA Translation: EDK97324.1 BC092529 mRNA Translation: AAH92529.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS27953.1
PIRⁱ	S14529
NCBI Reference Sequences More...RefSeqⁱ	NP_038722.3, NM_013694.4
UniGeneⁱ	Mm.206798

Genome annotation databases

Ensemblⁱ	ENSMUST00000051297; ENSMUSP00000053078; ENSMUSG00000043050
GeneIDⁱ	21959
KEGGⁱ	mmu:21959
UCSC genome browser More...UCSCⁱ	uc007yee.1 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P11378	Nuclear transition protein 2		RAT		114	UniRef90_P11101
UPI000A318283		MUSCR		117
UPI000A312B9F		MUSPA		103

Protein	Similar proteins		Species	Score	Length	Source
P11378	Nuclear transition protein 2		RAT		114	UniRef50_P11101
UPI000A318283		MUSCR		117
UPI000A312B9F		MUSPA		103
UPI000B4FA44E		MERUN		138
UPI000332F68F		JACJA		145

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M60254 Genomic DNA Translation: AAA40469.1 J03494 mRNA Translation: AAA40467.1 J03494 mRNA Translation: AAA40468.1 Different initiation. Z47352 Genomic DNA Translation: CAA87413.1 AK005689 mRNA Translation: BAB24187.1 CT010583 Genomic DNA No translation available. CH466521 Genomic DNA Translation: EDK97324.1 BC092529 mRNA Translation: AAH92529.1
CCDSⁱ	CCDS27953.1
PIRⁱ	S14529
RefSeqⁱ	NP_038722.3, NM_013694.4
UniGeneⁱ	Mm.206798

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000053078

STRINGⁱ

10090.ENSMUSP00000053078

PTM databases

iPTMnetⁱ	P11378
PhosphoSitePlusⁱ	P11378

Proteomic databases

PaxDbⁱ	P11378
PRIDEⁱ	P11378

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000051297; ENSMUSP00000053078; ENSMUSG00000043050
GeneIDⁱ	21959
KEGGⁱ	mmu:21959
UCSCⁱ	uc007yee.1 mouse

Organism-specific databases

CTDⁱ	7142
MGIⁱ	MGI:98785 Tnp2

Phylogenomic databases

GeneTreeⁱ	ENSGT00390000008176
HOGENOMⁱ	HOG000065922
HOVERGENⁱ	HBG098551
InParanoidⁱ	P11378
KOⁱ	K19925
OMAⁱ	QQVYKTK

Miscellaneous databases

ChiTaRSⁱ	Tnp2 mouse
Protein Ontology More...PROⁱ	PR:P11378
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000043050 Expressed in 45 organ(s), highest expression level in testis
CleanExⁱ	MM_TNP2

Family and domain databases

InterProⁱ	View protein in InterPro IPR000678 TP2
PANTHERⁱ	PTHR17488 PTHR17488, 2 hits
Pfamⁱ	View protein in Pfam PF01254 TP2, 2 hits
PROSITEⁱ	View protein in PROSITE PS00970 TP2_1, 1 hit PS00971 TP2_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	STP2_MOUSE
Accessionⁱ	P11378Primary (citable) accession number: P11378 Secondary accession number(s): Q91XK4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
	Last sequence update:	June 7, 2017
	Last modified:	September 12, 2018
	This is version 123 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P11378 (STP2_MOUSE)

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Nuclear transition protein 2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ